##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933975.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	796479
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.386194739597656	33.0	31.0	34.0	31.0	34.0
2	32.616114172501725	34.0	31.0	34.0	31.0	34.0
3	32.66985319135846	34.0	31.0	34.0	31.0	34.0
4	36.04803767582071	37.0	35.0	37.0	35.0	37.0
5	36.099889639274856	37.0	35.0	37.0	35.0	37.0
6	35.73394025454532	37.0	35.0	37.0	35.0	37.0
7	36.050163281141124	37.0	35.0	37.0	35.0	37.0
8	36.055363669349724	37.0	36.0	37.0	35.0	37.0
9	37.924775166702446	39.0	38.0	39.0	35.0	39.0
10	37.57087129729723	39.0	37.0	39.0	35.0	39.0
11	37.497788391156575	39.0	37.0	39.0	35.0	39.0
12	37.363625406319564	39.0	37.0	39.0	35.0	39.0
13	37.300665805375914	39.0	37.0	39.0	34.0	39.0
14	38.511508778009215	40.0	38.0	41.0	34.0	41.0
15	38.572827406623404	40.0	38.0	41.0	35.0	41.0
16	38.61665153757977	40.0	38.0	41.0	35.0	41.0
17	38.602490461142104	40.0	38.0	41.0	35.0	41.0
18	38.56488620541157	40.0	38.0	41.0	35.0	41.0
19	38.59609230124084	40.0	38.0	41.0	35.0	41.0
20	38.549506013341215	40.0	38.0	41.0	34.0	41.0
21	38.47364211736907	40.0	38.0	41.0	34.0	41.0
22	38.45457067920184	40.0	38.0	41.0	34.0	41.0
23	38.417626830085915	40.0	38.0	41.0	34.0	41.0
24	38.392390759831706	40.0	37.0	41.0	34.0	41.0
25	38.30810981833796	40.0	37.0	41.0	34.0	41.0
26	38.255567315647994	40.0	37.0	41.0	34.0	41.0
27	38.22128015930112	40.0	37.0	41.0	34.0	41.0
28	38.09185427362178	40.0	37.0	41.0	34.0	41.0
29	38.10709133574143	40.0	37.0	41.0	34.0	41.0
30	38.02247140225919	40.0	37.0	41.0	34.0	41.0
31	37.91028639800924	40.0	37.0	41.0	34.0	41.0
32	37.827161795854	40.0	36.0	41.0	33.0	41.0
33	37.797419643204655	40.0	37.0	41.0	33.0	41.0
34	37.77993518975391	40.0	37.0	41.0	33.0	41.0
35	37.74091721187878	40.0	37.0	41.0	33.0	41.0
36	37.645329004280086	40.0	36.0	41.0	33.0	41.0
37	37.48984216784121	40.0	36.0	41.0	33.0	41.0
38	37.41846552137596	40.0	36.0	41.0	33.0	41.0
39	37.454054658063804	40.0	36.0	41.0	33.0	41.0
40	37.37733574896513	40.0	36.0	41.0	33.0	41.0
41	37.26659083290332	39.0	35.0	41.0	32.0	41.0
42	37.25435573317062	39.0	35.0	41.0	32.0	41.0
43	37.162623245559516	39.0	35.0	41.0	32.0	41.0
44	37.037781284880076	39.0	35.0	41.0	32.0	41.0
45	37.04639042586182	39.0	35.0	41.0	32.0	41.0
46	36.9439056145862	39.0	35.0	41.0	32.0	41.0
47	36.842177885418195	39.0	35.0	41.0	31.0	41.0
48	36.79578997060814	39.0	35.0	41.0	31.0	41.0
49	36.78830578081782	39.0	35.0	41.0	31.0	41.0
50	36.706414105079986	39.0	35.0	41.0	31.0	41.0
51	35.79384264996315	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	15.0
10	16.0
11	16.0
12	6.0
13	11.0
14	9.0
15	25.0
16	33.0
17	45.0
18	93.0
19	178.0
20	280.0
21	461.0
22	819.0
23	1158.0
24	1822.0
25	2706.0
26	3833.0
27	4915.0
28	6036.0
29	7116.0
30	9245.0
31	12226.0
32	16146.0
33	22833.0
34	43593.0
35	57613.0
36	57034.0
37	92523.0
38	181033.0
39	274460.0
40	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.94546246668148	4.79523000606419	55.78841375604379	17.47089377121054
2	19.120780334446984	4.353536000321415	53.633303577369894	22.892380087861703
3	19.61169095481488	4.333698691365371	53.36499769611001	22.689612657709745
4	19.069429325820266	4.483734034418987	52.93159016119697	23.51524647856378
5	18.39332863766653	5.524690544257915	51.60387153961372	24.47810927846183
6	21.04562706612478	6.281772651884104	53.16273247631137	19.509867805679747
7	81.97152718401867	2.400942146622824	9.913255716723228	5.714274952635287
8	82.81674720865207	2.9944292316558254	8.27228338725817	5.916540172433924
9	77.56086475600738	5.0337799238900205	11.16149954989397	6.24385577020863
10	36.74158389612281	29.446350751243912	19.134716671751548	14.67734868088173
11	25.275117109176765	25.591384079178486	28.83214748913656	20.30135132250819
12	24.842211784617046	22.083695866432134	31.348974674787407	21.72511767416341
13	23.144489685227107	23.24154183600572	32.57072691182065	21.043241566946524
14	18.933706977836202	26.004954305135474	30.741676805038175	24.319661911990146
15	17.451809777784476	26.05002768434573	34.66067529715159	21.83748724071821
16	19.556698921126607	25.954482164627063	31.061459247513117	23.42735966673321
17	20.10373154847774	26.05153431540568	29.01896974057069	24.825764395545896
18	20.306498978629694	25.12884834377303	31.941206233937113	22.623446443660157
19	21.194281330706772	25.800931349100225	30.03067249732887	22.97411482286413
20	22.856346495011167	26.527629730350704	30.23030111277259	20.385722661865536
21	23.505202271497428	26.788904666664155	29.89733564852306	19.808557413315352
22	22.143082240711934	24.413073037707207	29.426011231934552	24.017833489646307
23	21.412617281811574	25.236195806794655	28.57802905035789	24.773157861035884
24	21.330003678690836	23.917516971571125	30.23080332312591	24.521676026612127
25	20.086530843876613	26.219272573413736	27.952777160477556	25.7414194222321
26	21.57470567334481	27.243907246769844	27.149993910699465	24.03139316918588
27	22.108178621156366	26.86787724472334	28.96937646818058	22.054567665939718
28	18.68297845894242	25.084528280092762	32.58039446112201	23.652098799842808
29	20.772047976155054	22.354889457223607	29.946803368324844	26.926259198296503
30	21.7391795640563	25.003923518385296	27.98730412226813	25.26959279529027
31	20.97468985371868	28.956821209347645	26.635479403725647	23.433009533208033
32	21.244879023803513	28.18994599983176	25.921336281308104	24.64383869505662
33	21.337160176225613	25.980722655587908	28.04882489054953	24.63329227763695
34	19.459521217759665	25.012461094391693	29.739892702758013	25.788124985090633
35	21.491338754694098	24.82350444895597	27.588297996557348	26.09685879979259
36	23.612173076754065	25.55535048632795	27.923146749631815	22.909329687286167
37	20.365885352909494	26.947477585724165	30.80194204743628	21.88469501393006
38	18.67845856576256	26.370437889762314	29.78496608196826	25.16613746250686
39	19.206783857452614	24.18218182776947	30.179326761910865	26.431707552867056
40	21.219015190607664	25.889445923872444	28.317632982162742	24.57390590335715
41	19.04595099180267	27.55515211323839	27.855097246757293	25.54379964820165
42	21.07224421485061	24.411943064412245	29.428145625936153	25.08766709480099
43	22.37648450241626	22.52526431958658	30.426916466096404	24.67133471190075
44	20.94543610063793	22.193052170867027	29.043201390118256	27.81831033837678
45	21.64363404433764	22.954528619084748	27.021302507661847	28.380534828915767
46	22.525766529939897	23.905212817914848	29.20353204541488	24.365488606730374
47	18.765592062063156	24.217838762855017	32.288233588079535	24.72833558700229
48	18.62208545360267	22.874802725495588	32.67958100590222	25.82353081499952
49	20.769285819211806	21.5135615628284	32.75240150713327	24.964751110826526
50	20.96816111912555	21.925625157725438	29.458152694546875	27.648061028602132
51	18.96722951892015	22.291610952705597	27.633622480944254	31.10753704743
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	420.5
2	725.0
3	1709.0
4	2693.0
5	1879.5
6	1066.0
7	1089.0
8	1112.0
9	1260.5
10	1409.0
11	1318.5
12	1228.0
13	1213.0
14	1198.0
15	1234.5
16	1271.0
17	1319.0
18	1367.0
19	1631.5
20	1896.0
21	2006.0
22	2116.0
23	2512.5
24	2909.0
25	3398.0
26	5617.0
27	7347.0
28	8706.5
29	10066.0
30	12219.0
31	14372.0
32	16120.5
33	17869.0
34	19339.5
35	20810.0
36	22584.5
37	24359.0
38	26822.0
39	29285.0
40	32701.0
41	36117.0
42	41219.0
43	46321.0
44	52995.0
45	59669.0
46	68025.0
47	76381.0
48	89713.0
49	103045.0
50	98916.0
51	94787.0
52	79435.0
53	64083.0
54	53671.0
55	43259.0
56	37305.5
57	31352.0
58	30062.5
59	28773.0
60	25004.5
61	21236.0
62	18541.0
63	15846.0
64	12628.0
65	9410.0
66	8169.5
67	6929.0
68	5924.0
69	4919.0
70	3947.5
71	2976.0
72	2596.0
73	2216.0
74	1651.0
75	749.5
76	413.0
77	352.5
78	292.0
79	191.5
80	91.0
81	119.0
82	147.0
83	79.0
84	11.0
85	10.0
86	9.0
87	8.0
88	7.0
89	4.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	796479.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.727158354098908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.8275997800647	19.86594641086329
2	13.58839653702477	8.078888312688493
3	5.821819870351982	5.191984835849789
4	2.9107024016208305	3.461076448585537
5	1.6960468717958748	2.520932696692503
6	1.068930352150601	1.906575710873018
7	0.7614481446818707	1.5844982702774952
8	0.5421509641014103	1.2893286049336015
9	0.43229456455638227	1.156580007856543
>10	6.034926575074005	42.825758458620484
>50	0.24306381769852423	4.460694754326552
>100	0.06493093160951278	3.4290471683688977
>500	0.0038445946347737832	0.7521477753675139
>1k	0.0029902402714907204	1.930847024221349
>5k	8.54354363283063E-4	1.5456935204749924
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	6502	0.8163429293176594	No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGCT	5670	0.7118831758276113	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	4430	0.5561979662991742	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	3647	0.457890289637266	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTG	1850	0.2322722884093617	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTG	1556	0.1953598274405226	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1469	0.18443675225586612	No Hit
GCCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTG	1144	0.1436321610488161	No Hit
TGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTG	1109	0.13923782045728764	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCT	950	0.11927495891291547	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7489211893847798	0.0
2	0.0	0.0	0.0	2.7444540282920205	0.0
3	0.0	0.0	0.0	3.91987736023172	0.0
4	0.0	0.0	0.0	5.003145092337651	0.0
5	0.0	0.0	0.0	6.631436610381441	0.0
6	0.0	0.0	0.0	7.564417894257099	0.0
7	0.0	0.0	0.0	8.479570710589984	0.0
8	0.0	0.0	0.0	9.87395775657613	0.0
9	0.0	0.0	0.0	10.481506731502023	0.0
10	0.0	0.0	0.0	11.719706357606416	0.0
11	0.0	0.0	0.0	14.168358487794405	0.0
12	0.0	0.0	0.0	16.39139261675449	0.0
13	0.0	0.0	0.0	17.131148467191224	0.0
14	0.0	0.0	0.0	17.376854882551832	0.0
15	0.0	0.0	0.0	17.774228824614333	0.0
16	0.0	0.0	0.0	18.714868816378083	0.0
17	0.0	0.0	0.0	19.96662812202205	0.0
18	0.0	0.0	0.0	21.338666807285566	0.0
19	0.0	0.0	0.0	22.291234294940608	0.0
20	0.0	0.0	0.0	23.317501151945	0.0
21	1.2555258832938471E-4	0.0	0.0	24.748172895958337	0.0
22	6.277629416469235E-4	0.0	0.0	26.193283187629554	0.0
23	6.277629416469235E-4	0.0	0.0	27.43286389220557	0.0
24	6.277629416469235E-4	0.0	0.0	28.475829243457767	0.0
25	6.277629416469235E-4	0.0	0.0	29.41785031369314	0.0
26	6.277629416469235E-4	0.0	0.0	30.192635336273774	0.0
27	6.277629416469235E-4	0.0	0.0	30.99102424546033	0.0
28	6.277629416469235E-4	0.0	0.0	31.788032076175266	0.0
29	6.277629416469235E-4	0.0	0.0	32.6085182409078	0.0
30	6.277629416469235E-4	0.0	0.0	33.60854460695135	0.0
31	6.277629416469235E-4	0.0	0.0	34.480507332898924	0.0
32	6.277629416469235E-4	0.0	0.0	35.28077953091042	0.0
33	6.277629416469235E-4	0.0	0.0	36.042632636893124	0.0
34	6.277629416469235E-4	0.0	0.0	36.78238848732986	0.0
35	6.277629416469235E-4	0.0	0.0	37.61467659536535	0.0
36	6.277629416469235E-4	0.0	0.0	38.37025207193159	0.0
37	7.533155299763082E-4	0.0	0.0	39.1159088940198	0.0
38	7.533155299763082E-4	0.0	0.0	39.85390700821993	0.0
39	7.533155299763082E-4	0.0	0.0	40.67301209448083	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAG	30	2.1646592E-6	45.000004	1
TAATACG	20	7.031982E-4	45.0	36
CGTCTAT	20	7.031982E-4	45.0	21
TCTAGCG	45	3.8380676E-10	45.0	1
TATTGCG	40	6.8102963E-9	45.0	1
GCGCTTA	25	3.8897233E-5	45.0	16
CGTCCGA	20	7.031982E-4	45.0	13
CGATAAG	20	7.031982E-4	45.0	32
CGAATAT	100	0.0	42.75	14
CATTTCG	55	6.002665E-11	40.909092	13
TTTTTCG	365	0.0	40.684933	1
TTCGCAG	50	1.0804797E-9	40.5	1
TTGGGCA	910	0.0	40.3022	5
CTTGTTG	430	0.0	40.2907	1
TCGTTTG	275	0.0	40.090908	1
TTTGGGC	1800	0.0	40.000004	4
TTCTGCG	295	0.0	39.66102	1
CGTTTGG	460	0.0	39.619564	2
CGGGAAT	325	0.0	39.46154	6
TTGTGCG	160	0.0	39.375	1
>>END_MODULE