FastQCFastQC Report
Sat 14 Jan 2017
SRR2933974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761260
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC59990.7880356251477812Illumina PCR Primer Index 11 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT52600.6909597246669994Illumina PCR Primer Index 11 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC49950.6561490161048787RNA PCR Primer, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC48040.6310590337072748RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC23530.3090928197987547No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC23050.3027874839082574No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG20940.27507027822294616No Hit
TCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG18560.2438063210992302Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16840.22121220082494808No Hit
CGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG13880.18232929616688123Illumina PCR Primer Index 11 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG12640.16604051178309645RNA PCR Primer, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG11890.15618842445419437RNA PCR Primer, Index 14 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT10190.13385702650868297RNA PCR Primer, Index 14 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG7910.10390668102882065RNA PCR Primer, Index 14 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC7880.10351259753566455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG650.045.0000041
GTCAACG351.2112105E-745.0000041
CGAGTTG207.031784E-445.06
GATCGAG253.88956E-545.011
TACGCGG253.88956E-545.02
TGATTCG207.031784E-445.01
CGCACTT800.045.035
GGCGATA253.88956E-545.08
CGATATT253.88956E-545.012
CGAGACT302.164532E-644.99999626
TACGTTG302.164532E-644.9999961
TTTACGT800.042.187526
CGTCCGG603.6379788E-1241.2499962
CGGTAGT556.002665E-1140.90909212
TGGGACG2200.040.9090926
CGCTAGA501.0804797E-940.524
GCCAACG451.9268555E-840.0000049
TTACACG1300.039.80769334
CGTTTGG4250.039.705882
TCTTGCG2500.039.6000021
Produced by FastQC (version 0.11.5)