##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933974.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	761260
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27685810367023	33.0	31.0	34.0	30.0	34.0
2	32.502820324199355	34.0	31.0	34.0	31.0	34.0
3	32.56678532958516	34.0	31.0	34.0	31.0	34.0
4	35.97524236134829	37.0	35.0	37.0	35.0	37.0
5	36.01945064761054	37.0	35.0	37.0	35.0	37.0
6	35.62598323831543	37.0	35.0	37.0	35.0	37.0
7	35.97884559808738	37.0	35.0	37.0	35.0	37.0
8	35.95188503270893	37.0	35.0	37.0	35.0	37.0
9	37.77239970575099	39.0	38.0	39.0	35.0	39.0
10	37.46908152273862	39.0	37.0	39.0	35.0	39.0
11	37.45874996715971	39.0	37.0	39.0	35.0	39.0
12	37.31191708483304	39.0	37.0	39.0	35.0	39.0
13	37.208654073509706	39.0	37.0	39.0	34.0	39.0
14	38.393657882983476	40.0	38.0	41.0	34.0	41.0
15	38.44700627906366	40.0	38.0	41.0	34.0	41.0
16	38.51988282584137	40.0	38.0	41.0	34.0	41.0
17	38.473747471297585	40.0	38.0	41.0	34.0	41.0
18	38.44196989202112	40.0	38.0	41.0	34.0	41.0
19	38.484248482778554	40.0	38.0	41.0	34.0	41.0
20	38.4250689646113	40.0	38.0	41.0	34.0	41.0
21	38.343057562462235	40.0	37.0	41.0	34.0	41.0
22	38.34345427317868	40.0	37.0	41.0	34.0	41.0
23	38.27479704700102	40.0	37.0	41.0	34.0	41.0
24	38.22322465386333	40.0	37.0	41.0	34.0	41.0
25	38.1076084386412	40.0	37.0	41.0	34.0	41.0
26	38.06100412474056	40.0	37.0	41.0	34.0	41.0
27	38.03457688568952	40.0	37.0	41.0	34.0	41.0
28	37.963854662007726	40.0	37.0	41.0	34.0	41.0
29	37.97759766702572	40.0	37.0	41.0	34.0	41.0
30	37.88991014896356	40.0	37.0	41.0	33.0	41.0
31	37.80023119564932	40.0	36.0	41.0	33.0	41.0
32	37.744023067020464	40.0	36.0	41.0	33.0	41.0
33	37.695546856527336	40.0	36.0	41.0	33.0	41.0
34	37.67210282951948	40.0	36.0	41.0	33.0	41.0
35	37.677474187531196	40.0	36.0	41.0	33.0	41.0
36	37.58454010456349	40.0	36.0	41.0	33.0	41.0
37	37.490212279641646	40.0	36.0	41.0	33.0	41.0
38	37.451950713291126	40.0	36.0	41.0	33.0	41.0
39	37.435107584793634	40.0	36.0	41.0	33.0	41.0
40	37.308609410713814	39.0	35.0	41.0	32.0	41.0
41	37.19741612589654	39.0	35.0	41.0	32.0	41.0
42	37.23505898116281	39.0	35.0	41.0	32.0	41.0
43	37.138545306465595	39.0	35.0	41.0	32.0	41.0
44	37.01625594409269	39.0	35.0	41.0	32.0	41.0
45	37.03987074061425	39.0	35.0	41.0	32.0	41.0
46	36.93292173501826	39.0	35.0	41.0	31.0	41.0
47	36.85778052176654	39.0	35.0	41.0	31.0	41.0
48	36.84936552557602	39.0	35.0	41.0	31.0	41.0
49	36.86378241336731	39.0	35.0	41.0	32.0	41.0
50	36.801580274807556	39.0	35.0	41.0	31.0	41.0
51	35.90705015369256	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	24.0
11	11.0
12	9.0
13	7.0
14	12.0
15	22.0
16	17.0
17	42.0
18	82.0
19	172.0
20	301.0
21	501.0
22	774.0
23	1241.0
24	1756.0
25	2620.0
26	3773.0
27	4872.0
28	5975.0
29	7359.0
30	9279.0
31	12107.0
32	16183.0
33	23087.0
34	42044.0
35	56870.0
36	56462.0
37	89835.0
38	175346.0
39	250354.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.530909281979877	4.335706591703229	54.12881275779628	17.00457136852061
2	19.76525759924336	6.1701652523447965	51.9194493339989	22.145127814412945
3	20.169718624385887	5.9745684785749935	51.33725665344298	22.518456243596145
4	19.807949977668603	4.595276252528702	50.73995743898274	24.856816330819957
5	19.252554974647296	5.744160996243071	48.70399075217403	26.299293276935607
6	21.795313033654732	7.275963534140767	51.588287838583405	19.340435593621102
7	79.62181120773455	2.1652260725639074	12.107295799069963	6.105666920631585
8	79.24900822320889	4.4292357407456056	9.309040275333	7.012715760712503
9	72.95720253264325	6.2928565798807234	12.587552216062845	8.162388671413183
10	34.196595118619136	32.221317289756456	19.193048367180726	14.389039224443687
11	28.06163465832961	24.805060032052122	27.11911830386465	20.01418700575362
12	26.138113128234767	21.3388329874156	31.53508656700733	20.987967317342303
13	23.02091269737015	23.95791188293093	32.78840343640806	20.23277198329086
14	18.889998160943698	26.833933215984025	30.783832067887452	23.492236555184824
15	16.52878123111683	26.00491290754801	35.84570317631295	21.6206026850222
16	18.636077030186797	25.391193547539604	31.709928276804245	24.262801145469354
17	17.742689751201954	25.74534324672254	30.265480913222813	26.246486088852695
18	18.986154533273783	25.02193731445235	32.30276121167538	23.689146940598484
19	21.035782781178572	25.556183170007618	30.28479100438746	23.123243044426346
20	23.43561989333473	26.302445944880855	30.782781178572368	19.479152983212042
21	23.410529910937132	27.361216929826863	29.130257730604527	20.097995428631478
22	20.723143209941412	25.09536820534377	28.928881065601765	25.252607519113052
23	20.200982581509603	26.698237133173947	28.403567769224708	24.697212516091742
24	22.235504295510076	23.657094816488453	28.910490502587816	25.196910385413656
25	18.859916454299448	26.83445866064157	27.468013556472165	26.837611328586817
26	18.186427764495704	26.44707458686914	27.597798386884904	27.768699261750257
27	19.125397367522265	27.366996821059818	27.905314872711028	25.60229093870688
28	16.344350156319788	26.651341197488378	30.46830255103381	26.536006095158026
29	17.891390589286182	24.243753776633476	28.136641883193654	29.728213750886688
30	19.08414996190526	25.358090534114496	27.298689015579434	28.259070488400813
31	19.108714499645323	29.8402648241074	24.728213750886688	26.322806925360588
32	18.716075979297482	27.224864041194863	26.68838504584505	27.370674933662613
33	18.29900428237396	25.915718676930354	27.28620970496283	28.499067335732864
34	17.53303733284292	25.452801933636337	29.068255261014635	27.945905472506112
35	16.756036045503507	22.792869715997163	28.55817985970628	31.89291437879305
36	17.89848409216299	25.133594304179912	28.077397998055854	28.890523605601242
37	16.834195938312796	27.67254288942017	29.796915639860234	25.6963455324068
38	16.74723484748969	27.58912855003547	28.167117673331056	27.496518929143786
39	18.598245014843812	24.84880329979245	29.312324304442633	27.240627380921108
40	20.047684102671887	24.066810288206394	30.040327877466304	25.84517773165541
41	16.334498068990882	25.634211701652525	29.66844442109135	28.36284580826525
42	18.383994955731286	22.493103538869768	31.30178913905893	27.821112366340017
43	20.019047368835878	22.45146204975961	29.88138086856002	27.648109712844494
44	18.597325486693116	23.38189317710112	27.958516144287103	30.062265191918662
45	18.885925964847754	22.183879357906626	26.110921367206995	32.819273310038625
46	19.722827943146886	23.798964874024644	28.716470062790638	27.761737120037832
47	15.415889446444053	24.092294354097156	33.06097785250768	27.43083834695111
48	16.35446496597746	22.47379344770512	31.988019861808052	29.183721724509365
49	18.02879436723327	20.94921577384862	33.76389144313375	27.25809841578436
50	18.80605837690145	21.116832619604338	30.493392533431418	29.583716470062793
51	16.73672595433886	20.705278091585004	28.21309408086593	34.344901873210205
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134.0
1	453.5
2	773.0
3	1827.0
4	2881.0
5	1958.0
6	1035.0
7	1112.0
8	1189.0
9	1207.5
10	1226.0
11	1236.5
12	1247.0
13	1279.0
14	1311.0
15	1293.5
16	1276.0
17	1214.5
18	1153.0
19	1332.5
20	1512.0
21	1602.5
22	1693.0
23	2075.5
24	2458.0
25	3063.5
26	5069.5
27	6470.0
28	7642.0
29	8814.0
30	10406.0
31	11998.0
32	13161.0
33	14324.0
34	16637.5
35	18951.0
36	19724.5
37	20498.0
38	23375.0
39	26252.0
40	30103.5
41	33955.0
42	38600.0
43	43245.0
44	49293.5
45	55342.0
46	70166.5
47	84991.0
48	95089.0
49	105187.0
50	99604.5
51	94022.0
52	76523.0
53	59024.0
54	49726.0
55	40428.0
56	35693.0
57	30958.0
58	27699.0
59	24440.0
60	22268.0
61	20096.0
62	17514.0
63	14932.0
64	12230.0
65	9528.0
66	7782.5
67	6037.0
68	4813.0
69	3589.0
70	2935.5
71	2282.0
72	1946.5
73	1611.0
74	1497.0
75	1042.0
76	701.0
77	527.5
78	354.0
79	287.0
80	220.0
81	137.0
82	54.0
83	30.5
84	7.0
85	5.5
86	4.0
87	3.0
88	2.0
89	2.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	761260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.190819525732742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.0297040745505	19.274611749777435
2	13.828523436360376	8.073318638763226
3	5.942009042077814	5.203563407026967
4	3.173794405261856	3.7058263878311655
5	1.8278002685266803	2.6677493883824086
6	1.1977526920240278	2.097802960160037
7	0.8262136927170418	1.6882498355654665
8	0.6055923617356976	1.4142189870071207
9	0.44509852920775855	1.169351175354549
>10	5.745345557666867	39.433444615924394
>50	0.28544256044270694	5.1544842471911165
>100	0.07999664107576045	4.158190331574552
>500	0.006817895546229584	1.1548464788138129
>1k	0.004999790067235028	3.3104979886125285
>5k	9.090527394972779E-4	1.4938438080152483
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	5999	0.7880356251477812	Illumina PCR Primer Index 11 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT	5260	0.6909597246669994	Illumina PCR Primer Index 11 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	4995	0.6561490161048787	RNA PCR Primer, Index 14 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	4804	0.6310590337072748	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	2353	0.3090928197987547	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	2305	0.3027874839082574	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	2094	0.27507027822294616	No Hit
TCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	1856	0.2438063210992302	Illumina PCR Primer Index 11 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1684	0.22121220082494808	No Hit
CGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	1388	0.18232929616688123	Illumina PCR Primer Index 11 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	1264	0.16604051178309645	RNA PCR Primer, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	1189	0.15618842445419437	RNA PCR Primer, Index 14 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	1019	0.13385702650868297	RNA PCR Primer, Index 14 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	791	0.10390668102882065	RNA PCR Primer, Index 14 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	788	0.10351259753566455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3136116438536112E-4	0.0	0.0	0.7294485458319102	0.0
2	1.3136116438536112E-4	0.0	0.0	2.97927120825999	0.0
3	1.3136116438536112E-4	0.0	0.0	4.226676825263379	0.0
4	1.3136116438536112E-4	0.0	0.0	5.490371226650553	0.0
5	1.3136116438536112E-4	0.0	0.0	7.744660168667735	0.0
6	1.3136116438536112E-4	0.0	0.0	8.855056091217193	0.0
7	1.3136116438536112E-4	0.0	0.0	9.959672122533695	0.0
8	1.3136116438536112E-4	0.0	0.0	11.678532958516143	0.0
9	1.3136116438536112E-4	0.0	0.0	12.392743609279353	0.0
10	1.3136116438536112E-4	0.0	0.0	14.071407928959882	0.0
11	1.3136116438536112E-4	0.0	0.0	16.62375535296745	0.0
12	1.3136116438536112E-4	0.0	0.0	19.418201402937235	0.0
13	1.3136116438536112E-4	0.0	0.0	20.252082074455508	0.0
14	1.3136116438536112E-4	0.0	0.0	20.54396658171978	0.0
15	1.3136116438536112E-4	0.0	0.0	21.051940204397972	0.0
16	1.3136116438536112E-4	0.0	0.0	22.062895725507712	0.0
17	1.3136116438536112E-4	0.0	0.0	23.460972598061108	0.0
18	1.3136116438536112E-4	0.0	0.0	24.973070961301	0.0
19	1.3136116438536112E-4	0.0	0.0	26.007671492000107	0.0
20	1.3136116438536112E-4	0.0	0.0	27.147755037700655	0.0
21	1.3136116438536112E-4	0.0	0.0	28.592465123610857	0.0
22	1.3136116438536112E-4	0.0	0.0	30.06423560938444	0.0
23	1.3136116438536112E-4	0.0	0.0	31.38927567453958	0.0
24	1.3136116438536112E-4	0.0	0.0	32.41231642277277	0.0
25	1.3136116438536112E-4	0.0	0.0	33.32356882011402	0.0
26	1.3136116438536112E-4	0.0	0.0	34.14536426450884	0.0
27	1.3136116438536112E-4	0.0	0.0	34.97569818458871	0.0
28	1.3136116438536112E-4	0.0	0.0	35.78291253973675	0.0
29	1.3136116438536112E-4	0.0	0.0	36.61167012584399	0.0
30	1.3136116438536112E-4	0.0	0.0	37.598061109213674	0.0
31	1.3136116438536112E-4	0.0	0.0	38.47581640963666	0.0
32	1.3136116438536112E-4	0.0	0.0	39.31179885978509	0.0
33	1.3136116438536112E-4	0.0	0.0	40.12768305178257	0.0
34	1.3136116438536112E-4	0.0	0.0	40.90048078186165	0.0
35	1.3136116438536112E-4	0.0	0.0	41.702046606941124	0.0
36	1.3136116438536112E-4	0.0	0.0	42.473136641883194	0.0
37	1.3136116438536112E-4	0.0	0.0	43.26471901846938	0.0
38	1.3136116438536112E-4	0.0	0.0	44.02503743793185	0.0
39	1.3136116438536112E-4	0.0	0.0	44.76552032157213	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGCG	65	0.0	45.000004	1
GTCAACG	35	1.2112105E-7	45.000004	1
CGAGTTG	20	7.031784E-4	45.0	6
GATCGAG	25	3.88956E-5	45.0	11
TACGCGG	25	3.88956E-5	45.0	2
TGATTCG	20	7.031784E-4	45.0	1
CGCACTT	80	0.0	45.0	35
GGCGATA	25	3.88956E-5	45.0	8
CGATATT	25	3.88956E-5	45.0	12
CGAGACT	30	2.164532E-6	44.999996	26
TACGTTG	30	2.164532E-6	44.999996	1
TTTACGT	80	0.0	42.1875	26
CGTCCGG	60	3.6379788E-12	41.249996	2
CGGTAGT	55	6.002665E-11	40.909092	12
TGGGACG	220	0.0	40.909092	6
CGCTAGA	50	1.0804797E-9	40.5	24
GCCAACG	45	1.9268555E-8	40.000004	9
TTACACG	130	0.0	39.807693	34
CGTTTGG	425	0.0	39.70588	2
TCTTGCG	250	0.0	39.600002	1
>>END_MODULE