SRR2933972.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933972.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262543
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	3592	1.3681568352612714	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	3455	1.3159749069676205	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2847	1.084393794540323	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2280	0.8684291716023661	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	1204	0.4585915450040565	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	1179	0.44906929531543405	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	999	0.3805090975573525	No Hit
TCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	914	0.34813344861603623	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	676	0.25748163158035065	No Hit
TTCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	602	0.22929577250202826	No Hit
CGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	570	0.21710729290059153	No Hit
TTCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	511	0.19463478363544257	No Hit
GCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	491	0.18701698388454463	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.18054185409628137	No Hit
TGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	444	0.16911515446993444	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	383	0.1458808652296957	No Hit
TTTCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	344	0.1310261557154447	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	322	0.12264657598945697	No Hit
TGCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	294	0.11198165633819984	No Hit
GTCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	277	0.10550652654993657	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTC	271	0.1032211866246672	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	45	3.8198777E-10	45.000004	1
CGTTCTG	45	3.8198777E-10	45.000004	1
GCTACGA	30	2.1590804E-6	45.000004	10
ATCCTTC	20	7.0233323E-4	45.0	26
GTTTGCG	40	6.7811925E-9	45.0	1
CTTCGCG	20	7.0233323E-4	45.0	1
TACCCTA	20	7.0233323E-4	45.0	9
GTATTGC	35	1.207136E-7	45.0	11
CGCGGGC	50	2.1827873E-11	45.0	4
ACGTTAC	20	7.0233323E-4	45.0	38
GCGCGAC	20	7.0233323E-4	45.0	9
CTTCACG	25	3.8825572E-5	45.0	1
AGTGGCA	20	7.0233323E-4	45.0	19
CGCGACC	20	7.0233323E-4	45.0	10
CGTTCGG	25	3.8825572E-5	45.0	2
GCTTGCG	25	3.8825572E-5	45.0	1
AGTATCC	25	3.8825572E-5	45.0	23
CGTTACC	20	7.0233323E-4	45.0	39
ATCCGGT	25	3.8825572E-5	45.0	12
ACTAGAC	25	3.8825572E-5	45.0	22

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content