##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933972.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262543
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40039917270695	33.0	31.0	34.0	31.0	34.0
2	32.626765901204756	34.0	31.0	34.0	31.0	34.0
3	32.698575090556595	34.0	31.0	34.0	31.0	34.0
4	36.07259763162606	37.0	35.0	37.0	35.0	37.0
5	36.110964680071454	37.0	35.0	37.0	35.0	37.0
6	35.72146657880804	37.0	35.0	37.0	35.0	37.0
7	36.04508594782569	37.0	35.0	37.0	35.0	37.0
8	36.01709053374114	37.0	35.0	37.0	35.0	37.0
9	37.839028273463775	39.0	38.0	39.0	35.0	39.0
10	37.639053412202955	39.0	37.0	39.0	35.0	39.0
11	37.62664401640874	39.0	37.0	39.0	35.0	39.0
12	37.408546409540534	39.0	37.0	39.0	35.0	39.0
13	37.25014949932011	39.0	37.0	39.0	34.0	39.0
14	38.38530069360067	40.0	38.0	41.0	34.0	41.0
15	38.44786568295479	40.0	38.0	41.0	34.0	41.0
16	38.53848702879147	40.0	38.0	41.0	35.0	41.0
17	38.542673009754594	40.0	38.0	41.0	35.0	41.0
18	38.52963514548093	40.0	38.0	41.0	35.0	41.0
19	38.572732847571636	40.0	38.0	41.0	35.0	41.0
20	38.507829193693986	40.0	38.0	41.0	35.0	41.0
21	38.42629969186	40.0	37.0	41.0	34.0	41.0
22	38.43173499198227	40.0	37.0	41.0	34.0	41.0
23	38.366789440205984	40.0	37.0	41.0	34.0	41.0
24	38.34419123724495	40.0	37.0	41.0	34.0	41.0
25	38.26170189264235	40.0	37.0	41.0	34.0	41.0
26	38.18263674902778	40.0	37.0	41.0	34.0	41.0
27	38.11459456165276	40.0	37.0	41.0	34.0	41.0
28	38.085658349298974	40.0	37.0	41.0	34.0	41.0
29	38.02232777106988	40.0	36.0	41.0	34.0	41.0
30	37.97239690260262	40.0	36.0	41.0	34.0	41.0
31	37.81203840894634	40.0	36.0	41.0	34.0	41.0
32	37.4844311217591	40.0	35.0	41.0	33.0	41.0
33	37.50258433856549	39.0	36.0	41.0	33.0	41.0
34	37.477685560079685	39.0	35.0	41.0	33.0	41.0
35	37.36187595936666	39.0	35.0	41.0	33.0	41.0
36	37.35159192970294	39.0	35.0	41.0	33.0	41.0
37	37.2525871952404	39.0	35.0	41.0	33.0	41.0
38	37.19895788499408	39.0	35.0	41.0	33.0	41.0
39	37.25488015296542	39.0	35.0	41.0	33.0	41.0
40	37.121835280315985	39.0	35.0	41.0	32.0	41.0
41	37.00481444944257	39.0	35.0	41.0	32.0	41.0
42	36.92496848135353	39.0	35.0	41.0	32.0	41.0
43	36.89254712561371	39.0	35.0	41.0	32.0	41.0
44	36.82790628582747	39.0	35.0	41.0	32.0	41.0
45	36.89118734835817	39.0	35.0	41.0	32.0	41.0
46	36.831075290523835	39.0	35.0	41.0	32.0	41.0
47	36.689898416640325	39.0	35.0	40.0	31.0	41.0
48	36.61964706733754	39.0	35.0	40.0	31.0	41.0
49	36.71563515309873	39.0	35.0	40.0	32.0	41.0
50	36.599928392682344	39.0	35.0	40.0	31.0	41.0
51	35.61452028810519	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	15.0
10	22.0
11	25.0
12	12.0
13	9.0
14	7.0
15	10.0
16	10.0
17	15.0
18	37.0
19	57.0
20	130.0
21	226.0
22	358.0
23	521.0
24	754.0
25	967.0
26	1311.0
27	1485.0
28	1787.0
29	2142.0
30	2767.0
31	3662.0
32	5080.0
33	7685.0
34	15663.0
35	22351.0
36	19547.0
37	31314.0
38	63466.0
39	81055.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.40324061201403	4.335289838236022	53.075115314443735	18.186354235306215
2	18.335282220436273	6.77793732836145	51.82236814540856	23.06441230579372
3	20.859440167896306	6.5086481071672075	50.23139066743353	22.40052105750296
4	19.752192974103288	3.633309591190776	52.05394925783586	24.560548176870075
5	18.935183950819486	5.826855029461841	50.06798886277677	25.16997215694191
6	20.843061898431873	7.208343014287183	52.185356303538846	19.763238783742093
7	76.77332855951215	2.039284993315381	14.611701702197355	6.575684744975109
8	75.5750486586959	4.77064709399984	10.956681381716518	8.697622865587732
9	68.40289019322549	6.587873224576546	15.319776189043319	9.689460393154645
10	32.4072628102825	30.78238612341597	21.74539027892574	15.064960787375783
11	27.475118361563627	25.62551658204561	27.95427796589511	18.94508709049565
12	25.866619944161528	20.868962417584928	34.521583131144226	18.74283450710931
13	24.83555074787749	26.221990302540917	30.994160956490934	17.948297993090655
14	17.44552320953139	29.72275017806607	30.92826698864567	21.90345962375687
15	14.103975348800008	26.302358089912893	39.358124192989344	20.235542368297764
16	16.770967041589376	27.067566074890586	30.251044590790844	25.91042229272919
17	17.234129266443972	25.044659351039638	28.288318484971985	29.432892897544406
18	18.895571392114814	23.56452085944017	32.7287339597704	24.81117378867462
19	20.68613522356338	24.281355815999664	31.772700091032707	23.25980886940425
20	23.638413517023878	24.141188300583142	32.777487878176146	19.442910304216834
21	22.414613987042124	26.931969239324605	30.811714652456928	19.84170212117634
22	19.54536971086641	24.328586174455232	29.379187409300574	26.746856705377787
23	18.939754630670024	25.682650080177343	29.541065654007152	25.83652963514548
24	22.4313731464941	22.348720019196854	28.969349782702263	26.250557051606783
25	17.69272081144803	26.37015650769588	29.520878484667275	26.416244196188813
26	17.17394864841188	27.849533219320264	30.85589789101214	24.12062024125572
27	19.447480984067372	30.686782736542206	28.113109090701332	21.752627188689093
28	17.69424437139821	27.228301649634535	32.670838681663575	22.40661529730368
29	18.623235051020217	28.38849255169629	29.96461532015708	23.023657077126416
30	24.426474901254274	28.186239968309952	26.6824862974827	20.704798832953077
31	27.510160240417758	28.26622686569438	24.19337022887679	20.030242665011063
32	24.55064503719391	28.835657397074005	26.33168661895385	20.282010946778243
33	28.025123503578463	25.009236582197964	25.703979919479856	21.261659994743717
34	23.793816631942196	23.064793195781263	30.29522782934605	22.846162342930494
35	20.833920538730798	23.83761898050986	29.85644256369433	25.472017917065017
36	27.60347828736626	24.07034276289979	30.04688755746678	18.279291392267172
37	24.425332231291634	27.734885333069247	29.076760759189924	18.763021676449192
38	23.818574481132615	28.75376604975185	26.2795046906602	21.14815477845534
39	27.188308200942323	25.093032379457842	28.017124813840017	19.701534605759818
40	28.43991270001485	22.689997448037083	28.004936334238582	20.86515351770948
41	22.66562048883421	22.605820760789662	27.88190886826158	26.846649882114548
42	24.055868943373085	22.390998807814338	29.59400936227589	23.959122886536683
43	26.81465512316078	23.72639910414675	26.329782169016124	23.12916360367635
44	23.458633442902688	23.378265655530715	28.019029263777746	25.144071637788855
45	23.060984295905815	24.08024590257596	26.0589693878717	26.799800413646523
46	26.658490228267368	22.952049759467975	29.825971364690734	20.563488647573923
47	21.07769013075953	22.781791935035404	34.63623101739525	21.504286916809818
48	21.871845754790645	21.366023851331022	32.81367242699291	23.948457966885424
49	24.274499796223857	18.89366694217709	34.85943255009655	21.972400711502495
50	24.37200763303535	20.127369611835014	30.219430721824615	25.28119203330502
51	21.669974061391848	19.47604773313324	27.96532377553391	30.888654429941
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	133.0
2	193.0
3	551.0
4	909.0
5	647.5
6	386.0
7	447.5
8	509.0
9	578.5
10	648.0
11	647.0
12	646.0
13	680.0
14	714.0
15	667.5
16	621.0
17	601.5
18	582.0
19	637.5
20	693.0
21	830.0
22	967.0
23	1081.5
24	1196.0
25	1224.0
26	1625.0
27	1998.0
28	2124.5
29	2251.0
30	2658.5
31	3066.0
32	3624.5
33	4183.0
34	5119.0
35	6055.0
36	6152.5
37	6250.0
38	7146.5
39	8043.0
40	9603.5
41	11164.0
42	14175.0
43	17186.0
44	20044.5
45	22903.0
46	28612.5
47	34322.0
48	36686.5
49	39051.0
50	35807.0
51	32563.0
52	24976.0
53	17389.0
54	14745.0
55	12101.0
56	10510.5
57	8920.0
58	8349.5
59	7779.0
60	6849.5
61	5920.0
62	5176.5
63	4433.0
64	3498.5
65	2564.0
66	2190.5
67	1817.0
68	1570.5
69	1324.0
70	1042.0
71	760.0
72	608.0
73	456.0
74	388.0
75	263.0
76	206.0
77	129.5
78	53.0
79	47.5
80	42.0
81	29.5
82	17.0
83	12.5
84	8.0
85	4.5
86	1.0
87	4.5
88	8.0
89	4.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	262543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.779990325394316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.66115665204596	24.544931687380732
2	11.837135093080159	7.997166178492667
3	4.444845355012572	4.5044049927059575
4	2.1333453606503774	2.8825754257397835
5	1.23467926528127	2.0853726818083134
6	0.818609266296075	1.659156785745573
7	0.5897143888055747	1.3944382444018693
8	0.46004487692671975	1.2432249193465452
9	0.3833707307722665	1.1655233618873861
>10	5.243158523797174	38.436751313118236
>50	0.11275609728596074	2.63652049378578
>100	0.06765365837157644	4.278156340104287
>500	0.006765365837157644	1.6271620267918019
>1k	0.006765365837157644	5.5446155486910715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	3592	1.3681568352612714	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	3455	1.3159749069676205	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2847	1.084393794540323	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2280	0.8684291716023661	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	1204	0.4585915450040565	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	1179	0.44906929531543405	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	999	0.3805090975573525	No Hit
TCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	914	0.34813344861603623	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	676	0.25748163158035065	No Hit
TTCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	602	0.22929577250202826	No Hit
CGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	570	0.21710729290059153	No Hit
TTCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	511	0.19463478363544257	No Hit
GCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	491	0.18701698388454463	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.18054185409628137	No Hit
TGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	444	0.16911515446993444	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	383	0.1458808652296957	No Hit
TTTCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	344	0.1310261557154447	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT	322	0.12264657598945697	No Hit
TGCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	294	0.11198165633819984	No Hit
GTCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	277	0.10550652654993657	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTC	271	0.1032211866246672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4165298636794734	0.0
2	0.0	0.0	0.0	5.090594683537554	0.0
3	0.0	0.0	0.0	6.809932087315221	0.0
4	0.0	0.0	0.0	8.772277303146533	0.0
5	0.0	0.0	0.0	11.653329168936136	0.0
6	0.0	0.0	0.0	12.9213119374731	0.0
7	0.0	0.0	0.0	14.153871937168388	0.0
8	0.0	0.0	0.0	15.794746003511806	0.0
9	0.0	0.0	0.0	16.618230156583873	0.0
10	0.0	0.0	0.0	18.576004692564645	0.0
11	0.0	0.0	0.0	21.081879920622526	0.0
12	0.0	0.0	0.0	24.158709240010207	0.0
13	0.0	0.0	0.0	25.07893944991868	0.0
14	0.0	0.0	0.0	25.42554933858454	0.0
15	0.0	0.0	0.0	25.991551860076253	0.0
16	0.0	0.0	0.0	27.30105163725561	0.0
17	0.0	0.0	0.0	29.342241080508717	0.0
18	0.0	0.0	0.0	32.02827727267533	0.0
19	0.0	0.0	0.0	33.309591190776366	0.0
20	0.0	0.0	0.0	34.832770250968416	0.0
21	0.0	0.0	0.0	36.11141793915663	0.0
22	7.617799750897948E-4	0.0	0.0	37.128775095889054	0.0
23	0.0011426699626346922	0.0	0.0	38.105757913941716	0.0
24	0.0011426699626346922	0.0	0.0	38.81802219065067	0.0
25	0.0011426699626346922	0.0	0.0	39.47010584932754	0.0
26	0.0011426699626346922	0.0	0.0	40.05705732013423	0.0
27	0.0011426699626346922	0.0	0.0	40.65086481071672	0.0
28	0.0011426699626346922	0.0	0.0	41.25457544097538	0.0
29	0.0011426699626346922	0.0	0.0	41.962649927821346	0.0
30	0.0011426699626346922	0.0	0.0	42.70233828363354	0.0
31	0.0011426699626346922	0.0	0.0	43.35746906221076	0.0
32	0.0011426699626346922	0.0	0.0	43.922709803727386	0.0
33	0.0015235599501795896	0.0	0.0	44.44186285675109	0.0
34	0.0015235599501795896	0.0	0.0	45.03681301729621	0.0
35	0.0015235599501795896	0.0	0.0	45.60548176870075	0.0
36	0.0015235599501795896	0.0	0.0	46.17415052010528	0.0
37	0.0015235599501795896	0.0	0.0	46.69330357312897	0.0
38	0.0015235599501795896	0.0	0.0	47.21283751614021	0.0
39	0.0015235599501795896	0.0	0.0	47.83330730585085	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	45	3.8198777E-10	45.000004	1
CGTTCTG	45	3.8198777E-10	45.000004	1
GCTACGA	30	2.1590804E-6	45.000004	10
ATCCTTC	20	7.0233323E-4	45.0	26
GTTTGCG	40	6.7811925E-9	45.0	1
CTTCGCG	20	7.0233323E-4	45.0	1
TACCCTA	20	7.0233323E-4	45.0	9
GTATTGC	35	1.207136E-7	45.0	11
CGCGGGC	50	2.1827873E-11	45.0	4
ACGTTAC	20	7.0233323E-4	45.0	38
GCGCGAC	20	7.0233323E-4	45.0	9
CTTCACG	25	3.8825572E-5	45.0	1
AGTGGCA	20	7.0233323E-4	45.0	19
CGCGACC	20	7.0233323E-4	45.0	10
CGTTCGG	25	3.8825572E-5	45.0	2
GCTTGCG	25	3.8825572E-5	45.0	1
AGTATCC	25	3.8825572E-5	45.0	23
CGTTACC	20	7.0233323E-4	45.0	39
ATCCGGT	25	3.8825572E-5	45.0	12
ACTAGAC	25	3.8825572E-5	45.0	22
>>END_MODULE