##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933971.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	919362
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39394928221962	33.0	31.0	34.0	31.0	34.0
2	32.6154811706379	34.0	31.0	34.0	31.0	34.0
3	32.67472334075152	34.0	31.0	34.0	31.0	34.0
4	36.06584022398141	37.0	35.0	37.0	35.0	37.0
5	36.102872426748114	37.0	35.0	37.0	35.0	37.0
6	35.76684483369989	37.0	35.0	37.0	35.0	37.0
7	36.07790293703677	37.0	35.0	37.0	35.0	37.0
8	36.07897324448911	37.0	36.0	37.0	35.0	37.0
9	37.93001777319489	39.0	38.0	39.0	35.0	39.0
10	37.570508678844675	39.0	37.0	39.0	35.0	39.0
11	37.49815741786152	39.0	37.0	39.0	35.0	39.0
12	37.35023418414074	39.0	37.0	39.0	35.0	39.0
13	37.301782105416585	39.0	37.0	39.0	34.0	39.0
14	38.52681315956065	40.0	38.0	41.0	35.0	41.0
15	38.56870960513922	40.0	38.0	41.0	35.0	41.0
16	38.61681579182085	40.0	38.0	41.0	35.0	41.0
17	38.57089046534445	40.0	38.0	41.0	35.0	41.0
18	38.49810738316354	40.0	38.0	41.0	34.0	41.0
19	38.55413101694436	40.0	38.0	41.0	34.0	41.0
20	38.51387483929073	40.0	38.0	41.0	34.0	41.0
21	38.436019761530275	40.0	38.0	41.0	34.0	41.0
22	38.43379974373533	40.0	38.0	41.0	34.0	41.0
23	38.3935990393338	40.0	37.0	41.0	34.0	41.0
24	38.37166970137987	40.0	37.0	41.0	34.0	41.0
25	38.28250351874452	40.0	37.0	41.0	34.0	41.0
26	38.233109482445435	40.0	37.0	41.0	34.0	41.0
27	38.19546489848395	40.0	37.0	41.0	34.0	41.0
28	38.153739223505	40.0	37.0	41.0	34.0	41.0
29	38.14538342894311	40.0	37.0	41.0	34.0	41.0
30	38.04012565235457	40.0	37.0	41.0	34.0	41.0
31	38.00536132665914	40.0	37.0	41.0	34.0	41.0
32	37.94108849397735	40.0	37.0	41.0	34.0	41.0
33	37.860397754094684	40.0	37.0	41.0	33.0	41.0
34	37.85467748286312	40.0	37.0	41.0	33.0	41.0
35	37.791296573058275	40.0	37.0	41.0	33.0	41.0
36	37.689330209427844	40.0	36.0	41.0	33.0	41.0
37	37.62743293718905	40.0	36.0	41.0	33.0	41.0
38	37.626637820575574	40.0	36.0	41.0	33.0	41.0
39	37.59331253630235	40.0	36.0	41.0	33.0	41.0
40	37.504702173898856	40.0	36.0	41.0	33.0	41.0
41	37.422566954039866	40.0	36.0	41.0	33.0	41.0
42	37.39288985187554	40.0	36.0	41.0	33.0	41.0
43	37.25437749221743	39.0	35.0	41.0	32.0	41.0
44	37.1180481681862	39.0	35.0	41.0	32.0	41.0
45	37.13013481087972	39.0	35.0	41.0	32.0	41.0
46	37.060337494914954	39.0	35.0	41.0	32.0	41.0
47	36.98774693755017	39.0	35.0	41.0	32.0	41.0
48	36.948707908310325	39.0	35.0	41.0	32.0	41.0
49	36.92239944657273	39.0	35.0	41.0	32.0	41.0
50	36.82776207848486	39.0	35.0	41.0	31.0	41.0
51	35.87843199958232	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	11.0
11	21.0
12	8.0
13	12.0
14	9.0
15	15.0
16	16.0
17	34.0
18	91.0
19	184.0
20	350.0
21	566.0
22	894.0
23	1354.0
24	1980.0
25	2992.0
26	4288.0
27	5556.0
28	6652.0
29	8334.0
30	10762.0
31	13741.0
32	18516.0
33	26184.0
34	50192.0
35	67496.0
36	62909.0
37	100817.0
38	201390.0
39	333740.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.0378240562477	4.249359882179164	56.54203676027506	16.170779301298076
2	18.491627889775735	4.424481325092836	55.314881406888695	21.76900937824274
3	20.096001357463113	4.3124471100611075	54.31755935094119	21.273992181534588
4	19.51385852362834	4.511389420054343	51.961251389550576	24.013500666766735
5	18.445508950772382	5.291821937387014	51.63352411781214	24.62914499402847
6	21.473695889105706	5.742460532412695	53.35243353543	19.431410043051596
7	82.65786491066632	2.1877127834302486	9.861947742021098	5.292474563882345
8	83.94245139564175	2.7690942196871307	7.568291924182205	5.720162460488904
9	78.35520719803516	5.54308313808924	10.189783784842096	5.911925879033504
10	35.03766742588883	36.703605326302366	15.941816172519637	12.316911075289168
11	26.499463758563003	24.471862008653826	29.967956039079276	19.06071819370389
12	25.600361990162746	21.483485286535664	32.55964462311908	20.35650810018252
13	23.82804597101033	22.46927760773232	33.82845930112404	19.874217120133313
14	19.13729303582267	25.46037360691436	31.828594177266407	23.573739179996565
15	17.053347865149963	26.452257108734102	35.34102997513493	21.15336505098101
16	20.253175571755193	25.404247728315944	32.09160265488458	22.25097404504428
17	20.432321544723404	26.49435151768291	29.427254987697992	23.64607194989569
18	19.66080825616025	26.153245402790198	32.234201544114285	21.951744796935266
19	21.61052991095999	26.838068138556952	29.948050930971696	21.603351019511358
20	23.672720865121683	27.488519212236312	30.45960133222822	18.379158590413788
21	24.643067692595515	26.89593435447626	29.776083849452117	18.684914103476107
22	22.10609096308092	25.02169983096974	29.82992553531688	23.04228367063246
23	20.444068821639355	25.265455826975664	30.55923564384867	23.731239707536314
24	22.911432058318702	23.26733104043891	30.696722292198288	23.1245146090441
25	21.152277340155457	25.3605217531288	28.01866946860975	25.46853143810599
26	20.09698029720611	27.794709809628852	28.14734565927241	23.960964233892636
27	19.690937846028007	27.85083568822727	29.498935131101785	22.959291334642938
28	17.01342887785225	26.453997446054984	30.96919385399875	25.563379822094017
29	19.460778235341465	25.229887682980152	28.878722418372742	26.43061166330564
30	20.364557160291593	27.47775087506336	29.20829879851462	22.949393166130424
31	20.02181947916055	27.386600707882202	28.76222858895626	23.82935122400099
32	20.887637296298955	27.21920201182994	28.69566068643255	23.197500005438553
33	22.186581564171675	26.98795469031785	27.891624844185426	22.93383890132505
34	19.47143780143186	24.476104080873476	31.529038615909727	24.523419501784932
35	19.802972061059734	24.773484220579054	30.28904827478186	25.134495443579354
36	21.733985089660006	27.466547453560185	28.38435784815992	22.41510960861989
37	19.022322001561953	28.804214226822516	29.83525531836208	22.338208453253454
38	19.261944696430785	27.248461433037257	28.249372934709072	25.240220935822887
39	20.676947709389772	25.8822966361455	29.56582934687316	23.87492630759157
40	21.982635784380907	24.36994350429972	31.46714786993589	22.18027284138348
41	19.53202329441504	24.66003598147411	30.22911540829401	25.57882531581684
42	21.224718881137136	24.29282480676817	29.101594366528094	25.3808619455666
43	22.652448110755067	23.54643763827524	28.320509222700093	25.480605028269604
44	20.321701353764894	23.701980286328997	28.13407558720069	27.842242772705422
45	20.57720462668677	23.183577306871506	27.81461491773643	28.424603148705295
46	20.91689671750627	24.466314683443517	30.162329963605195	24.454458635445015
47	17.757640624694083	24.658513186318338	32.83048461868122	24.753361570306364
48	18.066006643737722	22.70204772440018	32.384958264535626	26.846987367326474
49	20.120692393203115	21.471302925289496	33.11459468631507	25.293409995192317
50	20.422205834045783	21.515790298054522	30.799619736295387	27.262384131604307
51	18.13061666677544	21.361009047578648	29.694396766453256	30.813977519192658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117.0
1	560.5
2	1004.0
3	1919.0
4	2834.0
5	1980.5
6	1127.0
7	1181.5
8	1236.0
9	1248.5
10	1261.0
11	1332.5
12	1404.0
13	1426.0
14	1448.0
15	1491.0
16	1534.0
17	1579.5
18	1625.0
19	1961.5
20	2298.0
21	2936.5
22	3575.0
23	4244.5
24	4914.0
25	6024.0
26	8769.0
27	10404.0
28	12611.5
29	14819.0
30	16573.0
31	18327.0
32	19734.0
33	21141.0
34	24341.5
35	27542.0
36	29869.0
37	32196.0
38	35634.5
39	39073.0
40	43528.0
41	47983.0
42	51356.5
43	54730.0
44	61312.0
45	67894.0
46	78027.0
47	88160.0
48	100975.0
49	113790.0
50	108691.5
51	103593.0
52	84273.0
53	64953.0
54	55710.5
55	46468.0
56	41742.0
57	37016.0
58	33574.0
59	30132.0
60	28252.5
61	26373.0
62	21864.5
63	17356.0
64	13873.5
65	10391.0
66	8634.5
67	6878.0
68	5066.0
69	3254.0
70	2745.0
71	2236.0
72	1834.5
73	1433.0
74	1125.0
75	649.5
76	482.0
77	358.5
78	235.0
79	177.0
80	119.0
81	79.5
82	40.0
83	27.0
84	14.0
85	7.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	919362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.24927401221995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.00700043566573	18.646498420318423
2	13.86864915573714	7.835585403595226
3	6.011546136819232	5.094654421683263
4	3.0906389823811056	3.4923322994452985
5	1.7807454917660128	2.5152383671461727
6	1.1141050213604922	1.8883594816081621
7	0.7354911639084927	1.4543963995972309
8	0.5630795083610638	1.2725269857886226
9	0.4254007033275217	1.0815534929961221
>10	5.901071907889839	41.37943719336474
>50	0.4131085755800799	7.061845289083829
>100	0.08059704742829486	4.105403936624427
>500	0.004672292604538794	0.8115115939561924
>1k	0.002336146302269397	0.9529596706473912
>5k	0.001557430868179598	2.4076970441448973
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	6674	0.7259382049725788	TruSeq Adapter, Index 27 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	5121	0.5570167137645454	TruSeq Adapter, Index 27 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	5068	0.5512518463891264	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	5027	0.5467922320043682	TruSeq Adapter, Index 27 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG	2030	0.22080529758680476	No Hit
CGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG	1544	0.1679425514650377	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1412	0.15358476856776765	No Hit
GCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG	1283	0.13955329891816282	No Hit
TGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG	1253	0.13629016644151054	No Hit
TTCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	1142	0.12421657627789706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5798586411011114	0.0
2	0.0	0.0	0.0	2.5602537411813846	0.0
3	0.0	0.0	0.0	3.655034687098227	0.0
4	0.0	0.0	0.0	4.679875826932155	0.0
5	0.0	0.0	0.0	6.318294643459269	0.0
6	0.0	0.0	0.0	7.223813905730278	0.0
7	0.0	0.0	0.0	8.074838855641195	0.0
8	0.0	0.0	0.0	9.399453098996913	0.0
9	0.0	0.0	0.0	9.960168029568331	0.0
10	0.0	0.0	0.0	11.168179672425008	0.0
11	0.0	0.0	0.0	13.350345130644948	0.0
12	0.0	0.0	0.0	15.42308687981448	0.0
13	0.0	0.0	0.0	16.10345000119648	0.0
14	0.0	0.0	0.0	16.346118286376857	0.0
15	0.0	0.0	0.0	16.72094343686165	0.0
16	0.0	0.0	0.0	17.585891085339615	0.0
17	0.0	0.0	0.0	18.848614582721495	0.0
18	0.0	0.0	0.0	20.192263765524352	0.0
19	0.0	0.0	0.0	21.21046986932242	0.0
20	0.0	0.0	0.0	22.253802093190714	0.0
21	0.0	0.0	0.0	23.61115643239551	0.0
22	0.0	0.0	0.0	25.09163963705265	0.0
23	0.0	0.0	0.0	26.354471905517087	0.0
24	0.0	0.0	0.0	27.343853672438062	0.0
25	0.0	0.0	0.0	28.198468068073293	0.0
26	0.0	0.0	0.0	28.99075663340447	0.0
27	0.0	0.0	0.0	29.73518592241141	0.0
28	0.0	0.0	0.0	30.56054089683933	0.0
29	0.0	0.0	0.0	31.396337895192534	0.0
30	0.0	0.0	0.0	32.32959378351509	0.0
31	1.0877108255507624E-4	0.0	0.0	33.205200998083455	0.0
32	1.0877108255507624E-4	0.0	0.0	33.985633515416126	0.0
33	1.0877108255507624E-4	0.0	0.0	34.77085196038122	0.0
34	1.0877108255507624E-4	0.0	0.0	35.49037267148305	0.0
35	1.0877108255507624E-4	0.0	0.0	36.293538345069734	0.0
36	1.0877108255507624E-4	0.0	0.0	37.05928676625747	0.0
37	1.0877108255507624E-4	0.0	0.0	37.82275099471155	0.0
38	1.0877108255507624E-4	0.0	0.0	38.61145011431841	0.0
39	1.0877108255507624E-4	0.0	0.0	39.42614552265593	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAT	30	2.1650249E-6	45.000004	12
AGCGTCA	25	3.890195E-5	45.0	35
ACGCCCG	20	7.03255E-4	45.0	20
GGTCGAC	20	7.03255E-4	45.0	9
TAGCGAT	20	7.03255E-4	45.0	14
TATTGCG	75	0.0	45.0	1
ACGGAAC	20	7.03255E-4	45.0	10
GGAACGT	20	7.03255E-4	45.0	8
GGACGTA	25	3.890195E-5	45.0	8
CGACTCA	20	7.03255E-4	45.0	10
TCGACGT	20	7.03255E-4	45.0	26
AATACGG	20	7.03255E-4	45.0	33
ATACCGG	20	7.03255E-4	45.0	2
CGCCCGT	20	7.03255E-4	45.0	21
AGTCGAG	20	7.03255E-4	45.0	29
TGGGCGA	1165	0.0	42.875538	6
TTGGGCG	875	0.0	41.657143	5
TGTTTCG	180	0.0	41.25	1
TTTGGGA	6970	0.0	40.480633	4
TTTTTCG	585	0.0	40.384617	1
>>END_MODULE