FastQCFastQC Report
Sat 14 Jan 2017
SRR2933970.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933970.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726833
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA86440.500569539729667No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT66940.3876460549456722No Hit
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC58130.3366278036150572Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT52320.3029823960973644Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC45930.2659782387758399Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC40400.23395429668068654Illumina Single End Adapter 1 (95% over 21bp)
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATG37350.21629190547088226No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTA35370.20482582855435352No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCGTC32480.18808998901457177No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.14986973262614278No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTC18860.10921727810390466No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC18370.10637971361446069No Hit
GCTCCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC18040.10446870079503924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA406.8193913E-945.022
CTACGTT207.0342724E-445.030
GCTCAAC40600.042.506161
CTTGACC39500.042.265827
TTGACCC34650.042.0779238
CGAATAT1500.042.014
CATTAGA28700.041.70731717
TCAACTT38400.041.6601523
CTCAACT39550.041.472822
ACTTGAC38250.041.4705926
TGCATTA29250.041.07692315
AACTTGA38250.041.0588265
CCTGCAT30800.040.9821413
GCATTAG29900.040.4849516
ATTAGAA29500.040.34745818
CCCTGCA31550.040.15055512
CGTAGTC451.9292202E-840.05
ACCCTGC32000.039.72656211
CAACTTG39900.039.4736824
TGACCCT32400.039.4444439
Produced by FastQC (version 0.11.5)