##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933970.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1726833
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4640801976798	33.0	31.0	34.0	31.0	34.0
2	32.695912691036135	34.0	31.0	34.0	31.0	34.0
3	32.76627792033161	34.0	31.0	34.0	31.0	34.0
4	36.11212896672695	37.0	35.0	37.0	35.0	37.0
5	36.12056290330333	37.0	35.0	37.0	35.0	37.0
6	35.65651166036322	37.0	35.0	37.0	35.0	37.0
7	36.05443085695027	37.0	35.0	37.0	35.0	37.0
8	36.089872616518214	37.0	36.0	37.0	35.0	37.0
9	37.95837524531903	39.0	38.0	39.0	35.0	39.0
10	37.648397384113	39.0	37.0	39.0	35.0	39.0
11	37.59278749016263	39.0	37.0	39.0	35.0	39.0
12	37.393936761690334	39.0	37.0	39.0	35.0	39.0
13	37.32261370960597	39.0	37.0	39.0	35.0	39.0
14	38.41930748369993	40.0	38.0	41.0	34.0	41.0
15	38.53110520820485	40.0	38.0	41.0	34.0	41.0
16	38.644773987988415	40.0	38.0	41.0	35.0	41.0
17	38.642780164613484	40.0	38.0	41.0	35.0	41.0
18	38.56569743571034	40.0	38.0	41.0	35.0	41.0
19	38.586891726067314	40.0	38.0	41.0	35.0	41.0
20	38.53937873552336	40.0	38.0	41.0	34.0	41.0
21	38.44437997189074	40.0	38.0	41.0	34.0	41.0
22	38.47508763151966	40.0	38.0	41.0	34.0	41.0
23	38.43569644545825	40.0	37.0	41.0	34.0	41.0
24	38.40136481060994	40.0	37.0	41.0	34.0	41.0
25	38.327358233251275	40.0	37.0	41.0	34.0	41.0
26	38.2600384634762	40.0	37.0	41.0	34.0	41.0
27	38.23488953477261	40.0	37.0	41.0	34.0	41.0
28	38.15547710751416	40.0	37.0	41.0	34.0	41.0
29	38.115346417401106	40.0	37.0	41.0	34.0	41.0
30	38.02512808129101	40.0	37.0	41.0	34.0	41.0
31	37.94961412018417	40.0	37.0	41.0	34.0	41.0
32	37.86860281220014	40.0	36.0	41.0	33.0	41.0
33	37.77948533529299	40.0	36.0	41.0	33.0	41.0
34	37.649165842904324	40.0	36.0	41.0	33.0	41.0
35	37.553947023250075	40.0	36.0	41.0	33.0	41.0
36	37.407383921896326	40.0	35.0	41.0	32.0	41.0
37	37.30377459777523	40.0	35.0	41.0	32.0	41.0
38	37.270258907491346	40.0	35.0	41.0	32.0	41.0
39	37.237500094102906	40.0	35.0	41.0	32.0	41.0
40	37.131825138852456	39.0	35.0	41.0	32.0	41.0
41	37.04701207354736	39.0	35.0	41.0	32.0	41.0
42	37.05634766071763	39.0	35.0	41.0	32.0	41.0
43	36.91402932420217	39.0	35.0	41.0	32.0	41.0
44	36.781015303738116	39.0	35.0	41.0	31.0	41.0
45	36.7989579768281	39.0	35.0	41.0	31.0	41.0
46	36.77886802024284	39.0	35.0	41.0	31.0	41.0
47	36.7148878901434	39.0	35.0	41.0	31.0	41.0
48	36.653136116810366	39.0	35.0	41.0	31.0	41.0
49	36.617293044550344	39.0	35.0	41.0	31.0	41.0
50	36.52796998899141	39.0	35.0	41.0	31.0	41.0
51	35.631992207700456	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	41.0
10	53.0
11	46.0
12	31.0
13	22.0
14	32.0
15	44.0
16	110.0
17	143.0
18	290.0
19	474.0
20	843.0
21	1386.0
22	2107.0
23	3064.0
24	4483.0
25	6621.0
26	9296.0
27	11084.0
28	13280.0
29	15988.0
30	20461.0
31	26427.0
32	36047.0
33	53769.0
34	105651.0
35	117094.0
36	122295.0
37	198367.0
38	366007.0
39	610828.0
40	436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.867411614209363	4.725645155032362	53.43944666334266	15.96749656741561
2	19.688412255267302	4.299952572136391	51.89702768015205	24.11460749244426
3	21.407397241076584	4.413976337028537	52.575610959484784	21.603015462410088
4	19.364524537115056	4.875341159220376	49.10191083909098	26.658223464573588
5	18.584252211997338	8.201256288245592	48.461895273022925	24.75259622673414
6	21.851041762579243	8.743115286770637	50.96804381199572	18.437799138654405
7	82.79851033655252	2.3222280324733195	8.024342828750667	6.854918802223492
8	84.1264905176123	2.8498992085511454	8.668817424730706	4.3547928491058485
9	78.45888977104329	5.370235569971156	11.046928104802259	5.123946554183294
10	39.36391069663367	31.37888840437958	15.53097491187625	13.726225987110507
11	30.163484251227533	26.181223082950115	24.87675415051716	18.778538515305186
12	27.5884813412762	21.967034449770185	28.319414789965215	22.125069418988403
13	26.93346722004965	21.484532667605958	29.319743136713278	22.26225697563111
14	21.627047896351296	22.55823232472393	31.642492354500984	24.172227424423788
15	19.365393179305702	23.436719126864034	34.886407660729205	22.31148003310106
16	25.685112573132436	23.521324876232967	29.514550625335513	21.279011925299088
17	24.896211735587634	23.674090082827927	27.396337688705273	24.03336049287916
18	24.126421026237047	24.851679345947176	30.104358672784226	20.91754095503155
19	24.336632436373407	25.29364449254792	29.53325538717409	20.83646768390458
20	25.18541167559341	27.32180818874784	29.288529927329392	18.204250208329352
21	25.621759602694645	27.287178320080752	28.883858485447057	18.20720359177755
22	25.844247822458797	23.34765434758312	29.386396947475525	21.421700882482554
23	23.212956898553593	25.464940732543333	29.56811689375869	21.753985475144383
24	22.984388183454914	26.681329346844773	28.072836226780467	22.261446242919842
25	23.01282173782873	26.736343352252362	25.85768282167413	24.393152088244783
26	22.327463049408948	25.73595709602492	27.852201110356354	24.084378744209776
27	20.969775305429074	24.823361610532114	30.933043322660613	23.2738197613782
28	20.236930843920632	25.319008844514784	28.953234041739996	25.490826269824584
29	20.88488000866326	26.942269460914865	28.265211517268895	23.907639013152977
30	22.59778449913802	26.518314162400188	28.57630124047896	22.30760009798284
31	23.171204163923207	26.4674696395077	27.637878127184273	22.723448069384823
32	25.02633433574642	27.3746795434185	25.202668700447582	22.396317420387497
33	24.57724632318238	25.500555062359826	26.269361310561013	23.65283730389679
34	22.019789985482095	25.593268138841452	29.271157083516474	23.11578479215998
35	23.994850689093848	27.14576337144356	26.607263122722348	22.25212281674024
36	24.088258679327996	27.031739606551415	26.695459259812615	22.184542454307973
37	21.99436772403585	28.76358049678226	26.76877266070315	22.473279118478743
38	22.563907453702818	28.328217030830427	25.62888246865794	23.47899304680881
39	22.55603176450763	27.143620720706636	26.433766322510632	23.866581192275106
40	23.962305561684307	25.673009491942768	28.83179786348767	21.532887082885257
41	22.49736946189933	25.619269495081458	27.079283289119445	24.80407775389977
42	23.437877316451562	23.429827898818242	28.00734060560575	25.124954179124444
43	23.027530745590337	23.844807228029577	28.88194747262764	24.24571455375245
44	22.82936450716427	24.257528087545236	27.965877418372248	24.94722998691825
45	22.481791811947076	24.083625920977884	26.945222844363066	26.489359422711985
46	22.341998328732426	24.226836063475737	28.533737773137297	24.897427834654536
47	20.777689562337528	24.599599382221673	30.861640934589506	23.761070120851294
48	20.5214980255763	25.643649385898925	29.54842767077071	24.28642491775406
49	23.09679048292452	24.65849332274748	28.69136737599988	23.553348818328118
50	21.262102357321176	25.27314453684867	28.379061553722913	25.085691552107235
51	21.041467240897063	24.83129520920668	26.34197979769902	27.78525775219723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	296.0
1	977.0
2	1658.0
3	3349.0
4	5040.0
5	3550.0
6	2060.0
7	2052.5
8	2045.0
9	2184.5
10	2324.0
11	2306.0
12	2288.0
13	2349.5
14	2411.0
15	2405.0
16	2399.0
17	2418.5
18	2438.0
19	2772.5
20	3107.0
21	3948.0
22	4789.0
23	5862.0
24	6935.0
25	8093.5
26	12100.0
27	14948.0
28	18021.5
29	21095.0
30	28557.0
31	36019.0
32	36285.0
33	36551.0
34	42086.0
35	47621.0
36	51429.5
37	55238.0
38	64291.0
39	73344.0
40	77734.0
41	82124.0
42	86625.5
43	91127.0
44	98569.5
45	106012.0
46	123167.0
47	140322.0
48	158346.0
49	176370.0
50	174350.0
51	172330.0
52	149763.0
53	127196.0
54	114990.0
55	102784.0
56	95574.0
57	88364.0
58	83514.0
59	78664.0
60	73506.0
61	68348.0
62	60688.5
63	53029.0
64	47228.0
65	41427.0
66	34501.5
67	27576.0
68	22263.5
69	16951.0
70	12995.0
71	9039.0
72	7479.0
73	5919.0
74	4900.5
75	2930.5
76	1979.0
77	1474.5
78	970.0
79	646.5
80	323.0
81	235.5
82	148.0
83	104.5
84	61.0
85	39.0
86	17.0
87	11.0
88	5.0
89	5.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1726833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.52602624671856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21679528514191	18.625725546941386
2	11.689557555512765	6.2015502106011775
3	4.534502402005977	3.6084698919425673
4	2.2074947616797163	2.3422425595123952
5	1.298921109348065	1.7227607719491782
6	0.8564734171497985	1.3631301805759375
7	0.6592673500643568	1.2241420121988205
8	0.5014928693794182	1.064209041256052
9	0.4224036833190688	1.0084222071388993
>10	7.0571981992220065	47.66597843081895
>50	0.4757809747563656	7.562122399091997
>100	0.07236672276010199	3.2850423141986456
>500	0.0030982678641631916	0.5502267987104342
>1k	0.003762182406483875	2.227206309940667
>5k	8.852193897609117E-4	1.5487713251229474
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	8644	0.500569539729667	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	6694	0.3876460549456722	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	5813	0.3366278036150572	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	5232	0.3029823960973644	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	4593	0.2659782387758399	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	4040	0.23395429668068654	Illumina Single End Adapter 1 (95% over 21bp)
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATG	3735	0.21629190547088226	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTA	3537	0.20482582855435352	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCGTC	3248	0.18808998901457177	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2588	0.14986973262614278	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTC	1886	0.10921727810390466	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	1837	0.10637971361446069	No Hit
GCTCCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	1804	0.10446870079503924	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7372843812922268E-4	0.0	0.0	0.3187337744877472	0.0
2	1.7372843812922268E-4	0.0	0.0	1.2217741958834467	0.0
3	1.7372843812922268E-4	0.0	0.0	1.7754467282012794	0.0
4	1.7372843812922268E-4	0.0	0.0	2.299411697599015	0.0
5	1.7372843812922268E-4	0.0	0.0	3.2665000031850213	0.0
6	1.7372843812922268E-4	0.0	0.0	3.8616936322157382	0.0
7	1.7372843812922268E-4	0.0	0.0	4.3762193564751195	0.0
8	1.7372843812922268E-4	0.0	0.0	5.292115682292382	0.0
9	1.7372843812922268E-4	0.0	0.0	5.71045376130755	0.0
10	1.7372843812922268E-4	0.0	0.0	6.509372938784469	0.0
11	1.7372843812922268E-4	0.0	0.0	8.019999617797437	0.0
12	1.7372843812922268E-4	0.0	0.0	9.401256519883509	0.0
13	1.7372843812922268E-4	0.0	0.0	10.09454880697786	0.0
14	1.7372843812922268E-4	0.0	0.0	10.271867632828421	0.0
15	1.7372843812922268E-4	0.0	0.0	10.759754996574655	0.0
16	2.3163791750563026E-4	0.0	0.0	11.334390760426746	0.0
17	2.3163791750563026E-4	0.0	0.0	12.09647950902027	0.0
18	2.3163791750563026E-4	0.0	0.0	12.860131813556956	0.0
19	2.3163791750563026E-4	0.0	0.0	13.454688438314532	0.0
20	2.3163791750563026E-4	0.0	0.0	14.182784322514106	0.0
21	2.3163791750563026E-4	0.0	0.0	15.139391012332982	0.0
22	2.8954739688203784E-4	0.0	0.0	16.140356363354186	0.0
23	2.8954739688203784E-4	0.0	0.0	17.09012973460665	0.0
24	2.8954739688203784E-4	0.0	0.0	17.789965792870532	0.0
25	2.8954739688203784E-4	0.0	0.0	18.64951619525455	0.0
26	2.8954739688203784E-4	0.0	0.0	19.243899091573997	0.0
27	2.8954739688203784E-4	0.0	0.0	19.866657632787884	0.0
28	2.8954739688203784E-4	0.0	0.0	20.453686025226528	0.0
29	3.4745687625844536E-4	0.0	0.0	21.10279337955668	0.0
30	3.4745687625844536E-4	0.0	0.0	21.820870923824135	0.0
31	3.4745687625844536E-4	0.0	0.0	22.430136556343317	0.0
32	3.4745687625844536E-4	0.0	0.0	23.125397765736466	0.0
33	3.4745687625844536E-4	0.0	0.0	23.710283507438184	0.0
34	3.4745687625844536E-4	0.0	0.0	24.45088783918306	0.0
35	3.4745687625844536E-4	0.0	0.0	25.1027169390439	0.0
36	4.0536635563485294E-4	0.0	0.0	25.688645051374394	0.0
37	4.0536635563485294E-4	0.0	0.0	26.30966630820699	0.0
38	4.632758350112605E-4	0.0	0.0	26.983848467107126	0.0
39	5.211853143876681E-4	0.0	0.0	28.083607389944483	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	40	6.8193913E-9	45.0	22
CTACGTT	20	7.0342724E-4	45.0	30
GCTCAAC	4060	0.0	42.50616	1
CTTGACC	3950	0.0	42.26582	7
TTGACCC	3465	0.0	42.077923	8
CGAATAT	150	0.0	42.0	14
CATTAGA	2870	0.0	41.707317	17
TCAACTT	3840	0.0	41.660152	3
CTCAACT	3955	0.0	41.47282	2
ACTTGAC	3825	0.0	41.470592	6
TGCATTA	2925	0.0	41.076923	15
AACTTGA	3825	0.0	41.058826	5
CCTGCAT	3080	0.0	40.98214	13
GCATTAG	2990	0.0	40.48495	16
ATTAGAA	2950	0.0	40.347458	18
CCCTGCA	3155	0.0	40.150555	12
CGTAGTC	45	1.9292202E-8	40.0	5
ACCCTGC	3200	0.0	39.726562	11
CAACTTG	3990	0.0	39.473682	4
TGACCCT	3240	0.0	39.444443	9
>>END_MODULE