FastQCFastQC Report
Sat 14 Jan 2017
SRR2933969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913593
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC93411.022446538009814No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT85180.9323626603969163Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC61750.6759027269254471No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC55350.6058496507744696No Hit
TCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC53880.5897593348460419No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG48310.5287912670083944No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC46730.5114969138336218No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG30990.3392101296748114No Hit
TCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG20370.22296580643678313No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC18690.20457687394715152No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC17720.193959454593019No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.1713016627754372No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCTCGGT13080.1431709743835603No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT12740.13944940471303963No Hit
TTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT12020.13156843364605464No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC11510.12598607914027363No Hit
GCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG11480.1256577053458159No Hit
TGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG11140.12193613567529525No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTA10500.11493082806019747No Hit
CGTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT10420.11405516460831026No Hit
GAACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT10250.11219437977304993No Hit
TTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG9810.10737823078767021No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA207.032525E-445.018
TACGTCG207.032525E-445.012
TTTCGCG3300.042.2727281
CGTTTTT12900.041.162791
CGGGTAT1100.040.9090926
TTTGTCG2150.040.8139531
CTTTGCG2800.040.178571
TGGGCGA10350.039.9999966
CTCGTTG1200.039.3749961
TTCGTTG3850.039.1558461
ACGCCGG750.039.027
CGGGCAC1750.038.571436
CGGCGAA356.2468825E-638.57142631
GCGATAT356.2468825E-638.5714269
TAGTGCG356.2468825E-638.5714261
GCGATCT3100.038.467749
TGCGGGC3000.038.254
ACGGGAT1650.038.181825
GGGCGAT15950.037.946717
TCTACGG950.037.8947372
Produced by FastQC (version 0.11.5)