##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933969.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	913593
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.424569802964776	33.0	31.0	34.0	31.0	34.0
2	32.647726066202345	34.0	31.0	34.0	31.0	34.0
3	32.70774951209127	34.0	31.0	34.0	31.0	34.0
4	36.08949389936219	37.0	35.0	37.0	35.0	37.0
5	36.1241241997257	37.0	35.0	37.0	35.0	37.0
6	35.687990166299436	37.0	35.0	37.0	35.0	37.0
7	36.04384556361531	37.0	35.0	37.0	35.0	37.0
8	36.01513912650382	37.0	36.0	37.0	35.0	37.0
9	37.895832170342814	39.0	38.0	39.0	35.0	39.0
10	37.62885551881418	39.0	37.0	39.0	35.0	39.0
11	37.579829311301644	39.0	37.0	39.0	35.0	39.0
12	37.53476438632958	39.0	37.0	39.0	35.0	39.0
13	37.516874582007524	39.0	37.0	39.0	35.0	39.0
14	38.79703872512158	40.0	38.0	41.0	35.0	41.0
15	38.826193939752166	40.0	38.0	41.0	35.0	41.0
16	38.89289869777899	40.0	38.0	41.0	35.0	41.0
17	38.83704888281762	40.0	38.0	41.0	35.0	41.0
18	38.80225220639825	40.0	38.0	41.0	35.0	41.0
19	38.838335013512584	40.0	38.0	41.0	35.0	41.0
20	38.7665360833544	40.0	38.0	41.0	35.0	41.0
21	38.7197220206372	40.0	38.0	41.0	35.0	41.0
22	38.69866559835725	40.0	38.0	41.0	35.0	41.0
23	38.634857097197546	40.0	38.0	41.0	35.0	41.0
24	38.60528922616526	40.0	38.0	41.0	35.0	41.0
25	38.514168781941194	40.0	38.0	41.0	34.0	41.0
26	38.41157933565603	40.0	38.0	41.0	34.0	41.0
27	38.37961543050352	40.0	38.0	41.0	34.0	41.0
28	38.34517668152011	40.0	38.0	41.0	34.0	41.0
29	38.32837269987839	40.0	38.0	41.0	34.0	41.0
30	38.30349400663096	40.0	38.0	41.0	34.0	41.0
31	38.280530827184535	40.0	38.0	41.0	34.0	41.0
32	38.158090090445086	40.0	38.0	41.0	34.0	41.0
33	38.077712942196364	40.0	38.0	41.0	34.0	41.0
34	38.00038091360157	40.0	37.0	41.0	34.0	41.0
35	37.94261886857714	40.0	37.0	41.0	34.0	41.0
36	37.83067295830857	40.0	37.0	41.0	33.0	41.0
37	37.71226465176506	40.0	37.0	41.0	33.0	41.0
38	37.72465528960927	40.0	37.0	41.0	33.0	41.0
39	37.72964000380914	40.0	37.0	41.0	33.0	41.0
40	37.604425603085836	40.0	37.0	41.0	33.0	41.0
41	37.505322391918504	40.0	37.0	41.0	33.0	41.0
42	37.47396050538916	40.0	36.0	41.0	33.0	41.0
43	37.335209442279	40.0	36.0	41.0	32.0	41.0
44	37.217023335336414	39.0	36.0	41.0	32.0	41.0
45	37.26531289097005	40.0	36.0	41.0	32.0	41.0
46	37.201822912390966	39.0	36.0	41.0	32.0	41.0
47	37.111319810900476	39.0	36.0	41.0	32.0	41.0
48	37.08237585007766	39.0	35.0	41.0	32.0	41.0
49	37.06852832716538	39.0	35.0	41.0	32.0	41.0
50	36.99128167575715	39.0	35.0	41.0	32.0	41.0
51	36.048821521180656	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	18.0
10	25.0
11	16.0
12	11.0
13	20.0
14	16.0
15	19.0
16	30.0
17	64.0
18	101.0
19	232.0
20	491.0
21	779.0
22	1190.0
23	1747.0
24	2345.0
25	3298.0
26	4345.0
27	5424.0
28	6383.0
29	7677.0
30	9572.0
31	12490.0
32	16371.0
33	22969.0
34	39714.0
35	54089.0
36	61475.0
37	100596.0
38	203545.0
39	358310.0
40	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.788726489804542	4.285059101810106	51.57975159617029	18.346462812215066
2	18.61222666986284	7.616849078309489	51.54176969394467	22.229154557882996
3	19.30783182445575	7.536397498667349	51.39367311264426	21.76209756423265
4	18.394186470342923	4.397362939514641	54.09181112377175	23.116639466370692
5	20.095819473222758	5.37110069801323	50.14289732955485	24.390182499209168
6	20.67835458459073	8.11871369417235	52.123210225997795	19.079721495239127
7	79.18613649622972	1.9441917790525978	13.00119418603251	5.868477538685169
8	79.4176400213224	4.8360703289101385	9.259155882323968	6.48713376744349
9	74.19551156806149	5.3423132620324365	11.656831871522657	8.805343298383415
10	33.30618776632483	36.3189078725428	17.521916214331764	12.8529881468006
11	24.49372970239483	25.316415515442873	30.79861601391429	19.391238768248005
12	23.6189419139595	22.134801820942148	33.1914758541276	21.05478041097075
13	22.931217730433573	22.69840071016306	33.5230239285984	20.847357630804964
14	17.692670587449772	28.36569457077714	29.826082292662047	24.115552549111037
15	15.829915509422687	27.63374938293091	35.20429775622186	21.33203735142454
16	17.965549210644127	26.82802954926318	31.29851038701041	23.90791085308228
17	17.752544075972562	27.547934364645965	30.54029529560756	24.15922626377391
18	17.785709829212788	27.20226621701348	31.75878098890863	23.2532429648651
19	19.97848057066987	26.59707331382793	30.179740869293	23.2447052462092
20	23.288269502940587	26.69033147145392	31.379837630104433	18.64156139550106
21	23.925424122120027	27.301872934665656	29.218152941189345	19.554550002024975
22	21.002021687994546	25.690871099056146	29.475488538112703	23.83161867483661
23	21.12286324435498	27.195589283192845	28.955125531828724	22.72642194062345
24	22.329855854850024	24.16973422519656	28.4840185947134	25.01639132524001
25	19.9448769857037	28.916596339945688	26.40650705511097	24.73201961923964
26	19.424623437351205	30.76019627996274	27.049462944659165	22.76571733802689
27	19.86639564882831	31.241263888843278	28.148311118846138	20.744029343482275
28	16.951859307153185	29.063051052273824	31.346781334795693	22.6383083057773
29	18.59350936357875	27.207848571519268	29.83254031061972	24.36610175428227
30	18.93567485740368	27.904767221290005	30.507895747887737	22.65166217341858
31	19.63795694581723	27.776810899382987	27.667790799623027	24.91744135517676
32	22.61291406567257	28.8007898484336	25.836012316206453	22.75028376968738
33	22.55358786680721	26.395999093688328	27.854854404532436	23.195558634972027
34	21.12450511332727	25.601115595237705	28.63342867119166	24.64095062024337
35	21.627026476779047	25.697657490808268	26.869404647364853	25.805911385047825
36	21.753231471782293	27.831868238920393	28.24682325718345	22.16807703211386
37	19.936996014636716	28.383207839814883	30.152814218147466	21.526981927400932
38	20.22300958960938	25.91055316754835	29.70852447424619	24.15791276859608
39	22.171798601784385	24.08687457106173	29.763362897920626	23.97796392923326
40	22.396077903398997	23.80644335059485	31.00111318716321	22.796365558842943
41	20.532666077782995	26.462549516031757	27.478756951946874	25.526027454238374
42	23.523166223909335	25.522196426636366	27.33886971550789	23.615767633946408
43	23.9364793732001	25.452471724279853	27.725037297790156	22.886011604729898
44	20.56189134548973	25.183861960413445	29.15269709816078	25.101549595936046
45	20.166200923168194	23.49952331070838	28.244086808896302	28.090188957227124
46	21.124942945053213	25.29747929329581	29.518067673460717	24.059510088190255
47	18.706251033009227	24.905838814439253	32.735693027420304	23.652217125131212
48	19.44826635055216	23.660864301718597	30.680620363772487	26.210248983956753
49	20.22530820617058	22.69916691568346	32.98481927948222	24.090705598663735
50	20.850860284612512	22.495903536914142	29.975820742934765	26.67741543553858
51	19.16652163490745	22.14815568858343	28.279222804903277	30.406099871605846
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	221.0
1	574.5
2	928.0
3	2024.0
4	3120.0
5	2257.0
6	1394.0
7	1511.0
8	1628.0
9	1780.5
10	1933.0
11	2013.0
12	2093.0
13	2053.5
14	2014.0
15	2009.5
16	2005.0
17	2172.0
18	2339.0
19	2688.5
20	3038.0
21	3403.0
22	3768.0
23	4498.0
24	5228.0
25	6182.0
26	9156.0
27	11176.0
28	12674.5
29	14173.0
30	16430.0
31	18687.0
32	21018.0
33	23349.0
34	26480.0
35	29611.0
36	32452.5
37	35294.0
38	38164.5
39	41035.0
40	44659.0
41	48283.0
42	51109.5
43	53936.0
44	59093.5
45	64251.0
46	82259.0
47	100267.0
48	104374.0
49	108481.0
50	101127.0
51	93773.0
52	74847.5
53	55922.0
54	47372.0
55	38822.0
56	35964.5
57	33107.0
58	32110.0
59	31113.0
60	29731.5
61	28350.0
62	23959.5
63	19569.0
64	15355.5
65	11142.0
66	8817.0
67	6492.0
68	5083.5
69	3675.0
70	3197.0
71	2719.0
72	2213.0
73	1707.0
74	1262.0
75	678.0
76	539.0
77	406.5
78	274.0
79	195.0
80	116.0
81	80.0
82	44.0
83	27.5
84	11.0
85	9.5
86	8.0
87	5.0
88	2.0
89	1.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	913593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.222062456172857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.52428072535602	22.643576223960793
2	10.496322893473405	6.554336978803672
3	3.528095473356486	3.304632516614308
4	1.7195786656347485	2.1475516998700197
5	1.075563918930869	1.6790661926232815
6	0.892026256915814	1.6710539703582983
7	0.7075390242380767	1.5463579323458583
8	0.645067810568034	1.6112277976017468
9	0.5859441029021808	1.6464945038974257
>10	7.64356130209096	43.53253882845863
>50	0.11686098548204643	2.513250153563523
>100	0.05347274455849664	3.1036661558889436
>500	0.004249489633787221	0.8465951250628693
>1k	0.005665986178382961	3.334636146257821
>5k	0.0017706206807446753	3.865015774692794
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	9341	1.022446538009814	No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	8518	0.9323626603969163	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	6175	0.6759027269254471	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	5535	0.6058496507744696	No Hit
TCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	5388	0.5897593348460419	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	4831	0.5287912670083944	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	4673	0.5114969138336218	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	3099	0.3392101296748114	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	2037	0.22296580643678313	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC	1869	0.20457687394715152	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	1772	0.193959454593019	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1565	0.1713016627754372	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCTCGGT	1308	0.1431709743835603	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT	1274	0.13944940471303963	No Hit
TTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	1202	0.13156843364605464	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC	1151	0.12598607914027363	No Hit
GCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	1148	0.1256577053458159	No Hit
TGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	1114	0.12193613567529525	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTA	1050	0.11493082806019747	No Hit
CGTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	1042	0.11405516460831026	No Hit
GAACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	1025	0.11219437977304993	No Hit
TTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	981	0.10737823078767021	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0945793148590236E-4	0.0	0.0	0.9841362619897481	0.0
2	1.0945793148590236E-4	0.0	0.0	3.3848770732700446	0.0
3	1.0945793148590236E-4	0.0	0.0	4.657653900588118	0.0
4	1.0945793148590236E-4	0.0	0.0	5.88850834014709	0.0
5	1.0945793148590236E-4	0.0	0.0	8.100653135477176	0.0
6	1.0945793148590236E-4	0.0	0.0	8.969201821817812	0.0
7	1.0945793148590236E-4	0.0	0.0	9.931227581647407	0.0
8	1.0945793148590236E-4	0.0	0.0	11.198203138596728	0.0
9	1.0945793148590236E-4	0.0	0.0	11.729074106303354	0.0
10	1.0945793148590236E-4	0.0	0.0	13.260828399517072	0.0
11	1.0945793148590236E-4	0.0	0.0	15.066665353171489	0.0
12	1.0945793148590236E-4	0.0	0.0	17.34579840257095	0.0
13	1.0945793148590236E-4	0.0	0.0	17.94519003538775	0.0
14	1.0945793148590236E-4	0.0	0.0	18.189719054327256	0.0
15	1.0945793148590236E-4	0.0	0.0	18.627550780270866	0.0
16	1.0945793148590236E-4	0.0	0.0	19.36704856538962	0.0
17	1.0945793148590236E-4	0.0	0.0	20.397813906192365	0.0
18	1.0945793148590236E-4	0.0	0.0	21.390159513043553	0.0
19	1.0945793148590236E-4	0.0	0.0	22.281256533270284	0.0
20	1.0945793148590236E-4	0.0	0.0	23.046367474356742	0.0
21	1.0945793148590236E-4	0.0	0.0	23.95782366983985	0.0
22	1.0945793148590236E-4	0.0	0.0	24.98399177752019	0.0
23	1.0945793148590236E-4	0.0	0.0	25.895010141277353	0.0
24	1.0945793148590236E-4	0.0	0.0	26.58776938965163	0.0
25	1.0945793148590236E-4	0.0	0.0	27.1850813217702	0.0
26	1.0945793148590236E-4	0.0	0.0	27.721534643982604	0.0
27	1.0945793148590236E-4	0.0	0.0	28.267291890371315	0.0
28	1.0945793148590236E-4	0.0	0.0	28.814253174006367	0.0
29	1.0945793148590236E-4	0.0	0.0	29.386499239814665	0.0
30	1.0945793148590236E-4	0.0	0.0	30.010737823078767	0.0
31	1.0945793148590236E-4	0.0	0.0	30.60553222277316	0.0
32	1.0945793148590236E-4	0.0	0.0	31.17208647614419	0.0
33	2.1891586297180472E-4	0.0	0.0	31.680299652033234	0.0
34	2.1891586297180472E-4	0.0	0.0	32.20066265831721	0.0
35	2.1891586297180472E-4	0.0	0.0	32.72058783287525	0.0
36	2.1891586297180472E-4	0.0	0.0	33.24959801574662	0.0
37	2.1891586297180472E-4	0.0	0.0	33.782001394494046	0.0
38	2.1891586297180472E-4	0.0	0.0	34.283975468288396	0.0
39	2.1891586297180472E-4	0.0	0.0	34.791094064862584	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTAA	20	7.032525E-4	45.0	18
TACGTCG	20	7.032525E-4	45.0	12
TTTCGCG	330	0.0	42.272728	1
CGTTTTT	1290	0.0	41.16279	1
CGGGTAT	110	0.0	40.909092	6
TTTGTCG	215	0.0	40.813953	1
CTTTGCG	280	0.0	40.17857	1
TGGGCGA	1035	0.0	39.999996	6
CTCGTTG	120	0.0	39.374996	1
TTCGTTG	385	0.0	39.155846	1
ACGCCGG	75	0.0	39.0	27
CGGGCAC	175	0.0	38.57143	6
CGGCGAA	35	6.2468825E-6	38.571426	31
GCGATAT	35	6.2468825E-6	38.571426	9
TAGTGCG	35	6.2468825E-6	38.571426	1
GCGATCT	310	0.0	38.46774	9
TGCGGGC	300	0.0	38.25	4
ACGGGAT	165	0.0	38.18182	5
GGGCGAT	1595	0.0	37.94671	7
TCTACGG	95	0.0	37.894737	2
>>END_MODULE