##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933968.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1582751
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37795269123191	33.0	31.0	34.0	31.0	34.0
2	32.6021193478949	34.0	31.0	34.0	31.0	34.0
3	32.65518012624854	34.0	31.0	34.0	31.0	34.0
4	36.04591120144609	37.0	35.0	37.0	35.0	37.0
5	36.094958714289234	37.0	35.0	37.0	35.0	37.0
6	35.77468281492161	37.0	35.0	37.0	35.0	37.0
7	36.09840461323354	37.0	35.0	37.0	35.0	37.0
8	36.104590677876686	37.0	36.0	37.0	35.0	37.0
9	37.92341751797977	39.0	38.0	39.0	35.0	39.0
10	37.525582356289775	39.0	37.0	39.0	35.0	39.0
11	37.60942055951947	39.0	37.0	39.0	35.0	39.0
12	37.46772233914242	39.0	37.0	39.0	35.0	39.0
13	37.43269724675581	39.0	37.0	39.0	35.0	39.0
14	38.64957280077536	40.0	38.0	41.0	35.0	41.0
15	38.678094659235725	40.0	38.0	41.0	35.0	41.0
16	38.71545239901918	40.0	38.0	41.0	35.0	41.0
17	38.681288465462984	40.0	38.0	41.0	35.0	41.0
18	38.63929512601793	40.0	38.0	41.0	35.0	41.0
19	38.67528815334819	40.0	38.0	41.0	35.0	41.0
20	38.64473438967974	40.0	38.0	41.0	35.0	41.0
21	38.57235092569836	40.0	38.0	41.0	34.0	41.0
22	38.572968837170215	40.0	38.0	41.0	34.0	41.0
23	38.50596966926573	40.0	38.0	41.0	34.0	41.0
24	38.49591502390458	40.0	38.0	41.0	34.0	41.0
25	38.38718598187586	40.0	38.0	41.0	34.0	41.0
26	38.33873047624042	40.0	38.0	41.0	34.0	41.0
27	38.305670317061875	40.0	38.0	41.0	34.0	41.0
28	38.23227721858966	40.0	37.0	41.0	34.0	41.0
29	38.254999680935285	40.0	38.0	41.0	34.0	41.0
30	38.16636287072319	40.0	37.0	41.0	34.0	41.0
31	38.11898776244652	40.0	37.0	41.0	34.0	41.0
32	38.052464348466685	40.0	37.0	41.0	34.0	41.0
33	37.92608312994274	40.0	37.0	41.0	33.0	41.0
34	37.92444737043287	40.0	37.0	41.0	33.0	41.0
35	37.87472508309899	40.0	37.0	41.0	33.0	41.0
36	37.71283670015056	40.0	37.0	41.0	33.0	41.0
37	37.649714326511244	40.0	37.0	41.0	33.0	41.0
38	37.66898393998803	40.0	37.0	41.0	33.0	41.0
39	37.675600899952045	40.0	37.0	41.0	33.0	41.0
40	37.61335990310542	40.0	37.0	41.0	33.0	41.0
41	37.464507051330244	40.0	36.0	41.0	33.0	41.0
42	37.36228629771834	40.0	36.0	41.0	32.0	41.0
43	37.20357150303491	39.0	36.0	41.0	32.0	41.0
44	37.13548751509239	39.0	36.0	41.0	32.0	41.0
45	37.182892950312464	39.0	36.0	41.0	32.0	41.0
46	37.07450824545364	39.0	35.0	41.0	31.0	41.0
47	36.96333409361296	39.0	35.0	41.0	31.0	41.0
48	36.882924730421905	39.0	35.0	41.0	31.0	41.0
49	36.871799164871796	39.0	35.0	41.0	31.0	41.0
50	36.80128207153241	39.0	35.0	41.0	31.0	41.0
51	35.79637858387074	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	10.0
10	23.0
11	26.0
12	11.0
13	18.0
14	17.0
15	29.0
16	43.0
17	80.0
18	134.0
19	293.0
20	564.0
21	913.0
22	1434.0
23	2238.0
24	3275.0
25	5089.0
26	7272.0
27	9236.0
28	11394.0
29	13935.0
30	17857.0
31	23029.0
32	30504.0
33	43356.0
34	75212.0
35	100912.0
36	114193.0
37	189330.0
38	375171.0
39	556877.0
40	274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.535446194632005	4.604009095555775	58.33368609465418	14.526858615158039
2	18.97367305406852	4.160288004872529	55.494641924092925	21.37139701696603
3	20.184602631746877	4.206915680356544	53.716724867019515	21.891756820877067
4	19.142556220150865	4.51233327289005	52.41765761007259	23.927452896886496
5	17.796039933002728	5.304814212722026	52.11426181376603	24.784884040509215
6	21.75907644348353	5.863967231737652	53.30269890841958	19.074257416359238
7	83.84837539195995	1.627230057033608	9.6517392817948	4.872655269211645
8	85.07724841115248	2.250196019462316	7.233481450967334	5.439074118417868
9	78.4002663716529	6.181894688425406	9.584640919512925	5.833198020408769
10	27.440387022342744	45.053580759070755	14.583405728380521	12.922626490205976
11	28.901703426502333	24.51750148949519	27.520816603496062	19.05997848050641
12	27.095986671308374	21.22936583202285	30.353163574055554	21.321483922613222
13	25.124293082108306	21.35819216035877	33.17931879367001	20.33819596386292
14	20.444782533702398	24.67690748576371	31.572306698905894	23.306003281628
15	18.03909774816127	25.92441893892343	34.71904298275597	21.317440330159325
16	20.718799103586097	26.67223081836625	30.279115287243542	22.329854790804113
17	20.733899394156126	25.713962587924442	29.98582847207173	23.566309545847705
18	20.545935526181946	24.68006654236832	32.5459279444461	22.22806998700364
19	21.201945220694853	26.32321824468915	30.736104415666144	21.738732118949855
20	23.25716426652076	26.767318422164955	31.01972451762785	18.955792793686435
21	24.654162278210535	26.82601369387857	29.543307822898235	18.976516205012665
22	22.03776841714205	24.68834327067239	29.985386204147087	23.28850210803847
23	20.983338503655975	25.82156005587739	29.671375977649046	23.523725462817588
24	22.68240550787837	23.44196907789033	29.65791839651341	24.217707017717885
25	20.12976140909088	26.61081875797267	27.84114494320332	25.418274889733127
26	20.20482059401637	27.394896607236387	28.351774852772166	24.04850794597508
27	20.08502916756963	27.77707927526187	29.002540513321424	23.135351043847074
28	17.65230285749306	27.835521822447117	31.178561883707545	23.333613436352273
29	19.548621356107184	25.35932689349114	30.000044226792465	25.09200752360921
30	21.23694756787391	26.136644361621002	30.77963621567764	21.84677185482745
31	22.156485764343223	28.269007569731436	27.2964604034368	22.27804626248854
32	23.05226785514588	27.493901441224804	26.76055804103109	22.693272662598226
33	21.220457292397857	26.2784860031679	29.444366170041906	23.05669053439233
34	21.928844145415166	24.961538485838897	29.305620403967524	23.803996964778413
35	21.76849043216526	24.52129235742072	28.349942599941496	25.360274610472526
36	21.436410401888864	28.01255535456935	29.26259405301276	21.288440190529023
37	20.206589665714947	29.302082260570362	29.0374164982363	21.45391157547839
38	18.90973374839125	28.15951466781572	28.834099615163723	24.096651968629303
39	21.272708088638073	23.70442350060117	31.366399389417538	23.65646902134322
40	24.163560787514903	22.760307843748006	30.952499793081795	22.1236315756553
41	23.145902292906463	24.547196621578504	28.614987449068106	23.69191363644692
42	22.869927107927907	24.825004059387737	28.541507792444925	23.76356104023943
43	21.245856107498906	24.62149763291889	29.94810933621271	24.1845369233695
44	20.590920492231565	23.840894745920234	28.780016566092836	26.788168195755365
45	22.18011550774569	22.61341171163373	27.723754399776084	27.48271838084449
46	23.381567915610226	24.258332485653145	29.15360660015378	23.20649299858285
47	20.207726926092604	24.677981565009276	31.643827740434215	23.470463768463894
48	19.64674165424631	24.37490167436318	30.957238377988705	25.021118293401805
49	21.572313017019102	22.09273600206223	32.72871095958871	23.60624002132995
50	22.36422532666225	21.3039195678916	30.185354487218774	26.146500618227375
51	21.462188303782465	20.829302903615286	28.71380273966025	28.994706052942
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	201.0
1	717.0
2	1233.0
3	3020.0
4	4807.0
5	3288.5
6	1770.0
7	1835.0
8	1900.0
9	2120.0
10	2340.0
11	2527.0
12	2714.0
13	2706.0
14	2698.0
15	2821.0
16	2944.0
17	3001.5
18	3059.0
19	3133.5
20	3208.0
21	3897.5
22	4587.0
23	6247.0
24	7907.0
25	9705.5
26	13698.0
27	15892.0
28	18963.5
29	22035.0
30	24671.0
31	27307.0
32	30871.0
33	34435.0
34	40142.0
35	45849.0
36	50802.5
37	55756.0
38	61506.5
39	67257.0
40	73729.0
41	80201.0
42	89192.0
43	98183.0
44	108665.5
45	119148.0
46	140815.0
47	162482.0
48	183367.0
49	204252.0
50	191963.0
51	179674.0
52	146813.0
53	113952.0
54	94464.0
55	74976.0
56	66316.0
57	57656.0
58	54152.5
59	50649.0
60	46373.0
61	42097.0
62	35952.5
63	29808.0
64	24576.0
65	19344.0
66	15926.5
67	12509.0
68	9759.5
69	7010.0
70	5706.0
71	4402.0
72	3817.0
73	3232.0
74	2506.0
75	1357.0
76	934.0
77	780.0
78	626.0
79	456.5
80	287.0
81	185.5
82	84.0
83	57.5
84	31.0
85	25.0
86	19.0
87	11.5
88	4.0
89	4.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1582751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.34146854543654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.34343570364946	20.43298736143908
2	13.66922008375687	8.29488422423917
3	5.645818511925464	5.139072743784894
4	2.9392687018906107	3.567269154600002
5	1.7613696072660592	2.6721270267875523
6	1.1289768036351904	2.055288850561477
7	0.8079551780424962	1.7160182634489283
8	0.5750936328153248	1.3959348296597607
9	0.45213694078663275	1.234664889039677
>10	5.219335283369006	37.598601923839375
>50	0.3639142263883051	6.7691098228411155
>100	0.08545713154621568	4.708183684389085
>500	0.00400909746416909	0.7922729313067702
>1k	0.0031650769453966496	1.5768632160069291
>5k	8.4402051877244E-4	2.0467210780562533
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	9195	0.5809505095874209	No Hit
TCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	7821	0.4941396340927916	No Hit
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	7564	0.4779020831451062	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	7389	0.466845385028978	No Hit
TCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	3062	0.19346062646619716	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	2558	0.16161733589174798	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2502	0.15807919249458693	No Hit
TGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	1947	0.12301366418343758	No Hit
GCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	1940	0.12257139625879246	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT	1868	0.11802235474815685	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCC	1714	0.10829246040596405	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.527245283686442E-4	0.0	0.0	0.5050699699447354	0.0
2	2.527245283686442E-4	0.0	0.0	2.181075860953492	0.0
3	2.527245283686442E-4	0.0	0.0	3.1684705932897845	0.0
4	2.527245283686442E-4	0.0	0.0	4.164773865251072	0.0
5	2.527245283686442E-4	0.0	0.0	5.8214463298396275	0.0
6	2.527245283686442E-4	0.0	0.0	6.774154620657324	0.0
7	2.527245283686442E-4	0.0	0.0	7.60024792276233	0.0
8	2.527245283686442E-4	0.0	0.0	8.987579221242003	0.0
9	2.527245283686442E-4	0.0	0.0	9.575542836491653	0.0
10	2.527245283686442E-4	0.0	0.0	11.045578236879964	0.0
11	2.527245283686442E-4	0.0	0.0	13.370454354475214	0.0
12	2.527245283686442E-4	0.0	0.0	15.815564166441847	0.0
13	2.527245283686442E-4	0.0	0.0	16.55794246852474	0.0
14	2.527245283686442E-4	0.0	0.0	16.798915306324243	0.0
15	2.527245283686442E-4	0.0	0.0	17.18362521963341	0.0
16	2.527245283686442E-4	0.0	0.0	18.03347462740507	0.0
17	3.1590566046080525E-4	0.0	0.0	19.321548367367956	0.0
18	3.7908679255296634E-4	0.0	0.0	20.620489262050697	0.0
19	3.7908679255296634E-4	0.0	0.0	21.56498400569641	0.0
20	3.7908679255296634E-4	0.0	0.0	22.831386617351686	0.0
21	3.7908679255296634E-4	0.0	0.0	24.457732138536006	0.0
22	3.7908679255296634E-4	0.0	0.0	26.201847289940112	0.0
23	4.422679246451274E-4	0.0	0.0	27.770887524316837	0.0
24	4.422679246451274E-4	0.0	0.0	28.920847309526263	0.0
25	4.422679246451274E-4	0.0	0.0	29.978752185277408	0.0
26	4.422679246451274E-4	0.0	0.0	30.900501721369945	0.0
27	4.422679246451274E-4	0.0	0.0	31.790723872548494	0.0
28	4.422679246451274E-4	0.0	0.0	32.76472420488125	0.0
29	4.422679246451274E-4	0.0	0.0	33.79179668817142	0.0
30	5.054490567372884E-4	0.0	0.0	34.86657092619117	0.0
31	5.686301888294495E-4	0.0	0.0	35.84625756041222	0.0
32	5.686301888294495E-4	0.0	0.0	36.736921979515415	0.0
33	5.686301888294495E-4	0.0	0.0	37.654754285418235	0.0
34	5.686301888294495E-4	0.0	0.0	38.55641222150547	0.0
35	5.686301888294495E-4	0.0	0.0	39.4556692745732	0.0
36	5.686301888294495E-4	0.0	0.0	40.362255338963614	0.0
37	5.686301888294495E-4	0.0	0.0	41.23977808259164	0.0
38	5.686301888294495E-4	0.0	0.0	42.09278654696791	0.0
39	5.686301888294495E-4	0.0	0.0	42.937897369832655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATA	20	7.034095E-4	45.0	6
ACGGCAT	20	7.034095E-4	45.0	41
TAGGCCG	20	7.034095E-4	45.0	42
ATCGCCC	40	6.8175723E-9	45.0	10
CGTTTTT	1680	0.0	40.044643	1
GGACGTA	45	1.9290383E-8	40.0	8
TTATCCG	40	3.4593177E-7	39.375	1
TTCTGCG	580	0.0	39.181038	1
TTTGGGA	16205	0.0	39.02962	4
TGGGCGA	2175	0.0	38.793102	6
TTTGTCG	455	0.0	38.076923	1
TTGGGAC	5685	0.0	38.07388	5
TACGGGA	280	0.0	37.767857	4
TGGGATG	4075	0.0	37.546013	6
GGGCGAT	3245	0.0	37.511555	7
CAACGTA	30	1.1401166E-4	37.499996	33
CGTTCCG	30	1.1401166E-4	37.499996	37
TGACACG	30	1.1401166E-4	37.499996	39
ACGGATA	30	1.1401166E-4	37.499996	16
TTGGGAT	8215	0.0	37.41327	5
>>END_MODULE