##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933967.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	557048
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.363085407361666	33.0	31.0	34.0	31.0	34.0
2	32.59738837586707	34.0	31.0	34.0	31.0	34.0
3	32.66786345162356	34.0	31.0	34.0	31.0	34.0
4	36.03622488546768	37.0	35.0	37.0	35.0	37.0
5	36.08793317631515	37.0	35.0	37.0	35.0	37.0
6	35.72853326822823	37.0	35.0	37.0	35.0	37.0
7	36.05185370021973	37.0	35.0	37.0	35.0	37.0
8	36.052214530884235	37.0	36.0	37.0	35.0	37.0
9	37.9268267725582	39.0	38.0	39.0	35.0	39.0
10	37.600345033103075	39.0	37.0	39.0	35.0	39.0
11	37.54715213051658	39.0	37.0	39.0	35.0	39.0
12	37.38817480719794	39.0	37.0	39.0	35.0	39.0
13	37.31829932070486	39.0	37.0	39.0	34.0	39.0
14	38.51419267280378	40.0	38.0	41.0	34.0	41.0
15	38.58612902299263	40.0	38.0	41.0	35.0	41.0
16	38.6630954603553	40.0	38.0	41.0	35.0	41.0
17	38.634392368341686	40.0	38.0	41.0	35.0	41.0
18	38.61332057560569	40.0	38.0	41.0	35.0	41.0
19	38.64300562967644	40.0	38.0	41.0	35.0	41.0
20	38.59688034065287	40.0	38.0	41.0	35.0	41.0
21	38.51017506570349	40.0	38.0	41.0	34.0	41.0
22	38.500658830118766	40.0	38.0	41.0	34.0	41.0
23	38.45280299004754	40.0	38.0	41.0	34.0	41.0
24	38.45036334391291	40.0	38.0	41.0	34.0	41.0
25	38.32435265901682	40.0	37.0	41.0	34.0	41.0
26	38.23045590326148	40.0	37.0	41.0	34.0	41.0
27	38.26069925751461	40.0	37.0	41.0	34.0	41.0
28	38.20264501443323	40.0	37.0	41.0	34.0	41.0
29	38.200625439818474	40.0	37.0	41.0	34.0	41.0
30	38.17547500394939	40.0	37.0	41.0	34.0	41.0
31	38.07270827648605	40.0	37.0	41.0	34.0	41.0
32	37.85087820080137	40.0	36.0	41.0	34.0	41.0
33	37.856732274418	40.0	37.0	41.0	34.0	41.0
34	37.86984963593802	40.0	37.0	41.0	34.0	41.0
35	37.75202675532449	40.0	36.0	41.0	33.0	41.0
36	37.69220067211443	40.0	36.0	41.0	33.0	41.0
37	37.66321394206604	40.0	36.0	41.0	33.0	41.0
38	37.58011338340682	40.0	36.0	41.0	33.0	41.0
39	37.623280220016944	40.0	36.0	41.0	33.0	41.0
40	37.51609735606267	40.0	36.0	41.0	33.0	41.0
41	37.32952277003059	39.0	35.0	41.0	33.0	41.0
42	37.27206811621261	39.0	35.0	41.0	33.0	41.0
43	37.26114984705088	39.0	35.0	41.0	33.0	41.0
44	37.214098246470684	39.0	35.0	41.0	32.0	41.0
45	37.250220806824544	39.0	35.0	41.0	33.0	41.0
46	37.15968282805073	39.0	35.0	41.0	32.0	41.0
47	37.05876692852321	39.0	35.0	41.0	32.0	41.0
48	37.00460283494421	39.0	35.0	41.0	32.0	41.0
49	37.013006060519025	39.0	35.0	41.0	32.0	41.0
50	36.91897466645603	39.0	35.0	41.0	32.0	41.0
51	35.932059714782206	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	10.0
11	8.0
12	14.0
13	9.0
14	15.0
15	6.0
16	18.0
17	25.0
18	58.0
19	102.0
20	187.0
21	329.0
22	523.0
23	751.0
24	1152.0
25	1758.0
26	2471.0
27	3012.0
28	3657.0
29	4550.0
30	5976.0
31	7810.0
32	10751.0
33	15541.0
34	29664.0
35	42791.0
36	39215.0
37	63189.0
38	126727.0
39	196594.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.23767431172897	4.730472059858396	55.26022892102655	16.771624707386078
2	18.39697835734084	4.9713848716807165	54.812870704140394	21.818766066838048
3	21.232102080969682	4.629762605735951	52.350246298344125	21.787889014950238
4	19.746772270971263	4.269650012207206	52.25958983785958	23.723987878961957
5	18.912194281282762	5.102791859947438	51.44080940960204	24.544204449167754
6	20.377777139492466	6.657415518949892	53.46307679050998	19.501730551047665
7	81.16463931294969	1.9319699559104422	11.170671109132426	5.73271962200744
8	81.83262483663886	3.106554551851905	8.60392641208657	6.4568941994226705
9	76.7090089184415	4.9437391391765155	11.577637833723484	6.769614108658501
10	36.08145079059614	26.537569473366744	19.581256911433126	17.799722824603982
11	26.27529405006391	28.268838592006436	25.838527380046244	19.617339977883415
12	25.05511194726487	22.80772931596559	31.35833895822263	20.77881977854691
13	22.8789978601485	24.420696241616522	32.405465956255114	20.294839941979863
14	17.979061050394222	27.73243957432753	31.458330341370942	22.83016903390731
15	16.16772701813847	26.119472648676595	36.7201749220893	20.992625411095634
16	17.90276600939237	26.021994513937756	32.51640792175899	23.558831554910885
17	18.088387356206287	26.40346971894702	30.17495799284801	25.33318493199868
18	18.89603768436472	24.373303557323606	32.77527250793468	23.955386250376986
19	21.15096006089242	25.642673521850902	30.70256064109377	22.503805776162917
20	24.119644985710387	25.229244158492627	31.4836423432092	19.167468512587785
21	22.799471499763037	26.454273240367076	30.484087547213168	20.26216771265672
22	20.100242707989256	25.11668653329695	29.84913328833422	24.933937470379572
23	20.15373899556232	26.145143686001926	28.777771394924674	24.923345923511082
24	21.90224181758125	23.306250089758873	29.720419066220504	25.071089026439374
25	18.39320848472663	27.97784032973819	27.795270784564348	25.83368040097083
26	18.72441872154644	28.997321595266477	28.47025750025132	23.808002182935763
27	20.309021843719034	27.645193950970114	28.747432896267465	23.298351309043387
28	16.932293087848805	26.870754405365428	31.157279085464808	25.039673421320963
29	19.16531429966538	27.00072525168388	29.15529720957619	24.67866323907455
30	23.600300153667188	26.343331274863207	28.216419410894574	21.83994916057503
31	22.698582527897056	28.28714940184688	25.952341629446657	23.061926440809412
32	22.46072151771481	28.761614798006637	27.05476009248754	21.722903591791013
33	23.281297123407676	25.133740718932657	28.383191394637446	23.201770763022218
34	20.620305611006593	25.136792520572733	29.33032700952162	24.912574858899053
35	20.856191926009966	25.36926799844897	28.923001249443498	24.85153882609757
36	24.04227283824733	25.729560109721245	28.82103517111631	21.40713188091511
37	22.246556849679024	25.541784549984918	29.568188019703868	22.64347058063219
38	19.994147721560797	26.911145897660525	29.278625899383897	23.81608048139478
39	22.43792276428602	25.604436242478208	30.06760638221482	21.890034611020955
40	25.564762821157245	22.95924229150809	29.87354770145481	21.602447185879853
41	20.90914966035243	24.033296951070643	29.898141632318936	25.159411756257988
42	20.702165702057993	23.614302537662823	30.78280507245336	24.900726687825824
43	22.30795191796757	23.732604730651577	29.795457483017625	24.16398586836323
44	20.934820697677758	24.04837644152748	27.84176588013959	27.17503698065517
45	21.896317732044636	24.202402665479454	26.579576625353653	27.321702977122257
46	23.76204564059112	23.83600695092703	29.70013356120119	22.701813847280665
47	19.325623644641034	24.135622064884892	32.81871580186986	23.720038488604214
48	19.67765793971076	22.730177650759	32.245336129022995	25.346828280507243
49	21.87262138989818	20.74489092501903	33.22119458287257	24.16129310221022
50	22.898924329680746	20.855653372779365	30.491986327928654	25.75343596961124
51	20.66733925981244	20.562859933075785	28.713145007252518	30.056655799859257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	269.5
2	419.0
3	1151.0
4	1883.0
5	1241.0
6	599.0
7	641.0
8	683.0
9	772.5
10	862.0
11	862.0
12	862.0
13	914.0
14	966.0
15	929.5
16	893.0
17	924.5
18	956.0
19	1030.5
20	1105.0
21	1441.0
22	1777.0
23	1957.0
24	2137.0
25	2790.5
26	4500.5
27	5557.0
28	6117.0
29	6677.0
30	7740.0
31	8803.0
32	9683.5
33	10564.0
34	12929.5
35	15295.0
36	16328.5
37	17362.0
38	19129.0
39	20896.0
40	23535.0
41	26174.0
42	30701.5
43	35229.0
44	39423.0
45	43617.0
46	51813.0
47	60009.0
48	67809.5
49	75610.0
50	71533.0
51	67456.0
52	55399.0
53	43342.0
54	36120.5
55	28899.0
56	25452.0
57	22005.0
58	18720.0
59	15435.0
60	14471.5
61	13508.0
62	11895.5
63	10283.0
64	7642.5
65	5002.0
66	4218.0
67	3434.0
68	2908.0
69	2382.0
70	1946.5
71	1511.0
72	1019.5
73	528.0
74	458.0
75	284.5
76	181.0
77	128.5
78	76.0
79	80.0
80	84.0
81	53.5
82	23.0
83	14.0
84	5.0
85	4.5
86	4.0
87	3.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	557048.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.32131485087365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.71042341359106	18.61005590480532
2	13.841983329284144	7.840463360584011
3	6.122930223575924	5.202283040154718
4	3.208539230834163	3.6348019907133717
5	1.9289251388801234	2.731484809099458
6	1.190138827827615	2.0223777875493356
7	0.8533383768966653	1.6917365392496762
8	0.6230234239604896	1.4115874039563494
9	0.5026632988642419	1.2812476996001694
>10	5.3763387502450595	37.40500783991176
>50	0.545537746374139	9.449221179669694
>100	0.08718347063088473	4.421050319835083
>500	0.005128439448875572	1.1011321994907715
>1k	0.003846329586656679	3.1975499253802915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	4482	0.8045985265183612	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT	4275	0.7674383536068705	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	3504	0.6290301733423331	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	2978	0.5346038402435697	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG	1267	0.22744898105728767	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1106	0.19854662434835058	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG	921	0.16533584179460298	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	879	0.1577960965661846	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	869	0.15600091913084688	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	812	0.14576840774942196	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG	725	0.13015036406198388	No Hit
TGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG	678	0.12171303011589665	TruSeq Adapter, Index 21 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT	640	0.11489135586161336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7951774353377087E-4	0.0	0.0	0.8685068432163835	0.0
2	1.7951774353377087E-4	0.0	0.0	3.1577171087590297	0.0
3	1.7951774353377087E-4	0.0	0.0	4.359588401717626	0.0
4	1.7951774353377087E-4	0.0	0.0	5.5388404589909666	0.0
5	1.7951774353377087E-4	0.0	0.0	7.456628513162241	0.0
6	1.7951774353377087E-4	0.0	0.0	8.519014519395096	0.0
7	1.7951774353377087E-4	0.0	0.0	9.543522281742327	0.0
8	1.7951774353377087E-4	0.0	0.0	11.127407332940788	0.0
9	1.7951774353377087E-4	0.0	0.0	11.827167497235427	0.0
10	1.7951774353377087E-4	0.0	0.0	13.37209719808706	0.0
11	1.7951774353377087E-4	0.0	0.0	15.898809438324884	0.0
12	1.7951774353377087E-4	0.0	0.0	18.394824144418433	0.0
13	1.7951774353377087E-4	0.0	0.0	19.153286610848617	0.0
14	1.7951774353377087E-4	0.0	0.0	19.398328330772213	0.0
15	1.7951774353377087E-4	0.0	0.0	19.812116729617557	0.0
16	1.7951774353377087E-4	0.0	0.0	20.803593227154572	0.0
17	1.7951774353377087E-4	0.0	0.0	22.237042409271734	0.0
18	1.7951774353377087E-4	0.0	0.0	23.82703106375034	0.0
19	1.7951774353377087E-4	0.0	0.0	24.850820755123436	0.0
20	1.7951774353377087E-4	0.0	0.0	25.961317516623343	0.0
21	1.7951774353377087E-4	0.0	0.0	27.42941362324252	0.0
22	1.7951774353377087E-4	0.0	0.0	28.83611466157315	0.0
23	1.7951774353377087E-4	0.0	0.0	30.186088092947106	0.0
24	1.7951774353377087E-4	0.0	0.0	31.208262124628398	0.0
25	1.7951774353377087E-4	0.0	0.0	32.06527983225862	0.0
26	1.7951774353377087E-4	0.0	0.0	32.81297123407678	0.0
27	1.7951774353377087E-4	0.0	0.0	33.62349384613175	0.0
28	1.7951774353377087E-4	0.0	0.0	34.43078513880312	0.0
29	1.7951774353377087E-4	0.0	0.0	35.26446553977395	0.0
30	1.7951774353377087E-4	0.0	0.0	36.21501199178527	0.0
31	1.7951774353377087E-4	0.0	0.0	37.09213568669127	0.0
32	1.7951774353377087E-4	0.0	0.0	37.88847639700708	0.0
33	1.7951774353377087E-4	0.0	0.0	38.64083526015711	0.0
34	1.7951774353377087E-4	0.0	0.0	39.42550731714323	0.0
35	1.7951774353377087E-4	0.0	0.0	40.2631371084718	0.0
36	1.7951774353377087E-4	0.0	0.0	41.03596099438469	0.0
37	1.7951774353377087E-4	0.0	0.0	41.77377892030848	0.0
38	1.7951774353377087E-4	0.0	0.0	42.53619077709641	0.0
39	1.7951774353377087E-4	0.0	0.0	43.28729301604171	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGCGC	20	7.03015E-4	45.000004	2
GCGAATA	20	7.03015E-4	45.000004	9
GCGAAGT	20	7.03015E-4	45.000004	11
GCGCGAC	70	0.0	45.000004	9
TGGTTAG	40	6.8048394E-9	45.000004	1
ACGATGC	20	7.03015E-4	45.000004	38
TCGTGTA	20	7.03015E-4	45.000004	30
AACATCG	20	7.03015E-4	45.000004	25
AGGCACC	20	7.03015E-4	45.000004	6
TGCGAAG	20	7.03015E-4	45.000004	10
TACTCGA	80	0.0	45.000004	23
CGTCGTA	20	7.03015E-4	45.000004	18
TTCGGCA	20	7.03015E-4	45.000004	1
TTACGCG	20	7.03015E-4	45.000004	1
CTTTCGT	20	7.03015E-4	45.000004	15
CCGTTCA	20	7.03015E-4	45.000004	29
CCCATCG	20	7.03015E-4	45.000004	22
CTGGACG	80	0.0	45.000004	26
GGTAGCG	20	7.03015E-4	45.000004	1
AGCGTTG	25	3.8882077E-5	45.0	16
>>END_MODULE