##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933966.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012538
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4855165929575	34.0	31.0	34.0	31.0	34.0
2	32.71116837096484	34.0	31.0	34.0	31.0	34.0
3	32.7625570595869	34.0	31.0	34.0	31.0	34.0
4	36.11712745595721	37.0	35.0	37.0	35.0	37.0
5	36.15606722908177	37.0	35.0	37.0	35.0	37.0
6	35.787652414032856	37.0	35.0	37.0	35.0	37.0
7	36.115910711499225	37.0	35.0	37.0	35.0	37.0
8	36.13281081796436	37.0	37.0	37.0	35.0	37.0
9	37.98050147253733	39.0	39.0	39.0	35.0	39.0
10	37.64902551805463	39.0	37.0	39.0	35.0	39.0
11	37.58849445650435	39.0	37.0	39.0	35.0	39.0
12	37.42407791115	39.0	37.0	39.0	35.0	39.0
13	37.386669932387726	39.0	37.0	39.0	35.0	39.0
14	38.59107213753953	40.0	38.0	41.0	35.0	41.0
15	38.652328110154876	40.0	38.0	41.0	35.0	41.0
16	38.73002000912558	40.0	38.0	41.0	35.0	41.0
17	38.697923435959936	40.0	38.0	41.0	35.0	41.0
18	38.646771775479046	40.0	38.0	41.0	35.0	41.0
19	38.667444579857744	40.0	38.0	41.0	35.0	41.0
20	38.62702930655442	40.0	38.0	41.0	35.0	41.0
21	38.53250347147465	40.0	38.0	41.0	34.0	41.0
22	38.54741550440576	40.0	38.0	41.0	35.0	41.0
23	38.499751120451776	40.0	38.0	41.0	34.0	41.0
24	38.47507254048737	40.0	38.0	41.0	34.0	41.0
25	38.37511481050588	40.0	37.0	41.0	34.0	41.0
26	38.311506333589456	40.0	37.0	41.0	34.0	41.0
27	38.285303860200806	40.0	37.0	41.0	34.0	41.0
28	38.17728223533339	40.0	37.0	41.0	34.0	41.0
29	38.1665201701072	40.0	37.0	41.0	34.0	41.0
30	38.12082015687312	40.0	37.0	41.0	34.0	41.0
31	38.024269706420895	40.0	37.0	41.0	34.0	41.0
32	37.953644208908706	40.0	37.0	41.0	34.0	41.0
33	37.89081693724087	40.0	37.0	41.0	33.0	41.0
34	37.82148225548078	40.0	37.0	41.0	33.0	41.0
35	37.798496451491204	40.0	37.0	41.0	33.0	41.0
36	37.69412110952873	40.0	36.0	41.0	33.0	41.0
37	37.57448510574418	40.0	36.0	41.0	33.0	41.0
38	37.504334652131575	40.0	36.0	41.0	33.0	41.0
39	37.51538213874442	40.0	36.0	41.0	33.0	41.0
40	37.4288451396392	40.0	36.0	41.0	33.0	41.0
41	37.309174569250736	40.0	35.0	41.0	33.0	41.0
42	37.2885047277238	40.0	35.0	41.0	33.0	41.0
43	37.16866132431573	39.0	35.0	41.0	32.0	41.0
44	37.03880940764692	39.0	35.0	41.0	32.0	41.0
45	37.020883166853984	39.0	35.0	41.0	32.0	41.0
46	36.94275572867389	39.0	35.0	41.0	32.0	41.0
47	36.849030851187806	39.0	35.0	41.0	32.0	41.0
48	36.792051261286	39.0	35.0	41.0	31.0	41.0
49	36.76138080743636	39.0	35.0	41.0	31.0	41.0
50	36.66693003126796	39.0	35.0	41.0	31.0	41.0
51	35.68457973922954	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	18.0
11	16.0
12	17.0
13	10.0
14	15.0
15	17.0
16	30.0
17	85.0
18	115.0
19	249.0
20	390.0
21	706.0
22	1047.0
23	1655.0
24	2455.0
25	3707.0
26	4984.0
27	6445.0
28	7392.0
29	9010.0
30	11227.0
31	14663.0
32	19344.0
33	28148.0
34	54624.0
35	69003.0
36	70782.0
37	114094.0
38	219868.0
39	372157.0
40	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.660186580651786	4.508176483252974	54.21919967448135	18.612437261613884
2	18.816083939565726	4.429167102864287	54.80772079665158	21.947028160918407
3	20.209611886171185	4.584321773602571	52.86576898842314	22.34029735180309
4	19.226241385508494	4.533459484977354	51.69099826376886	24.549300865745284
5	18.775591632116523	5.588135951440835	50.12542739136704	25.5108450250756
6	21.9051531893124	6.3394163972117585	52.11359968712286	19.641830726352985
7	84.0310190827406	2.2287558590393646	8.986823210585678	4.75340184763436
8	85.44706470275683	2.6782204717255054	6.945122059616528	4.929592765901131
9	79.72727937124336	5.375699479920754	9.404190262488914	5.492830886346982
10	36.7183256332108	34.39841270154799	15.424606286381351	13.458655378859854
11	27.88231157744203	25.18512885442324	27.076712182653885	19.85584738548084
12	26.776674060627847	22.189883243888133	30.853459326958593	20.179983368525427
13	24.590188220096433	22.68151911335673	30.916074260916627	21.812218405630208
14	19.971596127750267	25.21446108689254	31.231519212118457	23.582423573238735
15	17.748765972240054	26.0479112882677	34.01650110909418	22.186821630398068
16	21.395838970981828	25.422255757314787	31.509829754537606	21.672075517165776
17	21.417566550588717	25.618890352757127	29.03367577315617	23.929867323497984
18	22.298718665373546	24.465847207709736	30.563297377481142	22.672136749435577
19	23.15705682157114	25.976111513839477	28.734822791835963	22.132008872753417
20	26.170968398223078	25.930977405292445	29.416673744590327	18.48138045189415
21	25.93532292121382	25.59775534350316	29.35929318208304	19.10762855319998
22	23.695406987194552	23.645729839275166	29.74782181014441	22.911041363385866
23	22.83084684229135	24.566189120803365	29.13085731103425	23.47210672587103
24	23.417590253402835	23.130094870513503	29.767870440418037	23.684444435665625
25	22.559449620656213	26.15161110002785	26.796920214352447	24.49201906496349
26	22.978989430520137	26.44918017891674	27.26524831660639	23.30658207395673
27	22.724579225668567	26.35130730896026	28.991306992922734	21.93280647244844
28	19.67491590439075	26.123957816891814	30.951628482091536	23.249497796625903
29	21.926485722017347	23.95110109447744	30.78373354876558	23.338679634739634
30	24.621594448800934	23.350333518346964	30.278271037728956	21.749800995123145
31	22.862450594446827	25.667678645147145	29.15120222648434	22.31866853392169
32	23.413837307834374	25.085181988231554	28.51270767121827	22.98827303271581
33	23.775996555191014	24.2768172651298	29.182114646561413	22.76507153311777
34	21.479391390742865	23.983988749064235	31.56582765288809	22.970792207304815
35	22.66966770629843	23.809378018405237	28.941728606728834	24.5792256685675
36	24.69892487985636	24.449156476102626	28.304419192168588	22.547499451872426
37	22.202623506475806	25.28221163057584	31.339959586701934	21.175205276246423
38	20.897586065905674	25.417021385864036	30.59381475065627	23.09157779757402
39	22.810699450292233	23.204264926353382	30.915679214014684	23.0693564093397
40	24.36530777116513	22.373382529840853	30.89316154060391	22.368148158390106
41	20.329607382636503	23.602472203512363	30.411698128860348	25.656222284990786
42	22.278077464747	23.255225976704082	29.595333705994243	24.871362852554668
43	22.94649682283529	22.841908155545767	30.333182557099093	23.878412464519847
44	22.52991986473594	22.81919295868402	30.01299704307394	24.6378901335061
45	23.420651866892896	21.723629137869395	28.274296865895405	26.581422129342307
46	23.018889167616425	22.771787330450806	30.220100381417787	23.989223120514982
47	20.33444670718531	23.456798658420723	32.82424955902889	23.384505075365073
48	21.05886396362408	22.22188204294555	32.464954401711346	24.25429959171903
49	22.422368345681843	21.52600692517219	32.86503815165456	23.186586577491415
50	21.63760767497121	21.76451649222054	30.980170620756947	25.617705212051302
51	19.94364655943777	21.073678222446958	29.734884024105764	29.24779119400951
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	247.0
1	699.5
2	1152.0
3	2249.5
4	3347.0
5	2376.0
6	1405.0
7	1438.5
8	1472.0
9	1566.5
10	1661.0
11	1648.5
12	1636.0
13	1664.5
14	1693.0
15	1615.0
16	1537.0
17	1883.5
18	2230.0
19	2210.0
20	2190.0
21	2637.5
22	3085.0
23	3920.5
24	4756.0
25	5554.5
26	8262.5
27	10172.0
28	11925.0
29	13678.0
30	15746.5
31	17815.0
32	20727.5
33	23640.0
34	26320.0
35	29000.0
36	31311.5
37	33623.0
38	36234.5
39	38846.0
40	42530.5
41	46215.0
42	51114.5
43	56014.0
44	60838.5
45	65663.0
46	75369.0
47	85075.0
48	96560.0
49	108045.0
50	106636.5
51	105228.0
52	90533.5
53	75839.0
54	67151.5
55	58464.0
56	53583.5
57	48703.0
58	46545.5
59	44388.0
60	42558.5
61	40729.0
62	34651.0
63	28573.0
64	23771.5
65	18970.0
66	15568.5
67	12167.0
68	9943.0
69	7719.0
70	6181.5
71	4644.0
72	3766.0
73	2888.0
74	2417.0
75	1493.0
76	1040.0
77	677.0
78	314.0
79	245.5
80	177.0
81	138.0
82	99.0
83	89.5
84	80.0
85	46.5
86	13.0
87	8.0
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1012538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.767968571272874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.29496434654597	19.934753564689085
2	12.483375655896598	7.182427170644525
3	5.001672028169482	4.316638311305828
4	2.3680586990984858	2.7249695292237823
5	1.3768774592556225	1.9804983737179898
6	0.8925054155064163	1.540534064578765
7	0.6611405733703237	1.331376986513756
8	0.49154116584212254	1.1312512648346404
9	0.3871309813378343	1.0023274713683559
>10	6.647958003073977	47.02104427021379
>50	0.32170758801366195	5.704946695583363
>100	0.06856625881573149	3.3808656070603487
>500	0.0020777654186574027	0.44909515150493556
>1k	0.0017314711822145024	1.1311061113990768
>5k	6.925884728858009E-4	1.1681654273617734
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	6683	0.6600246114219911	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	5043	0.4980553816251835	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	3593	0.3548508796706889	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	3385	0.3343084407696304	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1655	0.1634506556790955	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTG	1433	0.14152555262123495	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTG	1288	0.1272051024257855	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.87617254858583E-5	0.0	0.0	0.5229433364476197	0.0
2	9.87617254858583E-5	0.0	0.0	1.9710865172467602	0.0
3	9.87617254858583E-5	0.0	0.0	2.7180214470963064	0.0
4	9.87617254858583E-5	0.0	0.0	3.4442164145938228	0.0
5	1.975234509717166E-4	0.0	0.0	4.62106113548331	0.0
6	1.975234509717166E-4	0.0	0.0	5.288591638042226	0.0
7	1.975234509717166E-4	0.0	0.0	5.914642215897082	0.0
8	1.975234509717166E-4	0.0	0.0	6.8874452119327865	0.0
9	1.975234509717166E-4	0.0	0.0	7.338094965324758	0.0
10	1.975234509717166E-4	0.0	0.0	8.220037173913473	0.0
11	1.975234509717166E-4	0.0	0.0	9.974045418542316	0.0
12	1.975234509717166E-4	0.0	0.0	11.650031900037332	0.0
13	1.975234509717166E-4	0.0	0.0	12.182061315229651	0.0
14	1.975234509717166E-4	0.0	0.0	12.386004278357948	0.0
15	1.975234509717166E-4	0.0	0.0	12.693350768069939	0.0
16	1.975234509717166E-4	0.0	0.0	13.376189338079163	0.0
17	1.975234509717166E-4	0.0	0.0	14.307512409410807	0.0
18	1.975234509717166E-4	0.0	0.0	15.269056568741124	0.0
19	1.975234509717166E-4	0.0	0.0	16.00502894706174	0.0
20	1.975234509717166E-4	0.0	0.0	16.819220611967157	0.0
21	1.975234509717166E-4	0.0	0.0	17.9195249956051	0.0
22	1.975234509717166E-4	0.0	0.0	19.0928142943771	0.0
23	1.975234509717166E-4	0.0	0.0	20.12438051707689	0.0
24	1.975234509717166E-4	0.0	0.0	20.959114620883366	0.0
25	1.975234509717166E-4	0.0	0.0	21.64926155857854	0.0
26	1.975234509717166E-4	0.0	0.0	22.296348383961885	0.0
27	1.975234509717166E-4	0.0	0.0	22.926349430836176	0.0
28	1.975234509717166E-4	0.0	0.0	23.566621697160997	0.0
29	1.975234509717166E-4	0.0	0.0	24.273163081286825	0.0
30	1.975234509717166E-4	0.0	0.0	25.066812307291183	0.0
31	1.975234509717166E-4	0.0	0.0	25.754095154947272	0.0
32	1.975234509717166E-4	0.0	0.0	26.45550092934784	0.0
33	1.975234509717166E-4	0.0	0.0	27.101106328848893	0.0
34	1.975234509717166E-4	0.0	0.0	27.793228501053786	0.0
35	1.975234509717166E-4	0.0	0.0	28.469845082357402	0.0
36	1.975234509717166E-4	0.0	0.0	29.142017386014153	0.0
37	1.975234509717166E-4	0.0	0.0	29.825646049827267	0.0
38	1.975234509717166E-4	0.0	0.0	30.522706308306454	0.0
39	1.975234509717166E-4	0.0	0.0	31.3478605247408	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATA	20	7.032888E-4	45.0	14
CGTTGAC	25	3.890475E-5	45.0	31
ACGATAA	20	7.032888E-4	45.0	45
GCGCTAA	20	7.032888E-4	45.0	45
TACGGGA	190	0.0	42.63158	4
TCTACGG	70	0.0	41.785713	2
GCGCGAC	60	3.6379788E-12	41.250004	9
TTCGTTG	300	0.0	41.250004	1
TTCGACG	55	6.184564E-11	40.909092	25
TACGGAT	45	1.9279469E-8	40.0	43
CGTTTCG	45	1.9279469E-8	40.0	32
CATACGA	210	0.0	39.642857	18
TGCGTAT	40	3.4576806E-7	39.375	16
TCGGGTC	75	0.0	39.000004	5
CGCTTGG	325	0.0	38.76923	2
TCTAGCG	35	6.2475538E-6	38.571426	1
GCATCCG	35	6.2475538E-6	38.571426	9
ACACGCG	440	0.0	38.352272	36
CTTTGCG	405	0.0	38.333332	1
CGGTAGG	270	0.0	38.333332	31
>>END_MODULE