FastQCFastQC Report
Sat 14 Jan 2017
SRR2933965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186471
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT120301.0139312296718588TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC120051.0118241406658905TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC88590.7466680601548625TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC66470.5602328249068035TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG30830.2598462162159884TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG30520.257233425848588TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.2046404842596237No Hit
GCCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG19090.16089731649572556No Hit
TTCCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT19000.16013876445357703No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCC17580.14817049889967812No Hit
CGTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTC17230.14522057429132276No Hit
TGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG16750.14117496339986396TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGC16610.1399949935565218No Hit
GAATCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTC15180.12794244444238417No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCG14810.12482395271355136No Hit
TTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG14790.12465538559307392TruSeq Adapter, Index 21 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT12430.10476446537673488No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT207.033378E-445.0000044
GGCGTAA207.033378E-445.0000048
TCTAGCG453.8562575E-1045.0000041
AAAGTCG207.033378E-445.00000432
GTGCTCG302.1655615E-645.0000041
CGACTTG207.033378E-445.00000418
CCGTTGT207.033378E-445.00000429
GCGTAAG207.033378E-445.00000444
ACAACGA253.8908827E-545.027
CGTATAA351.2119744E-745.028
GACCGAC253.8908827E-545.014
TTATCCG253.8908827E-545.01
TTGTTCG2800.044.1964261
TCGATTG950.042.631581
TCTCGCG950.042.631581
GCGCGAC1200.041.2500049
CGAATAG1200.041.25000426
AGTGCGA501.0822987E-940.510
CGTTTTT16300.040.0306741
TACGCGT451.9283107E-840.00000424
Produced by FastQC (version 0.11.5)