##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933965.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1186471
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.296115117857916	33.0	31.0	34.0	31.0	34.0
2	32.5352360066112	34.0	31.0	34.0	31.0	34.0
3	32.589594688787166	34.0	31.0	34.0	31.0	34.0
4	35.98929935919209	37.0	35.0	37.0	35.0	37.0
5	36.040748572868615	37.0	35.0	37.0	35.0	37.0
6	35.67848940260655	37.0	35.0	37.0	35.0	37.0
7	36.010726768711585	37.0	35.0	37.0	35.0	37.0
8	35.999222062738994	37.0	35.0	37.0	35.0	37.0
9	37.83397234319254	39.0	38.0	39.0	35.0	39.0
10	37.50421122808733	39.0	37.0	39.0	35.0	39.0
11	37.48447538962183	39.0	37.0	39.0	35.0	39.0
12	37.32385283753248	39.0	37.0	39.0	35.0	39.0
13	37.23301454481399	39.0	37.0	39.0	34.0	39.0
14	38.36172734099696	40.0	38.0	41.0	34.0	41.0
15	38.39448330384813	40.0	38.0	41.0	34.0	41.0
16	38.46848679824454	40.0	38.0	41.0	34.0	41.0
17	38.43974610420314	40.0	38.0	41.0	34.0	41.0
18	38.39113387516425	40.0	38.0	41.0	34.0	41.0
19	38.41964110374379	40.0	38.0	41.0	34.0	41.0
20	38.392682164165834	40.0	38.0	41.0	34.0	41.0
21	38.31560737683433	40.0	37.0	41.0	34.0	41.0
22	38.317095824508144	40.0	37.0	41.0	34.0	41.0
23	38.28027655121785	40.0	37.0	41.0	34.0	41.0
24	38.26524879242729	40.0	37.0	41.0	34.0	41.0
25	38.14798338939595	40.0	37.0	41.0	34.0	41.0
26	38.08687359404486	40.0	37.0	41.0	34.0	41.0
27	38.102847014381304	40.0	37.0	41.0	34.0	41.0
28	38.01443524536209	40.0	37.0	41.0	34.0	41.0
29	38.02376543548051	40.0	37.0	41.0	34.0	41.0
30	37.9829131938328	40.0	37.0	41.0	34.0	41.0
31	37.83362340925316	40.0	36.0	41.0	33.0	41.0
32	37.660851381955396	40.0	36.0	41.0	33.0	41.0
33	37.63686259504025	40.0	36.0	41.0	33.0	41.0
34	37.58568308875649	40.0	36.0	41.0	33.0	41.0
35	37.49756968354052	40.0	36.0	41.0	33.0	41.0
36	37.434644420301886	39.0	36.0	41.0	33.0	41.0
37	37.390586032022696	39.0	36.0	41.0	33.0	41.0
38	37.35866700492469	39.0	35.0	41.0	33.0	41.0
39	37.42192097404825	39.0	36.0	41.0	33.0	41.0
40	37.315976538828174	39.0	35.0	41.0	33.0	41.0
41	37.12397858860436	39.0	35.0	41.0	32.0	41.0
42	37.05755387194461	39.0	35.0	41.0	32.0	41.0
43	37.01749052442074	39.0	35.0	41.0	32.0	41.0
44	36.96076768838008	39.0	35.0	41.0	31.0	41.0
45	37.05069150446998	39.0	35.0	41.0	32.0	41.0
46	36.98503545387961	39.0	35.0	41.0	32.0	41.0
47	36.885059980395646	39.0	35.0	41.0	31.0	41.0
48	36.84481120903924	39.0	35.0	41.0	31.0	41.0
49	36.8755114958562	39.0	35.0	41.0	32.0	41.0
50	36.80830884193546	39.0	35.0	41.0	31.0	41.0
51	35.76706889591065	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	22.0
10	36.0
11	17.0
12	18.0
13	11.0
14	25.0
15	25.0
16	45.0
17	85.0
18	146.0
19	263.0
20	525.0
21	782.0
22	1279.0
23	1812.0
24	2737.0
25	4172.0
26	5852.0
27	7323.0
28	8834.0
29	11091.0
30	14207.0
31	18967.0
32	25628.0
33	37092.0
34	69410.0
35	84331.0
36	87610.0
37	142985.0
38	285510.0
39	375457.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.824553655335865	5.228867793650245	55.114537144186414	17.83204140682747
2	19.78986422761281	4.829279434558451	52.74043781938202	22.640418518446722
3	20.679308638812074	5.209061156994145	51.59106290840653	22.520567295787256
4	19.826021874955224	4.873444020123543	51.61634797647814	23.684186128443088
5	18.624475440191965	5.50877349720305	49.7042068453422	26.16254421726279
6	21.75839106054847	6.51596204205581	52.3212113907546	19.404435506641125
7	78.7214352478906	2.4276193855559893	12.266629357144001	6.584316009409416
8	78.64338867110953	3.55904189820063	10.290854138027814	7.506715292662021
9	72.0827563421272	6.026611691309776	13.61820052913219	8.272431437430834
10	34.04069715989687	30.93855644175037	20.132055482182036	14.888690916170727
11	28.40507690453454	24.300383237348406	27.30011942980486	19.994420428312196
12	27.43219176869894	20.71588770395568	31.371015389335266	20.480905138010115
13	25.012663604925866	21.88152934205724	32.48692972689598	20.618877326120906
14	20.38145053692842	25.260204421347005	30.847277346011833	23.511067695712747
15	17.229076816879637	25.751661861099006	35.39471255513199	21.624548766889372
16	20.023582540154795	24.735539258860943	31.801451531474434	23.43942666950983
17	19.464192550850377	25.666535465257894	29.844134412050526	25.0251375718412
18	19.40856540109282	24.838618053032903	32.43222969630104	23.32058684957323
19	20.64331955859014	26.11652539337245	31.091193969342694	22.148961078694718
20	23.448950711816806	26.777392789204285	31.07475867509615	18.698897823882756
21	23.60807807354752	26.788855353396755	30.07743130679132	19.52563526626441
22	20.24811394463076	25.842098121235157	29.380743397858016	24.529044536276064
23	20.436319134643828	25.258181615901275	29.57434273572637	24.73115651372853
24	22.243948651083763	22.73380470319123	29.363549551569317	25.65869709415569
25	19.558421571197275	26.629980842346757	26.593317493642914	27.218280092813057
26	17.88792140726575	28.344561308283133	27.626043957248008	26.14147332720311
27	18.331084367000962	27.563758406231585	28.4461229983708	25.65903422839665
28	15.40214636514504	27.60016890425472	31.265913789717576	25.73177094088267
29	18.44124298023298	25.75857311303858	30.76906220211029	25.031121704618148
30	23.159183831716074	25.417646111872944	28.08109089897688	23.342079157434107
31	22.227176222596253	28.485399137442048	26.766773060614206	22.520651579347494
32	21.784434680662233	27.769410293214076	28.32762031267515	22.118534713448536
33	24.604394039129485	25.018310603461863	26.993242986975662	23.38405237043299
34	21.824722222456344	24.871994342887437	29.35099130109375	23.952292133562473
35	19.886958889007822	24.968836153601732	29.028438116060144	26.115766841330295
36	21.8011228255895	27.94109590542036	26.787253965752218	23.47052730323792
37	20.03850073031705	28.249742303014568	29.352508405178046	22.359248561490336
38	20.118148694742644	27.409098073193526	30.389786181036033	22.082967051027797
39	25.410229158571934	22.6353615048324	30.555403376905126	21.399005959690545
40	26.564661083161745	21.826239326540637	29.793479992347056	21.815619597950562
41	22.043775195516787	23.676937742262556	28.528215186043315	25.751071876177335
42	22.42962533428967	23.397032038709753	28.61460583528801	25.558736791712565
43	23.07009610854374	23.153789683860794	28.72451159783931	25.051602609756156
44	20.509982966292476	22.85921864082645	28.743391115332784	27.88740727754829
45	21.665257726484676	21.38282351612471	27.739826763570285	29.212091993820327
46	23.00140500694918	22.66123655782569	30.121090190994977	24.216268244230157
47	18.803578005699254	23.521181722941396	33.18521902347381	24.490021247885537
48	18.98242772052583	22.238470219668244	32.92461425521568	25.854487804590253
49	22.86848983245271	20.081991047400233	33.983890040295975	23.06562907985109
50	23.209753967859307	18.906403949190498	31.125328811239385	26.758513271710815
51	20.869452350710638	18.549884489380695	29.26898339698147	31.311679762927202
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	255.0
1	726.5
2	1198.0
3	2890.0
4	4582.0
5	3159.5
6	1737.0
7	1805.0
8	1873.0
9	1964.0
10	2055.0
11	1990.0
12	1925.0
13	2046.0
14	2167.0
15	2150.0
16	2133.0
17	2100.0
18	2067.0
19	2204.0
20	2341.0
21	2424.5
22	2508.0
23	3372.5
24	4237.0
25	5068.5
26	7560.5
27	9221.0
28	10966.5
29	12712.0
30	14830.5
31	16949.0
32	19082.5
33	21216.0
34	24696.5
35	28177.0
36	31019.0
37	33861.0
38	37006.5
39	40152.0
40	46111.0
41	52070.0
42	59448.0
43	66826.0
44	76655.5
45	86485.0
46	106815.0
47	127145.0
48	151020.0
49	174895.0
50	165230.0
51	155565.0
52	125142.0
53	94719.0
54	78818.5
55	62918.0
56	54283.0
57	45648.0
58	42602.5
59	39557.0
60	34807.5
61	30058.0
62	25660.5
63	21263.0
64	17224.5
65	13186.0
66	10443.0
67	7700.0
68	6042.5
69	4385.0
70	3536.5
71	2688.0
72	2376.5
73	2065.0
74	1506.0
75	738.0
76	529.0
77	429.5
78	330.0
79	227.5
80	125.0
81	91.0
82	57.0
83	43.0
84	29.0
85	16.0
86	3.0
87	3.0
88	3.0
89	4.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1186471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.650084376678898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.44922577894947	19.7537420813643
2	14.129155483396163	8.661196154746154
3	6.2845298312976166	5.7786410879108265
4	3.4775347879146357	4.26346938689679
5	2.1105102622980265	3.234365880864061
6	1.4034608768688874	2.580971657723949
7	0.956647048315745	2.052491892470488
8	0.7201942148407584	1.7659210761972206
9	0.5717499906370405	1.5771766909851592
>10	5.624182301314373	35.40651269714281
>50	0.18437106886544175	3.7515439802173938
>100	0.07790337884263326	4.461854415569596
>500	0.005544724452261744	1.1479020962993682
>1k	0.003881307116583221	2.2042881249612787
>5k	5.544724452261744E-4	1.3175934491981645
>10k+	5.544724452261744E-4	2.0423293274524625
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT	12030	1.0139312296718588	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	12005	1.0118241406658905	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	8859	0.7466680601548625	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	6647	0.5602328249068035	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	3083	0.2598462162159884	TruSeq Adapter, Index 21 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	3052	0.257233425848588	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2428	0.2046404842596237	No Hit
GCCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	1909	0.16089731649572556	No Hit
TTCCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT	1900	0.16013876445357703	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCC	1758	0.14817049889967812	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTC	1723	0.14522057429132276	No Hit
TGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	1675	0.14117496339986396	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGC	1661	0.1399949935565218	No Hit
GAATCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTC	1518	0.12794244444238417	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCG	1481	0.12482395271355136	No Hit
TTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	1479	0.12465538559307392	TruSeq Adapter, Index 21 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT	1243	0.10476446537673488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.428356023872476E-5	0.0	0.0	1.083718017549523	0.0
2	8.428356023872476E-5	0.0	0.0	3.635992788698586	0.0
3	8.428356023872476E-5	0.0	0.0	5.04546676657078	0.0
4	8.428356023872476E-5	0.0	0.0	6.436819778991648	0.0
5	8.428356023872476E-5	0.0	0.0	8.675475422492417	0.0
6	8.428356023872476E-5	0.0	0.0	9.905341133495888	0.0
7	8.428356023872476E-5	0.0	0.0	11.10806753810249	0.0
8	8.428356023872476E-5	0.0	0.0	12.876926616832607	0.0
9	8.428356023872476E-5	0.0	0.0	13.662449398257522	0.0
10	1.6856712047744952E-4	0.0	0.0	15.386301055820159	0.0
11	1.6856712047744952E-4	0.0	0.0	18.514316826959952	0.0
12	1.6856712047744952E-4	0.0	0.0	21.59100390991436	0.0
13	1.6856712047744952E-4	0.0	0.0	22.56582756763545	3.3713424095489905E-4
14	1.6856712047744952E-4	0.0	0.0	22.934146725878676	3.3713424095489905E-4
15	1.6856712047744952E-4	0.0	0.0	23.499436564399804	3.3713424095489905E-4
16	1.6856712047744952E-4	0.0	0.0	24.63254474824922	3.3713424095489905E-4
17	1.6856712047744952E-4	0.0	0.0	26.226262588803266	3.3713424095489905E-4
18	1.6856712047744952E-4	0.0	0.0	27.90873101828869	3.3713424095489905E-4
19	1.6856712047744952E-4	0.0	0.0	29.150480711285823	3.3713424095489905E-4
20	1.6856712047744952E-4	0.0	0.0	30.560713241200165	3.3713424095489905E-4
21	1.6856712047744952E-4	0.0	0.0	32.37154553292917	3.3713424095489905E-4
22	1.6856712047744952E-4	0.0	0.0	34.25418741798156	3.3713424095489905E-4
23	1.6856712047744952E-4	0.0	0.0	35.880691563468474	3.3713424095489905E-4
24	2.528506807161743E-4	0.0	0.0	37.13710659594714	3.3713424095489905E-4
25	2.528506807161743E-4	0.0	0.0	38.24669966648995	3.3713424095489905E-4
26	2.528506807161743E-4	0.0	0.0	39.200368150591125	3.3713424095489905E-4
27	2.528506807161743E-4	0.0	0.0	40.12588592557256	3.3713424095489905E-4
28	2.528506807161743E-4	0.0	0.0	41.084021438366385	3.3713424095489905E-4
29	2.528506807161743E-4	0.0	0.0	42.056738007081506	3.3713424095489905E-4
30	2.528506807161743E-4	0.0	0.0	43.128150624836174	3.3713424095489905E-4
31	2.528506807161743E-4	0.0	0.0	44.12202236717122	3.3713424095489905E-4
32	2.528506807161743E-4	0.0	0.0	45.09752029337422	3.3713424095489905E-4
33	2.528506807161743E-4	0.0	0.0	46.01637966709679	3.3713424095489905E-4
34	2.528506807161743E-4	0.0	0.0	46.91871946301258	3.3713424095489905E-4
35	2.528506807161743E-4	0.0	0.0	47.80706818792874	3.3713424095489905E-4
36	2.528506807161743E-4	0.0	0.0	48.740508617572615	3.3713424095489905E-4
37	2.528506807161743E-4	0.0	0.0	49.62085040426609	3.3713424095489905E-4
38	2.528506807161743E-4	0.0	0.0	50.53675985338032	3.3713424095489905E-4
39	2.528506807161743E-4	0.0	0.0	51.50087949895109	3.3713424095489905E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGAT	20	7.033378E-4	45.000004	4
GGCGTAA	20	7.033378E-4	45.000004	8
TCTAGCG	45	3.8562575E-10	45.000004	1
AAAGTCG	20	7.033378E-4	45.000004	32
GTGCTCG	30	2.1655615E-6	45.000004	1
CGACTTG	20	7.033378E-4	45.000004	18
CCGTTGT	20	7.033378E-4	45.000004	29
GCGTAAG	20	7.033378E-4	45.000004	44
ACAACGA	25	3.8908827E-5	45.0	27
CGTATAA	35	1.2119744E-7	45.0	28
GACCGAC	25	3.8908827E-5	45.0	14
TTATCCG	25	3.8908827E-5	45.0	1
TTGTTCG	280	0.0	44.196426	1
TCGATTG	95	0.0	42.63158	1
TCTCGCG	95	0.0	42.63158	1
GCGCGAC	120	0.0	41.250004	9
CGAATAG	120	0.0	41.250004	26
AGTGCGA	50	1.0822987E-9	40.5	10
CGTTTTT	1630	0.0	40.030674	1
TACGCGT	45	1.9283107E-8	40.000004	24
>>END_MODULE