##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933963.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	853058
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33946109174288	33.0	31.0	34.0	31.0	34.0
2	32.570152322585336	34.0	31.0	34.0	31.0	34.0
3	32.632553706781955	34.0	31.0	34.0	31.0	34.0
4	36.03413601419833	37.0	35.0	37.0	35.0	37.0
5	36.07781885874114	37.0	35.0	37.0	35.0	37.0
6	35.73277549709398	37.0	35.0	37.0	35.0	37.0
7	36.02923130666379	37.0	35.0	37.0	35.0	37.0
8	36.032058781466205	37.0	35.0	37.0	35.0	37.0
9	37.87653711705418	39.0	38.0	39.0	35.0	39.0
10	37.554389033336534	39.0	37.0	39.0	35.0	39.0
11	37.53610891639256	39.0	37.0	39.0	35.0	39.0
12	37.37473184707253	39.0	37.0	39.0	35.0	39.0
13	37.302868034764344	39.0	37.0	39.0	34.0	39.0
14	38.52503581233633	40.0	38.0	41.0	34.0	41.0
15	38.586057454475544	40.0	38.0	41.0	35.0	41.0
16	38.638062124732436	40.0	38.0	41.0	35.0	41.0
17	38.62157672749098	40.0	38.0	41.0	35.0	41.0
18	38.58334720499661	40.0	38.0	41.0	35.0	41.0
19	38.60903947914444	40.0	38.0	41.0	35.0	41.0
20	38.561226786455315	40.0	38.0	41.0	34.0	41.0
21	38.46890129393312	40.0	38.0	41.0	34.0	41.0
22	38.457630079080204	40.0	38.0	41.0	34.0	41.0
23	38.42063728374858	40.0	38.0	41.0	34.0	41.0
24	38.39478206640111	40.0	37.0	41.0	34.0	41.0
25	38.27627547013216	40.0	37.0	41.0	34.0	41.0
26	38.17375489122662	40.0	37.0	41.0	34.0	41.0
27	38.16289162049943	40.0	37.0	41.0	34.0	41.0
28	38.11547163264397	40.0	37.0	41.0	34.0	41.0
29	38.11576586820591	40.0	37.0	41.0	34.0	41.0
30	38.095691031559404	40.0	37.0	41.0	34.0	41.0
31	38.021514363618884	40.0	37.0	41.0	34.0	41.0
32	37.9301114343925	40.0	37.0	41.0	34.0	41.0
33	37.880165240815984	40.0	37.0	41.0	34.0	41.0
34	37.83972250421425	40.0	37.0	41.0	33.0	41.0
35	37.78286001655221	40.0	37.0	41.0	33.0	41.0
36	37.67480054111209	40.0	36.0	41.0	33.0	41.0
37	37.621858068267336	40.0	36.0	41.0	33.0	41.0
38	37.58931866297485	40.0	36.0	41.0	33.0	41.0
39	37.58577728595242	40.0	36.0	41.0	33.0	41.0
40	37.48889876186613	40.0	36.0	41.0	33.0	41.0
41	37.36944850174314	40.0	36.0	41.0	33.0	41.0
42	37.3181589059595	39.0	35.0	41.0	33.0	41.0
43	37.19816120357584	39.0	35.0	41.0	32.0	41.0
44	37.073905877443266	39.0	35.0	41.0	32.0	41.0
45	37.11134295675089	39.0	35.0	41.0	32.0	41.0
46	37.03969835579761	39.0	35.0	41.0	32.0	41.0
47	36.967664566770374	39.0	35.0	41.0	32.0	41.0
48	36.928056474471845	39.0	35.0	41.0	32.0	41.0
49	36.93338084866445	39.0	35.0	41.0	32.0	41.0
50	36.863283621981154	39.0	35.0	41.0	32.0	41.0
51	35.90458327569755	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	11.0
10	19.0
11	15.0
12	2.0
13	15.0
14	17.0
15	18.0
16	29.0
17	38.0
18	93.0
19	174.0
20	302.0
21	510.0
22	837.0
23	1288.0
24	1869.0
25	2805.0
26	3988.0
27	5031.0
28	6148.0
29	7703.0
30	9767.0
31	12714.0
32	16856.0
33	24241.0
34	45055.0
35	63012.0
36	60512.0
37	96686.0
38	190131.0
39	302976.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.166117661401685	4.544474115476322	54.87844906208019	16.410959161041806
2	19.726208534472452	4.7703673138286025	53.283832986737124	22.21959116496182
3	20.811011677986727	4.542598510300589	53.093107385429825	21.553282426282856
4	19.799239911002534	4.785137704587496	51.04459485755951	24.37102752685046
5	18.100996649700253	5.6787463455005405	50.23878798393544	25.981469020863763
6	21.326451425342707	6.253267655892096	52.58306000295407	19.83722091581112
7	81.70476098928795	2.3074632674448865	10.338687404607894	5.649088338659271
8	82.78944690747873	2.994403663056908	7.986795739562844	6.229353689901507
9	76.73112496453935	5.637131355663976	11.22561420208239	6.406129477714294
10	34.37773281535371	33.90050852345327	17.10329192153406	14.618466739658967
11	29.65800684126988	25.088212055921165	25.96329909572386	19.2904820070851
12	27.942883133386005	21.663825906327588	30.436500214522344	19.956790745764064
13	24.078550344759677	23.83624560111974	30.745154491253817	21.34004956286677
14	18.936930431459526	27.63493220859543	30.06372368584551	23.36441367409953
15	17.31629033430318	25.967636432692736	35.14673093740402	21.569342295600062
16	18.596508092064077	24.90534055128725	33.17136701138727	23.3267843452614
17	18.86776749060439	24.44359000208661	30.336858689561552	26.351783817747442
18	19.71518935406502	24.69878953130971	31.42740587392651	24.158615240698754
19	22.60162849419383	25.2737797429952	29.657186264005496	22.467405498805473
20	26.426456348806294	25.294059723957808	28.987595216269003	19.291888710966898
21	24.430343540533002	26.503238935687847	28.570273064668523	20.496144459110635
22	21.39115980390548	25.43308895760898	27.739731647789483	25.436019590696063
23	21.842360073992626	26.15543140091295	27.61711395942597	24.385094565668457
24	22.369991254990868	22.9608068853466	28.879865143987864	25.789336715674665
25	20.7529851428625	26.19657748945558	26.807673100773922	26.24276426690799
26	20.529436450979887	28.816915145277346	27.45065400007971	23.20299440366306
27	22.048207742029263	26.987027845703338	28.061749611398053	22.903014800869343
28	18.59615641609363	26.204548811452444	30.204276848701966	24.99501792375196
29	19.745902388817644	24.518379758468946	31.055918823807993	24.679799028905418
30	21.421052261393715	23.391258273177204	31.406774216993448	23.780915248435626
31	20.448433752452942	26.46021724196948	28.611653603858123	24.47969540171946
32	21.808833631476407	26.648832787454076	27.562955859976697	23.97937772109282
33	23.488555291668327	24.671358805614624	27.79857876017809	24.041507142538958
34	19.802991121354	24.113952392451626	30.20521465128983	25.87784183490454
35	19.93615908883101	24.183349783953727	28.43054047907645	27.449950648138817
36	21.77460383701929	26.22072590609314	27.860590956300747	24.144079300586828
37	21.439573862504073	25.28772955648971	29.547697811872116	23.724998769134103
38	19.371015804318112	24.830433569581437	29.87932825200631	25.91922237409414
39	21.38084397543895	21.958178693594103	33.132213753343855	23.528763577623092
40	21.84259452463959	21.84259452463959	33.48037296408919	22.834437986631624
41	19.220264038318614	24.045375578213907	29.512647440150612	27.221712943316867
42	22.980969640985723	23.55080193843795	26.556224781902287	26.912003638674044
43	23.697568043439016	23.009103718621713	28.138766648926566	25.1545615890127
44	21.566646113159948	22.51546788143362	29.479472673604846	26.43841333180159
45	20.58382900107613	22.012571243690346	29.176210761753595	28.22738899347993
46	20.864349200171617	22.819198694578798	30.941624133411793	25.37482797183779
47	18.641639841605144	23.17228136891044	33.03585453744059	25.150224252043824
48	19.23585500634189	21.33688447913272	32.37341423443658	27.053846280088813
49	21.206881595389763	19.763251736693167	33.94540582234737	25.084460845569705
50	20.372354517512292	19.969685531347224	31.79045270075423	27.867507250386257
51	18.492998131428344	19.886103875703647	29.883196687681256	31.73770130518675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	122.0
1	508.0
2	894.0
3	1974.5
4	3055.0
5	2093.5
6	1132.0
7	1104.0
8	1076.0
9	1191.5
10	1307.0
11	1338.0
12	1369.0
13	1281.5
14	1194.0
15	1209.0
16	1224.0
17	1286.0
18	1348.0
19	1263.5
20	1179.0
21	1606.5
22	2034.0
23	2575.0
24	3116.0
25	3914.0
26	5794.0
27	6876.0
28	8089.0
29	9302.0
30	10513.0
31	11724.0
32	13727.0
33	15730.0
34	18336.5
35	20943.0
36	22955.0
37	24967.0
38	28155.5
39	31344.0
40	35356.5
41	39369.0
42	44968.0
43	50567.0
44	55755.5
45	60944.0
46	71495.0
47	82046.0
48	96426.5
49	110807.0
50	106735.0
51	102663.0
52	84567.5
53	66472.0
54	57415.5
55	48359.0
56	42697.0
57	37035.0
58	34739.5
59	32444.0
60	30040.5
61	27637.0
62	22986.5
63	18336.0
64	15266.5
65	12197.0
66	10095.5
67	7994.0
68	6223.5
69	4453.0
70	3812.0
71	3171.0
72	2421.0
73	1671.0
74	1418.5
75	826.0
76	486.0
77	417.5
78	349.0
79	252.5
80	156.0
81	88.5
82	21.0
83	38.5
84	56.0
85	31.5
86	7.0
87	5.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	853058.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.109875367772798
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.20815809448169	18.32993196997905
2	13.732267280448603	7.720246435407089
3	5.965937031406841	5.031052392144803
4	3.2729680019635805	3.680108904716183
5	1.9546527474687145	2.747252255931012
6	1.3063255337487856	2.203238876605058
7	0.9045915133934816	1.779956829016502
8	0.6744758832107735	1.5167546412498658
9	0.536677582524539	1.357734596169815
>10	5.917194354448456	39.666045717826215
>50	0.43193662206169475	7.550232527309079
>100	0.08638732330132087	4.223173992712717
>500	0.004635417347875753	0.7884395292128308
>1k	0.002949811039557297	1.9458678104536016
>5k	8.428031541592279E-4	1.4599635212662472
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT	6258	0.7335960743583672	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC	6067	0.7112060375730607	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC	4770	0.5591647930152464	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC	4746	0.5563513852516476	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTG	1704	0.19975195121550937	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1629	0.19096005195426338	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTG	1235	0.14477327450185098	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTG	1224	0.14348379594353491	No Hit
TGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTG	1119	0.13117513697779049	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCT	925	0.10843342422203414	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5167597044984047E-4	0.0	0.0	0.7629024052291872	0.0
2	3.5167597044984047E-4	0.0	0.0	2.716462421078051	0.0
3	3.5167597044984047E-4	0.0	0.0	3.740425621704503	0.0
4	3.5167597044984047E-4	0.0	0.0	4.772360144327818	0.0
5	3.5167597044984047E-4	0.0	0.0	6.407887857566543	0.0
6	3.5167597044984047E-4	0.0	0.0	7.398324615676777	0.0
7	3.5167597044984047E-4	0.0	0.0	8.305765844760849	0.0
8	3.5167597044984047E-4	0.0	0.0	9.754436392367225	0.0
9	3.5167597044984047E-4	0.0	0.0	10.438680605539131	0.0
10	3.5167597044984047E-4	0.0	0.0	11.865078341683684	0.0
11	3.5167597044984047E-4	0.0	0.0	14.309695237604009	0.0
12	4.689012939331206E-4	0.0	0.0	16.707890905424954	0.0
13	4.689012939331206E-4	0.0	0.0	17.44687934466355	0.0
14	4.689012939331206E-4	0.0	0.0	17.69844488885867	0.0
15	4.689012939331206E-4	0.0	0.0	18.102989480199472	0.0
16	4.689012939331206E-4	0.0	0.0	19.05497633220719	0.0
17	4.689012939331206E-4	0.0	0.0	20.355239620283733	0.0
18	4.689012939331206E-4	0.0	0.0	21.868501321129397	0.0
19	4.689012939331206E-4	0.0	0.0	22.857296924710862	0.0
20	4.689012939331206E-4	0.0	0.0	23.949954164898518	0.0
21	4.689012939331206E-4	0.0	0.0	25.398038585887477	0.0
22	4.689012939331206E-4	0.0	0.0	26.87273315530714	0.0
23	4.689012939331206E-4	0.0	0.0	28.23395361159499	0.0
24	4.689012939331206E-4	0.0	0.0	29.24173971758075	0.0
25	4.689012939331206E-4	0.0	0.0	30.121398544999284	0.0
26	4.689012939331206E-4	0.0	0.0	30.920406349861324	0.0
27	4.689012939331206E-4	0.0	0.0	31.651306241779576	0.0
28	4.689012939331206E-4	0.0	0.0	32.438005387675865	0.0
29	4.689012939331206E-4	0.0	0.0	33.30570723209911	0.0
30	4.689012939331206E-4	0.0	0.0	34.303529185588786	0.0
31	5.861266174164007E-4	0.0	0.0	35.21601110358264	0.0
32	5.861266174164007E-4	0.0	0.0	36.10434460493894	0.0
33	5.861266174164007E-4	0.0	0.0	36.90440743771232	0.0
34	5.861266174164007E-4	0.0	0.0	37.664965336471845	0.0
35	5.861266174164007E-4	0.0	0.0	38.474523420447376	0.0
36	7.033519408996809E-4	0.0	0.0	39.293459530301575	0.0
37	7.033519408996809E-4	0.0	0.0	40.11755355438903	0.0
38	7.033519408996809E-4	0.0	0.0	40.893585195848345	0.0
39	7.033519408996809E-4	0.0	0.0	41.6630522191926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	7.032262E-4	45.000004	24
TCGTTAG	40	6.8121153E-9	45.000004	1
ACACGTC	20	7.032262E-4	45.000004	37
ACGTTGA	20	7.032262E-4	45.000004	12
TTACTCG	40	6.8121153E-9	45.000004	1
TGTTCGA	20	7.032262E-4	45.000004	31
TACTCCG	20	7.032262E-4	45.000004	20
ACGTCGG	20	7.032262E-4	45.000004	39
CCGACAC	20	7.032262E-4	45.000004	11
TTACGTA	20	7.032262E-4	45.000004	19
AACCTCG	20	7.032262E-4	45.000004	43
CGTCGGC	20	7.032262E-4	45.000004	40
TCCCCGT	20	7.032262E-4	45.000004	19
CACGTCG	20	7.032262E-4	45.000004	38
TGTACCG	20	7.032262E-4	45.000004	1
TGTCGCG	20	7.032262E-4	45.000004	1
CCGTTTA	20	7.032262E-4	45.000004	23
TACCGTT	20	7.032262E-4	45.000004	21
TTGATCG	25	3.8899576E-5	45.0	13
CGGCGAA	50	2.1827873E-11	45.0	13
>>END_MODULE