##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933961.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	754154
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.452635668576974	33.0	31.0	34.0	31.0	34.0
2	32.68578698780355	34.0	31.0	34.0	31.0	34.0
3	32.73462979709714	34.0	31.0	34.0	31.0	34.0
4	36.09626018028148	37.0	35.0	37.0	35.0	37.0
5	36.13946620981921	37.0	35.0	37.0	35.0	37.0
6	35.807747489239595	37.0	35.0	37.0	35.0	37.0
7	36.11227547689199	37.0	35.0	37.0	35.0	37.0
8	36.11052119328413	37.0	37.0	37.0	35.0	37.0
9	37.974302330823676	39.0	39.0	39.0	35.0	39.0
10	37.65743203642757	39.0	37.0	39.0	35.0	39.0
11	37.60718235267598	39.0	37.0	39.0	35.0	39.0
12	37.439268372242275	39.0	37.0	39.0	35.0	39.0
13	37.37015914521437	39.0	37.0	39.0	35.0	39.0
14	38.55092328622536	40.0	38.0	41.0	35.0	41.0
15	38.61478159633178	40.0	38.0	41.0	35.0	41.0
16	38.698801571031915	40.0	38.0	41.0	35.0	41.0
17	38.67876587540476	40.0	38.0	41.0	35.0	41.0
18	38.643391137619105	40.0	38.0	41.0	35.0	41.0
19	38.65991296207406	40.0	38.0	41.0	35.0	41.0
20	38.61880729930492	40.0	38.0	41.0	35.0	41.0
21	38.54948591401756	40.0	38.0	41.0	34.0	41.0
22	38.540139016699506	40.0	38.0	41.0	34.0	41.0
23	38.50197970175853	40.0	38.0	41.0	34.0	41.0
24	38.489048655844826	40.0	38.0	41.0	34.0	41.0
25	38.380155777202006	40.0	37.0	41.0	34.0	41.0
26	38.331197606854836	40.0	37.0	41.0	34.0	41.0
27	38.33908459015002	40.0	37.0	41.0	34.0	41.0
28	38.26361194132763	40.0	37.0	41.0	34.0	41.0
29	38.19453453803865	40.0	37.0	41.0	34.0	41.0
30	38.163831259928344	40.0	37.0	41.0	34.0	41.0
31	38.05286453429936	40.0	37.0	41.0	34.0	41.0
32	37.885502165340235	40.0	36.0	41.0	34.0	41.0
33	37.85158071163184	40.0	37.0	41.0	34.0	41.0
34	37.840651909291736	40.0	37.0	41.0	33.0	41.0
35	37.75545445625164	40.0	36.0	41.0	33.0	41.0
36	37.716211808198324	40.0	36.0	41.0	33.0	41.0
37	37.66457116185819	40.0	36.0	41.0	33.0	41.0
38	37.56826854992482	40.0	36.0	41.0	33.0	41.0
39	37.54505180639498	40.0	36.0	41.0	33.0	41.0
40	37.469649434996036	40.0	36.0	41.0	33.0	41.0
41	37.32028471638419	39.0	35.0	41.0	33.0	41.0
42	37.27774565937461	39.0	35.0	41.0	33.0	41.0
43	37.231402339575205	39.0	35.0	41.0	33.0	41.0
44	37.15877658939686	39.0	35.0	41.0	32.0	41.0
45	37.204850733404584	39.0	35.0	41.0	33.0	41.0
46	37.105312708014544	39.0	35.0	41.0	32.0	41.0
47	37.01723255462412	39.0	35.0	41.0	32.0	41.0
48	36.918022048547115	39.0	35.0	41.0	32.0	41.0
49	36.9114053628304	39.0	35.0	41.0	32.0	41.0
50	36.82418312440165	39.0	35.0	41.0	32.0	41.0
51	35.74602667359717	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	13.0
10	16.0
11	21.0
12	7.0
13	9.0
14	18.0
15	20.0
16	18.0
17	49.0
18	73.0
19	167.0
20	239.0
21	468.0
22	747.0
23	1054.0
24	1531.0
25	2362.0
26	3316.0
27	4053.0
28	5050.0
29	6122.0
30	8069.0
31	10327.0
32	14245.0
33	20913.0
34	40944.0
35	53912.0
36	53623.0
37	86737.0
38	174199.0
39	265645.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.118161542602703	4.790666097375337	56.715074109532004	16.376098250489953
2	19.976158715593897	4.881363753291768	53.3987753164473	21.74370221466703
3	20.507349957700946	4.669603290574604	52.04043206029537	22.78261469142907
4	19.59745622246915	5.022846792564914	51.00244777591845	24.377249209047488
5	18.76526544976225	5.716339103153998	50.26387183519546	25.254523611888285
6	22.16152138687854	5.814462298151306	52.706211198243324	19.31780511672682
7	82.33729450483588	2.315840000848633	9.895724215478536	5.451141278836948
8	82.88943637506398	3.1339752888667296	8.041858824590204	5.934729511479088
9	77.21791570421956	5.397703917237063	10.924691773828688	6.459688604714688
10	35.21601688779745	32.87962405556425	17.45677938458193	14.44757967205637
11	28.35256459556006	26.12105750284425	26.297414056015082	19.22896384558061
12	28.202197429172294	21.170874914142203	30.651962331301036	19.97496532538447
13	25.166875730951503	23.069028341691485	31.28618292815526	20.477912999201756
14	19.262113573620244	26.371006452263067	30.54468981136479	23.822190162751905
15	16.987777032277226	26.268507493164527	34.90997859853558	21.83373687602267
16	19.69743580223668	25.131471821405178	32.5808787064711	22.590213669887053
17	19.449608435412397	24.967181769240764	30.312243918350894	25.270965876995945
18	20.647507007852507	24.42856498805284	31.734897646899707	23.18903035719495
19	22.10967521222456	26.56645724878473	29.481909530414214	21.841958008576498
20	24.87171055248663	26.19518029474086	29.88660141032203	19.04650774245048
21	24.356828976575077	26.571496007446754	29.34427185959366	19.727403156384504
22	21.604075560164105	25.001922684226297	28.958806821948833	24.435194933660764
23	21.022231533612498	25.989386783070834	29.50392094983253	23.48446073348414
24	22.702524948485323	23.303728416212074	29.240446911373542	24.753299723929064
25	20.1216197222318	25.599811179149086	28.37285222912031	25.9057168694988
26	19.81226646016596	26.321281860203623	29.53269491377093	24.333756765859494
27	20.2670807288697	26.315447508068644	29.37781938436977	24.039652378691887
28	19.308523192875725	24.807532679001902	31.585458672897047	24.298485455225325
29	20.183145617473354	23.47027264988318	32.32429981144435	24.022281921199117
30	23.66784502899938	23.31035836182	29.760897641595747	23.26089896758487
31	23.689060854944746	26.011530801401307	27.70057574447659	22.598832599177356
32	22.639540465210022	26.84929072841886	28.56021449200031	21.950954314370804
33	24.048801703630822	26.170649495991533	26.943701153875733	22.83684764650191
34	22.07639288527277	24.24239611538227	30.20921456360372	23.47199643574124
35	19.77977972668712	23.573832400278988	30.745179366548474	25.901208506485414
36	21.38077899208915	25.322944650562086	29.177064631361766	24.119211725987
37	22.156615227128675	25.624739774634893	29.682664283422216	22.535980714814215
38	22.13102363708208	25.53112494265097	29.99705630415008	22.34079511611687
39	23.755492909936166	23.125648077183175	30.33863110187044	22.78022791101022
40	25.676055553640236	22.141366352230445	30.63445927489611	21.548118819233206
41	20.57245602357078	24.12995223787184	30.44046706640819	24.85712467214919
42	20.663816674048004	24.755951702172236	29.617823415376698	24.96240820840306
43	22.269987297024215	23.728840528592304	29.536142485487048	24.465029688896433
44	21.78613386655776	22.761531464395865	29.339896095492435	26.11243857355394
45	22.926086714384596	20.854361310819804	28.29395587638599	27.92559609840961
46	22.380044394115792	22.032900442084774	30.735234448136588	24.851820715662846
47	20.17015092408182	23.093426541528654	32.99830008194613	23.7381224524434
48	20.15317826332553	21.909716052689504	32.803512279985256	25.133593403999715
49	22.11047080569751	20.760348682099412	33.477910347223514	23.651270164979564
50	22.118426740427022	20.61568326893446	31.086621565356676	26.179268425281837
51	20.645650623082286	20.362684544536	29.254369797150183	29.737295035231533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	153.0
1	476.5
2	800.0
3	1631.5
4	2463.0
5	1687.0
6	911.0
7	975.5
8	1040.0
9	1088.5
10	1137.0
11	1175.5
12	1214.0
13	1103.5
14	993.0
15	1094.0
16	1195.0
17	1305.5
18	1416.0
19	1706.0
20	1996.0
21	1990.5
22	1985.0
23	2368.5
24	2752.0
25	3560.5
26	5434.0
27	6499.0
28	8039.5
29	9580.0
30	10303.5
31	11027.0
32	13209.0
33	15391.0
34	18299.0
35	21207.0
36	22512.0
37	23817.0
38	26290.0
39	28763.0
40	32231.5
41	35700.0
42	39151.0
43	42602.0
44	48471.0
45	54340.0
46	62282.5
47	70225.0
48	81707.0
49	93189.0
50	90727.5
51	88266.0
52	73451.0
53	58636.0
54	50413.0
55	42190.0
56	37695.0
57	33200.0
58	30348.5
59	27497.0
60	26513.0
61	25529.0
62	21334.0
63	17139.0
64	14071.5
65	11004.0
66	8675.0
67	6346.0
68	5233.0
69	4120.0
70	3151.0
71	2182.0
72	1829.0
73	1476.0
74	1189.0
75	693.0
76	484.0
77	320.0
78	156.0
79	154.0
80	152.0
81	119.5
82	87.0
83	52.0
84	17.0
85	9.5
86	2.0
87	3.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	754154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.915759327815994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.358136958706	19.851540546989003
2	13.654002740212764	8.169397196750904
3	5.690263037477599	5.1068561862344115
4	2.994519590958849	3.583333095422197
5	1.768945910731599	2.64596800646854
6	1.1302354317888907	2.0287110696699986
7	0.7742492252367911	1.6213577437352287
8	0.5816321745441617	1.3919974520781926
9	0.4775358141847895	1.285726183880834
>10	6.300242015937313	43.430287416430886
>50	0.19417698931218477	3.6593721153861316
>100	0.06890151223527329	3.540114592592832
>500	0.003579299336897313	0.7250482342650146
>1k	0.003131886919785149	2.1644369570425606
>5k	4.4741241711216415E-4	0.7958532030533094
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	5946	0.7884331316945875	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	4508	0.5977558960106292	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	3566	0.4728477207572989	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	3016	0.39991831907011033	TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	1466	0.19439000522439714	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1312	0.17396977275198436	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	1271	0.16853321735348484	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	1032	0.13684207734759743	TruSeq Adapter, Index 21 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	910	0.12066501006425745	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT	842	0.11164828403747776	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3259891215852465E-4	0.0	0.0	0.6209607056383709	0.0
2	1.3259891215852465E-4	0.0	0.0	2.3938081611978457	0.0
3	1.3259891215852465E-4	0.0	0.0	3.4145545869941683	0.0
4	1.3259891215852465E-4	0.0	0.0	4.413156994460017	0.0
5	1.3259891215852465E-4	0.0	0.0	6.0142888587742025	0.0
6	1.3259891215852465E-4	0.0	0.0	6.933597116769254	0.0
7	1.3259891215852465E-4	0.0	0.0	7.785279929563458	0.0
8	1.3259891215852465E-4	0.0	0.0	9.038339649461516	0.0
9	1.3259891215852465E-4	0.0	0.0	9.619255483628013	0.0
10	1.3259891215852465E-4	0.0	0.0	10.815430270210063	0.0
11	1.3259891215852465E-4	0.0	0.0	13.150762311146	0.0
12	1.3259891215852465E-4	0.0	0.0	15.290245758823795	0.0
13	1.3259891215852465E-4	0.0	0.0	15.979892700960281	0.0
14	1.3259891215852465E-4	0.0	0.0	16.223344303683334	0.0
15	1.3259891215852465E-4	0.0	0.0	16.61384809999019	0.0
16	1.3259891215852465E-4	0.0	0.0	17.546946644849726	0.0
17	1.3259891215852465E-4	0.0	0.0	18.78767466591704	0.0
18	1.3259891215852465E-4	0.0	0.0	20.134747014535492	0.0
19	1.3259891215852465E-4	0.0	0.0	21.056707250773716	0.0
20	1.3259891215852465E-4	0.0	0.0	22.14653770980463	0.0
21	1.3259891215852465E-4	0.0	0.0	23.591865852332546	0.0
22	1.3259891215852465E-4	0.0	0.0	25.089835762987402	0.0
23	1.3259891215852465E-4	0.0	0.0	26.42457641277511	0.0
24	2.651978243170493E-4	0.0	0.0	27.405808362748193	0.0
25	2.651978243170493E-4	0.0	0.0	28.33347035220923	0.0
26	2.651978243170493E-4	0.0	0.0	29.11805811545122	0.0
27	2.651978243170493E-4	0.0	0.0	29.865650782200984	0.0
28	2.651978243170493E-4	0.0	0.0	30.673841151807192	0.0
29	2.651978243170493E-4	0.0	0.0	31.503645144095238	0.0
30	2.651978243170493E-4	0.0	0.0	32.48792686904797	0.0
31	2.651978243170493E-4	0.0	0.0	33.36307968929423	0.0
32	3.9779673647557395E-4	0.0	0.0	34.196331253298396	0.0
33	3.9779673647557395E-4	0.0	0.0	34.96898511444612	0.0
34	3.9779673647557395E-4	0.0	0.0	35.722809930067335	0.0
35	3.9779673647557395E-4	0.0	0.0	36.56680200595634	0.0
36	3.9779673647557395E-4	0.0	0.0	37.41742402745328	0.0
37	3.9779673647557395E-4	0.0	0.0	38.26128350443013	0.0
38	3.9779673647557395E-4	0.0	0.0	39.0596615545366	0.0
39	3.9779673647557395E-4	0.0	0.0	39.93799674867467	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGGA	20	7.031741E-4	45.000004	8
GGCCGTA	20	7.031741E-4	45.000004	8
CGTTCAT	20	7.031741E-4	45.000004	17
CCCCGTA	20	7.031741E-4	45.000004	16
TAGATGG	20	7.031741E-4	45.000004	2
CCGCGAA	20	7.031741E-4	45.000004	30
CCTATCG	20	7.031741E-4	45.000004	33
TCGAAAG	20	7.031741E-4	45.000004	28
GTACGAA	20	7.031741E-4	45.000004	28
GCCGATC	25	3.8895247E-5	45.0	9
TCATGCG	35	1.2111923E-7	45.0	1
TTGCGGA	25	3.8895247E-5	45.0	3
AACGACG	25	3.8895247E-5	45.0	11
TGTTGCG	80	0.0	42.187504	1
TCTAGCG	60	3.6379788E-12	41.249996	1
TCGTTTG	280	0.0	40.98214	1
CGTTTTT	855	0.0	40.000004	1
TGTTTCG	210	0.0	39.642857	1
TTCGTTG	245	0.0	39.489796	1
CGCATGG	40	3.4561162E-7	39.375004	2
>>END_MODULE