SRR2933960.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933960.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	523046
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	4705	0.899538472715593	RNA PCR Primer, Index 36 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	2968	0.5674453107374877	RNA PCR Primer, Index 36 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2468	0.47185142415772224	RNA PCR Primer, Index 15 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2307	0.44107019267903785	RNA PCR Primer, Index 15 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG	881	0.16843642815354673	RNA PCR Primer, Index 36 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG	806	0.1540973451665819	RNA PCR Primer, Index 36 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	759	0.14511151982808396	No Hit
GCCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG	711	0.13593450671642646	RNA PCR Primer, Index 15 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	125	0.0	45.000004	1
CACGGCG	25	3.8878796E-5	45.0	21
CGAAACT	20	7.029758E-4	45.0	8
CGAAACG	25	3.8878796E-5	45.0	14
TCTTACG	20	7.029758E-4	45.0	1
GCACGGG	55	1.8189894E-12	45.0	3
TAACGGA	20	7.029758E-4	45.0	22
TCTAGCG	20	7.029758E-4	45.0	1
CACTCGT	20	7.029758E-4	45.0	27
ATTTTCG	25	3.8878796E-5	45.0	14
GTTCGTA	20	7.029758E-4	45.0	30
GTTAACG	20	7.029758E-4	45.0	20
CTCGGTA	20	7.029758E-4	45.0	11
CGGATAT	25	3.8878796E-5	45.0	14
CGTAGAC	20	7.029758E-4	45.0	31
CGCTAGG	20	7.029758E-4	45.0	2
ACGGATA	25	3.8878796E-5	45.0	13
GTCGTGG	25	3.8878796E-5	45.0	2
CGCAGAC	20	7.029758E-4	45.0	37
CGAGAGT	20	7.029758E-4	45.0	14