##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933960.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	523046
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.421077687239745	33.0	31.0	34.0	31.0	34.0
2	32.64299698305694	34.0	31.0	34.0	31.0	34.0
3	32.71664251327799	34.0	31.0	34.0	31.0	34.0
4	36.07273738829854	37.0	35.0	37.0	35.0	37.0
5	36.112647835945594	37.0	35.0	37.0	35.0	37.0
6	35.75450725175224	37.0	35.0	37.0	35.0	37.0
7	36.07112949912627	37.0	35.0	37.0	35.0	37.0
8	36.0653460689882	37.0	36.0	37.0	35.0	37.0
9	37.90762380364251	39.0	38.0	39.0	35.0	39.0
10	37.605782283011436	39.0	37.0	39.0	35.0	39.0
11	37.57051578637443	39.0	37.0	39.0	35.0	39.0
12	37.34668652470337	39.0	37.0	39.0	35.0	39.0
13	37.24023317260814	39.0	37.0	39.0	34.0	39.0
14	38.46517132336353	40.0	38.0	41.0	34.0	41.0
15	38.54119331760495	40.0	38.0	41.0	35.0	41.0
16	38.59800858815477	40.0	38.0	41.0	35.0	41.0
17	38.570699326636664	40.0	38.0	41.0	35.0	41.0
18	38.51739426360206	40.0	38.0	41.0	34.0	41.0
19	38.5414017122777	40.0	38.0	41.0	34.0	41.0
20	38.50802223896177	40.0	38.0	41.0	34.0	41.0
21	38.428579895458526	40.0	37.0	41.0	34.0	41.0
22	38.419901882434814	40.0	37.0	41.0	34.0	41.0
23	38.366904631714995	40.0	37.0	41.0	34.0	41.0
24	38.33915946207408	40.0	37.0	41.0	34.0	41.0
25	38.255969838216906	40.0	37.0	41.0	34.0	41.0
26	38.18411382555263	40.0	37.0	41.0	34.0	41.0
27	38.159400893994025	40.0	37.0	41.0	34.0	41.0
28	38.06324300348344	40.0	37.0	41.0	34.0	41.0
29	38.06841845650287	40.0	37.0	41.0	34.0	41.0
30	37.9547190878049	40.0	36.0	41.0	34.0	41.0
31	37.8522978858456	40.0	36.0	41.0	34.0	41.0
32	37.808079977669266	40.0	36.0	41.0	33.0	41.0
33	37.76420429560689	40.0	36.0	41.0	33.0	41.0
34	37.71054553519193	40.0	36.0	41.0	33.0	41.0
35	37.705454204792694	40.0	36.0	41.0	33.0	41.0
36	37.59029033775232	40.0	36.0	41.0	33.0	41.0
37	37.48584445727527	40.0	36.0	41.0	33.0	41.0
38	37.45232541688494	40.0	35.0	41.0	33.0	41.0
39	37.400008794637564	40.0	35.0	41.0	33.0	41.0
40	37.25162414013299	39.0	35.0	41.0	32.0	41.0
41	37.161555580197536	39.0	35.0	41.0	32.0	41.0
42	37.18491107856671	39.0	35.0	41.0	32.0	41.0
43	37.06437292322281	39.0	35.0	41.0	32.0	41.0
44	36.89805294371814	39.0	35.0	41.0	31.0	41.0
45	36.89364032991362	39.0	35.0	41.0	31.0	41.0
46	36.77407914409058	39.0	35.0	41.0	31.0	41.0
47	36.686394695686424	39.0	35.0	41.0	31.0	41.0
48	36.63454648348329	39.0	35.0	41.0	31.0	41.0
49	36.574029817645105	39.0	35.0	40.0	31.0	41.0
50	36.40172757271827	38.0	35.0	40.0	31.0	41.0
51	35.390003938468126	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	11.0
11	7.0
12	9.0
13	9.0
14	13.0
15	15.0
16	20.0
17	47.0
18	60.0
19	114.0
20	224.0
21	367.0
22	565.0
23	855.0
24	1288.0
25	1820.0
26	2727.0
27	3408.0
28	4073.0
29	4923.0
30	6202.0
31	7855.0
32	10627.0
33	15321.0
34	30062.0
35	42115.0
36	36400.0
37	59084.0
38	114594.0
39	180092.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.542009689396345	4.843933420769875	52.66821656221441	17.945840327619365
2	18.541007865464987	4.5946245645698465	53.60350714851084	23.26086042145433
3	21.366189589443376	4.798048355211588	51.30198873521641	22.53377332012863
4	20.43912007739281	5.319799788163947	50.88539057750179	23.355689556941456
5	19.038478451226087	6.148025221491035	49.98680804365199	24.826688283630887
6	22.210665983489026	6.426394619211312	52.17648160964809	19.186457787651566
7	82.52333446771412	2.4179517671485873	9.691499409229781	5.367214355907511
8	82.69540346355771	3.601786458552403	7.784974935282938	5.91783514260696
9	76.87029439093311	6.137318706194102	10.774769331951683	6.217617570921105
10	36.30044011425381	34.0314236223965	16.458590640211376	13.209545623138307
11	31.625899060503283	25.69276889604356	24.268611173778215	18.412720869674942
12	30.55562990635623	21.35127694313693	28.27667165029462	19.81642150021222
13	24.773920458238855	24.23744756675321	28.97565414896587	22.012977826042068
14	18.75666767358894	26.800510853729882	29.586116708664246	24.856704764016932
15	18.73123969975872	26.072850189084708	34.53042371034288	20.665486400813695
16	21.21476887310103	25.113852318916503	32.72064024961476	20.950738558367714
17	20.745020514448058	24.639133078161386	29.187107826080304	25.42873858131025
18	21.157030165606848	25.75815511446412	30.557350596314663	22.527464123614365
19	23.369646264382098	26.17016476562291	28.21052068078142	22.249668289213567
20	26.008993472849422	25.78262714942854	28.86075029729699	19.34762908042505
21	25.657016782462726	25.163752327711137	28.968962576905284	20.21026831292085
22	22.70144499718954	25.45550486955258	27.67768035698581	24.165369776272065
23	23.101792194185595	25.217476091968965	27.960638261261916	23.72009345258352
24	24.169767095054738	22.33952654259855	28.916194751513252	24.574511610833465
25	21.9523713019505	25.469270389220068	27.20735843501336	25.370999873816068
26	22.767404778929578	26.758067168088466	27.3545730203462	23.11995503263575
27	23.207136657196497	27.062246915185277	27.97975703857787	21.750859389040354
28	19.349158582610325	28.099822960122054	28.90147329297997	23.64954516428765
29	22.033817293316456	25.56907040680934	28.506479353632376	23.890632946241823
30	22.784802866287095	26.869529639840472	27.423018243137314	22.922649250735116
31	22.257506987913107	29.802158892334518	25.463152380478967	22.477181739273412
32	23.70862218619395	27.944004924997035	26.117205752457718	22.230167136351294
33	22.663781005877112	27.775759684616652	25.53064166440428	24.02981764510196
34	20.901794488438874	26.837601281722833	28.604367493490056	23.656236736348237
35	21.463886541527895	24.664178676445285	27.408870347923507	26.463064434103313
36	23.123778788098946	28.49252264619173	25.716858555461663	22.666840010247665
37	21.436355502192924	29.338146166876335	27.524347762911866	21.701150568018875
38	19.69807626862647	28.77720124042627	26.581600853462216	24.94312163748504
39	20.968327833498392	26.629015421205782	27.750905274105907	24.651751471189915
40	23.02264045609755	25.439827472153503	28.945064105260343	22.59246796648861
41	18.7889784072529	26.261552521193167	27.978992287485234	26.970476784068705
42	21.582231773113648	24.830129663547755	27.069512050565343	26.518126512773254
43	22.44831238552632	24.27778818688987	26.990360312477296	26.283539115106514
44	21.702297694657833	25.148648493631537	27.48438951832152	25.664664293389112
45	21.788141004806462	24.64525108690249	25.61495547236763	27.951652435923418
46	21.611101126860735	26.952505133391707	27.51345005984177	23.922943679905785
47	19.50325592777691	25.666576171120703	30.686019967651028	24.14414793345136
48	19.876072085437993	23.3526305525709	30.401723748962805	26.3695736130283
49	20.882102147803444	23.715122570481373	30.415298080857134	24.987477200858052
50	20.584805160540373	24.09042416919353	28.32160077698711	27.003169893278983
51	18.824730520833732	24.293847959835272	27.404281841367677	29.477139677963315
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	106.0
1	337.5
2	569.0
3	1091.0
4	1613.0
5	1145.5
6	678.0
7	661.5
8	645.0
9	735.5
10	826.0
11	851.0
12	876.0
13	891.5
14	907.0
15	842.5
16	778.0
17	879.5
18	981.0
19	1014.0
20	1047.0
21	1224.5
22	1402.0
23	1939.0
24	2476.0
25	2488.0
26	3818.0
27	5136.0
28	5654.0
29	6172.0
30	7667.0
31	9162.0
32	10081.5
33	11001.0
34	11658.5
35	12316.0
36	13695.5
37	15075.0
38	16768.0
39	18461.0
40	21304.5
41	24148.0
42	26797.5
43	29447.0
44	33261.5
45	37076.0
46	41933.0
47	46790.0
48	53240.0
49	59690.0
50	59524.5
51	59359.0
52	50157.0
53	40955.0
54	35593.5
55	30232.0
56	27144.0
57	24056.0
58	23360.5
59	22665.0
60	20838.0
61	19011.0
62	16668.0
63	14325.0
64	11139.5
65	7954.0
66	6855.0
67	5756.0
68	4600.0
69	3444.0
70	2958.0
71	2472.0
72	1865.0
73	1258.0
74	1058.5
75	656.5
76	454.0
77	358.5
78	263.0
79	152.5
80	42.0
81	37.5
82	33.0
83	19.0
84	5.0
85	13.0
86	21.0
87	11.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	523046.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.772078244848686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.6625500480978	20.442268125228065
2	13.143520516149861	7.826198424391754
3	5.230026019706338	4.67126231643875
4	2.545296707585785	3.03115090938399
5	1.485858076720934	2.2118541460438013
6	0.9000746768530864	1.6078256223286194
7	0.663545626832739	1.3828592624764033
8	0.48655311143685337	1.1588557843178067
9	0.3847867447693164	1.0310310965567437
>10	6.103644194933473	44.49901982243294
>50	0.3260765975125835	5.927077606580133
>100	0.06288157049447435	3.1988076929814113
>500	0.0025930544533803853	0.6093058939750401
>1k	0.0025930544533803853	2.402483296864523
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	4705	0.899538472715593	RNA PCR Primer, Index 36 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	2968	0.5674453107374877	RNA PCR Primer, Index 36 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2468	0.47185142415772224	RNA PCR Primer, Index 15 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2307	0.44107019267903785	RNA PCR Primer, Index 15 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG	881	0.16843642815354673	RNA PCR Primer, Index 36 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG	806	0.1540973451665819	RNA PCR Primer, Index 36 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	759	0.14511151982808396	No Hit
GCCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG	711	0.13593450671642646	RNA PCR Primer, Index 15 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.911877731595309E-4	0.0	0.0	0.6022414854525223	0.0
2	1.911877731595309E-4	0.0	0.0	2.5686077323982976	0.0
3	1.911877731595309E-4	0.0	0.0	3.5188109650011663	0.0
4	1.911877731595309E-4	0.0	0.0	4.3458892716892965	0.0
5	1.911877731595309E-4	0.0	0.0	5.706190277719359	0.0
6	1.911877731595309E-4	0.0	0.0	6.482030261200736	0.0
7	1.911877731595309E-4	0.0	0.0	7.245443039426743	0.0
8	1.911877731595309E-4	0.0	0.0	8.401555503722426	0.0
9	1.911877731595309E-4	0.0	0.0	8.947587783866046	0.0
10	1.911877731595309E-4	0.0	0.0	10.09146423067952	0.0
11	3.823755463190618E-4	0.0	0.0	11.94617681810013	0.0
12	3.823755463190618E-4	0.0	0.0	13.709310462177323	0.0
13	3.823755463190618E-4	0.0	0.0	14.327229345028927	0.0
14	3.823755463190618E-4	0.0	0.0	14.557037048366682	0.0
15	3.823755463190618E-4	0.0	0.0	14.93673596586151	0.0
16	3.823755463190618E-4	0.0	0.0	15.739151049812062	0.0
17	3.823755463190618E-4	0.0	0.0	16.8241416624924	0.0
18	3.823755463190618E-4	0.0	0.0	18.016197428142075	0.0
19	3.823755463190618E-4	0.0	0.0	18.83428990949171	0.0
20	5.735633194785927E-4	0.0	0.0	19.77875750889979	0.0
21	5.735633194785927E-4	0.0	0.0	20.933722846556517	0.0
22	5.735633194785927E-4	0.0	0.0	22.132470184266776	0.0
23	5.735633194785927E-4	0.0	0.0	23.220710989090826	0.0
24	5.735633194785927E-4	0.0	0.0	24.069967077465463	0.0
25	5.735633194785927E-4	0.0	0.0	24.847527750905275	0.0
26	5.735633194785927E-4	0.0	0.0	25.50903744603725	0.0
27	5.735633194785927E-4	0.0	0.0	26.189665918485183	0.0
28	5.735633194785927E-4	0.0	0.0	26.893619299258575	0.0
29	5.735633194785927E-4	0.0	0.0	27.59967574553672	0.0
30	5.735633194785927E-4	0.0	0.0	28.39100193864402	0.0
31	5.735633194785927E-4	0.0	0.0	29.16053272561113	0.0
32	5.735633194785927E-4	0.0	0.0	29.859897599828695	0.0
33	5.735633194785927E-4	0.0	0.0	30.545305766605615	0.0
34	5.735633194785927E-4	0.0	0.0	31.209300902788666	0.0
35	5.735633194785927E-4	0.0	0.0	31.902738956038284	0.0
36	7.647510926381236E-4	0.0	0.0	32.60917777786275	0.0
37	7.647510926381236E-4	0.0	0.0	33.32479361279887	0.0
38	7.647510926381236E-4	0.0	0.0	34.04021825996184	0.0
39	9.559388657976545E-4	0.0	0.0	34.74168619968416	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	125	0.0	45.000004	1
CACGGCG	25	3.8878796E-5	45.0	21
CGAAACT	20	7.029758E-4	45.0	8
CGAAACG	25	3.8878796E-5	45.0	14
TCTTACG	20	7.029758E-4	45.0	1
GCACGGG	55	1.8189894E-12	45.0	3
TAACGGA	20	7.029758E-4	45.0	22
TCTAGCG	20	7.029758E-4	45.0	1
CACTCGT	20	7.029758E-4	45.0	27
ATTTTCG	25	3.8878796E-5	45.0	14
GTTCGTA	20	7.029758E-4	45.0	30
GTTAACG	20	7.029758E-4	45.0	20
CTCGGTA	20	7.029758E-4	45.0	11
CGGATAT	25	3.8878796E-5	45.0	14
CGTAGAC	20	7.029758E-4	45.0	31
CGCTAGG	20	7.029758E-4	45.0	2
ACGGATA	25	3.8878796E-5	45.0	13
GTCGTGG	25	3.8878796E-5	45.0	2
CGCAGAC	20	7.029758E-4	45.0	37
CGAGAGT	20	7.029758E-4	45.0	14
>>END_MODULE