SRR2933959.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933959.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1581458
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC	16142	1.0207036797689222	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	13129	0.830183286562147	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG	13098	0.8282230701036638	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	7125	0.45053362150623033	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	5498	0.3476538738303515	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	4929	0.3116744168988364	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	4395	0.27790810758173784	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACTAGGGT	3277	0.20721384949837426	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	3161	0.19987884597630792	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTC	2887	0.18255306179487538	No Hit
GAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	2809	0.1776209042541756	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTA	2702	0.1708549958329592	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2563	0.16206563816427627	No Hit
TCCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	1859	0.11754975472001153	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1775	0.11223820044541177	No Hit
CGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	1597	0.10098276400637893	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	20	7.034093E-4	45.0	44
ACGCACG	25	3.891474E-5	45.0	45
TCGATAT	25	3.891474E-5	45.0	14
TACGCTT	35	1.21232E-7	45.0	23
GCCGATA	20	7.034093E-4	45.0	9
TACGACG	20	7.034093E-4	45.0	15
CGGTCGA	70	0.0	45.0	41
ACACGCG	175	0.0	42.42857	36
TACGGCT	1965	0.0	40.87786	7
TATCGCG	50	1.0822987E-9	40.5	1
TCGATTG	45	1.9290383E-8	40.0	1
CCGCTAG	45	1.9290383E-8	40.0	1
CGTTTTT	1735	0.0	39.42363	1
ACCGGTA	40	3.4593177E-7	39.375	41
CGCCCAT	35	6.24978E-6	38.571426	2
CGTTTGG	830	0.0	38.493977	2
TTGGGAT	4300	0.0	38.40698	5
TGATACC	1655	0.0	38.066463	4
TTGTGCG	350	0.0	37.92857	1
CCGATGA	690	0.0	37.826088	18