##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933957.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2175099
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47285939628495	34.0	31.0	34.0	31.0	34.0
2	32.69809741993353	34.0	31.0	34.0	31.0	34.0
3	32.75167705010209	34.0	31.0	34.0	31.0	34.0
4	36.112659239878276	37.0	35.0	37.0	35.0	37.0
5	36.14348496321317	37.0	35.0	37.0	35.0	37.0
6	35.77486725891557	37.0	35.0	37.0	35.0	37.0
7	36.08501406142893	37.0	35.0	37.0	35.0	37.0
8	36.10541037442434	37.0	37.0	37.0	35.0	37.0
9	37.96125325789769	39.0	38.0	39.0	35.0	39.0
10	37.62884218143634	39.0	37.0	39.0	35.0	39.0
11	37.57166179562402	39.0	37.0	39.0	35.0	39.0
12	37.390186377723495	39.0	37.0	39.0	35.0	39.0
13	37.32724763332612	39.0	37.0	39.0	35.0	39.0
14	38.49187508246751	40.0	38.0	41.0	34.0	41.0
15	38.58613424032653	40.0	38.0	41.0	34.0	41.0
16	38.67418034765314	40.0	38.0	41.0	35.0	41.0
17	38.66681884364804	40.0	38.0	41.0	35.0	41.0
18	38.60627171452886	40.0	38.0	41.0	35.0	41.0
19	38.61171974241172	40.0	38.0	41.0	35.0	41.0
20	38.54283506176041	40.0	38.0	41.0	34.0	41.0
21	38.477182417903734	40.0	38.0	41.0	34.0	41.0
22	38.495716286936826	40.0	38.0	41.0	34.0	41.0
23	38.45116383208305	40.0	38.0	41.0	34.0	41.0
24	38.41623162899712	40.0	37.0	41.0	34.0	41.0
25	38.329010771463736	40.0	37.0	41.0	34.0	41.0
26	38.28098307249463	40.0	37.0	41.0	34.0	41.0
27	38.27675567870704	40.0	37.0	41.0	34.0	41.0
28	38.200564204204035	40.0	37.0	41.0	34.0	41.0
29	38.168018559155236	40.0	37.0	41.0	34.0	41.0
30	38.07110940697412	40.0	37.0	41.0	34.0	41.0
31	38.00152820630233	40.0	37.0	41.0	34.0	41.0
32	37.91266098692519	40.0	37.0	41.0	34.0	41.0
33	37.82481073275285	40.0	37.0	41.0	33.0	41.0
34	37.76073778710762	40.0	37.0	41.0	33.0	41.0
35	37.71260158733005	40.0	37.0	41.0	33.0	41.0
36	37.633135319357876	40.0	36.0	41.0	33.0	41.0
37	37.574975667774204	40.0	36.0	41.0	33.0	41.0
38	37.520369877417075	40.0	36.0	41.0	33.0	41.0
39	37.43742376783769	40.0	36.0	41.0	33.0	41.0
40	37.33244279915535	40.0	36.0	41.0	32.0	41.0
41	37.24870913921619	40.0	35.0	41.0	32.0	41.0
42	37.2571551915568	40.0	35.0	41.0	32.0	41.0
43	37.181600009930584	40.0	35.0	41.0	32.0	41.0
44	37.08754038321934	39.0	35.0	41.0	32.0	41.0
45	37.07364676274505	39.0	35.0	41.0	32.0	41.0
46	37.008416628392546	39.0	35.0	41.0	32.0	41.0
47	36.94716792201183	39.0	35.0	41.0	32.0	41.0
48	36.877556377893605	39.0	35.0	41.0	32.0	41.0
49	36.83758900169602	39.0	35.0	41.0	31.0	41.0
50	36.75277631041162	39.0	35.0	41.0	31.0	41.0
51	35.89731915650736	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	27.0
10	34.0
11	54.0
12	32.0
13	39.0
14	37.0
15	62.0
16	95.0
17	176.0
18	301.0
19	569.0
20	958.0
21	1651.0
22	2415.0
23	3731.0
24	5326.0
25	8374.0
26	11869.0
27	14326.0
28	16930.0
29	19889.0
30	24533.0
31	32177.0
32	43217.0
33	63482.0
34	121013.0
35	139935.0
36	146315.0
37	238221.0
38	463495.0
39	815295.0
40	507.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.480678350732543	4.60608919410105	55.735945812121656	16.177286643044752
2	19.64811716616117	4.317918402794539	54.11565174734575	21.918312683698534
3	20.786732006221325	4.582366135978178	52.00112730501002	22.629774552790472
4	19.4033007233234	4.946349568456424	51.00232219315075	24.64802751506943
5	18.682919719975967	5.639053670660508	50.465565015661355	25.212461593702173
6	21.955782242555397	6.66397253642248	52.805550460002046	18.574694761020073
7	84.9202725944888	2.227622742688953	8.407617308453547	4.4444873543686985
8	86.9531915558786	2.628707934673318	6.503979818849624	3.914120690598451
9	81.20540720215493	5.152179280115526	8.666318176781838	4.976095340947699
10	37.51626937440549	31.214257374032172	15.90378185084909	15.365691400713255
11	28.791976824962912	25.165291326969484	25.516309832334066	20.526422015733537
12	26.284918525547575	22.659612275119432	29.32441236008108	21.731056839251913
13	25.394752146913774	21.903738634425377	31.589320761951523	21.112188456709326
14	21.06354699257367	24.040928711750592	32.451994139117346	22.443530156558392
15	19.69993089969698	24.86015579060999	33.51718703378559	21.922726275907443
16	23.784618539202125	25.003597537399447	30.14727145752906	21.06451246586937
17	23.928152235829266	24.731380042931377	28.92489031533737	22.415577405901985
18	23.48725276412706	24.11379895811639	30.501692106888008	21.897256170868545
19	24.361235971328203	25.207082528197567	29.178396017836427	21.2532854826378
20	24.457691351060344	27.740484456109815	28.773173083156216	19.02865110967363
21	25.1346720310202	25.241425792573118	30.025621822271077	19.598280354135607
22	23.842179137593277	23.400865891621482	30.55428741404414	22.202667556741098
23	22.542422206989198	24.14331485601345	30.171959988947627	23.14230294804972
24	22.39594611555612	24.87408619101935	29.409465959940214	23.320501733484313
25	22.403669901921706	26.36776532930225	27.383121411944927	23.845443356831115
26	20.662737650102365	26.47907060782061	27.686142102037653	25.172049640039372
27	20.75197496757619	25.06681305080826	30.176971255101492	24.004240726514055
28	19.719102440854417	25.87477627455118	29.32606745715942	25.080053827434984
29	20.780801241690607	25.726507161283234	29.103548849960394	24.389142747065765
30	22.487114379621342	25.36551209852977	29.017437826967875	23.129935694881016
31	22.501550504137974	26.56660685329725	27.630788299750954	23.30105434281382
32	22.65147471448426	26.904016782684376	26.756988992225182	23.687519510606183
33	22.390934849402257	26.31015875599226	27.09918031317195	24.199726081433536
34	20.794042018317327	26.027688854622248	29.193475791216862	23.984793335843563
35	20.005756059839115	26.871926289332116	27.442980756278217	25.67933689455055
36	21.985252165533613	26.621500906395525	26.588169090234516	24.805077837836347
37	21.470930748439496	27.63644321476861	27.451807940696032	23.440818096095857
38	21.33958040530569	27.616260225396637	27.60297347385108	23.441185895446598
39	20.574006056735808	27.24749540135874	28.135454983888092	24.04304355801736
40	21.755791345589326	26.556538346070685	28.312596346189302	23.37507396215069
41	19.934127136282072	25.62687951215094	28.078721934036103	26.36027141753088
42	20.399761114321695	24.38974042101072	29.678787034521186	25.531711430146398
43	20.674553204244955	23.86728144328143	30.21421093936414	25.24395441310947
44	21.3019729216923	24.42380783587322	28.446153485427562	25.828065757006925
45	21.160232246900026	24.1076842939103	27.419763422262616	27.31232003692706
46	21.16630093618727	24.635752211738406	28.447992482181274	25.749954369893047
47	19.225423762320702	25.49759804036506	30.143501514183953	25.133476683130283
48	19.593774812089013	25.82038794556018	29.46325661498626	25.12258062736455
49	21.72420657634434	24.537871609522142	29.63607633491625	24.101845479217268
50	20.413829439487582	25.008838678147523	28.62904171258412	25.94829016978078
51	19.2200446968161	25.001206841619624	27.746828994910118	28.03191946665416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	252.0
1	1213.0
2	2174.0
3	6065.5
4	9957.0
5	6697.5
6	3438.0
7	3424.0
8	3410.0
9	3467.5
10	3525.0
11	3532.5
12	3540.0
13	3506.5
14	3473.0
15	3493.5
16	3514.0
17	3453.5
18	3393.0
19	3886.5
20	4380.0
21	5388.5
22	6397.0
23	7245.5
24	8094.0
25	10461.0
26	15631.0
27	18434.0
28	22691.5
29	26949.0
30	30787.5
31	34626.0
32	41849.0
33	49072.0
34	54808.5
35	60545.0
36	66103.5
37	71662.0
38	80156.5
39	88651.0
40	95956.0
41	103261.0
42	111305.5
43	119350.0
44	133124.5
45	146899.0
46	166309.5
47	185720.0
48	206412.5
49	227105.0
50	225165.5
51	223226.0
52	199242.0
53	175258.0
54	156435.5
55	137613.0
56	124112.5
57	110612.0
58	102629.5
59	94647.0
60	83504.0
61	72361.0
62	64703.5
63	57046.0
64	48278.5
65	39511.0
66	32139.0
67	24767.0
68	20387.0
69	16007.0
70	12989.5
71	9972.0
72	8219.0
73	6466.0
74	5066.5
75	2664.0
76	1661.0
77	1262.5
78	864.0
79	657.5
80	451.0
81	323.5
82	196.0
83	128.5
84	61.0
85	37.0
86	13.0
87	16.5
88	20.0
89	15.5
90	11.0
91	8.5
92	6.0
93	3.5
94	1.0
95	5.0
96	9.0
97	6.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2175099.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.86472677179097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17110305210714	18.85127510770046
2	11.682782497345386	6.277095194508911
3	4.366865618137534	3.5194395504117875
4	2.1543249504955795	2.3150140469086344
5	1.2794666133757164	1.718626049098367
6	0.8650576132275899	1.39437218527301
7	0.6065024334401765	1.1405465512557678
8	0.5002425985478665	1.075110458367932
9	0.4356564105082802	1.053341139121574
>10	7.382987762674055	49.80418057335271
>50	0.4917008644285006	7.861165097456799
>100	0.057917978699122075	2.7685337413059736
>500	0.003130610523734456	0.5493327378196796
>1k	0.0019131508756155006	1.1677701043061368
>5k	3.4784561374827285E-4	0.5041974631123723
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5596	0.2572756458441662	No Hit
CCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	5195	0.2388397033882136	No Hit
GCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	4978	0.22886314599933152	No Hit
CTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGCT	4903	0.22541502708612343	No Hit
TCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	4299	0.19764617610508764	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3792475652832354E-4	0.0	0.0	0.23893165322589915	0.0
2	1.3792475652832354E-4	0.0	0.0	0.9641400230518243	0.0
3	1.3792475652832354E-4	0.0	0.0	1.4475662946835983	0.0
4	1.3792475652832354E-4	0.0	0.0	1.974071065271052	0.0
5	1.3792475652832354E-4	0.0	0.0	2.873294502916879	0.0
6	1.3792475652832354E-4	0.0	0.0	3.5989626219312316	0.0
7	1.3792475652832354E-4	0.0	0.0	4.206888973789239	0.0
8	1.3792475652832354E-4	0.0	0.0	5.133283588471145	0.0
9	1.3792475652832354E-4	0.0	0.0	5.623330248416279	0.0
10	1.3792475652832354E-4	0.0	0.0	6.471659450903155	0.0
11	1.3792475652832354E-4	0.0	0.0	7.993153415085934	0.0
12	1.8389967537109806E-4	0.0	0.0	9.373872177772139	0.0
13	1.8389967537109806E-4	0.0	0.0	9.83771313397689	0.0
14	1.8389967537109806E-4	0.0	0.0	10.025336777774253	0.0
15	1.8389967537109806E-4	0.0	0.0	10.274566812820934	0.0
16	2.2987459421387255E-4	0.0	0.0	10.793577671637015	0.0
17	2.2987459421387255E-4	0.0	0.0	11.512947226769908	0.0
18	2.758495130566471E-4	0.0	0.0	12.250660774521068	0.0
19	2.758495130566471E-4	0.0	0.0	12.836335265659173	0.0
20	3.677993507421961E-4	0.0	0.0	13.494236354299275	0.0
21	4.137742695849706E-4	0.0	0.0	14.395436713455341	0.0
22	4.137742695849706E-4	0.0	0.0	15.391115530833309	0.0
23	4.137742695849706E-4	0.0	0.0	16.287028774322454	0.0
24	4.137742695849706E-4	0.0	0.0	16.982629296413634	0.0
25	4.137742695849706E-4	0.0	0.0	17.610508763049406	0.0
26	4.137742695849706E-4	0.0	0.0	18.167908679099206	0.0
27	4.137742695849706E-4	0.0	0.0	18.77762805279208	0.0
28	4.137742695849706E-4	0.0	0.0	19.356452281022612	0.0
29	4.137742695849706E-4	0.0	0.0	19.987871816409275	0.0
30	4.137742695849706E-4	0.0	0.0	20.695287892643048	0.0
31	4.597491884277451E-4	0.0	0.0	21.333235866505387	0.0
32	4.597491884277451E-4	0.0	0.0	21.953437521694415	0.0
33	4.597491884277451E-4	0.0	0.0	22.55437568588832	0.0
34	4.597491884277451E-4	0.0	0.0	23.1933351079652	0.0
35	4.597491884277451E-4	0.0	0.0	23.842041212836747	0.0
36	4.597491884277451E-4	0.0	0.0	24.435071690989698	0.0
37	4.597491884277451E-4	0.0	0.0	25.048790882621894	0.0
38	4.597491884277451E-4	0.0	0.0	25.73275975024585	0.0
39	4.597491884277451E-4	0.0	0.0	26.718737859747993	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	3045	0.0	40.714283	1
CGTTTGG	1275	0.0	38.999996	2
TGGGCGA	2505	0.0	38.622753	6
TTCGTTG	750	0.0	38.4	1
TACGGGA	510	0.0	38.382355	4
CCATACG	170	0.0	38.382355	2
TTTTGCG	1845	0.0	37.804874	1
TTCTTCG	645	0.0	37.67442	1
TCGTTTG	685	0.0	37.445255	1
CGAATAG	115	0.0	37.173912	26
GGGCGAT	3755	0.0	37.090546	7
TTTGGGA	13320	0.0	36.976353	4
CGGGTAT	305	0.0	36.885246	6
TTTGGGC	5660	0.0	36.572437	4
CTACGGG	335	0.0	36.268654	3
TTGGGAT	6805	0.0	35.97355	5
TTGGGAG	6950	0.0	35.870502	5
TGCGGGA	3180	0.0	35.801888	4
TTGGGAC	5370	0.0	35.782124	5
TTGGGAA	5595	0.0	35.670242	5
>>END_MODULE