##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933956.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	540161
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.361190459881406	33.0	31.0	34.0	31.0	34.0
2	32.59116818874373	34.0	31.0	34.0	31.0	34.0
3	32.65440859299357	34.0	31.0	34.0	31.0	34.0
4	36.034624861846744	37.0	35.0	37.0	35.0	37.0
5	36.07694742863702	37.0	35.0	37.0	35.0	37.0
6	35.716530812109724	37.0	35.0	37.0	35.0	37.0
7	36.04029354211059	37.0	35.0	37.0	35.0	37.0
8	36.04346296752264	37.0	36.0	37.0	35.0	37.0
9	37.89937814836688	39.0	38.0	39.0	35.0	39.0
10	37.5816913846057	39.0	37.0	39.0	35.0	39.0
11	37.53003641506884	39.0	37.0	39.0	35.0	39.0
12	37.3170961990962	39.0	37.0	39.0	35.0	39.0
13	37.233813622234855	39.0	37.0	39.0	34.0	39.0
14	38.33731794779705	40.0	38.0	41.0	34.0	41.0
15	38.43402615146225	40.0	38.0	41.0	34.0	41.0
16	38.539398438613674	40.0	38.0	41.0	34.0	41.0
17	38.53159335827652	40.0	38.0	41.0	34.0	41.0
18	38.52425665681158	40.0	38.0	41.0	35.0	41.0
19	38.54933251382458	40.0	38.0	41.0	35.0	41.0
20	38.49847175194063	40.0	38.0	41.0	34.0	41.0
21	38.394389820812684	40.0	37.0	41.0	34.0	41.0
22	38.42129291081733	40.0	37.0	41.0	34.0	41.0
23	38.38878038214532	40.0	37.0	41.0	34.0	41.0
24	38.36736824761506	40.0	37.0	41.0	34.0	41.0
25	38.264930270789634	40.0	37.0	41.0	34.0	41.0
26	38.17880594859681	40.0	37.0	41.0	34.0	41.0
27	38.150903156651445	40.0	37.0	41.0	34.0	41.0
28	38.04133212134901	40.0	37.0	41.0	34.0	41.0
29	38.01596931285302	40.0	37.0	41.0	34.0	41.0
30	37.951297853788034	40.0	36.0	41.0	34.0	41.0
31	37.834275336427474	40.0	36.0	41.0	33.0	41.0
32	37.73937400145512	40.0	36.0	41.0	33.0	41.0
33	37.71865610438369	40.0	36.0	41.0	33.0	41.0
34	37.72002051240278	40.0	36.0	41.0	33.0	41.0
35	37.705535942061715	40.0	36.0	41.0	33.0	41.0
36	37.609418303061496	40.0	36.0	41.0	33.0	41.0
37	37.475358272811256	40.0	36.0	41.0	33.0	41.0
38	37.37709312593838	40.0	35.0	41.0	33.0	41.0
39	37.380732781522546	40.0	35.0	41.0	33.0	41.0
40	37.31223283428459	39.0	35.0	41.0	33.0	41.0
41	37.23532058034549	39.0	35.0	41.0	32.0	41.0
42	37.226121471191	39.0	35.0	41.0	32.0	41.0
43	37.11086694522559	39.0	35.0	41.0	32.0	41.0
44	36.97298027810227	39.0	35.0	41.0	32.0	41.0
45	36.97457980120742	39.0	35.0	41.0	32.0	41.0
46	36.877532809662306	39.0	35.0	41.0	32.0	41.0
47	36.78675987344514	39.0	35.0	41.0	31.0	41.0
48	36.739920134922734	39.0	35.0	41.0	31.0	41.0
49	36.74035889299672	39.0	35.0	41.0	31.0	41.0
50	36.67208110174559	39.0	35.0	40.0	31.0	41.0
51	35.65360327754133	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	9.0
11	9.0
12	10.0
13	12.0
14	9.0
15	13.0
16	15.0
17	37.0
18	52.0
19	125.0
20	226.0
21	371.0
22	583.0
23	886.0
24	1274.0
25	1896.0
26	2618.0
27	3282.0
28	4075.0
29	4975.0
30	6366.0
31	8370.0
32	11083.0
33	16420.0
34	31346.0
35	41677.0
36	38463.0
37	60747.0
38	120962.0
39	184125.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.71311516381227	4.905944709077479	54.95361568125059	16.42732444585966
2	19.931464878064133	4.587335997970976	52.924405871582735	22.55679325238216
3	21.670020604967778	4.507359842713561	51.712544963446085	22.11007458887258
4	20.830456104753953	4.634914405149576	49.74109571035302	24.793533779743445
5	18.408770718359897	5.5611197402256	49.637052656522776	26.393056884891724
6	21.75814247974215	6.3775429918117	53.06232771340397	18.80198681504218
7	81.86947965513986	2.314680252739461	10.036452094838392	5.779387997282292
8	82.35007710664043	2.836191431813848	8.539861263586227	6.273870197959496
9	76.22116369008499	5.452818696647851	11.481761919131518	6.8442556941356365
10	35.62067605769391	30.309296672658707	18.983229074294517	15.086798195352866
11	29.188889979098825	25.893205914532892	25.928010352468988	18.989893753899302
12	27.02897839718158	22.42442531023158	30.588657826092593	19.957938466494248
13	24.371807664751806	24.76817097124746	30.7611989758609	20.098822388139833
14	18.798284215261745	27.071373164667573	31.338619411619867	22.791723208450815
15	16.798695203837376	25.961889140459977	36.00870851468358	21.230707141019067
16	19.184280242372182	25.589777862526176	32.4331079067167	22.792833988384945
17	20.291357576722497	25.083262212562552	28.837142999957422	25.78823721075753
18	20.957085017244857	24.358478305542235	31.463026764242514	23.221409912970394
19	22.82856407626615	25.336520037544364	28.98191465137246	22.85300123481703
20	25.153981868368874	26.268834662258104	28.91063960559907	19.66654386377395
21	23.42338673099317	26.332889638459644	30.261533135491085	19.982190495056106
22	21.10704030835251	24.621733149931224	29.348286899646585	24.92293964206968
23	20.457419176875042	25.253952062440643	29.605987844364922	24.682640916319393
24	22.300758477565022	23.746623691825214	29.453810993389006	24.498806837220755
25	21.6846458741005	25.686971106762613	26.783310901749662	25.845072117387225
26	21.793872567623357	27.365174457245157	27.076927064338225	23.76402591079326
27	22.453120458529956	26.21773878528809	29.65560268142276	21.67353807475919
28	19.03636138114377	25.46462998994744	32.533818620744555	22.96519000816423
29	21.31882901579344	25.479440389069186	30.244871436479126	22.95685915865825
30	23.516877375449173	26.497285068710998	28.17363711930332	21.812200436536514
31	22.933347650052486	29.235912996310358	26.332149118503555	21.498590235133598
32	23.506510096063952	30.03067603918091	25.109735800992667	21.35307806376247
33	23.072195141818828	27.470513420998554	25.649945108958256	23.807346328224362
34	21.283284057901255	25.71770268494023	28.85898833866199	24.140024918496522
35	21.849596694318915	25.562563754139973	26.21181462563939	26.37602492590172
36	25.05123472446178	25.888762794796367	26.426935672882713	22.63306680785914
37	22.79635145817636	27.141907690484874	28.980989001427353	21.080751849911415
38	19.360523991920928	30.052891637863528	27.953517562356407	22.63306680785914
39	21.10722543834153	27.327222809495687	28.272311403451933	23.293240348710846
40	24.65116881818569	25.46462998994744	27.82226040013996	22.06194079172691
41	21.54505786237807	25.589407602548132	26.07296713387305	26.79256740120075
42	22.154505786237806	24.429938481304646	27.053970945699525	26.361584786758023
43	22.682866774906	23.99895586686192	28.257871264308232	25.060306093923852
44	21.67205703484702	24.15724200747555	28.364506137984787	25.806194819692646
45	22.540316683359222	24.045978884073453	26.27772090173115	27.135983530836178
46	23.116256079206014	25.235809323516506	28.12698436206983	23.520950235207653
47	20.315239345306306	24.798162029468994	31.10109763570491	23.785500989519793
48	19.383480110559628	25.1006644315306	30.276528664601848	25.23932679330792
49	21.606335888744283	23.112183219447534	31.413226797195655	23.86825409461253
50	21.870886643056423	22.934643559975637	28.66071412041965	26.53375567654829
51	19.438093457321056	23.711634123900097	26.942892952286446	29.907379466492394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	73.0
1	259.0
2	445.0
3	990.5
4	1536.0
5	1078.0
6	620.0
7	661.0
8	702.0
9	767.5
10	833.0
11	898.5
12	964.0
13	954.5
14	945.0
15	888.5
16	832.0
17	865.5
18	899.0
19	1076.0
20	1253.0
21	1173.5
22	1094.0
23	1661.5
24	2229.0
25	2381.5
26	3474.0
27	4414.0
28	5114.0
29	5814.0
30	6503.0
31	7192.0
32	8873.5
33	10555.0
34	11807.0
35	13059.0
36	14464.0
37	15869.0
38	17403.5
39	18938.0
40	22434.5
41	25931.0
42	29091.5
43	32252.0
44	35774.0
45	39296.0
46	47168.5
47	55041.0
48	63649.5
49	72258.0
50	69044.5
51	65831.0
52	53849.0
53	41867.0
54	35549.0
55	29231.0
56	26360.5
57	23490.0
58	21267.0
59	19044.0
60	17520.0
61	15996.0
62	13910.0
63	11824.0
64	9382.5
65	6941.0
66	5447.5
67	3954.0
68	3453.0
69	2952.0
70	2324.5
71	1697.0
72	1276.5
73	856.0
74	633.0
75	293.0
76	176.0
77	174.5
78	173.0
79	127.0
80	81.0
81	68.0
82	55.0
83	29.0
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	540161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.18440449307781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.89065634064731	19.52163971452911
2	13.425819001465708	7.8364906477925045
3	5.749094518691476	5.033516997072855
4	2.867685763715459	3.3476680514925086
5	1.7891851393807827	2.610815141034627
6	1.123013388795671	1.966468619385295
7	0.7766877172842707	1.5867017954220588
8	0.6335244894779412	1.479122796575588
9	0.4682076763352105	1.2297925991637755
>10	5.859838863711678	41.70199160152486
>50	0.33097710917594675	5.898018707388522
>100	0.0776128492447472	3.9646471642422796
>500	0.004489999543084549	0.9322415070554112
>1k	0.003207142530774678	2.8908846573206257
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	4041	0.7481102856370601	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	4000	0.7405199560871666	TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	3141	0.5814932955174477	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	3025	0.5600182167909198	TruSeq Adapter, Index 14 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG	1236	0.22882066643093446	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	856	0.15847127060265365	No Hit
GCCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG	852	0.15773075064656647	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG	841	0.15569432076732678	TruSeq Adapter, Index 15 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG	775	0.14347574149188852	TruSeq Adapter, Index 15 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	623	0.1153359831605762	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8512998902179165E-4	0.0	0.0	0.7805080337158736	0.0
2	1.8512998902179165E-4	0.0	0.0	2.8467438411880903	0.0
3	1.8512998902179165E-4	0.0	0.0	4.0319460309056	0.0
4	1.8512998902179165E-4	0.0	0.0	5.153648634388636	0.0
5	1.8512998902179165E-4	0.0	0.0	6.963479407065671	0.0
6	1.8512998902179165E-4	0.0	0.0	8.001503255510857	0.0
7	1.8512998902179165E-4	0.0	0.0	8.921969560927206	0.0
8	1.8512998902179165E-4	0.0	0.0	10.282674980237374	0.0
9	1.8512998902179165E-4	0.0	0.0	10.888420304316677	0.0
10	1.8512998902179165E-4	0.0	0.0	12.168779308391388	0.0
11	1.8512998902179165E-4	0.0	0.0	14.540294467760537	0.0
12	1.8512998902179165E-4	0.0	0.0	16.644667052971243	0.0
13	1.8512998902179165E-4	0.0	0.0	17.371487389870797	0.0
14	1.8512998902179165E-4	0.0	0.0	17.66436303250327	0.0
15	1.8512998902179165E-4	0.0	0.0	18.069797708460996	0.0
16	1.8512998902179165E-4	0.0	0.0	19.06505652944215	0.0
17	1.8512998902179165E-4	0.0	0.0	20.38262666131024	0.0
18	1.8512998902179165E-4	0.0	0.0	21.91920557019111	0.0
19	1.8512998902179165E-4	0.0	0.0	22.843744735365938	0.0
20	1.8512998902179165E-4	0.0	0.0	23.864366364843075	0.0
21	1.8512998902179165E-4	0.0	0.0	25.24154835317618	0.0
22	1.8512998902179165E-4	0.0	0.0	26.577816613935475	0.0
23	1.8512998902179165E-4	0.0	0.0	27.859286397944317	0.0
24	1.8512998902179165E-4	0.0	0.0	28.77308802375588	0.0
25	3.702599780435833E-4	0.0	0.0	29.605247324408833	0.0
26	3.702599780435833E-4	0.0	0.0	30.324292201769474	0.0
27	3.702599780435833E-4	0.0	0.0	31.05648130835066	0.0
28	3.702599780435833E-4	0.0	0.0	31.825696412736203	0.0
29	3.702599780435833E-4	0.0	0.0	32.675257932357205	0.0
30	3.702599780435833E-4	0.0	0.0	33.5944283278504	0.0
31	3.702599780435833E-4	0.0	0.0	34.4626879763626	0.0
32	3.702599780435833E-4	0.0	0.0	35.24023393025413	0.0
33	3.702599780435833E-4	0.0	0.0	35.965202967263465	0.0
34	3.702599780435833E-4	0.0	0.0	36.72571696216498	0.0
35	3.702599780435833E-4	0.0	0.0	37.52603390470619	0.0
36	3.702599780435833E-4	0.0	0.0	38.29062075936619	0.0
37	3.702599780435833E-4	0.0	0.0	39.01910726616694	0.0
38	3.702599780435833E-4	0.0	0.0	39.777955091167264	0.0
39	3.702599780435833E-4	0.0	0.0	40.73692843430015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	30	2.1633568E-6	45.000004	4
TCGATCA	30	2.1633568E-6	45.000004	17
CCTCGCG	30	2.1633568E-6	45.000004	1
GCCGATG	30	2.1633568E-6	45.000004	9
GCCGATC	20	7.029961E-4	45.000004	9
TCGTGCG	20	7.029961E-4	45.000004	1
TCTACGG	20	7.029961E-4	45.000004	2
ACGTGTA	20	7.029961E-4	45.000004	28
TCGCTAT	20	7.029961E-4	45.000004	27
TACCTAC	30	2.1633568E-6	45.000004	25
GCGATTA	20	7.029961E-4	45.000004	23
CGGCTAG	30	2.1633568E-6	45.000004	41
CCGCGAG	30	2.1633568E-6	45.000004	11
TTCGCTA	20	7.029961E-4	45.000004	26
TTACACG	20	7.029961E-4	45.000004	34
TACACGC	20	7.029961E-4	45.000004	35
CGCTTCA	20	7.029961E-4	45.000004	27
TCCGCGA	25	3.8880513E-5	45.0	10
ATTACGA	25	3.8880513E-5	45.0	36
TTCCGCG	45	3.8380676E-10	45.0	1
>>END_MODULE