FastQCFastQC Report
Sat 14 Jan 2017
SRR2933955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652333
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT67221.030455304269445TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC66611.0211042519694695TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC53620.8219728267617918TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC52450.8040372018585599TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG17510.26842118979110363No Hit
TCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG16840.25815036185506485No Hit
TGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG12280.1882474135142634No Hit
GCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG11740.17996943278969485No Hit
TTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT11040.16923871703562443No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC10480.16065414443236814No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.13949930480291506No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8800.1349004266225992No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC8790.13474713068325533No Hit
TTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG8560.13122132407834647No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC7680.11773128141608658No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG7430.11389888293249001No Hit
GAATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC7160.10975989257020571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.031042E-445.01
GTTAGCG253.8889448E-545.01
GTTACGG207.031042E-445.02
TGCGCGA207.031042E-445.014
GTTCGTC207.031042E-445.010
CATACGA502.1827873E-1145.018
GGTTCGT207.031042E-445.09
CAATACG207.031042E-445.031
TACACGA253.8889448E-545.018
AATCGTT207.031042E-445.022
GATAGCG207.031042E-445.01
TTACCGG302.1640535E-644.9999962
CGTTTTT6750.042.6666681
TACGGGA950.040.2631574
TCTCGTA451.9261279E-840.028
TTTTGCG4700.039.7340431
AGGCACG1250.039.610
ATGGGCG403.4551522E-739.3755
CGCATGG403.4551522E-739.3752
CGGGTAA750.039.06
Produced by FastQC (version 0.11.5)