##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933955.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	652333
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34001039346469	33.0	31.0	34.0	31.0	34.0
2	32.56161347042078	34.0	31.0	34.0	31.0	34.0
3	32.63882097027132	34.0	31.0	34.0	31.0	34.0
4	36.02075780314655	37.0	35.0	37.0	35.0	37.0
5	36.0516346712492	37.0	35.0	37.0	35.0	37.0
6	35.67971266209129	37.0	35.0	37.0	35.0	37.0
7	35.983115985240666	37.0	35.0	37.0	35.0	37.0
8	35.981901881401065	37.0	35.0	37.0	35.0	37.0
9	37.81908319830516	39.0	38.0	39.0	35.0	39.0
10	37.568617561889404	39.0	37.0	39.0	35.0	39.0
11	37.509727393831064	39.0	37.0	39.0	35.0	39.0
12	37.19909156826345	39.0	37.0	39.0	34.0	39.0
13	37.03421412070216	39.0	37.0	39.0	33.0	39.0
14	37.86888751603859	40.0	37.0	41.0	33.0	41.0
15	38.024740431650706	40.0	37.0	41.0	33.0	41.0
16	38.207706800054574	40.0	37.0	41.0	33.0	41.0
17	38.22647328894905	40.0	37.0	41.0	34.0	41.0
18	38.20705222639358	40.0	37.0	41.0	34.0	41.0
19	38.25555966048015	40.0	37.0	41.0	34.0	41.0
20	38.192889214557596	40.0	37.0	41.0	34.0	41.0
21	38.120124231029244	40.0	37.0	41.0	34.0	41.0
22	38.17716258414031	40.0	37.0	41.0	34.0	41.0
23	38.16339660878723	40.0	36.0	41.0	34.0	41.0
24	38.11713036133386	40.0	36.0	41.0	34.0	41.0
25	37.99589473474437	40.0	36.0	41.0	34.0	41.0
26	37.915357647091284	40.0	36.0	41.0	34.0	41.0
27	37.86340105436947	40.0	36.0	41.0	34.0	41.0
28	37.8127183509036	40.0	36.0	41.0	34.0	41.0
29	37.76463861248779	40.0	36.0	41.0	34.0	41.0
30	37.59835850708151	40.0	35.0	41.0	33.0	41.0
31	37.51904778694317	40.0	35.0	41.0	33.0	41.0
32	37.46287095701122	40.0	35.0	41.0	33.0	41.0
33	37.415215848347394	39.0	35.0	41.0	33.0	41.0
34	37.38994194682777	40.0	35.0	41.0	33.0	41.0
35	37.326175434938904	40.0	35.0	41.0	32.0	41.0
36	37.217701695299795	39.0	35.0	41.0	32.0	41.0
37	37.13571136214173	39.0	35.0	41.0	32.0	41.0
38	37.08890244706308	39.0	35.0	41.0	32.0	41.0
39	37.02136638802575	39.0	35.0	41.0	32.0	41.0
40	36.87177254561704	39.0	35.0	41.0	31.0	41.0
41	36.74790482774902	39.0	35.0	41.0	31.0	41.0
42	36.78704741290108	39.0	35.0	41.0	31.0	41.0
43	36.69602948187505	39.0	35.0	41.0	31.0	41.0
44	36.54363645561393	39.0	35.0	41.0	31.0	41.0
45	36.54130175845772	39.0	35.0	41.0	31.0	41.0
46	36.41766091857993	38.0	35.0	40.0	31.0	41.0
47	36.26026431285862	38.0	35.0	40.0	31.0	41.0
48	36.17486774392833	38.0	35.0	40.0	31.0	41.0
49	36.13297809554323	38.0	35.0	40.0	31.0	41.0
50	35.992612668683016	38.0	35.0	40.0	30.0	41.0
51	34.95878332078862	36.0	34.0	39.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	16.0
10	23.0
11	25.0
12	17.0
13	10.0
14	14.0
15	20.0
16	42.0
17	66.0
18	109.0
19	233.0
20	354.0
21	625.0
22	923.0
23	1413.0
24	1905.0
25	2721.0
26	3569.0
27	4554.0
28	5461.0
29	6911.0
30	8950.0
31	11794.0
32	16054.0
33	24774.0
34	48460.0
35	50408.0
36	47516.0
37	76152.0
38	143940.0
39	195152.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.960973919761837	4.721361635851628	52.35316931689796	16.96449512748857
2	20.64589711083143	4.884621811252841	50.71228958216126	23.75719149575447
3	22.18928062814544	5.02979306581148	49.762161350107995	23.01876495593508
4	20.758722922188515	4.626164857519089	49.15326987903417	25.461842341258222
5	18.72816490963971	5.650641620154124	49.27483355893386	26.34635991127231
6	20.976556451996142	6.707463825990713	52.331401293511135	19.984578428502004
7	78.86493554672231	2.41410445278715	12.151769111787997	6.569190888702549
8	78.60203301074758	3.442107022027094	10.158002124681719	7.797857842543608
9	72.29068589202141	5.916456778976382	13.64717100008738	8.145686328914833
10	37.19266080360797	29.457807592134692	19.697608430050295	13.65192317420704
11	31.273138105844716	23.750293178483993	26.218511097859533	18.75805761781176
12	29.183407860709178	20.868329518819376	30.867670346280196	19.08059227419125
13	27.492093761928338	22.36618414214826	30.321783506276702	19.8199385896467
14	19.50368906678031	25.04196476339538	33.242071150777285	22.21227501904702
15	17.741858835901294	25.161995483901627	35.625976303513696	21.470169376683383
16	23.904662189403265	25.871755683063714	27.26690202703221	22.95668010050082
17	23.469455017606037	25.57052916225302	27.326074259618938	23.633941560522004
18	24.319021113449725	23.37272527988006	30.802366276119713	21.505887330550504
19	23.51636357504526	25.087646953319855	28.32893629480649	23.0670531768284
20	23.242730323316465	28.74084248382345	29.44431754947243	18.57210964338766
21	24.172470195436993	26.911408743693787	30.898636126027657	18.01748493484156
22	20.863730640639062	24.445950151226445	31.448661956393437	23.241657251741056
23	21.341707379513224	24.339869361200492	30.814323359388535	23.504099899897753
24	24.05213288305206	24.605377928144062	27.679881287624575	23.662607901179307
25	23.289025696998312	25.602261421697204	24.810181303107463	26.298531578197025
26	21.954584545009986	25.433942480297638	26.419175482460645	26.19229749223173
27	20.839356586283387	26.835220661839887	28.26961076628041	24.055811985596314
28	18.84375004790498	25.816722440839264	26.86787269692013	28.471654814335622
29	23.99480020173746	27.45453625678909	24.95765199675626	23.59301154471719
30	24.375434019128267	27.609825043344426	26.762405090651555	21.252335846875752
31	22.781922729648816	27.421730925769506	27.522906245736458	22.27344009884522
32	25.64549087659217	28.59337179017465	23.798887991256	21.96224934197718
33	23.672725433176	27.045389394680324	24.70486699277823	24.57701817936545
34	19.849984593758098	24.13935827253872	31.109264746686126	24.90139238701706
35	21.27716978904946	27.92500149463541	26.144929046974475	24.65289966934066
36	23.581361053327058	28.460924098581554	24.5006768015722	23.457038046519184
37	20.69295896421	29.090510521466793	27.25264550467323	22.963885009649978
38	22.173951034211054	29.360464670651336	25.053155366967484	23.41242892817012
39	22.91912259536157	25.54232270941375	27.558624199603578	23.9799304956211
40	24.962710762754607	24.74027835476666	27.976815522133634	22.3201953603451
41	19.474869430183663	25.69914445536252	25.817182328657296	29.008803785796516
42	20.67364367585267	24.749935998945325	27.084326563273663	27.492093761928338
43	22.861176730289593	22.450803500666073	27.554638505180634	27.133381263863704
44	22.86424264907647	21.969300955187	27.189794169542242	27.97666222619429
45	22.48452860732172	22.094390441691587	25.601035054182447	29.82004589680424
46	21.723107676600755	23.895157841163943	29.091890184920892	25.289844297314406
47	17.53996808378543	24.77798915584525	32.670430592964024	25.011612167405296
48	17.97655491903675	25.808751051993383	29.85745010600414	26.357243922965722
49	22.912684165909127	21.67604582322219	31.471349755416327	23.939920255452353
50	21.405938378098302	22.37814122541708	28.73593701376444	27.479983382720174
51	19.119069554966558	22.665417815747478	26.79689667700392	31.418615952282043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	343.0
2	567.0
3	1118.5
4	1670.0
5	1176.5
6	683.0
7	781.0
8	879.0
9	1078.5
10	1278.0
11	1320.5
12	1363.0
13	1303.5
14	1244.0
15	1234.0
16	1224.0
17	1211.0
18	1198.0
19	1224.5
20	1251.0
21	1513.0
22	1775.0
23	2001.0
24	2227.0
25	2722.0
26	3983.5
27	4750.0
28	5502.5
29	6255.0
30	7464.0
31	8673.0
32	10432.0
33	12191.0
34	13716.0
35	15241.0
36	16632.5
37	18024.0
38	18966.5
39	19909.0
40	22125.0
41	24341.0
42	28057.5
43	31774.0
44	36987.0
45	42200.0
46	51642.5
47	61085.0
48	73647.5
49	86210.0
50	83451.5
51	80693.0
52	66619.0
53	52545.0
54	47012.0
55	41479.0
56	38059.0
57	34639.0
58	31195.5
59	27752.0
60	23964.0
61	20176.0
62	17822.5
63	15469.0
64	13393.5
65	11318.0
66	9264.0
67	7210.0
68	5985.0
69	4760.0
70	3939.0
71	3118.0
72	2386.5
73	1655.0
74	1338.0
75	854.5
76	688.0
77	485.5
78	283.0
79	191.5
80	100.0
81	76.5
82	53.0
83	33.5
84	14.0
85	9.0
86	4.0
87	4.5
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	652333.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.235328833597706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8513126762154	23.161506912434508
2	11.271357468704814	7.266718288094543
3	3.9307929901657124	3.801312138443775
4	1.9668337571394305	2.5360613168963995
5	1.1750210734880255	1.8938595345146736
6	0.8674845219067778	1.6778189293032764
7	0.7169913777402601	1.6178716982618084
8	0.6092613402911918	1.5711791719908028
9	0.5752832424401076	1.669000004326366
>10	6.851533747509729	41.938757369541186
>50	0.10528456700660987	2.3236473188318345
>100	0.067305202651698	4.068164983727635
>500	0.0067305202651698	1.5182588223526583
>1k	0.002884508685072771	1.238069789912768
>5k	0.001923005790048514	3.7177737213677937
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	6722	1.030455304269445	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	6661	1.0211042519694695	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	5362	0.8219728267617918	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	5245	0.8040372018585599	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	1751	0.26842118979110363	No Hit
TCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	1684	0.25815036185506485	No Hit
TGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	1228	0.1882474135142634	No Hit
GCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	1174	0.17996943278969485	No Hit
TTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	1104	0.16923871703562443	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	1048	0.16065414443236814	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	910	0.13949930480291506	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	880	0.1349004266225992	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC	879	0.13474713068325533	No Hit
TTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	856	0.13122132407834647	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC	768	0.11773128141608658	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG	743	0.11389888293249001	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	716	0.10975989257020571	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1141548871511942	0.0
2	0.0	0.0	0.0	4.037048562620625	0.0
3	0.0	0.0	0.0	5.545020717946202	0.0
4	0.0	0.0	0.0	7.02800563515873	0.0
5	0.0	0.0	0.0	9.405778950321386	0.0
6	0.0	0.0	0.0	10.620036085864122	0.0
7	0.0	0.0	0.0	11.677778067336774	0.0
8	0.0	0.0	0.0	13.107415997657638	0.0
9	0.0	0.0	0.0	13.7615297708379	0.0
10	0.0	0.0	0.0	15.125250447240903	0.0
11	0.0	0.0	0.0	17.373488693657993	0.0
12	0.0	0.0	0.0	19.43823170068048	0.0
13	0.0	0.0	0.0	20.16286160595892	0.0
14	0.0	0.0	0.0	20.488615477064627	0.0
15	0.0	0.0	0.0	20.94421100879459	0.0
16	0.0	0.0	0.0	21.859694358556137	0.0
17	0.0	0.0	0.0	23.132357246988885	0.0
18	0.0	0.0	0.0	24.49776417872467	0.0
19	0.0	0.0	0.0	25.375076839589596	0.0
20	0.0	0.0	0.0	26.327351214793673	0.0
21	0.0	0.0	0.0	27.400269494261366	0.0
22	0.0	0.0	0.0	28.475640508758563	0.0
23	0.0	0.0	0.0	29.382999173734888	0.0
24	0.0	0.0	0.0	30.076203411447835	0.0
25	0.0	0.0	0.0	30.735835838444476	0.0
26	0.0	0.0	0.0	31.319586775465904	0.0
27	0.0	0.0	0.0	32.012791013178855	0.0
28	0.0	0.0	0.0	32.61340450352811	0.0
29	0.0	0.0	0.0	33.285607197550945	0.0
30	0.0	0.0	0.0	33.99030863071468	0.0
31	0.0	0.0	0.0	34.56271566822466	0.0
32	0.0	0.0	0.0	35.11074865137898	0.0
33	0.0	0.0	0.0	35.689287526462714	0.0
34	0.0	0.0	0.0	36.28990101681197	0.0
35	0.0	0.0	0.0	36.87150581068258	0.0
36	0.0	0.0	0.0	37.366498398823914	0.0
37	0.0	0.0	0.0	37.92832801651917	0.0
38	0.0	0.0	0.0	38.52556899620286	0.0
39	0.0	0.0	0.0	39.45484898050535	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	7.031042E-4	45.0	1
GTTAGCG	25	3.8889448E-5	45.0	1
GTTACGG	20	7.031042E-4	45.0	2
TGCGCGA	20	7.031042E-4	45.0	14
GTTCGTC	20	7.031042E-4	45.0	10
CATACGA	50	2.1827873E-11	45.0	18
GGTTCGT	20	7.031042E-4	45.0	9
CAATACG	20	7.031042E-4	45.0	31
TACACGA	25	3.8889448E-5	45.0	18
AATCGTT	20	7.031042E-4	45.0	22
GATAGCG	20	7.031042E-4	45.0	1
TTACCGG	30	2.1640535E-6	44.999996	2
CGTTTTT	675	0.0	42.666668	1
TACGGGA	95	0.0	40.263157	4
TCTCGTA	45	1.9261279E-8	40.0	28
TTTTGCG	470	0.0	39.734043	1
AGGCACG	125	0.0	39.6	10
ATGGGCG	40	3.4551522E-7	39.375	5
CGCATGG	40	3.4551522E-7	39.375	2
CGGGTAA	75	0.0	39.0	6
>>END_MODULE