SRR2933952.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933952.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469437
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	4775	1.0171758936768938	RNA PCR Primer, Index 11 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	4352	0.9270679558705428	TruSeq Adapter, Index 11 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	3652	0.7779531651744537	Illumina PCR Primer Index 11 (100% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	3096	0.6595134171358457	Illumina PCR Primer Index 11 (100% over 24bp)
TCCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	1398	0.29780353913304664	TruSeq Adapter, Index 11 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	1077	0.22942375654241143	TruSeq Adapter, Index 11 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	971	0.20684351680843224	No Hit
TGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	864	0.18405025594488716	TruSeq Adapter, Index 11 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	749	0.15955282604481538	Illumina PCR Primer Index 11 (100% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	650	0.13846373421779706	Illumina PCR Primer Index 11 (100% over 22bp)
TTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTG	596	0.12696059322124162	TruSeq Adapter, Index 11 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	579	0.1233392340186223	TruSeq Adapter, Index 11 (95% over 23bp)
GGCCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	473	0.10075899428464308	TruSeq Adapter, Index 11 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAA	25	3.8872677E-5	45.0	29
CGTGGCA	25	3.8872677E-5	45.0	15
CGAAAGT	20	7.029017E-4	45.0	38
CGACGAA	25	3.8872677E-5	45.0	19
TTAGCGG	40	6.7993824E-9	45.0	2
TAGACCG	20	7.029017E-4	45.0	39
ACCGCGT	20	7.029017E-4	45.0	23
TTGTCGA	20	7.029017E-4	45.0	44
TCTTACG	40	6.7993824E-9	45.0	1
GAATCGT	50	2.1827873E-11	45.0	33
GCACGGG	35	1.2098826E-7	45.0	3
CGCGCAT	25	3.8872677E-5	45.0	36
CGTCTAG	20	7.029017E-4	45.0	30
CGCGAGT	20	7.029017E-4	45.0	15
GCGCATA	25	3.8872677E-5	45.0	20
AGAACGA	25	3.8872677E-5	45.0	32
CGCCTAC	20	7.029017E-4	45.0	32
TACGCGC	25	3.8872677E-5	45.0	34
GCCGAAA	20	7.029017E-4	45.0	43
CGTAGGG	20	7.029017E-4	45.0	3