SRR2933951.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933951.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	916298
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	3717	0.4056540557766141	TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	3123	0.3408279839091649	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	3005	0.327950077376574	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	2964	0.32347555053050425	TruSeq Adapter, Index 16 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	1205	0.13150743535400056	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1107	0.12081222484388267	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACACG	20	7.032535E-4	45.000004	11
CGTCGAA	20	7.032535E-4	45.000004	25
CTTTACG	20	7.032535E-4	45.000004	1
TATTTCG	25	3.8901835E-5	45.0	1
CAACGTA	25	3.8901835E-5	45.0	35
TCTAGCG	35	1.2115743E-7	45.0	1
TCATCGG	35	1.2115743E-7	45.0	2
TGCTACG	45	3.8380676E-10	45.0	1
TTACCGG	45	3.8380676E-10	45.0	2
CCGGGAC	265	0.0	41.603775	5
TGTTTCG	190	0.0	40.263157	1
TACGGGA	285	0.0	40.263157	4
TTTCGCG	325	0.0	40.153847	1
TTTGGGA	6005	0.0	39.604496	4
TTGGGAC	1935	0.0	39.53488	5
CCGTATC	40	3.4571895E-7	39.375004	38
TTACGGG	320	0.0	39.375004	3
ACGAACT	40	3.4571895E-7	39.375004	13
TTGGGAT	3085	0.0	39.311184	5
TGCGTTG	155	0.0	39.193546	1