##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933950.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1830589
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48517717521519	33.0	31.0	34.0	31.0	34.0
2	32.717366923979114	34.0	31.0	34.0	31.0	34.0
3	32.773096527948105	34.0	31.0	34.0	31.0	34.0
4	36.12418516663216	37.0	35.0	37.0	35.0	37.0
5	36.1591629797841	37.0	35.0	37.0	35.0	37.0
6	35.83056218517647	37.0	35.0	37.0	35.0	37.0
7	36.13844888175336	37.0	35.0	37.0	35.0	37.0
8	36.154258001113305	37.0	37.0	37.0	35.0	37.0
9	38.03095178655613	39.0	39.0	39.0	37.0	39.0
10	37.68390392381905	39.0	37.0	39.0	35.0	39.0
11	37.607550903015365	39.0	37.0	39.0	35.0	39.0
12	37.38778065420474	39.0	37.0	39.0	35.0	39.0
13	37.29167715964643	39.0	37.0	39.0	34.0	39.0
14	38.20914252188776	40.0	38.0	41.0	33.0	41.0
15	38.379526480274926	40.0	38.0	41.0	33.0	41.0
16	38.55191307278695	40.0	38.0	41.0	34.0	41.0
17	38.586454414398865	40.0	38.0	41.0	35.0	41.0
18	38.554950892854706	40.0	38.0	41.0	35.0	41.0
19	38.575417529549235	40.0	38.0	41.0	35.0	41.0
20	38.50536084287626	40.0	38.0	41.0	34.0	41.0
21	38.43603015204396	40.0	38.0	41.0	34.0	41.0
22	38.49452225485896	40.0	38.0	41.0	34.0	41.0
23	38.47233376798397	40.0	37.0	41.0	34.0	41.0
24	38.45295912954792	40.0	37.0	41.0	34.0	41.0
25	38.346546931069724	40.0	37.0	41.0	34.0	41.0
26	38.27794551371171	40.0	37.0	41.0	34.0	41.0
27	38.276534492450246	40.0	37.0	41.0	34.0	41.0
28	38.22181549217219	40.0	37.0	41.0	34.0	41.0
29	38.19956254516989	40.0	37.0	41.0	34.0	41.0
30	38.145494155159895	40.0	37.0	41.0	34.0	41.0
31	38.02817508463123	40.0	37.0	41.0	34.0	41.0
32	37.9602253700858	40.0	37.0	41.0	34.0	41.0
33	37.9222146533165	40.0	37.0	41.0	34.0	41.0
34	37.839188370519	40.0	36.0	41.0	33.0	41.0
35	37.77715806224117	40.0	36.0	41.0	33.0	41.0
36	37.68104200342076	40.0	36.0	41.0	33.0	41.0
37	37.598506819389826	40.0	36.0	41.0	33.0	41.0
38	37.57980955856284	40.0	36.0	41.0	33.0	41.0
39	37.56056001647557	40.0	36.0	41.0	33.0	41.0
40	37.41293102930259	40.0	35.0	41.0	33.0	41.0
41	37.26256139417423	40.0	35.0	41.0	32.0	41.0
42	37.277572956026724	40.0	35.0	41.0	32.0	41.0
43	37.19647774568732	39.0	35.0	41.0	32.0	41.0
44	37.11658433433173	39.0	35.0	41.0	32.0	41.0
45	37.176600536767126	39.0	35.0	41.0	32.0	41.0
46	37.149971402646905	39.0	35.0	41.0	32.0	41.0
47	37.07928541032422	39.0	35.0	41.0	32.0	41.0
48	37.01266641501724	39.0	35.0	41.0	32.0	41.0
49	37.01189999502892	39.0	35.0	41.0	32.0	41.0
50	36.93166680232428	39.0	35.0	41.0	32.0	41.0
51	36.024792020491766	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	19.0
10	32.0
11	25.0
12	30.0
13	21.0
14	41.0
15	51.0
16	97.0
17	161.0
18	260.0
19	477.0
20	764.0
21	1222.0
22	1966.0
23	2854.0
24	4185.0
25	6385.0
26	8817.0
27	11068.0
28	13279.0
29	15972.0
30	20474.0
31	26977.0
32	37386.0
33	58592.0
34	114766.0
35	110483.0
36	115074.0
37	190801.0
38	383891.0
39	703958.0
40	453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.41480747453415	4.4127873596967975	57.97254326339774	15.199861902371314
2	19.117289571826333	3.9812868972773243	55.65421839637407	21.247205134522275
3	20.490126401939484	4.281955152139557	53.76881429965984	21.459104146261122
4	18.78411811717431	4.565852848454787	52.54898833107814	24.101040703292764
5	18.768658612064204	5.388320371202929	51.76394045850816	24.079080558224703
6	20.499030639865094	5.857185856574032	54.87845715231545	18.765326351245417
7	84.7435989181624	1.779099513872311	8.980934551666158	4.496367016299126
8	87.33221930209348	2.1526404889355284	6.396247328045781	4.11889288092521
9	82.2096057607688	4.6884363448048685	8.531188595583169	4.570769298843159
10	37.5297240396397	35.10296412793915	14.874556768340682	12.492755064080468
11	27.203375525582203	25.201069164077794	29.300350870676052	18.295204439663955
12	25.633170525989176	22.267969489601434	32.39749610644443	19.701363877964962
13	25.66916986827737	21.338104839480625	34.072803889895546	18.919921402346457
14	19.606858776055137	23.814193136744514	35.63388614265682	20.945061944543532
15	17.69818348083595	25.15785902788665	36.62329446970347	20.52066302157393
16	23.848280526103892	25.657479641798346	30.20027980065432	20.29396003144343
17	24.79475185309209	24.932630972872666	28.68371873752109	21.58889843651415
18	24.423122830957684	24.17156445275264	31.476098676436926	19.929214039852745
19	23.90940839259932	25.889372218449907	29.935829396986435	20.265389991964337
20	22.352532436281436	30.339032956059498	29.28696720017437	18.021467407484696
21	23.084264135750843	26.144208230247205	33.12278179318241	17.64874584081954
22	21.923107808470387	23.159540453919476	33.80026865670011	21.117083080910025
23	20.350826974268937	24.649498057728962	32.99987053347311	21.999804434528997
24	21.09555995365426	26.693648874761074	30.333788742311903	21.877002429272764
25	22.30959543622299	26.987051708493826	27.74232774260088	22.96102511268231
26	20.1473951826434	27.668089341736458	27.585274466305655	24.599241009314486
27	20.14919788111914	25.907836221019576	32.03378803215796	21.909177865703334
28	18.900911127511417	25.876480192987067	30.033939895847727	25.188668783653785
29	19.954888836325356	27.15442953060463	29.329139419061296	23.561542214008714
30	23.97419628327276	24.920612983034424	30.218798430450526	20.88639230324229
31	22.204438025138355	27.315142831077864	29.561195877392464	20.91922326639131
32	23.966548471557513	27.35736967719133	26.990493223765682	21.68558862748547
33	24.99845678085032	25.101319848420374	27.944666989695666	21.955556381033645
34	20.44702552020142	26.237730042079356	30.811831601741297	22.50341283597793
35	20.326080840647464	29.10429375463307	27.598658136807337	22.970967267912133
36	24.941262074665584	27.55173334921165	27.022941796328944	20.484062779793828
37	23.07809125915211	28.512462382326127	27.515133107431545	20.894313251090225
38	22.734759140364112	26.429580861678946	28.071347528036057	22.764312469920885
39	20.978821570543687	24.77044273728292	29.33356422441083	24.91717146776256
40	22.3278409298865	25.79361069032973	29.98166164005137	21.896886739732402
41	20.401411786042633	27.223860735533755	27.576643364512734	24.79808411391088
42	21.883503069230724	25.79219038243975	29.784839742836866	22.53946680549266
43	22.412349249339965	23.92240967251524	30.167667346411452	23.497573731733336
44	21.987021663519226	23.86434093070591	29.035518076422395	25.113119329352468
45	20.930312593378414	23.21405842600387	28.072877090379105	27.782751890238604
46	21.38874427848086	24.583399113618622	29.942985563662845	24.084871044237673
47	19.454940459054434	26.187309111985268	31.832432075140844	22.525318353819454
48	20.227861087333093	27.063038180607446	29.606864238777792	23.10223649328167
49	23.430054479733027	23.824790818692783	30.320186562904073	22.424968138670124
50	20.90065000936857	24.561111205191335	28.847491162680427	25.69074762275967
51	19.79641525214016	25.278093553495623	27.306730238191097	27.618760956173123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	262.0
1	831.0
2	1400.0
3	3796.0
4	6192.0
5	4223.5
6	2255.0
7	2248.5
8	2242.0
9	2434.0
10	2626.0
11	2631.0
12	2636.0
13	2722.0
14	2808.0
15	2800.0
16	2792.0
17	3033.5
18	3275.0
19	4033.5
20	4792.0
21	6064.5
22	7337.0
23	8455.5
24	9574.0
25	12255.0
26	18098.0
27	21260.0
28	27187.5
29	33115.0
30	36954.0
31	40793.0
32	47731.5
33	54670.0
34	57670.5
35	60671.0
36	65113.5
37	69556.0
38	77139.0
39	84722.0
40	91376.5
41	98031.0
42	102906.0
43	107781.0
44	116879.0
45	125977.0
46	140834.5
47	155692.0
48	178283.0
49	200874.0
50	193492.5
51	186111.0
52	159789.5
53	133468.0
54	117429.5
55	101391.0
56	92399.5
57	83408.0
58	75120.0
59	66832.0
60	55995.0
61	45158.0
62	38557.5
63	31957.0
64	27979.5
65	24002.0
66	20417.0
67	16832.0
68	13509.0
69	10186.0
70	8199.0
71	6212.0
72	5023.0
73	3834.0
74	3117.5
75	1953.5
76	1506.0
77	1037.0
78	568.0
79	447.0
80	326.0
81	203.0
82	80.0
83	51.5
84	23.0
85	19.5
86	16.0
87	9.0
88	2.0
89	4.0
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1830589.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.251721863906447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.39559327211452	19.605449997045568
2	12.373175204502061	6.991270089019521
3	4.789289449562265	4.059170203643241
4	2.360084424917587	2.6670579499243705
5	1.4502309038179688	2.048576006655346
6	0.9374930957567008	1.5891476514390552
7	0.6692132438830228	1.3234498503678027
8	0.5276702551709614	1.192607462795724
9	0.4461029443447028	1.1342858675666654
>10	6.591589408470225	45.815710048947835
>50	0.37947855691964955	6.4907793500615405
>100	0.07304791062644138	3.8707569278315264
>500	0.0041016091164767	0.7667781034615
>1k	0.0021484619181544617	1.136372212340683
>5k	7.812588793288951E-4	1.3085882788996452
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	6711	0.36660331729295875	No Hit
CTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGCT	6330	0.3457903439821828	No Hit
TCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	5638	0.3079883032182538	No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	5036	0.2751027128426971	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3602	0.19676726998796562	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2426	0.13252565157990132	No Hit
TCCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTG	2274	0.12422231314620595	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTG	2092	0.11428015791638647	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1926	0.10521203831116652	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.4627226537469634E-5	0.0	0.0	0.3626155297557234	0.0
2	5.4627226537469634E-5	0.0	0.0	1.3858927372556047	0.0
3	5.4627226537469634E-5	0.0	0.0	2.025249796650149	0.0
4	5.4627226537469634E-5	0.0	0.0	2.6879873090027306	0.0
5	5.4627226537469634E-5	0.0	0.0	3.816039537001479	0.0
6	5.4627226537469634E-5	0.0	0.0	4.585464022781738	0.0
7	5.4627226537469634E-5	0.0	0.0	5.281087125509877	0.0
8	5.4627226537469634E-5	0.0	0.0	6.325286560773609	0.0
9	5.4627226537469634E-5	0.0	0.0	6.848069118737193	0.0
10	5.4627226537469634E-5	0.0	0.0	7.813659975013507	0.0
11	5.4627226537469634E-5	0.0	0.0	9.686390555171041	0.0
12	5.4627226537469634E-5	0.0	0.0	11.32564436910743	0.0
13	5.4627226537469634E-5	0.0	0.0	11.83619042832662	0.0
14	1.0925445307493927E-4	0.0	0.0	12.054262316664198	0.0
15	1.0925445307493927E-4	0.0	0.0	12.333571325950281	0.0
16	1.0925445307493927E-4	0.0	0.0	12.945396263169942	0.0
17	1.0925445307493927E-4	0.0	0.0	13.854065549394212	0.0
18	1.0925445307493927E-4	0.0	0.0	14.79234279240179	0.0
19	1.0925445307493927E-4	0.0	0.0	15.513749946055613	0.0
20	1.0925445307493927E-4	0.0	0.0	16.301037534913625	0.0
21	1.0925445307493927E-4	0.0	0.0	17.388501733595035	0.0
22	1.0925445307493927E-4	0.0	0.0	18.58090483445492	0.0
23	1.638816796124089E-4	0.0	0.0	19.684811828324108	0.0
24	1.638816796124089E-4	0.0	0.0	20.467674611832585	0.0
25	1.638816796124089E-4	0.0	0.0	21.184493078457262	0.0
26	1.638816796124089E-4	0.0	0.0	21.83930964296191	0.0
27	1.638816796124089E-4	0.0	0.0	22.59917436409811	0.0
28	1.638816796124089E-4	0.0	0.0	23.28381739429222	0.0
29	1.638816796124089E-4	0.0	0.0	24.08388775416	0.0
30	1.638816796124089E-4	0.0	0.0	24.989661797377785	0.0
31	1.638816796124089E-4	0.0	0.0	25.723742467588302	0.0
32	1.638816796124089E-4	0.0	0.0	26.405654136455535	0.0
33	1.638816796124089E-4	0.0	0.0	27.10045783078561	0.0
34	1.638816796124089E-4	0.0	0.0	27.853821911963855	0.0
35	1.638816796124089E-4	0.0	0.0	28.632533026255484	0.0
36	1.638816796124089E-4	0.0	0.0	29.312041097155067	0.0
37	2.1850890614987854E-4	0.0	0.0	30.050054927676282	0.0
38	2.731361326873482E-4	0.0	0.0	30.930755073913367	0.0
39	2.731361326873482E-4	0.0	0.0	32.533736409428876	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	20	7.0343824E-4	45.000004	24
GTACGGA	20	7.0343824E-4	45.000004	9
TCGTACG	80	0.0	42.187504	30
GTTTACG	70	0.0	41.785713	1
CGGTCGT	55	6.184564E-11	40.909092	6
CTCCGCG	50	1.0822987E-9	40.5	1
ACACGCG	305	0.0	39.836067	36
CGTTTTT	2260	0.0	39.723454	1
CTACGAA	125	0.0	39.600002	11
TACGAAT	120	0.0	39.375004	12
CCCGATC	40	3.4597178E-7	39.375004	41
CCGATAA	70	0.0	38.571426	15
AAGCGTA	70	0.0	38.571426	11
CGCGTAA	35	6.2503204E-6	38.571426	13
TGGGCGA	1820	0.0	38.324173	6
TTTGGGA	13885	0.0	38.27512	4
TTGTTCG	485	0.0	38.041237	1
TATGCGG	160	0.0	37.968754	2
TTGGGAC	4660	0.0	37.70923	5
GTATTCG	30	1.1401861E-4	37.500004	1
>>END_MODULE