##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933949.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1147974
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39282509882628	33.0	31.0	34.0	31.0	34.0
2	32.607463235230064	34.0	31.0	34.0	31.0	34.0
3	32.676009212752206	34.0	31.0	34.0	31.0	34.0
4	36.060454330847215	37.0	35.0	37.0	35.0	37.0
5	36.0887668187607	37.0	35.0	37.0	35.0	37.0
6	35.619658633383686	37.0	35.0	37.0	33.0	37.0
7	36.001342364896765	37.0	35.0	37.0	35.0	37.0
8	35.97821988999751	37.0	35.0	37.0	35.0	37.0
9	37.86385144611289	39.0	38.0	39.0	35.0	39.0
10	37.58060112859699	39.0	37.0	39.0	35.0	39.0
11	37.537110596581456	39.0	37.0	39.0	35.0	39.0
12	37.3133877596531	39.0	37.0	39.0	35.0	39.0
13	37.221696658635125	39.0	37.0	39.0	34.0	39.0
14	38.35785653682052	40.0	38.0	41.0	34.0	41.0
15	38.380633185072135	40.0	38.0	41.0	34.0	41.0
16	38.51142883026967	40.0	38.0	41.0	34.0	41.0
17	38.46291379421485	40.0	38.0	41.0	34.0	41.0
18	38.44585591659742	40.0	38.0	41.0	34.0	41.0
19	38.47303684578222	40.0	38.0	41.0	34.0	41.0
20	38.38445121579409	40.0	37.0	41.0	34.0	41.0
21	38.344264765578316	40.0	37.0	41.0	34.0	41.0
22	38.36914773331103	40.0	37.0	41.0	34.0	41.0
23	38.311820650990356	40.0	37.0	41.0	34.0	41.0
24	38.26543719631281	40.0	37.0	41.0	34.0	41.0
25	38.19079090641426	40.0	37.0	41.0	34.0	41.0
26	38.1443586701441	40.0	37.0	41.0	34.0	41.0
27	38.081614217743606	40.0	37.0	41.0	34.0	41.0
28	38.02071039936445	40.0	37.0	41.0	34.0	41.0
29	37.98304404106713	40.0	37.0	41.0	34.0	41.0
30	37.92397824340969	40.0	36.0	41.0	34.0	41.0
31	37.88600787125841	40.0	36.0	41.0	33.0	41.0
32	37.74206384465153	40.0	36.0	41.0	33.0	41.0
33	37.6779082104647	40.0	36.0	41.0	33.0	41.0
34	37.585807692508716	40.0	36.0	41.0	33.0	41.0
35	37.50497136694734	40.0	36.0	41.0	33.0	41.0
36	37.445439530860455	40.0	36.0	41.0	33.0	41.0
37	37.32414410082458	40.0	35.0	41.0	32.0	41.0
38	37.31176577169866	39.0	35.0	41.0	32.0	41.0
39	37.27490692297909	39.0	35.0	41.0	32.0	41.0
40	37.13945524898647	39.0	35.0	41.0	32.0	41.0
41	37.03039267439855	39.0	35.0	41.0	32.0	41.0
42	36.9697179552847	39.0	35.0	41.0	32.0	41.0
43	36.86472951477995	39.0	35.0	41.0	32.0	41.0
44	36.743000277009756	39.0	35.0	41.0	31.0	41.0
45	36.79731248268689	39.0	35.0	41.0	32.0	41.0
46	36.72615320556041	39.0	35.0	41.0	31.0	41.0
47	36.59979494309105	39.0	35.0	41.0	31.0	41.0
48	36.55486274079378	39.0	35.0	40.0	31.0	41.0
49	36.540910334206174	39.0	35.0	40.0	31.0	41.0
50	36.43899861843561	38.0	35.0	40.0	31.0	41.0
51	35.437998595787015	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	10.0
10	28.0
11	18.0
12	12.0
13	12.0
14	12.0
15	30.0
16	49.0
17	100.0
18	178.0
19	353.0
20	665.0
21	1164.0
22	1700.0
23	2393.0
24	3212.0
25	4377.0
26	6155.0
27	7520.0
28	9103.0
29	10955.0
30	13999.0
31	18370.0
32	25170.0
33	36637.0
34	68622.0
35	85168.0
36	83142.0
37	132904.0
38	251999.0
39	383692.0
40	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.507133436819995	4.494091329594572	52.69492166198886	16.30385357159657
2	19.3878084346858	7.709843602729678	51.49567847355427	21.40666948903024
3	20.818851298025915	7.845038302261201	49.353818117831935	21.982292281880948
4	19.36794735769277	4.777373006705727	52.03349553212878	23.821184103472724
5	21.07469332929143	5.645511135269614	47.729739523717434	25.550056011721523
6	21.45327333197442	9.018671154573186	50.848189941584046	18.679865571868355
7	81.28468066349934	2.5709641507560277	11.381877986783673	4.762477198960952
8	81.96588076036565	5.297245407997045	7.565589464569755	5.171284367067547
9	76.2136598912519	5.733143782002031	9.664069046859947	8.38912727988613
10	40.23584157829359	28.541848508764133	16.86675830637279	14.355551606569486
11	32.24803000764826	23.006705726784755	24.621724882270854	20.123539383296137
12	27.451754133804425	21.547090787770454	29.565042413852577	21.436112664572544
13	25.532372684398773	22.48779153534836	31.58773630761672	20.39209947263614
14	22.08412385646365	23.775102920449417	31.064815056786998	23.075958166299934
15	18.782394026345546	24.3475026437881	35.261948441341005	21.60815488852535
16	22.212088427089814	24.847601078073197	28.20778170934185	24.73252878549514
17	21.869920398894052	24.383740398301704	28.944035317873052	24.80230388493119
18	21.967048034188927	23.293907353302426	31.31804378844817	23.421000824060474
19	22.516624940982986	25.876718462264826	27.500448616432077	24.106207980320114
20	24.163700571615735	27.709599694766606	29.175051002897277	18.951648730720382
21	25.058494356143957	27.4598553625779	28.117448653018272	19.364201628259874
22	22.5574795247976	22.766891933092563	29.095780914898768	25.579847627211073
23	22.43709352302404	24.06561472646593	29.320089130938502	24.17720261957152
24	23.140855106474536	23.230142842956372	28.847953002419914	24.78104904814917
25	21.14551374856922	27.40610850071517	25.714171226874477	25.73420652384113
26	21.75441255638194	24.884187272534046	26.63152649798689	26.729873673097128
27	20.981050093469015	26.75051873997146	27.08023004005317	25.18820112650635
28	18.58735476587449	27.04852200485377	28.424424246542166	25.939698982729574
29	21.737774548900934	25.885690790906413	27.15819347824951	25.218341181943142
30	22.554953335180063	25.529498054833994	28.686015536937248	23.229533073048692
31	25.535682863897613	25.300137459559192	25.279927942618908	23.884251733924287
32	25.6155627218038	28.634097984797563	24.166662311167325	21.583676982231307
33	24.19619259669644	26.549817330357655	25.45127328667722	23.80271678626868
34	24.161174381998197	25.457632315714466	27.189553073501667	23.19164022878567
35	23.11733541003542	27.58599062348102	25.48097779217996	23.8156961743036
36	25.13027298527667	26.503649037347536	25.007622123846012	23.358455853529783
37	21.87828295762796	28.2783407986592	27.385289213867214	22.458087029845625
38	21.561028385660304	27.18371670438529	25.953288140672175	25.30196676928223
39	24.604912654816225	25.712429027138246	26.931794622526294	22.750863695519236
40	24.312223099129422	24.91685351758838	28.463188190673307	22.30773519260889
41	24.298372611226387	25.941092742518556	25.30126988938774	24.459264756867316
42	22.144839517271297	25.805375383066163	27.266558301843073	24.783226797819463
43	23.912126929703984	23.335981476932403	27.429628197154294	25.322263396209323
44	22.03464538395469	22.983708690266504	27.151573119251832	27.830072806526978
45	21.894746745135343	22.003808448623403	25.93098798404842	30.170456822192836
46	23.455235048877412	24.682179213118065	27.360201537665485	24.50238420033903
47	19.94435414042478	24.287832302822192	32.15186058220831	23.61595297454472
48	20.615275258847326	24.43069268119313	30.298856942753062	24.655175117206486
49	22.662882608839574	22.59511103910019	31.63259795082467	23.10940840123557
50	23.227529543352027	22.859315629099612	27.926242231966924	25.986912595581437
51	21.168859225034716	23.267774357258965	26.322111824832266	29.241254592874057
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	210.0
1	591.0
2	972.0
3	2006.5
4	3041.0
5	2153.5
6	1266.0
7	1330.0
8	1394.0
9	1486.5
10	1579.0
11	1548.5
12	1518.0
13	1589.5
14	1661.0
15	1521.5
16	1382.0
17	1471.0
18	1560.0
19	1632.5
20	1705.0
21	2021.0
22	2337.0
23	2980.5
24	3624.0
25	4294.0
26	6405.0
27	7846.0
28	9147.0
29	10448.0
30	12715.5
31	14983.0
32	17553.0
33	20123.0
34	23552.5
35	26982.0
36	28461.0
37	29940.0
38	34450.5
39	38961.0
40	43090.0
41	47219.0
42	52706.5
43	58194.0
44	65346.0
45	72498.0
46	97351.0
47	122204.0
48	126068.0
49	129932.0
50	129086.5
51	128241.0
52	110391.0
53	92541.0
54	82162.0
55	71783.0
56	64994.0
57	58205.0
58	54706.0
59	51207.0
60	46865.0
61	42523.0
62	38081.0
63	33639.0
64	28124.5
65	22610.0
66	18704.5
67	14799.0
68	12834.0
69	10869.0
70	8567.0
71	6265.0
72	5073.5
73	3882.0
74	3225.5
75	1945.0
76	1321.0
77	929.5
78	538.0
79	380.0
80	222.0
81	191.5
82	161.0
83	93.0
84	25.0
85	24.5
86	24.0
87	13.5
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1147974.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.34928796521579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.25591563539709	19.633558956425627
2	12.523662404645963	7.1007382377691055
3	4.901560595424435	4.168672583959256
4	2.47134412304266	2.802433848211202
5	1.4080326080314451	1.9958360934748627
6	0.9297496684790673	1.5814644652366192
7	0.6788614185234879	1.3471666489538063
8	0.5108077785597975	1.1584829447411105
9	0.41069818481844383	1.0478700997388533
>10	6.559857742147798	45.53249121944415
>50	0.27383996850751685	4.850524575039964
>100	0.06729656686768466	3.468345380771037
>500	0.003411346707461091	0.5987181759843868
>1k	0.003411346707461091	1.7688121854082057
>5k	0.0015506121397550416	2.9448845848418537
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	8064	0.7024549336483231	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	7043	0.6135156371137325	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	6664	0.5805009521121558	No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	6165	0.5370330686931933	No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	5560	0.4843315266722069	No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT	3930	0.34234224816938363	No Hit
TCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	3751	0.32674956053011656	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT	1614	0.14059551871383846	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT	1532	0.13345249979529153	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC	1515	0.13197163001949522	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1501	0.13075209020413356	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT	1310	0.11411408272312788	No Hit
TCCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG	1295	0.11280743292095466	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA	1260	0.10975858338255048	No Hit
CGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG	1231	0.10723239376501559	No Hit
GCCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTG	1180	0.10278978443762664	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.7109986811548E-5	0.0	0.0	0.3577607158350276	0.0
2	8.7109986811548E-5	0.0	0.0	1.8016087472364357	0.0
3	8.7109986811548E-5	0.0	0.0	2.4884709932454916	0.0
4	8.7109986811548E-5	0.0	0.0	3.3133154583640394	0.0
5	8.7109986811548E-5	0.0	0.0	5.080515760809914	0.0
6	8.7109986811548E-5	0.0	0.0	5.829226097455169	0.0
7	8.7109986811548E-5	0.0	0.0	6.629592656279672	0.0
8	8.7109986811548E-5	0.0	0.0	7.717596391555906	0.0
9	8.7109986811548E-5	0.0	0.0	8.163599524031032	0.0
10	8.7109986811548E-5	0.0	0.0	9.732798826454257	0.0
11	8.7109986811548E-5	0.0	0.0	11.491201020232166	0.0
12	8.7109986811548E-5	0.0	0.0	13.830278386095852	0.0
13	8.7109986811548E-5	0.0	0.0	14.391963581056714	0.0
14	8.7109986811548E-5	0.0	0.0	14.629338295118183	0.0
15	8.7109986811548E-5	0.0	0.0	15.053041270969551	0.0
16	8.7109986811548E-5	0.0	0.0	15.778667461109746	0.0
17	8.7109986811548E-5	0.0	0.0	16.704820840890125	0.0
18	8.7109986811548E-5	0.0	0.0	17.663640465724832	0.0
19	1.74219973623096E-4	0.0	0.0	18.542493122666542	0.0
20	1.74219973623096E-4	0.0	0.0	19.374045056769578	0.0
21	1.74219973623096E-4	0.0	0.0	20.41849379864004	0.0
22	1.74219973623096E-4	0.0	0.0	21.584635192086232	0.0
23	1.74219973623096E-4	0.0	0.0	22.66497324852305	0.0
24	1.74219973623096E-4	0.0	0.0	23.490079043602034	0.0
25	1.74219973623096E-4	0.0	0.0	24.22206426277947	0.0
26	1.74219973623096E-4	0.0	0.0	24.861103126028986	0.0
27	1.74219973623096E-4	0.0	0.0	25.54439386257877	0.0
28	1.74219973623096E-4	0.0	0.0	26.21627319085624	0.0
29	1.74219973623096E-4	0.0	0.0	26.914459735150796	0.0
30	1.74219973623096E-4	0.0	0.0	27.73869443036166	0.0
31	1.74219973623096E-4	0.0	0.0	28.43391923510463	0.0
32	1.74219973623096E-4	0.0	0.0	29.08280152686385	0.0
33	1.74219973623096E-4	0.0	0.0	29.770099322806963	0.0
34	1.74219973623096E-4	0.0	0.0	30.44467906067559	0.0
35	1.74219973623096E-4	0.0	0.0	31.15026995384913	0.0
36	1.74219973623096E-4	0.0	0.0	31.8198844224695	0.0
37	1.74219973623096E-4	0.0	0.0	32.4784359227648	0.0
38	1.74219973623096E-4	0.0	0.0	33.21181490173122	0.0
39	1.74219973623096E-4	0.0	0.0	34.13143503250074	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGAAC	20	7.033281E-4	45.000004	26
CGTCGAC	20	7.033281E-4	45.000004	31
CCGCGAA	20	7.033281E-4	45.000004	35
ACGCCCG	45	3.8562575E-10	45.0	28
CTACGGC	75	0.0	42.0	6
TACGGGA	210	0.0	40.714287	4
TATTGCG	50	1.0822987E-9	40.5	1
TACGGCT	1090	0.0	40.458717	7
TTACACG	180	0.0	40.0	34
CCCGCAT	45	1.9283107E-8	40.0	23
ACACGCG	170	0.0	39.705883	36
CGTTTTT	1100	0.0	39.272728	1
GTTTGCG	235	0.0	39.255318	1
TTGTGCG	345	0.0	38.47826	1
TTTGGGA	5845	0.0	38.41745	4
TTGGGAC	2135	0.0	37.728336	5
TCTCGCA	30	1.1399209E-4	37.500004	11
TTCTACG	90	0.0	37.5	1
ATACGGC	2210	0.0	37.46606	6
TGGGCGA	1265	0.0	37.35178	6
>>END_MODULE