SRR2933948.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933948.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1545342
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	7834	0.5069427997168264	Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	7234	0.4681164428327192	Illumina PCR Primer Index 11 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	6610	0.4277370316732477	Illumina PCR Primer Index 11 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	6505	0.420942419218529	Illumina PCR Primer Index 11 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	2563	0.16585325448994462	Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1943	0.1257326857097005	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	1942	0.12566797511489367	Illumina PCR Primer Index 11 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	1754	0.11350238329120674	Illumina PCR Primer Index 11 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	1624	0.10509000596631685	Illumina PCR Primer Index 11 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCG	30	2.165989E-6	45.000004	32
TTATCCG	25	3.8914317E-5	45.0	1
CGTAGAC	20	7.0340425E-4	45.0	14
CGAATCG	20	7.0340425E-4	45.0	40
TAGTCGC	25	3.8914317E-5	45.0	41
CGTTTGG	960	0.0	41.250004	2
GGTATGC	230	0.0	41.086956	8
CGTCATA	55	6.184564E-11	40.909092	38
GATCGAC	50	1.0822987E-9	40.5	9
CTTTACG	45	1.9290383E-8	40.0	1
TCGCGCG	45	1.9290383E-8	40.0	1
TTTGTCG	360	0.0	39.375	1
CCTCGCG	40	3.459245E-7	39.375	1
TTTGGGA	12640	0.0	39.285995	4
TTGGGAC	4670	0.0	38.881157	5
TTTCGCG	545	0.0	38.807343	1
TTGGGAT	8145	0.0	38.75691	5
TACGCGC	70	0.0	38.571426	34
TACGCGG	65	9.094947E-12	38.07692	2
CGCTTGG	545	0.0	37.98165	2