##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933948.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1545342
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4317575009286	33.0	31.0	34.0	31.0	34.0
2	32.654877690504755	34.0	31.0	34.0	31.0	34.0
3	32.71701798048587	34.0	31.0	34.0	31.0	34.0
4	36.08977947923502	37.0	35.0	37.0	35.0	37.0
5	36.13292656253438	37.0	35.0	37.0	35.0	37.0
6	35.79843232113021	37.0	35.0	37.0	35.0	37.0
7	36.11045645559365	37.0	35.0	37.0	35.0	37.0
8	36.120293113110236	37.0	37.0	37.0	35.0	37.0
9	37.95960311697993	39.0	39.0	39.0	35.0	39.0
10	37.58339513195138	39.0	37.0	39.0	35.0	39.0
11	37.52281824994079	39.0	37.0	39.0	35.0	39.0
12	37.47169688004338	39.0	37.0	39.0	35.0	39.0
13	37.48327231124243	39.0	37.0	39.0	35.0	39.0
14	38.761031538649696	40.0	38.0	41.0	35.0	41.0
15	38.80714948535664	40.0	38.0	41.0	35.0	41.0
16	38.83891526924137	40.0	38.0	41.0	35.0	41.0
17	38.792207809015736	40.0	38.0	41.0	35.0	41.0
18	38.74711681944838	40.0	38.0	41.0	35.0	41.0
19	38.78403486089164	40.0	38.0	41.0	35.0	41.0
20	38.74598114850952	40.0	38.0	41.0	35.0	41.0
21	38.6855006852852	40.0	38.0	41.0	35.0	41.0
22	38.65713673736946	40.0	38.0	41.0	35.0	41.0
23	38.59652944137932	40.0	38.0	41.0	35.0	41.0
24	38.58045468252335	40.0	38.0	41.0	35.0	41.0
25	38.49377678209743	40.0	38.0	41.0	34.0	41.0
26	38.460415882050704	40.0	38.0	41.0	34.0	41.0
27	38.44541208353879	40.0	38.0	41.0	34.0	41.0
28	38.36230167820457	40.0	38.0	41.0	34.0	41.0
29	38.3445683868037	40.0	38.0	41.0	34.0	41.0
30	38.28837888312102	40.0	38.0	41.0	34.0	41.0
31	38.22866912308085	40.0	38.0	41.0	34.0	41.0
32	38.15750299933607	40.0	37.0	41.0	34.0	41.0
33	38.12245638829463	40.0	37.0	41.0	34.0	41.0
34	38.11402265647345	40.0	37.0	41.0	34.0	41.0
35	38.09630748403913	40.0	37.0	41.0	34.0	41.0
36	38.024043868606434	40.0	37.0	41.0	34.0	41.0
37	37.93310089287679	40.0	37.0	41.0	33.0	41.0
38	37.89412376030678	40.0	37.0	41.0	33.0	41.0
39	37.859269986837866	40.0	37.0	41.0	33.0	41.0
40	37.78115070968109	40.0	37.0	41.0	33.0	41.0
41	37.698745649830265	40.0	37.0	41.0	33.0	41.0
42	37.697886940237176	40.0	37.0	41.0	33.0	41.0
43	37.59224754132095	40.0	36.0	41.0	33.0	41.0
44	37.47899623513759	40.0	36.0	41.0	33.0	41.0
45	37.501656591227054	40.0	36.0	41.0	33.0	41.0
46	37.4258785433904	40.0	36.0	41.0	33.0	41.0
47	37.348529322311826	39.0	36.0	41.0	33.0	41.0
48	37.31028471367503	39.0	36.0	41.0	33.0	41.0
49	37.31175558549499	39.0	36.0	41.0	33.0	41.0
50	37.25239785109057	39.0	35.0	41.0	33.0	41.0
51	36.373186000251074	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	24.0
10	25.0
11	24.0
12	17.0
13	12.0
14	18.0
15	26.0
16	40.0
17	77.0
18	132.0
19	246.0
20	480.0
21	839.0
22	1259.0
23	2033.0
24	2942.0
25	4436.0
26	6378.0
27	8026.0
28	10159.0
29	12172.0
30	15664.0
31	20296.0
32	27002.0
33	38626.0
34	67888.0
35	94314.0
36	104588.0
37	170807.0
38	349572.0
39	606830.0
40	388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.09534070775272	4.483926535355928	56.15915441371554	16.261578343175813
2	18.363766726070992	4.0488124958747	55.16345249142262	22.423968286631695
3	19.890872052917736	4.128082974513085	53.692062986704556	22.288981985864616
4	19.061929333442045	4.473443418997219	51.77921780421422	24.685409443346522
5	18.459926669953965	5.682302040583897	50.66393070271823	25.193840586743903
6	21.86105082240695	5.925937430031669	53.311370557455895	18.901641190105494
7	84.73632373934055	1.956524833984969	8.557264346662421	4.749887080012062
8	85.97837889606313	2.390409372164867	6.610122548924445	5.021089182847551
9	80.06182450227845	5.496323791109023	8.968888440228765	5.472963266383752
10	33.277035115851376	39.793844987064354	14.88524870222902	12.04387119485525
11	24.082889095099986	24.689227368440125	31.60556045199056	19.62232308446933
12	23.022735420379437	22.801360475545216	33.64264997651005	20.53325412756529
13	22.447975917305037	22.975561396765247	34.04074955576177	20.53571313016795
14	18.70964485531358	25.78147749818487	32.227041004515506	23.281836641986047
15	16.868757854248447	27.09050812053254	34.341524400423985	21.699209624795028
16	20.292401293694212	26.425995022461045	31.32995802870821	21.951645655136534
17	19.997515113159416	26.56085190203851	30.044029088706576	23.397603896095493
18	20.037117997181205	26.16385240289852	31.79348001930964	22.005549580610634
19	21.1790011531428	26.14282145958629	30.39268977352586	22.285487613745047
20	23.518159734220646	26.92077223035419	30.534664818532082	19.02640321689309
21	24.726694802833286	26.041743510498	29.83003115168034	19.40153053498837
22	22.356475136248157	24.786099128865974	29.91726103347997	22.9401647014059
23	20.92054703748426	26.02388338633131	29.52750912095834	23.528060455226093
24	21.48773540096626	24.44999229943922	30.479401970567032	23.582870329027493
25	19.999327009814007	26.693767463771774	28.285518674830556	25.02138685158366
26	19.59106786717762	27.582955747012633	28.614377917638944	24.2115984681708
27	19.759897809028683	26.868033095586608	30.692429248671164	22.679639846713542
28	17.40080836475033	28.2339443307695	31.121460492240555	23.24378681223962
29	18.237386934413223	26.01217076867127	30.86734198643407	24.883100310481435
30	19.520209765864127	26.2090851086685	30.697994359824555	23.572710765642814
31	19.591779683720496	29.485123681359855	27.467059071713575	23.456037563206074
32	19.167795866546047	28.769100949822114	27.163436960879856	24.899666222751986
33	19.381211408219023	26.995512967356095	27.527951741426815	26.095323882998066
34	18.349595105808294	27.026121078699735	28.443606657943675	26.1806771575483
35	17.673822364240408	25.676258071028933	28.170527947858794	28.47939161687186
36	18.40524621734218	26.19006019379529	29.02101929540516	26.38367429345737
37	18.533179063275316	29.252424382434437	29.661071788639664	22.553324765650583
38	17.93078813621839	29.251259591727912	27.86535278274971	24.952599489303985
39	19.329960617132	26.983929770885666	27.939122860829514	25.746986751152818
40	19.96179486482604	25.92746460006911	28.815757288677844	25.294983246427005
41	17.610729534303733	26.314433957014042	28.008169065488413	28.066667443193804
42	18.93626135832715	25.063125185234075	27.997685949129707	28.00292750730906
43	20.420916534980606	24.551587933285965	28.427881983405616	26.599613548327817
44	19.17368453067347	25.58352778867073	28.83646467901604	26.406323001639763
45	19.517815473856274	24.44235644925201	27.46078214401731	28.579045932874408
46	19.39104741862966	25.43119904849541	29.049880220689012	26.127873312185912
47	16.513496688758863	26.35410155163064	31.043548936093114	26.088852823517385
48	16.8664282728354	25.380660073951265	30.004426204684787	27.748485448528548
49	18.58268266830255	24.253272091226407	30.99663375485815	26.167411485612895
50	18.725175398067222	24.320182846256685	28.628225984927607	28.326415770748483
51	17.30866047774538	23.575752163598736	27.152695002141918	31.96289235651396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	255.0
1	814.5
2	1374.0
3	2609.5
4	3845.0
5	2753.0
6	1661.0
7	1731.0
8	1801.0
9	1921.5
10	2042.0
11	2057.5
12	2073.0
13	2118.5
14	2164.0
15	2336.0
16	2508.0
17	2606.0
18	2704.0
19	3792.0
20	4880.0
21	5379.5
22	5879.0
23	6688.5
24	7498.0
25	9448.5
26	14855.5
27	18312.0
28	21671.5
29	25031.0
30	29371.5
31	33712.0
32	37692.0
33	41672.0
34	46433.5
35	51195.0
36	56370.0
37	61545.0
38	66340.0
39	71135.0
40	77189.5
41	83244.0
42	90500.0
43	97756.0
44	105814.5
45	113873.0
46	129543.5
47	145214.0
48	163760.5
49	182307.0
50	171824.5
51	161342.0
52	134146.0
53	106950.0
54	90409.0
55	73868.0
56	65838.0
57	57808.0
58	53413.0
59	49018.0
60	45873.0
61	42728.0
62	35877.0
63	29026.0
64	24078.0
65	19130.0
66	15318.0
67	11506.0
68	9473.0
69	7440.0
70	5922.0
71	4404.0
72	3965.5
73	3527.0
74	2702.0
75	1298.0
76	719.0
77	593.5
78	468.0
79	355.0
80	242.0
81	171.5
82	101.0
83	70.5
84	40.0
85	43.5
86	47.0
87	32.5
88	18.0
89	10.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1545342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.905061725110986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.66983491932574	17.937560237074187
2	13.613425156999897	7.32540088278522
3	5.694572954719279	4.596385105346096
4	2.917729458255592	3.1400676468616537
5	1.6724516884621239	2.2498707955169763
6	1.0928955862518943	1.7642653924325142
7	0.7602679853786306	1.4318539951966485
8	0.533742702031523	1.1488304274788517
9	0.4242308493843079	1.0272561469542958
>10	5.855438216554577	41.04123543683334
>50	0.677680595313253	10.923851285108768
>100	0.08041906371878972	3.8920666159813995
>500	0.004630188282799379	0.8676355852523314
>1k	0.00170585884103135	0.8058723629060988
>5k	9.74776480589343E-4	1.847848084271628
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	7834	0.5069427997168264	Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	7234	0.4681164428327192	Illumina PCR Primer Index 11 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	6610	0.4277370316732477	Illumina PCR Primer Index 11 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	6505	0.420942419218529	Illumina PCR Primer Index 11 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	2563	0.16585325448994462	Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1943	0.1257326857097005	No Hit
CGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	1942	0.12566797511489367	Illumina PCR Primer Index 11 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	1754	0.11350238329120674	Illumina PCR Primer Index 11 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG	1624	0.10509000596631685	Illumina PCR Primer Index 11 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.235529740342267E-4	0.0	0.0	0.491218125178763	0.0
2	3.235529740342267E-4	0.0	0.0	1.9539364101926953	0.0
3	3.235529740342267E-4	0.0	0.0	2.8024217293000513	0.0
4	3.235529740342267E-4	0.0	0.0	3.642818224056552	0.0
5	3.235529740342267E-4	0.0	0.0	4.97126202484628	0.0
6	3.235529740342267E-4	0.0	0.0	5.727340614569461	0.0
7	3.235529740342267E-4	0.0	0.0	6.4349509687823145	0.0
8	3.235529740342267E-4	0.0	0.0	7.561497713774686	0.0
9	3.235529740342267E-4	0.0	0.0	8.071999596205888	0.0
10	3.235529740342267E-4	0.0	0.0	9.087502960509713	0.0
11	3.235529740342267E-4	0.0	0.0	11.074830037622739	0.0
12	3.235529740342267E-4	0.0	0.0	13.032778504693459	0.0
13	3.235529740342267E-4	0.0	0.0	13.61679162282524	0.0
14	3.235529740342267E-4	0.0	0.0	13.817070913752426	0.0
15	3.235529740342267E-4	0.0	0.0	14.125352187412236	0.0
16	3.235529740342267E-4	0.0	0.0	14.846422345344914	0.0
17	3.235529740342267E-4	0.0	0.0	15.986493604651915	0.0
18	3.882635688410721E-4	0.0	0.0	17.085538346851376	0.0
19	3.882635688410721E-4	0.0	0.0	17.934800193096414	0.0
20	3.882635688410721E-4	0.0	0.0	18.885528251998586	0.0
21	3.882635688410721E-4	0.0	0.0	20.129654147754994	0.0
22	3.882635688410721E-4	0.0	0.0	21.53756255896753	0.0
23	3.882635688410721E-4	0.0	0.0	22.734190878135713	0.0
24	3.882635688410721E-4	0.0	0.0	23.65508735283193	0.0
25	3.882635688410721E-4	0.0	0.0	24.46183433828887	0.0
26	3.882635688410721E-4	0.0	0.0	25.1767569897149	0.0
27	3.882635688410721E-4	0.0	0.0	25.894332775528007	0.0
28	3.882635688410721E-4	0.0	0.0	26.656558871757838	0.0
29	3.882635688410721E-4	0.0	0.0	27.428944531372345	0.0
30	3.882635688410721E-4	0.0	0.0	28.331139644169383	0.0
31	3.882635688410721E-4	0.0	0.0	29.153740725353998	0.0
32	3.882635688410721E-4	0.0	0.0	29.918943508944945	0.0
33	4.529741636479174E-4	0.0	0.0	30.639819535093203	0.0
34	4.529741636479174E-4	0.0	0.0	31.367943147859826	0.0
35	4.529741636479174E-4	0.0	0.0	32.13728740951841	0.0
36	4.529741636479174E-4	0.0	0.0	32.8896774953376	0.0
37	4.529741636479174E-4	0.0	0.0	33.636696601787826	0.0
38	4.529741636479174E-4	0.0	0.0	34.359190392806255	0.0
39	4.529741636479174E-4	0.0	0.0	35.072236436982884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCG	30	2.165989E-6	45.000004	32
TTATCCG	25	3.8914317E-5	45.0	1
CGTAGAC	20	7.0340425E-4	45.0	14
CGAATCG	20	7.0340425E-4	45.0	40
TAGTCGC	25	3.8914317E-5	45.0	41
CGTTTGG	960	0.0	41.250004	2
GGTATGC	230	0.0	41.086956	8
CGTCATA	55	6.184564E-11	40.909092	38
GATCGAC	50	1.0822987E-9	40.5	9
CTTTACG	45	1.9290383E-8	40.0	1
TCGCGCG	45	1.9290383E-8	40.0	1
TTTGTCG	360	0.0	39.375	1
CCTCGCG	40	3.459245E-7	39.375	1
TTTGGGA	12640	0.0	39.285995	4
TTGGGAC	4670	0.0	38.881157	5
TTTCGCG	545	0.0	38.807343	1
TTGGGAT	8145	0.0	38.75691	5
TACGCGC	70	0.0	38.571426	34
TACGCGG	65	9.094947E-12	38.07692	2
CGCTTGG	545	0.0	37.98165	2
>>END_MODULE