FastQCFastQC Report
Sat 14 Jan 2017
SRR2933947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646988
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC44200.2683686827104994Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41830.25397877823032106No Hit
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT40530.24608558168001224Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC35160.2134806082375828Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC32470.19714776306809764Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG26580.16138551100554469No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC20950.12720189825305345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT21850.041.395881
ACGGGTA556.184564E-1140.9090925
TTTGGGC31850.038.3594974
TTTGGGA87050.038.2538764
TTGGGAC37200.037.7419365
TTGGGCA18350.037.643055
TCGTTTG5500.037.6363641
TTGGGAT49650.037.296075
TGGGCGA17150.037.2594766
TTTCTCG5000.036.91
GGGCGAT24700.036.7105267
TTTCGCG5850.036.5384641
TTGGGAG49450.036.3094025
CGTTTGG10500.036.2142872
TCGCACT250.00210737336.011
CGTTACC250.00210737336.024
GTTACCG751.8189894E-1236.038
ACAATCG250.00210737336.016
GAACCGT250.00210737336.042
TTTACGG2200.035.7954562
Produced by FastQC (version 0.11.5)