##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933947.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1646988
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49836853698995	34.0	31.0	34.0	31.0	34.0
2	32.724736306518324	34.0	31.0	34.0	31.0	34.0
3	32.77550109654715	34.0	31.0	34.0	31.0	34.0
4	36.1326494182107	37.0	35.0	37.0	35.0	37.0
5	36.155679337068634	37.0	35.0	37.0	35.0	37.0
6	35.80428394135234	37.0	35.0	37.0	35.0	37.0
7	36.10448649291919	37.0	35.0	37.0	35.0	37.0
8	36.11892132790281	37.0	37.0	37.0	35.0	37.0
9	37.98161795957226	39.0	39.0	39.0	37.0	39.0
10	37.6780717285129	39.0	37.0	39.0	35.0	39.0
11	37.60198495678171	39.0	37.0	39.0	35.0	39.0
12	37.30027966202547	39.0	37.0	39.0	35.0	39.0
13	37.18932196227295	39.0	37.0	39.0	34.0	39.0
14	38.10877067713912	40.0	38.0	41.0	33.0	41.0
15	38.27203476892363	40.0	38.0	41.0	33.0	41.0
16	38.4485958610506	40.0	38.0	41.0	34.0	41.0
17	38.46743692121618	40.0	38.0	41.0	34.0	41.0
18	38.417670316966486	40.0	37.0	41.0	34.0	41.0
19	38.43177242335706	40.0	37.0	41.0	34.0	41.0
20	38.34125445965605	40.0	37.0	41.0	34.0	41.0
21	38.2754932033506	40.0	37.0	41.0	34.0	41.0
22	38.32718149737582	40.0	37.0	41.0	34.0	41.0
23	38.303121819952544	40.0	37.0	41.0	34.0	41.0
24	38.269389940910315	40.0	37.0	41.0	34.0	41.0
25	38.17180210177609	40.0	36.0	41.0	34.0	41.0
26	38.11440581230707	40.0	36.0	41.0	34.0	41.0
27	38.096823413406774	40.0	36.0	41.0	34.0	41.0
28	38.014193181735386	40.0	36.0	41.0	34.0	41.0
29	37.97235256116013	40.0	36.0	41.0	34.0	41.0
30	37.89964104170765	40.0	36.0	41.0	34.0	41.0
31	37.7848539272903	40.0	36.0	41.0	33.0	41.0
32	37.72719776950409	40.0	36.0	41.0	33.0	41.0
33	37.64820144409067	40.0	35.0	41.0	33.0	41.0
34	37.5116758592048	40.0	35.0	41.0	33.0	41.0
35	37.43915499080746	40.0	35.0	41.0	33.0	41.0
36	37.36117263756627	40.0	35.0	41.0	33.0	41.0
37	37.27454784127146	40.0	35.0	41.0	32.0	41.0
38	37.230693241237944	39.0	35.0	41.0	32.0	41.0
39	37.19437907258583	39.0	35.0	41.0	32.0	41.0
40	37.05820625286887	39.0	35.0	41.0	32.0	41.0
41	36.914307208067086	39.0	35.0	41.0	32.0	41.0
42	36.909843909002376	39.0	35.0	41.0	32.0	41.0
43	36.794421695847205	39.0	35.0	41.0	31.0	41.0
44	36.7081180919351	39.0	35.0	41.0	31.0	41.0
45	36.75576810517138	39.0	35.0	41.0	32.0	41.0
46	36.725215970000995	39.0	35.0	41.0	32.0	41.0
47	36.661242826298675	39.0	35.0	41.0	31.0	41.0
48	36.58445234573658	39.0	35.0	41.0	31.0	41.0
49	36.554095111804095	39.0	35.0	41.0	31.0	41.0
50	36.475475231149225	38.0	35.0	40.0	31.0	41.0
51	35.53200630484254	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	37.0
10	26.0
11	36.0
12	21.0
13	30.0
14	31.0
15	52.0
16	94.0
17	138.0
18	274.0
19	491.0
20	847.0
21	1350.0
22	2094.0
23	3012.0
24	4445.0
25	6557.0
26	9271.0
27	11262.0
28	13305.0
29	15868.0
30	20212.0
31	26312.0
32	35832.0
33	56672.0
34	114190.0
35	116311.0
36	112454.0
37	181690.0
38	340778.0
39	572938.0
40	349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.237942231515955	4.559535345734153	55.06536781081587	16.137154611934026
2	20.416420763235678	4.2597153106154995	53.73348196829607	21.59038195785276
3	21.792872807816448	4.362448299562596	51.463277206634174	22.38140168598678
4	20.23165924706191	4.736585816047232	50.33236429166455	24.699390645226316
5	19.08113477450959	5.6889910551868015	49.525861754912604	25.704012415391002
6	22.520807680444545	6.354205373688211	52.33492897337443	18.790057972492818
7	84.71178903549995	2.29819525096722	8.417122650559689	4.572893062973137
8	86.42801283312326	2.7103415446864214	6.699988099488278	4.161657522702048
9	80.88146361722124	5.21594571423714	8.74511532567329	5.157475342868315
10	41.565512317029636	28.950302005843394	15.595559894789762	13.888625782337211
11	32.44905245211258	23.547833985432803	25.1086832448081	18.894430317646517
12	30.054620920128137	21.318491695142892	28.269787029413695	20.35710035531528
13	28.015929684976452	20.679385642154042	30.864220018603657	20.440464654265845
14	20.899484392114577	22.856632835211915	33.76812702946227	22.47575574321124
15	19.7085831833626	23.56598833749851	35.480707813293115	21.244720665845772
16	26.341175527690545	23.793555265733566	29.152124969945138	20.713144236630747
17	26.375784158718822	23.874794473305208	27.727463709510936	22.021957658465027
18	25.953012408104975	23.137205614127122	29.75522590328527	21.15455607448263
19	26.353076039412553	24.786640825555498	27.84671169431714	21.013571440714806
20	25.205101676514946	28.955645092738987	27.05302042273532	18.786232808010745
21	25.477781258879844	25.25743964133315	30.825543355507147	18.439235744279863
22	23.9162641136426	22.185468260849504	31.924701333585915	21.97356629192198
23	23.044430196212723	23.39519170752914	31.21188496819649	22.34849312806165
24	23.617597699558225	25.06180980067857	28.449205458691868	22.871387041071337
25	24.199326285315983	25.9356473756943	26.32842497941697	23.53660135957275
26	21.791840620575258	26.14390633082937	27.067046025836255	24.99720702275912
27	20.975623380376785	25.35640818269471	30.920565298593555	22.74740313833495
28	19.32709892239652	26.223020447022076	29.073253721338588	25.376626909242813
29	21.913031546070766	26.899649542073167	27.95837006705574	23.228948844800325
30	24.621733734550585	24.407888824933757	28.432569029039676	22.53780841147598
31	23.6260373481774	26.383009469407188	28.412653886974283	21.578299295441134
32	27.062674409285314	25.67826845125769	25.234549371337256	22.024507768119744
33	26.43382951181186	24.908924655188745	26.018708090162164	22.63853774283723
34	22.293544336692193	25.824899756403813	29.116423434779126	22.765132472124872
35	22.710426548341577	27.884599037758623	27.0558133999762	22.3491610139236
36	24.66053183144018	27.39752809370803	25.957869759828245	21.984070315023548
37	23.31279887892322	29.002214952385806	26.319196011142765	21.365790157548204
38	25.15974615479894	26.27675490046072	26.24554641563873	22.31795252910161
39	23.60199345714723	24.54431969146102	26.27772637080537	25.575960480586378
40	25.026168982409104	24.569456486628923	27.22430278787702	23.180071743084955
41	22.795126619016045	25.055009508265996	26.625755621777454	25.524108250940504
42	23.14285228550542	24.153181443944945	28.814660458971165	23.88930581157847
43	22.906056388996156	23.606061489215467	29.446480484375115	24.041401637413266
44	23.395252424425678	23.942797397430944	27.730135252958732	24.93181492518464
45	22.458572861490186	23.442186585451747	26.390416930785165	27.7088236222729
46	21.90459189745159	24.387427230799496	28.31763194388787	25.39034892786104
47	20.238763123957188	25.64256691609168	30.77466259620592	23.344007363745213
48	21.144173485174147	26.220652488056988	29.037734336862197	23.597439689906665
49	24.163260448770725	23.725188040228588	29.21205254683094	22.899498964169744
50	22.07714931742065	24.50017850767583	27.00116819308945	26.421503981814077
51	21.16676017068734	24.560713253527044	26.259693452532744	28.01283312325287
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	342.0
1	1011.0
2	1680.0
3	4460.0
4	7240.0
5	4824.0
6	2408.0
7	2395.5
8	2383.0
9	2489.5
10	2596.0
11	2619.0
12	2642.0
13	2522.0
14	2402.0
15	2486.0
16	2570.0
17	2617.5
18	2665.0
19	2911.0
20	3157.0
21	3757.0
22	4357.0
23	5360.0
24	6363.0
25	7826.0
26	10820.0
27	12351.0
28	15194.0
29	18037.0
30	22328.5
31	26620.0
32	30134.0
33	33648.0
34	37377.0
35	41106.0
36	46483.5
37	51861.0
38	55674.0
39	59487.0
40	65379.5
41	71272.0
42	77325.5
43	83379.0
44	91711.5
45	100044.0
46	113403.0
47	126762.0
48	146722.0
49	166682.0
50	164246.0
51	161810.0
52	146846.0
53	131882.0
54	119558.0
55	107234.0
56	100487.5
57	93741.0
58	89892.5
59	86044.0
60	75746.5
61	65449.0
62	57939.5
63	50430.0
64	43450.0
65	36470.0
66	30930.5
67	25391.0
68	21666.5
69	17942.0
70	14716.0
71	11490.0
72	9774.5
73	8059.0
74	6197.5
75	3613.0
76	2890.0
77	2180.0
78	1470.0
79	1067.0
80	664.0
81	429.5
82	195.0
83	149.5
84	104.0
85	64.0
86	24.0
87	17.5
88	11.0
89	8.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1646988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.281167874210126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.49478189042976	19.231799790086427
2	11.429226483519248	6.2360529273851375
3	4.149903677758395	3.396426566841864
4	2.0793336492216765	2.269066014036421
5	1.217651306207982	1.6609474848455597
6	0.8706032941435694	1.4250644771622634
7	0.6440359900521373	1.2299037773151837
8	0.5096513055537435	1.1123106259297628
9	0.47425796350918015	1.164448000635747
>10	7.76909755035064	51.724910735181986
>50	0.28812796317553474	4.809424489715561
>100	0.06813949614614179	3.387182495132802
>500	0.0024819012718385325	0.4670701326121029
>1k	0.0027075286601874905	1.885392483119229
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	4420	0.2683686827104994	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4183	0.25397877823032106	No Hit
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT	4053	0.24608558168001224	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	3516	0.2134806082375828	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	3247	0.19714776306809764	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2658	0.16138551100554469	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2095	0.12720189825305345	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2143379308167394E-4	0.0	0.0	0.25974688340170055	0.0
2	1.2143379308167394E-4	0.0	0.0	0.9983072129244415	0.0
3	1.2143379308167394E-4	0.0	0.0	1.509057746625962	0.0
4	1.2143379308167394E-4	0.0	0.0	2.0287943810155267	0.0
5	1.2143379308167394E-4	0.0	0.0	2.9662632636060495	0.0
6	1.2143379308167394E-4	0.0	0.0	3.686001355201131	0.0
7	1.2143379308167394E-4	0.0	0.0	4.3150284033642015	0.0
8	1.2143379308167394E-4	0.0	0.0	5.23403934940631	0.0
9	1.2143379308167394E-4	0.0	0.0	5.7410861524188395	0.0
10	1.821506896225109E-4	0.0	0.0	6.612191467090228	0.0
11	1.821506896225109E-4	0.0	0.0	8.157800785433773	0.0
12	1.821506896225109E-4	0.0	0.0	9.452770754856745	0.0
13	1.821506896225109E-4	0.0	0.0	9.872810245126255	0.0
14	1.821506896225109E-4	0.0	0.0	10.076879734400007	0.0
15	1.821506896225109E-4	0.0	0.0	10.328247686079072	0.0
16	1.821506896225109E-4	0.0	0.0	10.863709996672714	0.0
17	1.821506896225109E-4	0.0	0.0	11.547807269998325	0.0
18	1.821506896225109E-4	0.0	0.0	12.267363210903783	0.0
19	1.821506896225109E-4	0.0	0.0	12.813025960116285	0.0
20	1.821506896225109E-4	0.0	0.0	13.464396826206384	0.0
21	1.821506896225109E-4	0.0	0.0	14.316862053639735	0.0
22	1.821506896225109E-4	0.0	0.0	15.259856173815473	0.0
23	1.821506896225109E-4	0.0	0.0	16.099206551596005	0.0
24	1.821506896225109E-4	0.0	0.0	16.73224091493077	0.0
25	1.821506896225109E-4	0.0	0.0	17.330484496547637	0.0
26	1.821506896225109E-4	0.0	0.0	17.851556902661102	0.0
27	1.821506896225109E-4	0.0	0.0	18.464858274620095	0.0
28	1.821506896225109E-4	0.0	0.0	19.023575156588876	0.0
29	1.821506896225109E-4	0.0	0.0	19.677981867506016	0.0
30	1.821506896225109E-4	0.0	0.0	20.45090796047087	0.0
31	1.821506896225109E-4	0.0	0.0	21.051094482777046	0.0
32	1.821506896225109E-4	0.0	0.0	21.612786492676328	0.0
33	1.821506896225109E-4	0.0	0.0	22.178607251540388	0.0
34	1.821506896225109E-4	0.0	0.0	22.80915222211698	0.0
35	1.821506896225109E-4	0.0	0.0	23.46580545820613	0.0
36	1.821506896225109E-4	0.0	0.0	24.04540895258496	0.0
37	1.821506896225109E-4	0.0	0.0	24.656342365578862	0.0
38	1.821506896225109E-4	0.0	0.0	25.38379150303463	0.0
39	1.821506896225109E-4	0.0	0.0	26.748585903479565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	2185	0.0	41.39588	1
ACGGGTA	55	6.184564E-11	40.909092	5
TTTGGGC	3185	0.0	38.359497	4
TTTGGGA	8705	0.0	38.253876	4
TTGGGAC	3720	0.0	37.741936	5
TTGGGCA	1835	0.0	37.64305	5
TCGTTTG	550	0.0	37.636364	1
TTGGGAT	4965	0.0	37.29607	5
TGGGCGA	1715	0.0	37.259476	6
TTTCTCG	500	0.0	36.9	1
GGGCGAT	2470	0.0	36.710526	7
TTTCGCG	585	0.0	36.538464	1
TTGGGAG	4945	0.0	36.309402	5
CGTTTGG	1050	0.0	36.214287	2
TCGCACT	25	0.002107373	36.0	11
CGTTACC	25	0.002107373	36.0	24
GTTACCG	75	1.8189894E-12	36.0	38
ACAATCG	25	0.002107373	36.0	16
GAACCGT	25	0.002107373	36.0	42
TTTACGG	220	0.0	35.795456	2
>>END_MODULE