SRR2933946.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933946.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1610088
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	4767	0.29607077377137153	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	4508	0.2799846964886391	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	4214	0.26172482497851046	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	3865	0.24004899111104488	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3326	0.20657256000914237	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3121	0.19384033667724992	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2667	0.16564312012759552	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1702	0.1057085078579556	No Hit
TCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1627	0.10105037737067789	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACATCG	20	7.03413E-4	45.0	31
CGTTTTT	1935	0.0	40.0	1
ACGATAC	40	3.4593722E-7	39.375	45
TTTGGGC	3215	0.0	39.19129	4
TGGGCGA	1645	0.0	38.57143	6
TAACGGG	35	6.249851E-6	38.571426	3
TTTCGCG	550	0.0	38.454544	1
TTTGGGA	8710	0.0	37.766933	4
GCGATGT	370	0.0	37.7027	9
TTGGGAC	3425	0.0	37.248177	5
CTACGAA	145	0.0	37.24138	11
CGTTTGG	1205	0.0	37.157677	2
ACATACG	170	0.0	37.058823	17
TGCGTTG	280	0.0	36.964283	1
TTACGGG	305	0.0	36.885246	3
CGAACGA	55	2.750312E-9	36.81818	22
GATAACG	55	2.750312E-9	36.81818	17
TACGGGT	55	2.750312E-9	36.81818	4
TTGGGAG	4840	0.0	36.585747	5
GGGCGAT	2510	0.0	36.573704	7