##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933946.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1610088
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41484564818818	33.0	31.0	34.0	31.0	34.0
2	32.640654423857576	34.0	31.0	34.0	31.0	34.0
3	32.70079958362524	34.0	31.0	34.0	31.0	34.0
4	36.073887265789196	37.0	35.0	37.0	35.0	37.0
5	36.09386319257084	37.0	35.0	37.0	35.0	37.0
6	35.737469007905155	37.0	35.0	37.0	35.0	37.0
7	36.06043955361446	37.0	35.0	37.0	35.0	37.0
8	36.076034974485864	37.0	36.0	37.0	35.0	37.0
9	37.9287865011105	39.0	38.0	39.0	35.0	39.0
10	37.60031811925808	39.0	37.0	39.0	35.0	39.0
11	37.501249000054656	39.0	37.0	39.0	35.0	39.0
12	37.19002625943427	39.0	37.0	39.0	34.0	39.0
13	37.05040035078828	39.0	37.0	39.0	33.0	39.0
14	37.90481017186638	40.0	37.0	41.0	33.0	41.0
15	38.066004466836596	40.0	37.0	41.0	33.0	41.0
16	38.25785547125375	40.0	37.0	41.0	33.0	41.0
17	38.279616393638115	40.0	37.0	41.0	34.0	41.0
18	38.24183398671377	40.0	37.0	41.0	34.0	41.0
19	38.25971623911239	40.0	37.0	41.0	34.0	41.0
20	38.162490497413806	40.0	37.0	41.0	34.0	41.0
21	38.10278506516414	40.0	36.0	41.0	34.0	41.0
22	38.152687306532314	40.0	36.0	41.0	34.0	41.0
23	38.13568513025375	40.0	36.0	41.0	34.0	41.0
24	38.09052300246943	40.0	36.0	41.0	34.0	41.0
25	37.99179920600613	40.0	36.0	41.0	34.0	41.0
26	37.92415445615395	40.0	36.0	41.0	34.0	41.0
27	37.92356194195597	40.0	36.0	41.0	34.0	41.0
28	37.85608302154913	40.0	36.0	41.0	34.0	41.0
29	37.807882550518976	40.0	36.0	41.0	34.0	41.0
30	37.734588420011825	40.0	35.0	41.0	33.0	41.0
31	37.62411930279587	40.0	35.0	41.0	33.0	41.0
32	37.54162753837058	40.0	35.0	41.0	33.0	41.0
33	37.50754430813719	40.0	35.0	41.0	33.0	41.0
34	37.41009311292302	40.0	35.0	41.0	33.0	41.0
35	37.33324327614391	40.0	35.0	41.0	32.0	41.0
36	37.27616751382533	40.0	35.0	41.0	32.0	41.0
37	37.18786985556069	39.0	35.0	41.0	32.0	41.0
38	37.11668306328598	39.0	35.0	41.0	32.0	41.0
39	37.06833166882804	39.0	35.0	41.0	32.0	41.0
40	36.972074197186735	39.0	35.0	41.0	32.0	41.0
41	36.810855058853925	39.0	35.0	41.0	31.0	41.0
42	36.79684091801194	39.0	35.0	41.0	31.0	41.0
43	36.73371952340493	39.0	35.0	41.0	31.0	41.0
44	36.662370628189265	39.0	35.0	41.0	31.0	41.0
45	36.6828024306746	39.0	35.0	41.0	31.0	41.0
46	36.64384120619494	39.0	35.0	41.0	31.0	41.0
47	36.566292028758674	39.0	35.0	41.0	31.0	41.0
48	36.4678284665186	38.0	35.0	40.0	31.0	41.0
49	36.45872151087394	38.0	35.0	40.0	31.0	41.0
50	36.38074005892845	38.0	35.0	40.0	31.0	41.0
51	35.450792751700526	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	18.0
10	49.0
11	32.0
12	31.0
13	19.0
14	26.0
15	40.0
16	84.0
17	180.0
18	291.0
19	500.0
20	866.0
21	1396.0
22	2025.0
23	3282.0
24	4707.0
25	6838.0
26	9356.0
27	11730.0
28	13548.0
29	16617.0
30	20912.0
31	27503.0
32	38282.0
33	59013.0
34	120084.0
35	117336.0
36	112736.0
37	180063.0
38	333949.0
39	528284.0
40	282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.50605184312907	4.6081332200476	55.32933603629119	15.556478900532145
2	20.655641182345313	4.476587615086877	53.421614222328216	21.44615698023959
3	21.644096471745645	4.6213623106314685	50.6623240468844	23.072217170738494
4	20.263550812129523	4.732163707822181	50.68027337636204	24.324012103686258
5	19.31279532547289	5.774342768842448	49.67958273088179	25.23327917480287
6	22.07730260706247	6.24350967152106	52.63258902618987	19.046598695226596
7	83.86299382394006	2.275714122457903	9.114657087066048	4.74663496653599
8	85.8841255881666	2.859222601497558	6.949061169327391	4.307590641008441
9	80.27480485538678	5.108913301633203	9.221669871460442	5.394611971519569
10	42.28284416752376	27.469989217980633	16.2007294011259	14.046437213369703
11	32.679207596106544	23.56063767943119	24.772310581781866	18.987844142680398
12	30.511810534579475	20.501798659452152	28.471984139997318	20.514406665971052
13	28.37223803916308	20.234546186295407	31.0142675431405	20.37894823140102
14	21.482676723259846	22.196861289569267	33.99870069213608	22.321761295034808
15	19.620977238511188	23.719510983250604	35.75630648759571	20.9032052906425
16	26.29595400996716	23.539769254848185	29.26635065909441	20.89792607609025
17	26.413338898246558	23.49349849200789	27.93878347022026	22.154379139525293
18	26.177451170370812	22.8541545555274	29.91221597825709	21.0561782958447
19	26.551902753141444	24.873982043217513	27.66184208564998	20.912273117991067
20	24.612505651864993	29.373115009862815	27.381360521909357	18.63301881636283
21	25.574068001252105	24.80802291551766	31.177985302666688	18.439923780563547
22	23.116189922538396	22.71732973601443	32.12830603047784	22.03817431096934
23	22.83235450484694	23.512441555989486	31.156930552864193	22.498273386299385
24	23.938691549778646	24.892366131540637	28.233239425422713	22.935702893258007
25	24.647286359503333	25.184896726141677	25.7166689025693	24.45114801178569
26	20.965934781204506	25.21682044708115	26.9866615986207	26.830583173093647
27	20.85358067385137	24.348979683098065	30.16717098692742	24.630268656123143
28	18.751459547552678	24.944288759372157	28.294167772196303	28.01008392087886
29	20.88115680633605	25.950072294185162	27.59414392256821	25.57462697691058
30	24.04502114170157	23.548774973790252	28.38062267404018	24.025581210468
31	22.96862034870144	24.73262331003026	28.53017971688504	23.768576624383265
32	25.170673901053853	24.622318780091522	26.376943372039293	23.83006394681533
33	25.39084820208585	22.94719294845996	27.739601810584265	23.922357038869926
34	21.24039182951491	23.096687882898326	31.08451215088865	24.57840813669812
35	20.57061477385087	25.96019596444418	28.42596181078301	25.04322745092194
36	23.184384952872144	24.581140906583986	27.1437337586517	25.090740381892168
37	21.727011194419187	26.72065129359389	27.373969621536215	24.17836789045071
38	22.44181684479358	25.791323207178742	27.992383025027202	23.77447692300048
39	21.746389017246262	24.253519062312122	29.230389891732628	24.76970202870899
40	24.692066520587694	22.171707384937967	30.168102613024878	22.968123481449464
41	21.70049090484495	22.52199879758125	28.940281524984968	26.83722877258883
42	19.813575407058494	22.556531071593604	31.647524855784283	25.98236866556362
43	20.16684802321364	22.281328722405235	31.209412156354187	26.34241109802694
44	21.66049309106086	22.385360303287772	28.880781671560808	27.07336493409056
45	21.27039018985298	21.87718932132902	27.035603022940364	29.816817465877644
46	20.699303392112732	22.385857170539747	29.380071151390485	27.53476828595704
47	18.597803349879012	23.372138665712683	32.231033334823934	25.799024649584368
48	19.243606560635197	24.77411172557028	30.565223764166927	25.417057949627598
49	22.183880632611384	22.267043788910918	30.856325865418537	24.69274971305916
50	20.434721580435355	22.840614922911044	29.460315212584653	27.264348284068944
51	19.77655879678626	22.560133359170432	28.15001416071668	29.513293683326623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	205.0
1	868.0
2	1531.0
3	3727.5
4	5924.0
5	3974.0
6	2024.0
7	2142.5
8	2261.0
9	2296.5
10	2332.0
11	2367.5
12	2403.0
13	2348.0
14	2293.0
15	2265.0
16	2237.0
17	2366.0
18	2495.0
19	2625.0
20	2755.0
21	3438.5
22	4122.0
23	5045.5
24	5969.0
25	7393.0
26	10591.0
27	12365.0
28	14438.0
29	16511.0
30	20786.0
31	25061.0
32	28797.5
33	32534.0
34	36362.0
35	40190.0
36	42596.5
37	45003.0
38	49549.5
39	54096.0
40	59509.0
41	64922.0
42	70709.5
43	76497.0
44	85392.5
45	94288.0
46	110970.0
47	127652.0
48	147224.0
49	166796.0
50	169198.5
51	171601.0
52	152462.5
53	133324.0
54	122050.5
55	110777.0
56	103464.5
57	96152.0
58	90562.0
59	84972.0
60	74959.0
61	64946.0
62	55767.5
63	46589.0
64	40587.0
65	34585.0
66	29569.5
67	24554.0
68	19932.0
69	15310.0
70	12841.0
71	10372.0
72	8819.0
73	7266.0
74	5750.5
75	3198.5
76	2162.0
77	1645.5
78	1129.0
79	765.5
80	402.0
81	332.5
82	263.0
83	187.5
84	112.0
85	64.0
86	16.0
87	22.0
88	28.0
89	17.0
90	6.0
91	5.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1610088.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.729271482158257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.47226215928886	20.24616751536038
2	11.64153397555317	6.689055801048722
3	4.420882055418719	3.810261622821786
4	2.1763161265092315	2.500959073179312
5	1.3165983589354615	1.8912455843410458
6	0.8759065276766514	1.509849385597025
7	0.6505591373214279	1.3083063049914183
8	0.5297483977227864	1.217542842833302
9	0.4407283899792111	1.1395625009046544
>10	7.175925952326859	49.012449942129656
>50	0.2176970138732528	3.8860029721499094
>100	0.07418290682993321	3.746861626942948
>500	0.004814227669114145	0.8798983247935652
>1k	0.002844770895385631	2.161836502906282
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	4767	0.29607077377137153	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	4508	0.2799846964886391	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	4214	0.26172482497851046	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	3865	0.24004899111104488	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3326	0.20657256000914237	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	3121	0.19384033667724992	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2667	0.16564312012759552	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1702	0.1057085078579556	No Hit
TCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1627	0.10105037737067789	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.210840649703618E-5	0.0	0.0	0.2770034929767814	0.0
2	6.210840649703618E-5	0.0	0.0	1.1623588275920322	0.0
3	6.210840649703618E-5	0.0	0.0	1.7418302602093798	0.0
4	6.210840649703618E-5	0.0	0.0	2.3608026393588424	0.0
5	6.210840649703618E-5	0.0	0.0	3.4421721048787397	0.0
6	6.210840649703618E-5	0.0	0.0	4.226414953716816	0.0
7	6.210840649703618E-5	0.0	0.0	4.931407475864673	0.0
8	6.210840649703618E-5	0.0	0.0	5.963649191845414	0.0
9	6.210840649703618E-5	0.0	0.0	6.511072686710292	0.0
10	6.210840649703618E-5	0.0	0.0	7.468846423301087	0.0
11	6.210840649703618E-5	0.0	0.0	9.203472108356811	0.0
12	6.210840649703618E-5	0.0	0.0	10.718482468039014	0.0
13	6.210840649703618E-5	0.0	0.0	11.225783932306806	0.0
14	6.210840649703618E-5	0.0	0.0	11.466950874734797	0.0
15	6.210840649703618E-5	0.0	0.0	11.760785745872274	0.0
16	6.210840649703618E-5	0.0	0.0	12.350939824407114	0.0
17	6.210840649703618E-5	0.0	0.0	13.13369207148926	0.0
18	6.210840649703618E-5	0.0	0.0	13.958678034989392	0.0
19	6.210840649703618E-5	0.0	0.0	14.584358122040534	0.0
20	6.210840649703618E-5	0.0	0.0	15.306119913942592	0.0
21	6.210840649703618E-5	0.0	0.0	16.30047550196014	0.0
22	6.210840649703618E-5	0.0	0.0	17.371907622440514	0.0
23	6.210840649703618E-5	0.0	0.0	18.326451721893463	0.0
24	6.210840649703618E-5	0.0	0.0	19.067156577777116	0.0
25	6.210840649703618E-5	0.0	0.0	19.74972796517954	0.0
26	6.210840649703618E-5	0.0	0.0	20.37640178673464	0.0
27	6.210840649703618E-5	0.0	0.0	21.07897208102911	0.0
28	6.210840649703618E-5	0.0	0.0	21.702913132698338	0.0
29	6.210840649703618E-5	0.0	0.0	22.439146183314204	0.0
30	6.210840649703618E-5	0.0	0.0	23.334128320936497	0.0
31	6.210840649703618E-5	0.0	0.0	23.992601646618073	0.0
32	6.210840649703618E-5	0.0	0.0	24.630020222497155	0.0
33	6.210840649703618E-5	0.0	0.0	25.270233676668603	0.0
34	6.210840649703618E-5	0.0	0.0	26.01031744848729	0.0
35	6.210840649703618E-5	0.0	0.0	26.75418983310229	0.0
36	6.210840649703618E-5	0.0	0.0	27.419370866685547	0.0
37	6.210840649703618E-5	0.0	0.0	28.110513214184568	0.0
38	6.210840649703618E-5	0.0	0.0	28.936430803782155	0.0
39	1.2421681299407236E-4	0.0	0.0	30.519822519017595	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACATCG	20	7.03413E-4	45.0	31
CGTTTTT	1935	0.0	40.0	1
ACGATAC	40	3.4593722E-7	39.375	45
TTTGGGC	3215	0.0	39.19129	4
TGGGCGA	1645	0.0	38.57143	6
TAACGGG	35	6.249851E-6	38.571426	3
TTTCGCG	550	0.0	38.454544	1
TTTGGGA	8710	0.0	37.766933	4
GCGATGT	370	0.0	37.7027	9
TTGGGAC	3425	0.0	37.248177	5
CTACGAA	145	0.0	37.24138	11
CGTTTGG	1205	0.0	37.157677	2
ACATACG	170	0.0	37.058823	17
TGCGTTG	280	0.0	36.964283	1
TTACGGG	305	0.0	36.885246	3
CGAACGA	55	2.750312E-9	36.81818	22
GATAACG	55	2.750312E-9	36.81818	17
TACGGGT	55	2.750312E-9	36.81818	4
TTGGGAG	4840	0.0	36.585747	5
GGGCGAT	2510	0.0	36.573704	7
>>END_MODULE