SRR2933945.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933945.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	539633
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	5250	0.972883422622412	RNA PCR Primer, Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	3556	0.6589663715895804	TruSeq Adapter, Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2693	0.49904286802326764	Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2548	0.47217275444607726	Illumina PCR Primer Index 3 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	1065	0.19735635144626068	TruSeq Adapter, Index 7 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	1059	0.19624448467754937	TruSeq Adapter, Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	997	0.184755194734199	No Hit
GCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	736	0.13638899029525622	Illumina PCR Primer Index 3 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	709	0.13138558983605525	TruSeq Adapter, Index 7 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT	568	0.10525672077133905	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	556	0.10303298723391638	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	25	3.8880447E-5	45.000004	19
GCGAAGT	30	2.1633532E-6	45.000004	1
CGACCGA	25	3.8880447E-5	45.000004	25
TCTAGCG	25	3.8880447E-5	45.000004	1
GACCATA	25	3.8880447E-5	45.000004	9
AATGCGC	25	3.8880447E-5	45.000004	24
CGCTCTA	25	3.8880447E-5	45.000004	28
ACTAGCG	25	3.8880447E-5	45.000004	18
TAACCCG	25	3.8880447E-5	45.000004	18
GTCACTA	30	2.1633532E-6	45.000004	25
CGCTAAT	25	3.8880447E-5	45.000004	15
TATTAGG	25	3.8880447E-5	45.000004	2
CTCGCTA	25	3.8880447E-5	45.000004	13
TATAGTG	25	3.8880447E-5	45.000004	38
ACGACCG	25	3.8880447E-5	45.000004	24
TTTAGCG	70	0.0	45.0	1
CGGGTAT	80	0.0	45.0	6
CGGGTAC	35	1.2103192E-7	45.0	6
TCGCAAC	20	7.0299575E-4	45.0	44
ATAGGGT	20	7.0299575E-4	45.0	24