FastQCFastQC Report
Sat 14 Jan 2017
SRR2933944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336144
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT142511.0665766564082912TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC137311.0276586954699494TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC114050.8535756625034427TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC96060.7189344861032942TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG41600.3113436875067358TruSeq Adapter, Index 20 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG38920.2912859691769749TruSeq Adapter, Index 20 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG29560.2212336394879594No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCC27600.20656456190350742No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC26210.1961614915757583TruSeq Adapter, Index 20 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG25300.18935084841154845TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG24160.1808188338981427TruSeq Adapter, Index 20 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT24060.1800704115724054No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22100.1654013339879534No Hit
TTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG19260.14414613993701278TruSeq Adapter, Index 20 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT17930.1341921230047061TruSeq Adapter, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT351.21212E-745.00000425
TATCGTG351.21212E-745.0000041
CGTTAGT253.8911476E-545.03
CGAACGA302.1657688E-644.99999622
TACGTAC302.1657688E-644.99999626
TTACCGG800.042.18752
TATTGCG700.041.7857171
CGTTTTT17400.040.0862051
TTTGTCG4300.039.767441
GATCGGA403.4587902E-739.3759
CTTTGCG4200.039.1071431
TACGAAT700.038.5714312
TTAGTCG356.249051E-638.571431
TACGGTT659.094947E-1238.0769233
GGCGATA659.094947E-1238.076928
ACGGTTA659.094947E-1238.0769234
GCCGATT1600.037.968759
TTTGGGA56900.037.921794
TTGGGAC20650.037.8087165
TTTCGCG5200.037.644231
Produced by FastQC (version 0.11.5)