##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933944.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1336144
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29675469111114	33.0	31.0	34.0	31.0	34.0
2	32.52170275060173	34.0	31.0	34.0	31.0	34.0
3	32.59314490054964	34.0	31.0	34.0	31.0	34.0
4	35.99418026799507	37.0	35.0	37.0	35.0	37.0
5	36.02680773928559	37.0	35.0	37.0	35.0	37.0
6	35.6730883796956	37.0	35.0	37.0	35.0	37.0
7	35.9717784909411	37.0	35.0	37.0	35.0	37.0
8	35.979193859344505	37.0	35.0	37.0	35.0	37.0
9	37.828142026607914	39.0	38.0	39.0	35.0	39.0
10	37.52389787328312	39.0	37.0	39.0	35.0	39.0
11	37.456808547581694	39.0	37.0	39.0	35.0	39.0
12	37.16445607659055	39.0	37.0	39.0	34.0	39.0
13	37.00484977667078	39.0	37.0	39.0	33.0	39.0
14	37.800919661353866	40.0	37.0	41.0	33.0	41.0
15	37.94380994862829	40.0	37.0	41.0	33.0	41.0
16	38.145072686776274	40.0	37.0	41.0	33.0	41.0
17	38.1939177214432	40.0	37.0	41.0	33.0	41.0
18	38.168693643798875	40.0	37.0	41.0	34.0	41.0
19	38.22210480307512	40.0	37.0	41.0	34.0	41.0
20	38.129905159922885	40.0	37.0	41.0	33.0	41.0
21	38.060227041396736	40.0	36.0	41.0	33.0	41.0
22	38.11775751715384	40.0	36.0	41.0	34.0	41.0
23	38.103022578404726	40.0	36.0	41.0	34.0	41.0
24	38.07281325964866	40.0	36.0	41.0	34.0	41.0
25	37.96826988707804	40.0	36.0	41.0	34.0	41.0
26	37.915634093331256	40.0	36.0	41.0	34.0	41.0
27	37.86954250440073	40.0	36.0	41.0	34.0	41.0
28	37.812314391263214	40.0	36.0	41.0	34.0	41.0
29	37.766763163251866	40.0	36.0	41.0	34.0	41.0
30	37.6749437186411	40.0	36.0	41.0	33.0	41.0
31	37.59927971835371	40.0	36.0	41.0	33.0	41.0
32	37.559595372953815	40.0	35.0	41.0	33.0	41.0
33	37.5138532972494	40.0	35.0	41.0	33.0	41.0
34	37.47028164628962	40.0	35.0	41.0	33.0	41.0
35	37.41746997329629	40.0	35.0	41.0	33.0	41.0
36	37.304919978684936	40.0	35.0	41.0	32.0	41.0
37	37.206982929983596	39.0	35.0	41.0	32.0	41.0
38	37.15889829239962	39.0	35.0	41.0	32.0	41.0
39	37.14101249565915	39.0	35.0	41.0	32.0	41.0
40	37.02187413931433	39.0	35.0	41.0	32.0	41.0
41	36.89060610233628	39.0	35.0	41.0	31.0	41.0
42	36.93060703037996	39.0	35.0	41.0	31.0	41.0
43	36.84369124884743	39.0	35.0	41.0	31.0	41.0
44	36.718039373001716	39.0	35.0	41.0	31.0	41.0
45	36.74892302027327	39.0	35.0	41.0	31.0	41.0
46	36.704049114466706	39.0	35.0	41.0	31.0	41.0
47	36.630081787591756	39.0	35.0	40.0	31.0	41.0
48	36.58417131686405	39.0	35.0	40.0	31.0	41.0
49	36.59230367385551	39.0	35.0	40.0	31.0	41.0
50	36.514417607683	39.0	35.0	40.0	31.0	41.0
51	35.61086978649008	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	28.0
10	43.0
11	35.0
12	19.0
13	26.0
14	35.0
15	49.0
16	62.0
17	156.0
18	244.0
19	425.0
20	690.0
21	1152.0
22	1746.0
23	2564.0
24	3843.0
25	5466.0
26	7210.0
27	9197.0
28	11113.0
29	14022.0
30	17762.0
31	23737.0
32	33008.0
33	50899.0
34	97631.0
35	93512.0
36	95740.0
37	151420.0
38	288542.0
39	425541.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.039890909961798	4.630114718174089	53.36026655809554	16.969727813768575
2	20.972589780742197	4.331419367972314	51.24919170388821	23.446799147397286
3	21.73343591708678	4.512986624196194	50.818250128728636	22.935327329988382
4	21.146597971476126	4.457528529859057	49.2337652229101	25.162108275754708
5	19.0768360296495	5.288576680357806	49.1140924930247	26.52049479696799
6	21.840759678597514	6.0438844914919345	51.85893137266642	20.25642445724413
7	78.23228634039445	2.563047096720114	12.418796177657498	6.785870385227939
8	78.19321869499096	3.079683028176604	10.8336376917458	7.893460585086637
9	72.43515668969812	5.416781424756613	14.232971895244823	7.915089990300446
10	38.21841059047528	26.61464632554575	20.782789878935205	14.384153205043768
11	30.605758062005293	23.13904788705409	27.22363757199823	19.03155647894239
12	28.533975379899175	20.73159779185477	31.189377791615275	19.545049036630783
13	27.226631261301176	22.005861643655177	31.644493407896157	19.123013687147495
14	19.759546875187105	24.20195727406627	33.84410662323821	22.194389227508413
15	17.032445604665366	24.867304721646768	36.741997868493215	21.35825180519465
16	23.341496126166042	25.369870313379394	28.558897843346227	22.729735717108333
17	23.43931492413991	24.890206444814332	27.596726101378295	24.073752529667463
18	23.082242632530626	23.56153228993282	31.396615933611944	21.95960914392461
19	23.585781173286712	25.114358931372667	28.660009699553342	22.639850195787282
20	22.651525584068782	29.441886503251148	29.339801698020572	18.566786214659498
21	23.894879593816235	26.10946125567304	31.637607922499374	18.35805122801135
22	20.84880072806524	24.471464153564288	31.93203726544444	22.747697852926034
23	20.96360871283335	24.14462812391479	31.178151456729214	23.71361170652265
24	23.322186830162018	24.897915194769425	27.548078650205365	24.23181932486319
25	22.384563340478273	25.01833634698056	25.462450155073107	27.13465015746806
26	19.09285226742028	25.782475541558398	25.839205953849287	29.28546623717204
27	20.09192123004706	26.60806020907926	29.07598282819816	24.22403573267552
28	18.79168712354357	25.671185141721253	26.782517453208637	28.754610281526542
29	20.12073548958795	25.944059921685085	25.35999113867966	28.5752134500473
30	22.82283945443006	24.2696894945455	26.45972290411812	26.44774814690632
31	21.386392484642375	25.017064029026812	28.87091511094613	24.72562837538469
32	23.01219030284161	24.09313666790406	27.69334742363099	25.20132560562335
33	22.91579350728664	22.983226358835573	29.257924295584907	24.84305583829288
34	18.995108311678983	21.640107653067332	34.10897328431666	25.255810750937023
35	18.317860949119257	24.57841370391215	29.505577243171395	27.598148103797193
36	22.218488426397155	25.33162593253422	28.414826545641787	24.03505909542684
37	20.39076626471398	27.198340897388306	29.890939898693553	22.519952939204156
38	21.374791938593447	25.029637524099197	28.448655234765113	25.14691530254224
39	20.373627393454598	21.839113149480895	30.069588307847056	27.71767114921745
40	22.926421104312112	21.103863056676524	31.10959597169167	24.860119867319693
41	18.148867187967763	22.597639176615694	30.33176064855285	28.92173298686369
42	19.602378186782264	22.032954531846865	32.15267216707181	26.211995114299057
43	21.72699948508544	21.51774060280928	31.001748314552923	25.753511597552357
44	22.088487468416577	20.723589672969382	28.118975200277813	29.068947658336224
45	20.41336861895125	19.975092504999463	27.066768252523683	32.54477062352561
46	20.34279239363422	21.299650337089414	30.78006562166952	27.577491647606845
47	16.6371289322109	23.00358344609563	34.773647151804	25.585640469889476
48	17.512783053323595	23.250787340286674	32.37772276042104	26.8587068459687
49	21.7442131985774	19.994401801003484	33.32821911410746	24.933165886311656
50	20.407830293740794	20.22895735788957	30.272410758121882	29.090801590247757
51	18.22438300063466	20.38590151959669	28.982654564178713	32.40706091558994
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	348.0
1	775.5
2	1203.0
3	2558.0
4	3913.0
5	2779.5
6	1646.0
7	1834.0
8	2022.0
9	2373.0
10	2724.0
11	2662.5
12	2601.0
13	2686.5
14	2772.0
15	2829.5
16	2887.0
17	2852.5
18	2818.0
19	2851.0
20	2884.0
21	3247.0
22	3610.0
23	4589.5
24	5569.0
25	6418.0
26	8683.0
27	10099.0
28	11493.0
29	12887.0
30	15042.5
31	17198.0
32	20570.5
33	23943.0
34	26920.5
35	29898.0
36	31908.0
37	33918.0
38	37019.5
39	40121.0
40	44971.0
41	49821.0
42	56347.0
43	62873.0
44	72558.5
45	82244.0
46	100768.5
47	119293.0
48	150397.0
49	181501.0
50	176040.5
51	170580.0
52	140074.5
53	109569.0
54	98757.5
55	87946.0
56	80557.0
57	73168.0
58	67252.5
59	61337.0
60	51514.5
61	41692.0
62	36075.0
63	30458.0
64	25091.5
65	19725.0
66	16471.5
67	13218.0
68	11232.5
69	9247.0
70	7360.0
71	5473.0
72	4476.5
73	3480.0
74	2726.0
75	1599.5
76	1227.0
77	916.0
78	605.0
79	419.5
80	234.0
81	161.0
82	88.0
83	66.5
84	45.0
85	27.5
86	10.0
87	7.0
88	4.0
89	4.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1336144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.389687508460415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.45840253210737	20.413607452554913
2	12.980940964697494	7.630115970364641
3	4.883650000658576	4.305868422601441
4	2.3853574623469025	2.804196416573984
5	1.3412986944347232	1.971017474247122
6	0.9040311322926398	1.5941515485600195
7	0.6084410070608894	1.2517323745396751
8	0.5247491538377378	1.233777092129611
9	0.4229149786578565	1.1186405158861445
>10	6.214039465143186	41.0793821992166
>50	0.1773430068428434	3.4715334624958385
>100	0.08544815588804343	4.840444688068004
>500	0.006691723015901936	1.3545862485781288
>1k	0.005662227167301638	3.2250468670078796
>5k	2.573739621500745E-4	0.7266113191781052
>10k+	7.721218864502235E-4	2.979287947997921
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	14251	1.0665766564082912	TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	13731	1.0276586954699494	TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	11405	0.8535756625034427	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	9606	0.7189344861032942	TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	4160	0.3113436875067358	TruSeq Adapter, Index 20 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	3892	0.2912859691769749	TruSeq Adapter, Index 20 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2956	0.2212336394879594	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCC	2760	0.20656456190350742	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	2621	0.1961614915757583	TruSeq Adapter, Index 20 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	2530	0.18935084841154845	TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	2416	0.1808188338981427	TruSeq Adapter, Index 20 (95% over 23bp)
TTCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	2406	0.1800704115724054	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2210	0.1654013339879534	No Hit
TTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	1926	0.14414613993701278	TruSeq Adapter, Index 20 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	1793	0.1341921230047061	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9936893029493825E-4	0.0	0.0	1.1511483792166113	0.0
2	2.9936893029493825E-4	0.0	0.0	4.007053131997749	0.0
3	2.9936893029493825E-4	0.0	0.0	5.600219736794837	0.0
4	2.9936893029493825E-4	0.0	0.0	7.115325893017519	0.0
5	2.9936893029493825E-4	0.0	0.0	9.471134847740963	0.0
6	2.9936893029493825E-4	0.0	0.0	10.790378881318182	0.0
7	2.9936893029493825E-4	0.0	0.0	11.955223389095787	0.0
8	2.9936893029493825E-4	0.0	0.0	13.541579350728664	0.0
9	2.9936893029493825E-4	0.0	0.0	14.266501215437858	0.0
10	2.9936893029493825E-4	0.0	0.0	15.628779532744973	0.0
11	2.9936893029493825E-4	0.0	0.0	18.1199780861943	0.0
12	2.9936893029493825E-4	0.0	0.0	20.21413859583997	0.0
13	2.9936893029493825E-4	0.0	0.0	20.989129914140992	0.0
14	2.9936893029493825E-4	0.0	0.0	21.339092193655773	0.0
15	2.9936893029493825E-4	0.0	0.0	21.79233675412231	0.0
16	2.9936893029493825E-4	0.0	0.0	22.74485384808823	0.0
17	2.9936893029493825E-4	0.0	0.0	23.968000455040773	0.0
18	2.9936893029493825E-4	0.0	0.0	25.265914452334478	0.0
19	2.9936893029493825E-4	0.0	0.0	26.15167227484463	0.0
20	2.9936893029493825E-4	0.0	0.0	27.150816079703983	0.0
21	2.9936893029493825E-4	0.0	0.0	28.355027601815372	0.0
22	2.9936893029493825E-4	0.0	0.0	29.56904345639392	0.0
23	2.9936893029493825E-4	0.0	0.0	30.600968159120573	0.0
24	2.9936893029493825E-4	0.0	0.0	31.413156067010743	0.0
25	2.9936893029493825E-4	0.0	0.0	32.16479660874876	0.0
26	2.9936893029493825E-4	0.0	0.0	32.85334514842712	0.0
27	2.9936893029493825E-4	0.0	0.0	33.65647714617586	0.0
28	2.9936893029493825E-4	0.0	0.0	34.33050629273492	0.0
29	2.9936893029493825E-4	0.0	0.0	35.10841645810631	0.0
30	2.9936893029493825E-4	0.0	0.0	35.950765785723696	0.0
31	2.9936893029493825E-4	0.0	0.0	36.641185381216395	0.0
32	2.9936893029493825E-4	0.0	0.0	37.28108646972183	0.0
33	2.9936893029493825E-4	0.0	0.0	37.928995677112646	0.0
34	2.9936893029493825E-4	0.0	0.0	38.61020967799878	0.0
35	2.9936893029493825E-4	0.0	0.0	39.31522350884336	0.0
36	2.9936893029493825E-4	0.0	0.0	39.90812367529249	0.0
37	2.9936893029493825E-4	0.0	0.0	40.550868768635716	0.0
38	3.742111628686728E-4	0.0	0.0	41.26740830329665	0.0
39	3.742111628686728E-4	0.0	0.0	42.406058029673446	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAT	35	1.21212E-7	45.000004	25
TATCGTG	35	1.21212E-7	45.000004	1
CGTTAGT	25	3.8911476E-5	45.0	3
CGAACGA	30	2.1657688E-6	44.999996	22
TACGTAC	30	2.1657688E-6	44.999996	26
TTACCGG	80	0.0	42.1875	2
TATTGCG	70	0.0	41.785717	1
CGTTTTT	1740	0.0	40.086205	1
TTTGTCG	430	0.0	39.76744	1
GATCGGA	40	3.4587902E-7	39.375	9
CTTTGCG	420	0.0	39.107143	1
TACGAAT	70	0.0	38.57143	12
TTAGTCG	35	6.249051E-6	38.57143	1
TACGGTT	65	9.094947E-12	38.07692	33
GGCGATA	65	9.094947E-12	38.07692	8
ACGGTTA	65	9.094947E-12	38.07692	34
GCCGATT	160	0.0	37.96875	9
TTTGGGA	5690	0.0	37.92179	4
TTGGGAC	2065	0.0	37.808716	5
TTTCGCG	520	0.0	37.64423	1
>>END_MODULE