##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933942.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1125451
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43707278237791	33.0	31.0	34.0	31.0	34.0
2	32.665630933732345	34.0	31.0	34.0	31.0	34.0
3	32.72712539239825	34.0	31.0	34.0	31.0	34.0
4	36.09163082177722	37.0	35.0	37.0	35.0	37.0
5	36.12977019879142	37.0	35.0	37.0	35.0	37.0
6	35.78423671932408	37.0	35.0	37.0	35.0	37.0
7	36.08885859979688	37.0	35.0	37.0	35.0	37.0
8	36.09069697392423	37.0	37.0	37.0	35.0	37.0
9	37.94223382448458	39.0	38.0	39.0	35.0	39.0
10	37.64599791550232	39.0	37.0	39.0	35.0	39.0
11	37.600289128536026	39.0	37.0	39.0	35.0	39.0
12	37.37491992099167	39.0	37.0	39.0	35.0	39.0
13	37.26069993273808	39.0	37.0	39.0	34.0	39.0
14	38.4129553396816	40.0	38.0	41.0	34.0	41.0
15	38.50132080383775	40.0	38.0	41.0	34.0	41.0
16	38.58991550942689	40.0	38.0	41.0	35.0	41.0
17	38.573529189631536	40.0	38.0	41.0	34.0	41.0
18	38.511110656972185	40.0	38.0	41.0	34.0	41.0
19	38.546098408549106	40.0	38.0	41.0	34.0	41.0
20	38.50366475306344	40.0	38.0	41.0	34.0	41.0
21	38.41999518415284	40.0	37.0	41.0	34.0	41.0
22	38.4208615035217	40.0	37.0	41.0	34.0	41.0
23	38.37050746767296	40.0	37.0	41.0	34.0	41.0
24	38.357181254448214	40.0	37.0	41.0	34.0	41.0
25	38.26569170936806	40.0	37.0	41.0	34.0	41.0
26	38.16793001205739	40.0	37.0	41.0	34.0	41.0
27	38.14546701722243	40.0	37.0	41.0	34.0	41.0
28	38.04906832905208	40.0	37.0	41.0	34.0	41.0
29	38.026260583534956	40.0	37.0	41.0	34.0	41.0
30	37.98787597149943	40.0	36.0	41.0	34.0	41.0
31	37.922001046691506	40.0	36.0	41.0	34.0	41.0
32	37.82582804582341	40.0	36.0	41.0	34.0	41.0
33	37.785836966691576	40.0	36.0	41.0	33.0	41.0
34	37.72881449303435	40.0	36.0	41.0	33.0	41.0
35	37.6814770256546	40.0	36.0	41.0	33.0	41.0
36	37.594892181001214	40.0	36.0	41.0	33.0	41.0
37	37.54431156931754	40.0	36.0	41.0	33.0	41.0
38	37.51397084368844	40.0	35.0	41.0	33.0	41.0
39	37.522665136021025	40.0	35.0	41.0	33.0	41.0
40	37.4170070487298	40.0	35.0	41.0	33.0	41.0
41	37.286218591480214	39.0	35.0	41.0	33.0	41.0
42	37.259462206706466	39.0	35.0	41.0	33.0	41.0
43	37.16711700464969	39.0	35.0	41.0	33.0	41.0
44	37.071167025485785	39.0	35.0	41.0	32.0	41.0
45	37.08346165226207	39.0	35.0	41.0	32.0	41.0
46	37.00514016158856	39.0	35.0	41.0	32.0	41.0
47	36.93413129492088	39.0	35.0	41.0	32.0	41.0
48	36.884101573502534	39.0	35.0	41.0	32.0	41.0
49	36.87666011225722	39.0	35.0	41.0	32.0	41.0
50	36.82642869391915	39.0	35.0	41.0	32.0	41.0
51	35.9431854429913	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	29.0
11	33.0
12	14.0
13	12.0
14	16.0
15	20.0
16	38.0
17	71.0
18	134.0
19	269.0
20	471.0
21	761.0
22	1227.0
23	1737.0
24	2716.0
25	3911.0
26	5357.0
27	6720.0
28	7978.0
29	9866.0
30	12281.0
31	16613.0
32	22225.0
33	33029.0
34	67340.0
35	84137.0
36	78019.0
37	123818.0
38	241628.0
39	404691.0
40	272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.423676375070972	4.58438439345649	54.98888889876148	17.003050332711066
2	19.395691149592476	4.4836247868632215	53.68292355686742	22.43776050667688
3	21.071908061745916	4.617082396301572	52.45737042305707	21.853639118895448
4	20.235798804212713	4.681412162768526	50.59776036451164	24.485028668507113
5	19.32807381218729	5.624056489354046	49.332578672905356	25.71529102555331
6	21.842532460320353	6.202047001602025	52.25425185103572	19.701168687041907
7	83.47622419812146	2.1315899137323613	9.1479771220604	5.244208766085774
8	84.19940095126309	3.0886284698312054	7.346565954448484	5.365404624457218
9	78.35276702406414	5.7873687970422525	9.856404232614304	6.003459946279314
10	37.869973903795014	32.35298560310489	16.029485068652477	13.747555424447622
11	31.79587560897809	24.820627464012205	24.448954241455205	18.934542685554504
12	31.10770704366516	20.423456907497528	28.32242363283697	20.14641241600034
13	26.586319617646616	21.85292829274664	30.03142740110409	21.529324688502655
14	20.28431268886873	24.98838243513045	31.119524528389064	23.607780347611758
15	18.143126622127483	24.795304282460986	35.08584558545863	21.9757235099529
16	21.986385902184992	25.079545888714833	31.664905891060563	21.269162318039612
17	22.54562837475821	23.656294232267776	29.698049937314018	24.100027455659998
18	23.001623349217336	23.7871750969167	30.321266763279787	22.889934790586175
19	24.603470075551932	26.385511230608884	27.474319184042663	21.536699509796517
20	27.566282317044454	26.027876824490804	27.554198272514753	18.85164258594999
21	26.377425583166215	25.78699561331413	28.223352238347115	19.612226565172538
22	23.221446335735628	24.24645764231406	28.45161628538248	24.080479736567828
23	23.129038936390835	25.708715883676852	28.36222989717011	22.8000152827622
24	24.339842427613466	23.166535015740354	28.500574436381505	23.993048120264675
25	23.04933755445595	25.523101405569854	25.520613514049035	25.906947525925162
26	21.51493045898933	27.656823797748636	26.790593282159776	24.03765246110226
27	22.606848276824135	26.240147283178032	28.63723076348948	22.515773676508353
28	19.82911739382701	26.382134806402057	30.076831421359078	23.711916378411853
29	22.698278290214326	25.99979919161296	28.14596104139585	23.155961476776866
30	24.190302376558375	23.95395268208034	27.82022495870544	24.035519982655842
31	23.018416616982883	25.992157810513294	26.464412933126365	24.525012639377458
32	24.2861750533786	28.095403531562013	25.30985356092802	22.30856785413137
33	24.5765475351659	25.08372199233907	26.5378945862592	23.80183588623583
34	21.394889693109693	25.248189392519087	29.392039280252984	23.964881634118235
35	21.782201090940433	26.467256237721585	26.698008176277778	25.052534495060204
36	22.065643017776875	27.695119556515568	26.693654366116338	23.54558305959122
37	22.583391013913534	27.110553902391132	27.957503258693627	22.34855182500171
38	22.505822110425065	25.953773198477766	27.50426273556112	24.036141955536046
39	22.737107168592857	23.42225472277336	27.792058472559	26.04857963607478
40	22.735507809758044	23.53829709156596	28.203537959449143	25.52265713922685
41	19.308437239826524	26.127392485323664	27.827333220193506	26.73683705465631
42	21.151342883875	25.52443420459887	28.31140582753047	25.01281708399566
43	22.284133205266155	23.732619189995834	29.6375408613969	24.345706743341115
44	22.122509109681364	23.259386681428158	28.324467258014785	26.293636950875694
45	22.510442480392303	22.160804868448295	26.570059469492673	28.75869318166673
46	21.487830212066097	24.394842600877336	27.749408903630634	26.367918283425933
47	19.348421210696866	24.735150619618267	30.704313204217687	25.212114965467176
48	20.50875604535426	23.308700245501583	30.597333868822364	25.58520984032179
49	22.15689532462986	21.72720091767656	31.12689934968293	24.989004408010658
50	20.835114100924873	22.325538828434112	28.917118559581894	27.92222851105912
51	19.023929073766872	22.462461715347892	27.222775580633897	31.290833630251342
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	195.0
1	673.0
2	1151.0
3	2189.5
4	3228.0
5	2282.5
6	1337.0
7	1404.0
8	1471.0
9	1549.0
10	1627.0
11	1632.0
12	1637.0
13	1588.5
14	1540.0
15	1500.0
16	1460.0
17	1630.0
18	1800.0
19	2164.0
20	2528.0
21	2564.0
22	2600.0
23	3317.5
24	4035.0
25	4551.0
26	6942.0
27	8817.0
28	10187.5
29	11558.0
30	13453.5
31	15349.0
32	18325.5
33	21302.0
34	23925.0
35	26548.0
36	30195.0
37	33842.0
38	37243.5
39	40645.0
40	44920.5
41	49196.0
42	54422.5
43	59649.0
44	65705.5
45	71762.0
46	84813.0
47	97864.0
48	111262.0
49	124660.0
50	123988.0
51	123316.0
52	106054.0
53	88792.0
54	78197.0
55	67602.0
56	62670.0
57	57738.0
58	55707.5
59	53677.0
60	50878.0
61	48079.0
62	41247.5
63	34416.0
64	28234.5
65	22053.0
66	17941.0
67	13829.0
68	11623.5
69	9418.0
70	8019.0
71	6620.0
72	5262.0
73	3904.0
74	3272.5
75	1951.5
76	1262.0
77	963.5
78	665.0
79	502.0
80	339.0
81	219.0
82	99.0
83	97.0
84	95.0
85	59.5
86	24.0
87	15.5
88	7.0
89	6.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1125451.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.89204677606445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.13039334288194	19.684265113342153
2	12.9211534200141	7.466371380235051
3	5.211900254874567	4.517473978680546
4	2.6274858878220995	3.036537806976213
5	1.5430349222504107	2.229071857537992
6	1.001606404524259	1.7363075454432373
7	0.7071972539274038	1.4302663298262415
8	0.541530232476011	1.2516733445879973
9	0.4308823391588024	1.1204165428160568
>10	6.5052457660826475	45.3711082729179
>50	0.30191719400996925	5.401167975534442
>100	0.07113013149186484	3.584121727219521
>500	0.003727343134789651	0.6802454477349604
>1k	0.002174283495293963	1.4629764233476683
>5k	6.212238557982751E-4	1.0279962537999963
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	6021	0.5349855302452083	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGCT	5434	0.48282866157655907	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	4677	0.41556673724577975	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	4382	0.3893550230085539	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTG	1904	0.1691766234158573	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1572	0.13967733824040318	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTG	1315	0.11684204821000649	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTG	1227	0.10902296057313914	No Hit
TGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTG	1225	0.10884525403593759	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.885326860076538E-5	0.0	0.0	0.5053973918011535	0.0
2	8.885326860076538E-5	0.0	0.0	1.944731489864952	0.0
3	8.885326860076538E-5	0.0	0.0	2.7811073072039565	0.0
4	8.885326860076538E-5	0.0	0.0	3.5756332350320004	0.0
5	8.885326860076538E-5	0.0	0.0	4.875467701392597	0.0
6	8.885326860076538E-5	0.0	0.0	5.679678635498124	0.0
7	8.885326860076538E-5	0.0	0.0	6.426668064624759	0.0
8	8.885326860076538E-5	0.0	0.0	7.6240547122886735	0.0
9	8.885326860076538E-5	0.0	0.0	8.177610575671443	0.0
10	8.885326860076538E-5	0.0	0.0	9.254423337844118	0.0
11	8.885326860076538E-5	0.0	0.0	11.224122596185884	0.0
12	8.885326860076538E-5	0.0	0.0	13.023223578814182	0.0
13	8.885326860076538E-5	0.0	0.0	13.636844251771068	0.0
14	8.885326860076538E-5	0.0	0.0	13.862087287674008	0.0
15	8.885326860076538E-5	0.0	0.0	14.214745910750446	0.0
16	8.885326860076538E-5	0.0	0.0	14.960224834310868	0.0
17	8.885326860076538E-5	0.0	0.0	15.948095474614178	0.0
18	8.885326860076538E-5	0.0	0.0	17.042145770895402	0.0
19	8.885326860076538E-5	0.0	0.0	17.841292068690684	0.0
20	8.885326860076538E-5	0.0	0.0	18.719695482077853	0.0
21	8.885326860076538E-5	0.0	0.0	19.93360883770151	0.0
22	8.885326860076538E-5	0.0	0.0	21.17577753274021	0.0
23	8.885326860076538E-5	0.0	0.0	22.352283662282943	0.0
24	8.885326860076538E-5	0.0	0.0	23.211939035995346	0.0
25	8.885326860076538E-5	0.0	0.0	23.97714338518514	0.0
26	8.885326860076538E-5	0.0	0.0	24.68983545263188	0.0
27	8.885326860076538E-5	0.0	0.0	25.36112189691066	0.0
28	8.885326860076538E-5	0.0	0.0	26.0738139643574	0.0
29	8.885326860076538E-5	0.0	0.0	26.863630668949604	0.0
30	8.885326860076538E-5	0.0	0.0	27.704004883375642	0.0
31	8.885326860076538E-5	0.0	0.0	28.491422549715626	0.0
32	1.7770653720153076E-4	0.0	0.0	29.23361390233782	0.0
33	1.7770653720153076E-4	0.0	0.0	29.937864909267486	0.0
34	1.7770653720153076E-4	0.0	0.0	30.669660429463388	0.0
35	1.7770653720153076E-4	0.0	0.0	31.408031091535747	0.0
36	1.7770653720153076E-4	0.0	0.0	32.13254064370639	0.0
37	1.7770653720153076E-4	0.0	0.0	32.89374659580915	0.0
38	1.7770653720153076E-4	0.0	0.0	33.665614940143996	0.0
39	1.7770653720153076E-4	0.0	0.0	34.62487482795786	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGATCG	25	3.8907536E-5	45.0	27
ACGGGTA	25	3.8907536E-5	45.0	5
TAGCCGT	20	7.033225E-4	45.0	44
ACGATCC	20	7.033225E-4	45.0	35
CGGATTA	20	7.033225E-4	45.0	6
TTCGGTA	20	7.033225E-4	45.0	35
ACCGTAA	25	3.8907536E-5	45.0	20
TATCGCG	30	2.1654614E-6	44.999996	32
TATTGCG	70	0.0	41.785713	1
CTACGAA	110	0.0	40.909092	11
CGTTTTT	960	0.0	40.07812	1
TTGGGAC	2540	0.0	39.419292	5
TTTCGCG	400	0.0	39.375	1
TACGGGA	200	0.0	39.375	4
TTTTGCG	1140	0.0	39.276318	1
TTTCGTG	370	0.0	38.91892	1
AGGCACG	105	0.0	38.57143	10
CGTTTGG	660	0.0	38.522728	2
TTTGGGA	7155	0.0	38.396225	4
TGGGCGA	1235	0.0	38.25911	6
>>END_MODULE