FastQCFastQC Report
Sat 14 Jan 2017
SRR2933941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932233
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA192720.9973952416711649No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT166800.8632499289681939No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC128790.6665345224928877No Hit
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT113790.5889041331971868No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATG104200.5392724376408021No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTA96750.5007160109572707No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCGTG96060.4971450130496684No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC87170.45113606899374975No Hit
TCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC82940.42924429921236207No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC58820.30441463322487505No Hit
GCTCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC56860.2942709290235701No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC47660.24665762358887358No Hit
GCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG40870.21151693403435298No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39550.20468545977633132No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG34450.178291127415793No Hit
CGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG33230.17197718908640935No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCGTGATCAG31390.16245452799947005No Hit
TCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG29770.15407044595553435No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG28200.14594513187591765No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC24800.12834891030222548No Hit
GCTCAACTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGT24070.12457089802316802No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAG207.034481E-445.01
CCGGTAT207.034481E-445.042
CGTCATA253.891797E-545.038
ACTTGAC95150.043.581196
TTGACCC89300.043.563838
GCTCAAC105800.043.511341
ACCCTGC79350.043.41209811
GCATTAG75300.043.3565716
CTCAACT100650.043.32342
CTTGACC102250.043.3056267
TCAACTT98300.043.2375343
TGCATTA75650.042.97752815
AACTTGA96100.042.9396485
CAACTTG97150.042.7997934
CCTGCAT79750.042.77115613
CATTAGA73600.042.76834517
CCCTGCA80800.042.57735412
TGACCCT80500.042.456529
ATTAGAA74050.042.386918
CTGCATT79350.041.2003814
Produced by FastQC (version 0.11.5)