##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933941.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1932233
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30543624914801	33.0	31.0	34.0	31.0	34.0
2	32.54385884104039	34.0	31.0	34.0	31.0	34.0
3	32.6333408031019	34.0	31.0	34.0	31.0	34.0
4	36.03030483383733	37.0	35.0	37.0	35.0	37.0
5	36.01742232950167	37.0	35.0	37.0	35.0	37.0
6	35.4681930181298	37.0	35.0	37.0	33.0	37.0
7	35.96200044197568	37.0	35.0	37.0	35.0	37.0
8	36.010553592656784	37.0	35.0	37.0	35.0	37.0
9	37.90826572157706	39.0	38.0	39.0	35.0	39.0
10	37.569221724295154	39.0	37.0	39.0	35.0	39.0
11	37.47373634546144	39.0	37.0	39.0	35.0	39.0
12	37.288123637263205	39.0	37.0	39.0	35.0	39.0
13	37.160514803338934	39.0	37.0	39.0	34.0	39.0
14	38.082504542671614	40.0	38.0	41.0	33.0	41.0
15	38.23378339982808	40.0	38.0	41.0	33.0	41.0
16	38.37241730164013	40.0	38.0	41.0	34.0	41.0
17	38.402842203812895	40.0	38.0	41.0	34.0	41.0
18	38.34475604132628	40.0	37.0	41.0	34.0	41.0
19	38.38729852973218	40.0	37.0	41.0	34.0	41.0
20	38.31795596079769	40.0	37.0	41.0	34.0	41.0
21	38.22555871885016	40.0	37.0	41.0	34.0	41.0
22	38.29251596468956	40.0	37.0	41.0	34.0	41.0
23	38.232453332491474	40.0	37.0	41.0	34.0	41.0
24	38.20220439253444	40.0	37.0	41.0	34.0	41.0
25	38.12050772344743	40.0	37.0	41.0	34.0	41.0
26	38.06272638962278	40.0	37.0	41.0	34.0	41.0
27	38.05347129461095	40.0	36.0	41.0	34.0	41.0
28	37.95844548768187	40.0	36.0	41.0	34.0	41.0
29	37.937779760515426	40.0	36.0	41.0	34.0	41.0
30	37.863358611513206	40.0	36.0	41.0	34.0	41.0
31	37.79765121494147	40.0	36.0	41.0	33.0	41.0
32	37.69878477388597	40.0	36.0	41.0	33.0	41.0
33	37.66345621878935	40.0	36.0	41.0	33.0	41.0
34	37.59793099486449	40.0	36.0	41.0	33.0	41.0
35	37.511057413883314	40.0	36.0	41.0	33.0	41.0
36	37.39435927240659	39.0	35.0	41.0	33.0	41.0
37	37.28678684195953	39.0	35.0	41.0	32.0	41.0
38	37.246894137508264	39.0	35.0	41.0	32.0	41.0
39	37.25102510929065	39.0	35.0	41.0	32.0	41.0
40	37.16806927528926	39.0	35.0	41.0	32.0	41.0
41	37.058762581945345	39.0	35.0	41.0	32.0	41.0
42	37.047474088269894	39.0	35.0	41.0	32.0	41.0
43	36.92618229789058	39.0	35.0	41.0	32.0	41.0
44	36.812384427757934	39.0	35.0	41.0	31.0	41.0
45	36.824091607999655	39.0	35.0	41.0	32.0	41.0
46	36.793925991327136	39.0	35.0	41.0	32.0	41.0
47	36.70856723800908	39.0	35.0	40.0	31.0	41.0
48	36.664182839233156	39.0	35.0	40.0	31.0	41.0
49	36.67048797945176	39.0	35.0	40.0	31.0	41.0
50	36.57783145200398	39.0	35.0	40.0	31.0	41.0
51	35.59182355337063	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	24.0
10	42.0
11	42.0
12	28.0
13	17.0
14	26.0
15	59.0
16	90.0
17	145.0
18	290.0
19	497.0
20	904.0
21	1534.0
22	2188.0
23	3368.0
24	4910.0
25	7093.0
26	10007.0
27	12652.0
28	15350.0
29	18856.0
30	24292.0
31	31963.0
32	43585.0
33	66744.0
34	131640.0
35	133483.0
36	136934.0
37	224665.0
38	433868.0
39	626608.0
40	320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.15923338437963	4.313558458012051	52.3649063027078	15.162301854900523
2	18.49523323532928	3.9444000801145616	50.82451236470964	26.73585431984652
3	19.31785659389939	3.7556029733474174	56.12464956348432	20.80189086926887
4	18.25628689707711	4.201408422276195	48.80798537236451	28.73431930828218
5	17.49012670832141	10.756984276740953	48.0106695207048	23.742219494232838
6	20.705370418577886	10.9180414577331	50.073153703512986	18.303434420176036
7	77.84977277585054	1.8736870760410367	9.30943628434045	10.967103863767983
8	78.37760766946843	2.3605331241108085	13.418102268204715	5.843756938216043
9	73.3739150506176	4.729243315894097	15.7766687557867	6.120172877701602
10	42.257377862814685	28.862512957805812	16.196545654690713	12.683563524688793
11	27.54755767032237	27.1689283849308	26.577333064904696	18.70618087984213
12	26.103114893493697	19.008939398095364	29.7444976873907	25.143448021020237
13	25.119434353931435	19.920268414834027	30.76456100273621	24.195736228498323
14	19.567050143538587	21.965104622475653	32.84039761250325	25.627447621482503
15	17.932775188085497	21.5537153128013	38.99783307706679	21.515676422046408
16	26.073408331189874	22.2917215470391	30.26327570225744	21.37159441951359
17	23.327362693836612	22.322359674014468	27.28242401408112	27.0678536180678
18	23.002350130651944	25.593549018156715	30.432354690143477	20.971746161047868
19	22.337368215944974	24.03198786067726	32.14513984597095	21.485504077406816
20	22.20570707569946	27.503515362795273	32.39966401567513	17.891113545830134
21	22.109393639379928	29.02626132562688	30.636005078062535	18.228339956930657
22	24.25778878634202	23.143378671205802	30.689880568233747	21.90895197421843
23	20.45923033091765	26.487126552543096	30.728126473360096	22.32551664317916
24	21.493836405857884	28.51576388561835	27.331382912930273	22.65901679559349
25	21.515417654082093	27.30592014524128	24.985030273264144	26.19363192741248
26	19.934241884907255	24.620322704352944	30.36621359846354	25.079221812276266
27	20.331761231694106	22.215850779900766	33.94321492283798	23.509173065567143
28	21.065937700059983	23.912074786011832	29.979148477435174	25.04283903649301
29	19.998261079279775	27.06500717046029	29.193787705727004	23.742944044532933
30	22.116794403159453	25.384412749394098	30.94647488165247	21.55231796579398
31	24.451502484431227	24.43307820537171	28.487609931100444	22.627809379096618
32	23.752363198434143	28.594791621921374	25.092833007199445	22.56001217244504
33	23.582611413840876	23.348633420503635	27.597499887435934	25.47125527821955
34	21.645370925763093	23.035265415713322	30.540209177671635	24.779154480851947
35	23.43821888975087	26.741133186318628	27.105633740858377	22.715014183072125
36	24.523077703361864	24.507810393467043	29.522267759633543	21.446844143537554
37	22.044649894707316	27.92390979762793	28.084915225027206	21.94652508263755
38	21.728487195902357	29.193839459319864	26.25734060022782	22.820332744549958
39	22.380478958800516	24.907296376782718	28.405166457668408	24.30705820674836
40	21.739510711182348	22.360916100698	32.17448413312473	23.72508905499492
41	20.969417249368995	25.282613432231	26.820419690585968	26.927549627814034
42	22.534187129606007	22.221802443080104	28.133046066390545	27.110964360923344
43	23.287201905774303	22.4578505801319	29.628983668118703	24.625963845975097
44	23.2601865302994	21.36031213626928	29.340302127124417	26.0391992063069
45	22.389846359108866	21.855387005604396	28.074875028011633	27.679891607275103
46	22.78151754990211	21.154643358228537	30.962311481068795	25.101527610800563
47	19.885231232465237	22.571449716467942	33.799753963419526	23.743565087647298
48	19.11648336406634	24.288219898945933	31.559547942717053	25.035748794270667
49	23.790557349967628	21.963552014689743	30.77641257550202	23.46947805984061
50	20.58861431307715	21.29836308561131	31.545781487015283	26.56724111429626
51	20.459747866846286	21.925099095191936	26.94773352903092	30.66741950893086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	344.0
1	1176.0
2	2008.0
3	4324.5
4	6641.0
5	4397.0
6	2153.0
7	2310.0
8	2467.0
9	2627.5
10	2788.0
11	2817.5
12	2847.0
13	2947.0
14	3047.0
15	2911.0
16	2775.0
17	2867.0
18	2959.0
19	2934.0
20	2909.0
21	3475.0
22	4041.0
23	5119.0
24	6197.0
25	7680.5
26	12067.0
27	14970.0
28	16705.0
29	18440.0
30	30897.5
31	43355.0
32	39360.0
33	35365.0
34	39172.5
35	42980.0
36	46219.5
37	49459.0
38	63714.5
39	77970.0
40	82105.0
41	86240.0
42	90787.5
43	95335.0
44	109355.5
45	123376.0
46	157687.0
47	191998.0
48	223434.0
49	254870.0
50	248773.5
51	242677.0
52	199402.5
53	156128.0
54	135072.5
55	114017.0
56	99944.0
57	85871.0
58	76112.5
59	66354.0
60	58995.0
61	51636.0
62	48130.5
63	44625.0
64	38730.0
65	32835.0
66	27926.0
67	23017.0
68	17867.0
69	12717.0
70	10077.0
71	7437.0
72	6100.5
73	4764.0
74	3689.0
75	1990.0
76	1366.0
77	1082.5
78	799.0
79	568.5
80	338.0
81	282.5
82	227.0
83	135.5
84	44.0
85	40.0
86	36.0
87	31.0
88	26.0
89	15.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1932233.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.843777449088606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.96023460689679	17.437780808281218
2	13.798649581755424	7.408157569412044
3	6.024082081487914	4.8512735619151215
4	3.2221068156890693	3.4597407311019577
5	1.9223030506377299	2.580093764051167
6	1.282811946432254	2.0661311039435764
7	0.9281030537112804	1.74396542765516
8	0.6635419375246351	1.424957767923869
9	0.555681696963387	1.342493622523535
>10	6.233236435678469	37.63864028156178
>50	0.30418752924054726	5.190319506821147
>100	0.09163684021755922	4.685195406744129
>500	0.006614933449379569	1.2504047600169481
>1k	0.0046693647877973435	2.732538110803055
>5k	0.0011673411969493359	2.4995560861419737
>10k+	9.727843307911131E-4	3.6887514911033765
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	19272	0.9973952416711649	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	16680	0.8632499289681939	No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	12879	0.6665345224928877	No Hit
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT	11379	0.5889041331971868	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATG	10420	0.5392724376408021	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTA	9675	0.5007160109572707	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCGTG	9606	0.4971450130496684	No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	8717	0.45113606899374975	No Hit
TCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	8294	0.42924429921236207	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC	5882	0.30441463322487505	No Hit
GCTCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	5686	0.2942709290235701	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	4766	0.24665762358887358	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	4087	0.21151693403435298	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3955	0.20468545977633132	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG	3445	0.178291127415793	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	3323	0.17197718908640935	No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCGTGATCAG	3139	0.16245452799947005	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	2977	0.15407044595553435	No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	2820	0.14594513187591765	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC	2480	0.12834891030222548	No Hit
GCTCAACTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGT	2407	0.12457089802316802	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0701437145520234E-4	0.0	0.0	0.6151432047791338	0.0
2	2.0701437145520234E-4	0.0	0.0	2.2777273755287277	0.0
3	2.0701437145520234E-4	0.0	0.0	3.2756401531285304	0.0
4	2.0701437145520234E-4	0.0	0.0	4.222420380978899	0.0
5	2.0701437145520234E-4	0.0	0.0	5.989805577277688	0.0
6	2.5876796431900293E-4	0.0	0.0	6.867546512247746	0.0
7	2.5876796431900293E-4	0.0	0.0	7.731417484330306	0.0
8	2.5876796431900293E-4	0.0	0.0	9.392138525736803	0.0
9	2.5876796431900293E-4	0.0	0.0	10.136510451896847	0.0
10	2.5876796431900293E-4	0.0	0.0	11.540274904734574	0.0
11	2.5876796431900293E-4	0.0	0.0	14.149173520998762	0.0
12	2.5876796431900293E-4	0.0	0.0	16.52497395500439	0.0
13	2.5876796431900293E-4	0.0	0.0	17.89225212487314	0.0
14	2.5876796431900293E-4	0.0	0.0	18.17456797394517	0.0
15	2.5876796431900293E-4	0.0	0.0	19.121658723352724	0.0
16	2.5876796431900293E-4	0.0	0.0	19.957686262474557	0.0
17	2.5876796431900293E-4	0.0	0.0	21.116914989030825	0.0
18	2.5876796431900293E-4	0.0	0.0	22.340939213852575	0.0
19	3.1052155718280355E-4	0.0	0.0	23.35375702619715	0.0
20	3.1052155718280355E-4	0.0	0.0	24.67010966068792	0.0
21	3.1052155718280355E-4	0.0	0.0	26.202274777420737	0.0
22	3.1052155718280355E-4	0.0	0.0	27.78329528581698	0.0
23	3.1052155718280355E-4	0.0	0.0	29.227324033902743	0.0
24	3.1052155718280355E-4	0.0	0.0	30.313062658592415	0.0
25	3.1052155718280355E-4	0.0	0.0	31.788609344732233	0.0
26	3.1052155718280355E-4	0.0	0.0	32.644096234770856	0.0
27	3.622751500466041E-4	0.0	0.0	33.57022677906857	0.0
28	3.622751500466041E-4	0.0	0.0	34.4152594433487	0.0
29	3.622751500466041E-4	0.0	0.0	35.37130356432169	0.0
30	3.622751500466041E-4	0.0	0.0	36.4022351341686	0.0
31	3.622751500466041E-4	0.0	0.0	37.25539311252835	0.0
32	4.140287429104047E-4	0.0	0.0	38.29569208268361	0.0
33	4.140287429104047E-4	0.0	0.0	39.09797627925825	0.0
34	4.140287429104047E-4	0.0	0.0	40.260568989350666	0.0
35	4.140287429104047E-4	0.0	0.0	41.14860888930062	0.0
36	4.140287429104047E-4	0.0	0.0	41.96398674486979	0.0
37	4.140287429104047E-4	0.0	0.0	42.812021117536034	0.0
38	4.140287429104047E-4	0.0	0.0	43.78519567774694	0.0
39	4.140287429104047E-4	0.0	0.0	45.53343204468612	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTAG	20	7.034481E-4	45.0	1
CCGGTAT	20	7.034481E-4	45.0	42
CGTCATA	25	3.891797E-5	45.0	38
ACTTGAC	9515	0.0	43.58119	6
TTGACCC	8930	0.0	43.56383	8
GCTCAAC	10580	0.0	43.51134	1
ACCCTGC	7935	0.0	43.412098	11
GCATTAG	7530	0.0	43.35657	16
CTCAACT	10065	0.0	43.3234	2
CTTGACC	10225	0.0	43.305626	7
TCAACTT	9830	0.0	43.237534	3
TGCATTA	7565	0.0	42.977528	15
AACTTGA	9610	0.0	42.939648	5
CAACTTG	9715	0.0	42.799793	4
CCTGCAT	7975	0.0	42.771156	13
CATTAGA	7360	0.0	42.768345	17
CCCTGCA	8080	0.0	42.577354	12
TGACCCT	8050	0.0	42.45652	9
ATTAGAA	7405	0.0	42.3869	18
CTGCATT	7935	0.0	41.20038	14
>>END_MODULE