FastQCFastQC Report
Sat 14 Jan 2017
SRR2933940.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933940.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375530
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC23510.6260485180944265Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC22420.5970228743376028Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT19750.5259233616488697Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGC18730.4987617500599153No Hit
TCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC16780.4468351396692674Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCG16570.44124304316565915No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC16340.4351183660426597No Hit
TCCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG6850.18240886214150667Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG5020.13367773546720635Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.1296833808217719No Hit
GTTTGTGGGAGAAAAGGCTCCTGCCAGTGTGAAGACAGACGGACTGCTGTG4810.12808563896359812No Hit
CGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG4480.11929805874364233Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG4250.11317338162064282Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT3820.10172289830373071No Hit
ATGTTGGGGCTTCCCTCTTGATTGGTAGTGTTAAGAACTACCACTTTTATA3770.10039144675525258No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGG207.027213E-445.02
TTCTACG207.027213E-445.01
ATGGGCG253.8857717E-545.012
CGGGTCA207.027213E-445.06
CGTGGCA253.8857717E-545.015
CGGAAAG207.027213E-445.012
GGTACGT453.8380676E-1045.07
GAAGCGT207.027213E-445.040
TACCCCA207.027213E-445.013
ACCGGGT207.027213E-445.04
ACTGCCG207.027213E-445.017
CAAGTTA253.8857717E-545.015
TACTTCG207.027213E-445.024
GCGCGTT207.027213E-445.043
GAGTCGA207.027213E-445.010
GAACTTC253.8857717E-545.09
ATAGCGC207.027213E-445.037
GGTCCAC253.8857717E-545.010
GAATCGC253.8857717E-545.017
AAGCGTG207.027213E-445.041
Produced by FastQC (version 0.11.5)