##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933940.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375530
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39531861635555	33.0	31.0	34.0	31.0	34.0
2	32.611695470401834	34.0	31.0	34.0	31.0	34.0
3	32.68440071365803	34.0	31.0	34.0	31.0	34.0
4	36.075368146353156	37.0	35.0	37.0	35.0	37.0
5	36.116616515325006	37.0	35.0	37.0	35.0	37.0
6	35.68143423960802	37.0	35.0	37.0	35.0	37.0
7	36.04625196389103	37.0	35.0	37.0	35.0	37.0
8	36.01121881074748	37.0	35.0	37.0	35.0	37.0
9	37.86554735973158	39.0	38.0	39.0	35.0	39.0
10	37.58070194125636	39.0	37.0	39.0	35.0	39.0
11	37.56985061113626	39.0	37.0	39.0	35.0	39.0
12	37.321516257023404	39.0	37.0	39.0	35.0	39.0
13	37.14808404122174	39.0	37.0	39.0	33.0	39.0
14	38.346140121960964	40.0	38.0	41.0	34.0	41.0
15	38.41011903176843	40.0	38.0	41.0	34.0	41.0
16	38.5247570100924	40.0	38.0	41.0	35.0	41.0
17	38.49342529225361	40.0	38.0	41.0	34.0	41.0
18	38.45874097941576	40.0	37.0	41.0	34.0	41.0
19	38.50014379676723	40.0	37.0	41.0	34.0	41.0
20	38.44900274279019	40.0	37.0	41.0	34.0	41.0
21	38.375506617314194	40.0	37.0	41.0	34.0	41.0
22	38.36604798551381	40.0	37.0	41.0	34.0	41.0
23	38.290908848826994	40.0	37.0	41.0	34.0	41.0
24	38.26210955183341	40.0	37.0	41.0	34.0	41.0
25	38.16346230660666	40.0	37.0	41.0	34.0	41.0
26	38.0771656059436	40.0	37.0	41.0	34.0	41.0
27	38.0700742949964	40.0	36.0	41.0	34.0	41.0
28	38.00919500439379	40.0	36.0	41.0	34.0	41.0
29	38.02324181823023	40.0	36.0	41.0	34.0	41.0
30	37.96545415812319	40.0	36.0	41.0	34.0	41.0
31	37.85222485553751	40.0	36.0	41.0	34.0	41.0
32	37.76906505472266	40.0	36.0	41.0	34.0	41.0
33	37.692320187468376	40.0	36.0	41.0	33.0	41.0
34	37.61684818789444	40.0	36.0	41.0	33.0	41.0
35	37.60828961734082	40.0	36.0	41.0	33.0	41.0
36	37.51175938007616	40.0	35.0	41.0	33.0	41.0
37	37.44421750592496	40.0	35.0	41.0	33.0	41.0
38	37.418368705562806	40.0	35.0	41.0	33.0	41.0
39	37.39412030996192	39.0	35.0	41.0	33.0	41.0
40	37.272508188427025	39.0	35.0	41.0	33.0	41.0
41	37.13399728383884	39.0	35.0	41.0	32.0	41.0
42	37.13724602561713	39.0	35.0	41.0	32.0	41.0
43	37.001366069288736	39.0	35.0	41.0	32.0	41.0
44	36.92632546001651	39.0	35.0	41.0	32.0	41.0
45	36.946574175165765	39.0	35.0	41.0	32.0	41.0
46	36.85358293611696	39.0	35.0	41.0	32.0	41.0
47	36.714779112188104	39.0	35.0	41.0	31.0	41.0
48	36.719788032913485	39.0	35.0	41.0	31.0	41.0
49	36.74080632705776	39.0	35.0	41.0	32.0	41.0
50	36.65340984741565	39.0	35.0	41.0	31.0	41.0
51	35.66108965994727	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	9.0
11	7.0
12	4.0
13	5.0
14	2.0
15	8.0
16	16.0
17	17.0
18	36.0
19	79.0
20	196.0
21	308.0
22	440.0
23	622.0
24	890.0
25	1194.0
26	1779.0
27	2299.0
28	2665.0
29	3300.0
30	4219.0
31	5742.0
32	7848.0
33	11258.0
34	22447.0
35	33011.0
36	26642.0
37	42914.0
38	80656.0
39	126832.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.720102255478924	4.732245093601044	52.487417782866885	16.060234868053154
2	18.80488909008601	6.750991931403616	52.09836764040156	22.34575133810881
3	21.245972359065853	6.9038425691689085	49.98349000079887	21.86669507096637
4	19.428008414773785	5.240593294810002	50.22980853726733	25.101589753148883
5	19.967246291907436	5.688493595718052	48.13756557398876	26.20669453838575
6	21.507469443186963	8.130908316246371	51.425984608420094	18.935637632146566
7	80.78449125236332	2.2293824727718157	11.72156685218225	5.264559422682608
8	80.59222964876308	4.508028652837323	8.505578781988124	6.394162916411473
9	74.48619284744228	5.718318110403962	10.90858253668149	8.886906505472265
10	35.61153569621601	32.50898729795223	17.711501078475756	14.167975927356002
11	31.22653316645807	23.33501983862807	26.371528240087343	19.06691875482651
12	28.613692647724548	21.179399781641948	30.667057225787552	19.539850344845952
13	23.699837562911085	24.812664767129124	32.601390035416614	18.88610763454318
14	19.780576784810798	28.506910233536605	29.22855697281176	22.48395600884084
15	16.14731179932362	24.564482198492797	37.66197108087237	21.626234921311212
16	18.77559715601949	24.959923308390806	33.06713178707427	23.197347748515433
17	19.930764519479137	23.320640161904507	30.532580619391258	26.21601469922509
18	20.29744627593002	23.765611269405905	32.133517961281385	23.803424493382686
19	22.02380635368679	25.173221846457004	29.867121135461883	22.935850664394323
20	25.170825233669746	25.033952014486193	30.28999014725854	19.50523260458552
21	24.017521902377972	27.21087529624797	28.549782973397598	20.221819827976457
22	21.17380768513834	24.575133810880622	28.80755199318297	25.44350651079807
23	21.00444704817192	26.379250659068514	28.732724416158494	23.88357787660107
24	22.77687534950603	22.60937874470748	29.469017122466912	25.144728783319575
25	19.780576784810798	27.064415625915373	27.607914148004152	25.547093441269674
26	19.452773413575482	28.74284344792693	27.236172875669055	24.568210262828536
27	20.949058663755228	29.268500519266105	27.608180438313852	22.17426037866482
28	17.068676270870505	28.078182834926636	30.99299656485501	23.860144329347854
29	19.86232790988736	25.509546507602586	28.830186669507096	25.797938913002955
30	23.19148936170213	26.497749846883075	27.695523659894018	22.615237131520786
31	23.120389849013396	29.8719143610364	24.428674140548026	22.57902164940218
32	24.93089766463398	29.439725188400395	22.818150347508855	22.81122679945677
33	24.95859185684233	28.416371528240088	23.30785822703912	23.317178387878464
34	21.45767315527388	29.313503581604667	25.96224003408516	23.266583229036293
35	21.72343088435012	27.273187228716743	25.18706894256118	25.816312944371955
36	24.181024152531087	30.59782174526669	24.224163182701783	20.996990919500437
37	22.968071791867494	29.283945357228454	26.637019678853886	21.11096317205017
38	21.145847202620295	29.33214390328336	24.86219476473251	24.659814129363834
39	22.0168828056347	26.715042739594708	27.137911751391368	24.130162703379224
40	25.682901499214445	25.660533113200014	27.01754853140894	21.639016856176603
41	21.34183687055628	28.90075360157644	24.528533006683887	25.228876521183395
42	23.713950949324953	25.748675205709265	25.57265731100045	24.96471653396533
43	22.91694405240593	26.36487098234495	26.013634063856415	24.7045509013927
44	21.334647032194496	25.635501824088617	26.839932894841954	26.18991824887492
45	21.687215402231512	24.726120416478047	25.32074667802839	28.265917503262056
46	24.164247863020265	27.406066093254864	26.339839693233564	22.089846350491303
47	19.858866135861316	26.77336031741805	30.171490959443986	23.19628258727665
48	20.64096077543738	25.608606502809362	28.71621441695737	25.034218304795893
49	22.042180385055786	23.35046467659042	30.93095092269592	23.67640401565787
50	22.551061166884136	23.312385162303944	27.749314302452532	26.38723936835938
51	20.25057918142359	24.90187202087716	24.91651798791042	29.931030809788833
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	193.5
2	335.0
3	682.5
4	1030.0
5	710.0
6	390.0
7	414.5
8	439.0
9	517.5
10	596.0
11	602.5
12	609.0
13	585.0
14	561.0
15	548.0
16	535.0
17	516.0
18	497.0
19	509.0
20	521.0
21	737.0
22	953.0
23	1383.5
24	1814.0
25	1673.5
26	2061.5
27	2590.0
28	3372.5
29	4155.0
30	4763.0
31	5371.0
32	6246.5
33	7122.0
34	8242.0
35	9362.0
36	10367.5
37	11373.0
38	12586.5
39	13800.0
40	15821.5
41	17843.0
42	20752.0
43	23661.0
44	26400.0
45	29139.0
46	36272.0
47	43405.0
48	44615.0
49	45825.0
50	44033.0
51	42241.0
52	35019.5
53	27798.0
54	23244.5
55	18691.0
56	16711.0
57	14731.0
58	14140.0
59	13549.0
60	12522.5
61	11496.0
62	9774.5
63	8053.0
64	6659.5
65	5266.0
66	4464.0
67	3662.0
68	3088.0
69	2514.0
70	1945.5
71	1377.0
72	1267.0
73	1157.0
74	950.0
75	583.0
76	423.0
77	285.5
78	148.0
79	101.5
80	55.0
81	54.5
82	54.0
83	50.0
84	46.0
85	27.0
86	8.0
87	6.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	375530.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.618830839846947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.42778309134057	18.07038873425934
2	14.351216266335207	7.927276288119439
3	5.8677506793767025	4.861812402723064
4	3.0339204087203058	3.3517333820002175
5	1.7998103343711662	2.48543285844028
6	1.1160863296733152	1.849499972514777
7	0.7603260701494375	1.4699521980208043
8	0.5500646712455142	1.2153714484884703
9	0.4364642569778001	1.0849169232998415
>10	6.1742749429505865	43.00944072537514
>50	0.3995939720921681	6.728301096384874
>100	0.07374053350329286	3.928488970008496
>500	0.001992987391980888	0.32668603106347793
>1k	0.006975455871933109	3.6906989693018026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	2351	0.6260485180944265	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	2242	0.5970228743376028	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	1975	0.5259233616488697	Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGC	1873	0.4987617500599153	No Hit
TCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1678	0.4468351396692674	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCG	1657	0.44124304316565915	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	1634	0.4351183660426597	No Hit
TCCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG	685	0.18240886214150667	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG	502	0.13367773546720635	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	487	0.1296833808217719	No Hit
GTTTGTGGGAGAAAAGGCTCCTGCCAGTGTGAAGACAGACGGACTGCTGTG	481	0.12808563896359812	No Hit
CGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG	448	0.11929805874364233	Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG	425	0.11317338162064282	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT	382	0.10172289830373071	No Hit
ATGTTGGGGCTTCCCTCTTGATTGGTAGTGTTAAGAACTACCACTTTTATA	377	0.10039144675525258	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6629030969563017E-4	0.0	0.0	0.6050115836284717	0.0
2	2.6629030969563017E-4	0.0	0.0	2.6072484222299153	0.0
3	2.6629030969563017E-4	0.0	0.0	3.618086437834527	0.0
4	2.6629030969563017E-4	0.0	0.0	4.691768966527308	0.0
5	2.6629030969563017E-4	0.0	0.0	6.806912896439699	0.0
6	2.6629030969563017E-4	0.0	0.0	7.77514446249301	0.0
7	2.6629030969563017E-4	0.0	0.0	8.757755705269885	0.0
8	2.6629030969563017E-4	0.0	0.0	10.263094825979282	0.0
9	2.6629030969563017E-4	0.0	0.0	10.8958006018161	0.0
10	2.6629030969563017E-4	0.0	0.0	12.637339227225521	0.0
11	2.6629030969563017E-4	0.0	0.0	14.839560088408383	0.0
12	2.6629030969563017E-4	0.0	0.0	17.53681463531542	0.0
13	2.6629030969563017E-4	0.0	0.0	18.29068250206375	0.0
14	2.6629030969563017E-4	0.0	0.0	18.556706521449684	0.0
15	2.6629030969563017E-4	0.0	0.0	19.07304343194951	0.0
16	2.6629030969563017E-4	0.0	0.0	20.030623385614998	0.0
17	2.6629030969563017E-4	0.0	0.0	21.346363805821106	0.0
18	2.6629030969563017E-4	0.0	0.0	22.92812824541315	0.0
19	2.6629030969563017E-4	0.0	0.0	23.84283545921764	0.0
20	2.6629030969563017E-4	0.0	0.0	24.836897185311425	0.0
21	2.6629030969563017E-4	0.0	0.0	26.131866961361276	0.0
22	2.6629030969563017E-4	0.0	0.0	27.463851090458817	0.0
23	2.6629030969563017E-4	0.0	0.0	28.557505392378772	0.0
24	2.6629030969563017E-4	0.0	0.0	29.457300348840306	0.0
25	2.6629030969563017E-4	0.0	0.0	30.196522248555375	0.0
26	5.325806193912603E-4	0.0	0.0	30.899794956461534	0.0
27	5.325806193912603E-4	0.0	0.0	31.58495992330839	0.0
28	5.325806193912603E-4	0.0	0.0	32.287700050595156	0.0
29	5.325806193912603E-4	0.0	0.0	32.97819082363593	0.0
30	5.325806193912603E-4	0.0	0.0	33.79277288099486	0.0
31	5.325806193912603E-4	0.0	0.0	34.55409687641467	0.0
32	5.325806193912603E-4	0.0	0.0	35.304502969136955	0.0
33	5.325806193912603E-4	0.0	0.0	36.00138470961042	0.0
34	5.325806193912603E-4	0.0	0.0	36.65965435517801	0.0
35	5.325806193912603E-4	0.0	0.0	37.38369770724044	0.0
36	5.325806193912603E-4	0.0	0.0	38.08164460895268	0.0
37	5.325806193912603E-4	0.0	0.0	38.781455542832795	0.0
38	5.325806193912603E-4	0.0	0.0	39.45383857481426	0.0
39	5.325806193912603E-4	0.0	0.0	40.17335499161185	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGG	20	7.027213E-4	45.0	2
TTCTACG	20	7.027213E-4	45.0	1
ATGGGCG	25	3.8857717E-5	45.0	12
CGGGTCA	20	7.027213E-4	45.0	6
CGTGGCA	25	3.8857717E-5	45.0	15
CGGAAAG	20	7.027213E-4	45.0	12
GGTACGT	45	3.8380676E-10	45.0	7
GAAGCGT	20	7.027213E-4	45.0	40
TACCCCA	20	7.027213E-4	45.0	13
ACCGGGT	20	7.027213E-4	45.0	4
ACTGCCG	20	7.027213E-4	45.0	17
CAAGTTA	25	3.8857717E-5	45.0	15
TACTTCG	20	7.027213E-4	45.0	24
GCGCGTT	20	7.027213E-4	45.0	43
GAGTCGA	20	7.027213E-4	45.0	10
GAACTTC	25	3.8857717E-5	45.0	9
ATAGCGC	20	7.027213E-4	45.0	37
GGTCCAC	25	3.8857717E-5	45.0	10
GAATCGC	25	3.8857717E-5	45.0	17
AAGCGTG	20	7.027213E-4	45.0	41
>>END_MODULE