##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933938.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351290
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31384326340061	33.0	31.0	34.0	31.0	34.0
2	32.541128412422786	34.0	31.0	34.0	31.0	34.0
3	32.6003928378263	34.0	31.0	34.0	31.0	34.0
4	36.01579891257935	37.0	35.0	37.0	35.0	37.0
5	36.06502604685588	37.0	35.0	37.0	35.0	37.0
6	35.748879842864866	37.0	35.0	37.0	35.0	37.0
7	36.07852771214666	37.0	35.0	37.0	35.0	37.0
8	36.07619630504711	37.0	36.0	37.0	35.0	37.0
9	37.88401036180933	39.0	38.0	39.0	35.0	39.0
10	37.506923055025766	39.0	37.0	39.0	35.0	39.0
11	37.5625209940505	39.0	37.0	39.0	35.0	39.0
12	37.34451592701187	39.0	37.0	39.0	35.0	39.0
13	37.16681943693245	39.0	37.0	39.0	34.0	39.0
14	38.34930399385124	40.0	38.0	41.0	34.0	41.0
15	38.40973554612998	40.0	38.0	41.0	34.0	41.0
16	38.46578894930115	40.0	38.0	41.0	34.0	41.0
17	38.454536138233365	40.0	38.0	41.0	34.0	41.0
18	38.44574568020724	40.0	37.0	41.0	34.0	41.0
19	38.48444305274844	40.0	37.0	41.0	34.0	41.0
20	38.419206353724846	40.0	37.0	41.0	34.0	41.0
21	38.319311679808706	40.0	37.0	41.0	34.0	41.0
22	38.33459534857241	40.0	37.0	41.0	34.0	41.0
23	38.27860172507045	40.0	37.0	41.0	34.0	41.0
24	38.258438896638104	40.0	37.0	41.0	34.0	41.0
25	38.1878448005921	40.0	37.0	41.0	34.0	41.0
26	38.143465512824164	40.0	37.0	41.0	34.0	41.0
27	38.14616129124086	40.0	37.0	41.0	34.0	41.0
28	38.062418514617555	40.0	37.0	41.0	34.0	41.0
29	37.968664066725495	40.0	36.0	41.0	34.0	41.0
30	37.909140596088704	40.0	36.0	41.0	34.0	41.0
31	37.87028096444533	40.0	36.0	41.0	34.0	41.0
32	37.731239147143384	40.0	36.0	41.0	33.0	41.0
33	37.65864670215492	40.0	36.0	41.0	33.0	41.0
34	37.69838025562925	40.0	36.0	41.0	33.0	41.0
35	37.63241196732045	40.0	36.0	41.0	33.0	41.0
36	37.550587833413985	40.0	36.0	41.0	33.0	41.0
37	37.45320390560506	39.0	36.0	41.0	33.0	41.0
38	37.31812462637707	39.0	35.0	41.0	32.0	41.0
39	37.277426058242476	39.0	35.0	41.0	32.0	41.0
40	37.32812491104216	39.0	35.0	41.0	33.0	41.0
41	37.31058384810271	39.0	35.0	41.0	33.0	41.0
42	37.28896353440177	39.0	35.0	41.0	33.0	41.0
43	37.11273306954368	39.0	35.0	41.0	32.0	41.0
44	36.956580033590484	39.0	35.0	41.0	32.0	41.0
45	36.94047083606137	39.0	35.0	41.0	31.0	41.0
46	36.901212673289876	39.0	35.0	41.0	31.0	41.0
47	36.84673916137664	39.0	35.0	41.0	31.0	41.0
48	36.784072988129466	39.0	35.0	41.0	31.0	41.0
49	36.79268410714794	39.0	35.0	40.0	31.0	41.0
50	36.7009564747075	39.0	35.0	40.0	31.0	41.0
51	35.73051325116001	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	4.0
12	5.0
13	4.0
14	6.0
15	7.0
16	6.0
17	18.0
18	36.0
19	62.0
20	114.0
21	220.0
22	352.0
23	529.0
24	752.0
25	1143.0
26	1687.0
27	2129.0
28	2588.0
29	3222.0
30	3996.0
31	5441.0
32	7397.0
33	10399.0
34	19463.0
35	30409.0
36	25214.0
37	40712.0
38	81282.0
39	114004.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.080617153918414	4.608443166614478	56.19630504711207	17.114634632355035
2	19.002248854223005	4.033989011927468	54.56745139343562	22.396310740413902
3	19.270403370434682	4.024879728998833	54.90278687124598	21.801930029320506
4	19.236243559452305	3.8557886646360555	53.01545731446953	23.89251046144211
5	18.05573742491958	5.468701073187395	51.8679723305531	24.60758917133992
6	20.34529875601355	5.578012468331008	53.975917333257414	20.10077144239802
7	82.10509835178912	1.3834723447863588	10.947650089669505	5.5637792137550175
8	82.86373082068947	2.0962737339520054	8.233653107119473	6.8063423382390615
9	75.86353155512539	6.257792706880355	11.467733211876228	6.410942526118022
10	29.21546300777136	40.549972956816305	17.00418457684534	13.230379458566995
11	27.21398274929545	25.25064761308321	29.713911583022572	17.821458054598764
12	25.81741581029918	21.94141592416522	31.59810982379231	20.64305844174329
13	21.544308121495064	26.72122747587463	34.0832360727604	17.65122832986991
14	17.91055822824447	29.321358421816733	31.74499701101654	21.02308633892226
15	15.143898203763273	26.08443166614478	38.98602294400638	19.78564718608557
16	16.010988072532665	26.322411682655357	33.50707392752427	24.15952631728771
17	16.192604400922313	24.78806683936349	30.683196219647584	28.33613254006661
18	18.7824874035697	23.35335477810356	34.99046371943408	22.873694098892653
19	20.73842124740243	24.53727689373452	31.85373907597711	22.870562782885933
20	23.019442625750806	26.18207179253608	31.79424407184947	19.004241509863647
21	21.859717042899028	27.674286202283017	31.992086310455747	18.473910444362208
22	20.158273790885023	24.454724017193772	30.103048763130175	25.28395342879103
23	18.68000797062256	26.288821201856017	29.19240513535825	25.838765692163168
24	20.13265393264824	24.594209912038487	30.62398588061146	24.649150274701814
25	17.86131116741154	26.932733638873863	28.40644481767201	26.799510376042583
26	16.7135415184036	28.77992541774602	28.09359788209172	26.412935181758662
27	20.80588687409263	27.165305018645565	28.927666600244812	23.101141507016994
28	18.531412792849213	24.754761023655668	32.90358393350223	23.810242249992882
29	18.74889692277036	22.41538330154573	33.49084801730764	25.344871758376268
30	21.351589854536137	24.485467847077913	32.46263770673802	21.700304591647924
31	24.18998548208033	25.587121751259644	28.876711548862765	21.346181217797263
32	24.158672322013153	24.894816248683423	28.653249452019697	22.293261977283727
33	23.046201144353667	23.177431751544308	31.149762304648583	22.626604799453442
34	20.548264966267187	21.732187081898147	32.54376725782117	25.175780694013493
35	20.19641891314868	21.471718523157506	31.16741154032281	27.164451023371004
36	25.32437587178684	22.03962538073956	31.0193856927325	21.616613054741094
37	23.511059238805547	23.160921176236158	33.54066440832361	19.787355176634687
38	21.054684164080957	26.015258048905466	31.58102991830112	21.349027868712458
39	19.494150132369267	23.50451194170059	36.55669105297618	20.44464687295397
40	22.64624669076831	21.6151897292835	33.526146488656096	22.212417091292096
41	20.308007629024452	21.754106293945174	30.182185658572692	27.755700418457685
42	24.14301574197956	19.682883088046914	29.482478863616958	26.69162230635657
43	24.22841526943551	19.987759401064647	30.85798058584076	24.925844743659084
44	21.49335307011301	21.25024908195508	31.163426229041534	26.092971618890378
45	20.684050214922145	21.573059295738563	30.518375131657606	27.22451535768169
46	23.605852714281646	21.628568988584927	32.66104927552734	22.10452902160608
47	19.848842836402973	21.49335307011301	35.233567707592016	23.424236385891998
48	20.470551396282275	20.0330211506163	34.667368840559085	24.829058612542344
49	21.618890375473256	17.342082040479376	38.00506703862905	23.033960545418317
50	21.79196675111731	19.409889265279396	33.59645876626149	25.201685217341797
51	20.36237866150474	18.607987702468044	30.391414500839765	30.638219135187448
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37.0
1	126.0
2	215.0
3	657.0
4	1099.0
5	721.5
6	344.0
7	407.5
8	471.0
9	541.0
10	611.0
11	674.0
12	737.0
13	793.0
14	849.0
15	850.5
16	852.0
17	895.0
18	938.0
19	841.5
20	745.0
21	956.0
22	1167.0
23	1224.5
24	1282.0
25	1455.5
26	2497.5
27	3366.0
28	4126.5
29	4887.0
30	5007.0
31	5127.0
32	6056.0
33	6985.0
34	7982.0
35	8979.0
36	9950.0
37	10921.0
38	11603.5
39	12286.0
40	14426.5
41	16567.0
42	20705.5
43	24844.0
44	27904.5
45	30965.0
46	36125.5
47	41286.0
48	46313.0
49	51340.0
50	47987.0
51	44634.0
52	35358.5
53	26083.0
54	21223.0
55	16363.0
56	13617.0
57	10871.0
58	9514.0
59	8157.0
60	7444.5
61	6732.0
62	5336.0
63	3940.0
64	3266.0
65	2592.0
66	2020.5
67	1449.0
68	1146.0
69	843.0
70	692.0
71	541.0
72	388.5
73	236.0
74	168.5
75	115.5
76	130.0
77	95.5
78	61.0
79	40.5
80	20.0
81	12.5
82	5.0
83	3.0
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	351290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.542731044111814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.31246851593976	18.999659601493175
2	14.957717771254622	8.837836662998138
3	6.404316981249599	5.676030422948849
4	3.302004304043493	3.9020090024342617
5	1.9114325275717936	2.823446853551017
6	1.2077772209935127	2.1408622560609687
7	0.8500204591251905	1.757834806414956
8	0.6387601371939793	1.5096575147857356
9	0.4434449387334209	1.1790517102086684
>10	5.577440987020963	38.9513923933731
>50	0.2969586231018942	5.387721503097811
>100	0.08769247930525735	4.217796075344644
>500	0.004982527233253259	0.958255477437483
>1k	0.004982527233253259	3.6584457198511835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	3413	0.9715619573571693	No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT	2889	0.82239744940078	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	2743	0.7808363460388853	No Hit
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	2312	0.6581456915938398	No Hit
TCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	1070	0.30459164792621485	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	748	0.21292948845683052	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	660	0.18787896040308577	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCT	633	0.18019300293205043	No Hit
TGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	624	0.177631017108372	No Hit
GCCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	590	0.1679524039966979	No Hit
TTCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTG	469	0.13350792792279884	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.846650915198269E-4	0.0	0.0	0.8574112556577187	0.0
2	2.846650915198269E-4	0.0	0.0	3.4464402630305444	0.0
3	2.846650915198269E-4	0.0	0.0	4.861510432975604	0.0
4	2.846650915198269E-4	0.0	0.0	6.2355888297418085	0.0
5	2.846650915198269E-4	0.0	0.0	8.414130775143045	0.0
6	2.846650915198269E-4	0.0	0.0	9.668649833470921	0.0
7	2.846650915198269E-4	0.0	0.0	10.799908907170714	0.0
8	2.846650915198269E-4	0.0	0.0	12.740755501152893	0.0
9	2.846650915198269E-4	0.0	0.0	13.660793076944975	0.0
10	2.846650915198269E-4	0.0	0.0	15.497167582339378	0.0
11	2.846650915198269E-4	0.0	0.0	18.295994762162316	0.0
12	2.846650915198269E-4	0.0	0.0	21.00059779669219	0.0
13	2.846650915198269E-4	0.0	0.0	21.85772438725839	0.0
14	2.846650915198269E-4	0.0	0.0	22.148652110791655	0.0
15	2.846650915198269E-4	0.0	0.0	22.58362037063395	0.0
16	2.846650915198269E-4	0.0	0.0	23.68612827009024	0.0
17	2.846650915198269E-4	0.0	0.0	25.382447550456888	0.0
18	2.846650915198269E-4	0.0	0.0	27.449970110165392	0.0
19	2.846650915198269E-4	0.0	0.0	28.533974778672892	0.0
20	5.693301830396538E-4	0.0	0.0	29.811267044322356	0.0
21	5.693301830396538E-4	0.0	0.0	31.31401406245552	0.0
22	5.693301830396538E-4	0.0	0.0	32.73677018987161	0.0
23	5.693301830396538E-4	0.0	0.0	34.119103874291895	0.0
24	5.693301830396538E-4	0.0	0.0	35.09493580802186	0.0
25	8.539952745594808E-4	0.0	0.0	35.90196134248057	0.0
26	8.539952745594808E-4	0.0	0.0	36.69816960346153	0.0
27	8.539952745594808E-4	0.0	0.0	37.43118221412508	0.0
28	8.539952745594808E-4	0.0	0.0	38.26553559736969	0.0
29	0.0011386603660793076	0.0	0.0	39.104158956987106	0.0
30	0.0011386603660793076	0.0	0.0	40.09393948020154	0.0
31	0.0011386603660793076	0.0	0.0	40.91292094850409	0.0
32	0.0011386603660793076	0.0	0.0	41.69461128981753	0.0
33	0.0011386603660793076	0.0	0.0	42.42193059865069	0.0
34	0.0011386603660793076	0.0	0.0	43.192234336303336	0.0
35	0.0011386603660793076	0.0	0.0	44.025449059181874	0.0
36	0.0011386603660793076	0.0	0.0	44.74508241054399	0.0
37	0.0011386603660793076	0.0	0.0	45.47809502120755	0.0
38	0.0011386603660793076	0.0	0.0	46.23274217882661	0.0
39	0.0011386603660793076	0.0	0.0	46.994221298642145	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	30	2.1611831E-6	45.000004	3
CGGAATA	20	7.0265896E-4	45.000004	6
CGTGGCA	25	3.8852555E-5	45.000004	15
CGTGGAG	20	7.0265896E-4	45.000004	12
TCGTTTG	50	2.1827873E-11	45.000004	1
TAATACA	20	7.0265896E-4	45.000004	18
CAAACCC	20	7.0265896E-4	45.000004	24
ACACGAC	25	3.8852555E-5	45.000004	26
CGACGGC	25	3.8852555E-5	45.000004	36
ACGTTCC	20	7.0265896E-4	45.000004	36
GCGCGAC	20	7.0265896E-4	45.000004	9
CGTGAGG	20	7.0265896E-4	45.000004	45
CGGTTGG	40	6.7921064E-9	45.000004	2
GCACGTG	25	3.8852555E-5	45.000004	12
CGACCAA	30	2.1611831E-6	45.000004	29
TTCGTAG	20	7.0265896E-4	45.000004	1
CGCGACC	20	7.0265896E-4	45.000004	10
TAGCCGC	20	7.0265896E-4	45.000004	14
GCCGCGG	20	7.0265896E-4	45.000004	1
ATCGTCA	30	2.1611831E-6	45.000004	40
>>END_MODULE