FastQCFastQC Report
Sat 14 Jan 2017
SRR2933937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179174
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC131601.1160354621116138No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC128681.0912723652319334No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG111880.9487997530474723No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC78470.6654658260782548TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT68840.5837984894510904TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC66170.5611555207289171TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC62360.5288447676085124TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31660.26849302986666934No Hit
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT30510.25874044034213783No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC28960.24559564576559523No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT27880.23643669212516558No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTAGCACT27760.23541903060956226No Hit
TCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG27430.23262046144165321No Hit
CGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG26380.2237159231801244No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA23210.19683269814293733No Hit
TTCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT17940.15214039658269263No Hit
GCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG14270.12101691523049185No Hit
TGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG12700.10770251040134873No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC12300.10431030534933776No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG207.033361E-445.012
ACACGCG453.8562575E-1045.036
TTCACGA207.033361E-445.024
CTAAGCG207.033361E-445.034
ATCGTGA207.033361E-445.030
TTAACGC253.8908674E-545.043
TTACGCG551.8189894E-1245.01
TGTCGAA207.033361E-445.041
AGTCGAA207.033361E-445.023
CGATGAA4700.042.60638419
TCGACGT501.0822987E-940.526
TCGTTCA451.9283107E-840.016
ATTCGCG451.9283107E-840.01
CGTTTTT22950.039.9019621
TGTTCCG1600.039.3751
TCGTGCG1200.039.3749961
ACGGCTG16850.039.258168
TACGGCT16800.039.241077
CGTGCGG1550.039.1935462
CCGATGA5100.038.8235318
Produced by FastQC (version 0.11.5)