##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933937.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1179174
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2453547992069	33.0	31.0	34.0	30.0	34.0
2	32.44221633109278	34.0	31.0	34.0	30.0	34.0
3	32.51816186584847	34.0	31.0	34.0	30.0	34.0
4	35.9728258933796	37.0	35.0	37.0	35.0	37.0
5	36.00605423796657	37.0	35.0	37.0	35.0	37.0
6	35.53324700171476	37.0	35.0	37.0	33.0	37.0
7	35.950947866896655	37.0	35.0	37.0	35.0	37.0
8	35.90597740452215	37.0	35.0	37.0	35.0	37.0
9	37.82687372686304	39.0	38.0	39.0	35.0	39.0
10	37.400986622839376	39.0	37.0	39.0	35.0	39.0
11	37.34435206339353	39.0	37.0	39.0	34.0	39.0
12	37.31956776523227	39.0	37.0	39.0	34.0	39.0
13	37.27328027924632	39.0	37.0	39.0	34.0	39.0
14	38.51208133829274	40.0	38.0	41.0	34.0	41.0
15	38.495385753078004	40.0	38.0	41.0	34.0	41.0
16	38.60120898188054	40.0	38.0	41.0	34.0	41.0
17	38.51252062884697	40.0	38.0	41.0	34.0	41.0
18	38.483738617031925	40.0	38.0	41.0	34.0	41.0
19	38.47837214863964	40.0	38.0	41.0	34.0	41.0
20	38.36455434058078	40.0	38.0	41.0	34.0	41.0
21	38.34745253881107	40.0	38.0	41.0	34.0	41.0
22	38.37710041096564	40.0	38.0	41.0	34.0	41.0
23	38.27539277494246	40.0	37.0	41.0	34.0	41.0
24	38.2381412751638	40.0	37.0	41.0	34.0	41.0
25	38.165320809312284	40.0	37.0	41.0	34.0	41.0
26	38.09939160802392	40.0	37.0	41.0	34.0	41.0
27	38.10351652936717	40.0	37.0	41.0	34.0	41.0
28	38.040211198686535	40.0	37.0	41.0	34.0	41.0
29	38.00351178028009	40.0	37.0	41.0	34.0	41.0
30	37.959659049470226	40.0	37.0	41.0	33.0	41.0
31	37.89189466524873	40.0	37.0	41.0	33.0	41.0
32	37.71671356390151	40.0	37.0	41.0	33.0	41.0
33	37.68531870614515	40.0	37.0	41.0	33.0	41.0
34	37.60760583255737	40.0	37.0	41.0	33.0	41.0
35	37.55766578978166	40.0	37.0	41.0	33.0	41.0
36	37.50329976746434	40.0	37.0	41.0	33.0	41.0
37	37.4479440693231	40.0	37.0	41.0	33.0	41.0
38	37.36880986181853	40.0	36.0	41.0	32.0	41.0
39	37.339640290576284	40.0	36.0	41.0	33.0	41.0
40	37.21396163755307	40.0	36.0	41.0	32.0	41.0
41	37.14270073797421	39.0	36.0	41.0	32.0	41.0
42	37.13009191179589	39.0	36.0	41.0	32.0	41.0
43	37.089140364356744	39.0	36.0	41.0	32.0	41.0
44	37.03032800926751	39.0	36.0	41.0	31.0	41.0
45	37.0460067810179	39.0	36.0	41.0	32.0	41.0
46	36.952264890508104	39.0	35.0	41.0	31.0	41.0
47	36.796333704779784	39.0	35.0	41.0	31.0	41.0
48	36.794586719178	39.0	35.0	41.0	31.0	41.0
49	36.842105575597834	39.0	35.0	41.0	31.0	41.0
50	36.7593281398674	39.0	35.0	41.0	31.0	41.0
51	35.833784496605254	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	15.0
11	9.0
12	11.0
13	12.0
14	10.0
15	25.0
16	28.0
17	66.0
18	126.0
19	290.0
20	654.0
21	1137.0
22	1935.0
23	2786.0
24	3899.0
25	5523.0
26	7358.0
27	8567.0
28	9700.0
29	11283.0
30	14248.0
31	18650.0
32	24915.0
33	34648.0
34	56996.0
35	76955.0
36	87643.0
37	139510.0
38	272455.0
39	399522.0
40	186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.065640863858935	4.1719881883420085	55.402425765832696	13.35994518196636
2	17.871493096014667	9.617918983966742	51.62232206612425	20.888265853894335
3	17.856058563028018	9.50614582750298	51.244176007951324	21.39361960151767
4	17.01988001770731	3.971932895399661	55.877673693619435	23.130513393273596
5	21.017169645870755	4.936336791686384	50.620179888633906	23.426313673808956
6	19.146877390444498	9.277087181365939	53.540868438415366	18.035166989774197
7	78.5061407391954	1.3431435903437492	14.929688069784442	5.221027600676406
8	79.27506881936	5.393351617318563	9.32093143166318	6.010648131658263
9	74.56058223807513	3.89747399450802	11.320297089318455	10.221646678098399
10	35.65572171706635	26.34751105434821	21.17312627313696	16.823640955448475
11	25.099688425965972	24.547352638372285	28.853756951900227	21.499201983761516
12	21.55067869542578	21.045833778560247	33.70096355584503	23.702523970168947
13	21.74768100382132	23.20522670954414	36.49190026238706	18.555192024247482
14	20.309131646389762	26.433333842164092	31.301148091799853	21.956386419646297
15	16.24789895299591	26.049845061034247	36.58145447576015	21.120801510209688
16	17.82256053813941	25.47647760211809	31.44183979633201	25.259122063410487
17	17.988100144677546	26.546972711406458	30.800713041501933	24.66421410241406
18	19.157647641484633	25.08908778517844	32.03250750101342	23.720757072323508
19	20.279110631679465	27.773678863339928	29.402191703684107	22.5450188012965
20	22.04789115092429	26.63101459157003	31.923617718843865	19.39747653866181
21	22.009389623583967	29.177882144619876	29.625059575601227	19.18766865619493
22	20.021218242600327	24.707719132206105	29.406516765125417	25.86454586006815
23	19.38272044668556	27.6709798553903	28.92253390932975	24.02376578859439
24	21.199500667416345	24.748849618461737	28.73341847768014	25.31823123644178
25	18.5602803318255	29.975304747221358	26.329108341941055	25.13530657901209
26	17.478590945865495	28.570507830057313	29.198913815942344	24.751987408134845
27	19.738308341262613	27.679375562894027	28.11332339417253	24.46899270167083
28	16.34703614564093	26.628385632654723	32.75733691550187	24.26724130620248
29	18.129300679967503	26.65484483206041	29.699433671366567	25.516420816605525
30	20.11170531236272	27.111520437187387	28.929827150191574	23.846947100258316
31	18.86032086867587	28.394028362226443	27.465581839491033	25.280068929606657
32	19.11337936555589	32.994282438384836	24.7368920956534	23.15544610040588
33	19.954391803075712	26.58691592589389	24.8604531646729	28.598239106357504
34	18.074262152998624	27.93353652641595	26.87720387321973	27.114997447365695
35	16.746722705894125	28.786930512375612	25.05075586809071	29.41559091363955
36	18.333935449730067	28.49053659595615	25.990481472624055	27.185046481689724
37	15.999759153441307	28.52708760539157	29.63116554469484	25.841987696472273
38	18.38473372038393	28.78582804573371	28.081860692315132	24.747577541567235
39	20.395208849584538	26.96777574810842	27.373653082581534	25.263362319725502
40	21.36716040211199	26.06884140932551	27.630103784513565	24.933894404048935
41	16.94567553219457	27.800392478124518	25.33917810263795	29.914753887042966
42	17.61529680946154	27.871543979090447	27.424875378866904	27.088283832581112
43	19.198184491856164	25.16346188094378	27.416394866236875	28.221958760963183
44	19.023061906046095	24.799732694241904	27.990695181542335	28.18651021816967
45	19.501108403000742	23.686071775666694	27.026969726266014	29.785850095066547
46	21.675342231087185	26.01948482581875	26.92028487738027	25.384888065713795
47	16.251969599058324	25.75904828295061	32.40836382077624	25.58061829721483
48	16.94898293212028	25.902114531019173	28.89666834580817	28.252234191052384
49	18.746766804559805	23.434030940302282	32.034966849676124	25.784235405461793
50	19.791141934947685	22.413146829899574	28.493589580502963	29.30212165464978
51	18.389567612583047	22.441556547210165	25.702228848329423	33.46664699187736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	131.0
1	448.0
2	765.0
3	3214.0
4	5663.0
5	3996.5
6	2330.0
7	2247.5
8	2165.0
9	2258.5
10	2352.0
11	2490.5
12	2629.0
13	2618.0
14	2607.0
15	2792.0
16	2977.0
17	3077.0
18	3177.0
19	3236.5
20	3296.0
21	3481.0
22	3666.0
23	4448.5
24	5231.0
25	5930.5
26	8071.5
27	9513.0
28	11471.0
29	13429.0
30	15329.5
31	17230.0
32	20013.0
33	22796.0
34	26013.5
35	29231.0
36	32595.5
37	35960.0
38	41162.0
39	46364.0
40	52189.5
41	58015.0
42	65043.5
43	72072.0
44	81871.0
45	91670.0
46	132533.0
47	173396.0
48	168899.5
49	164403.0
50	150886.5
51	137370.0
52	110007.0
53	82644.0
54	67676.0
55	52708.0
56	44546.0
57	36384.0
58	32154.5
59	27925.0
60	24994.0
61	22063.0
62	18529.5
63	14996.0
64	12254.0
65	9512.0
66	8065.5
67	6619.0
68	5468.5
69	4318.0
70	3435.0
71	2552.0
72	2390.5
73	2229.0
74	1754.0
75	817.0
76	355.0
77	300.5
78	246.0
79	184.0
80	122.0
81	101.5
82	81.0
83	65.5
84	50.0
85	34.0
86	18.0
87	10.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1179174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.18940396081522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60670012900482	25.1979709714051
2	12.8627730923998	9.052666368095219
3	4.875261914572352	5.146726827799918
4	2.4266405146082333	3.415681333449186
5	1.471270555687658	2.588656695987304
6	0.9276000061005433	1.9585014797236011
7	0.6785432333584431	1.6714272360469578
8	0.49193898349367376	1.3848831691385355
9	0.4084245061090344	1.293499343967053
>10	4.006754645600801	30.946645640590603
>50	0.17137741210260982	3.893532349770428
>100	0.06277728939043527	4.119323204211389
>500	0.004847667108133609	1.2148318746601774
>1k	0.0033933669756935265	2.5886503823587996
>5k	9.695334216267219E-4	2.3527292305431673
>10k+	7.271500662200413E-4	3.174273892252556
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	13160	1.1160354621116138	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	12868	1.0912723652319334	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG	11188	0.9487997530474723	No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	7847	0.6654658260782548	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	6884	0.5837984894510904	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	6617	0.5611555207289171	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	6236	0.5288447676085124	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3166	0.26849302986666934	No Hit
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	3051	0.25874044034213783	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC	2896	0.24559564576559523	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	2788	0.23643669212516558	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTAGCACT	2776	0.23541903060956226	No Hit
TCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG	2743	0.23262046144165321	No Hit
CGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG	2638	0.2237159231801244	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA	2321	0.19683269814293733	No Hit
TTCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	1794	0.15214039658269263	No Hit
GCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG	1427	0.12101691523049185	No Hit
TGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG	1270	0.10770251040134873	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC	1230	0.10431030534933776	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6043213300157568	0.0
2	0.0	0.0	0.0	2.4769033238521203	0.0
3	0.0	0.0	0.0	3.564274653274241	0.0
4	0.0	0.0	0.0	4.9119129153119045	0.0
5	0.0	0.0	0.0	7.684616519699383	0.0
6	0.0	0.0	0.0	9.002997013163451	0.0
7	0.0	0.0	0.0	10.216727980772982	0.0
8	0.0	0.0	0.0	12.000519007372958	0.0
9	0.0	0.0	0.0	12.69914363783462	0.0
10	0.0	0.0	0.0	15.134238034420704	0.0
11	0.0	0.0	0.0	17.746320729595464	0.0
12	0.0	0.0	0.0	21.15184018643559	0.0
13	0.0	0.0	0.0	22.051452966228904	0.0
14	0.0	0.0	0.0	22.36828491808673	0.0
15	0.0	0.0	0.0	22.948097566601707	0.0
16	0.0	0.0	0.0	23.936586118757706	0.0
17	0.0	0.0	0.0	25.345453681984168	0.0
18	0.0	0.0	0.0	26.880087247513938	0.0
19	0.0	0.0	0.0	28.21237578169125	0.0
20	0.0	0.0	0.0	29.275153624486293	0.0
21	0.0	0.0	0.0	30.87356064499387	0.0
22	0.0	0.0	0.0	32.58967718080623	0.0
23	8.48051263002746E-5	0.0	0.0	34.36329159225017	0.0
24	8.48051263002746E-5	0.0	0.0	35.55030894507511	0.0
25	8.48051263002746E-5	0.0	0.0	36.60553913162943	0.0
26	8.48051263002746E-5	0.0	0.0	37.50074204485513	0.0
27	8.48051263002746E-5	0.0	0.0	38.4193511729397	0.0
28	8.48051263002746E-5	0.0	0.0	39.38333104359492	0.0
29	8.48051263002746E-5	0.0	0.0	40.37767114946564	0.0
30	8.48051263002746E-5	0.0	0.0	41.379558911577085	0.0
31	8.48051263002746E-5	0.0	0.0	42.37415343282671	0.0
32	8.48051263002746E-5	0.0	0.0	43.29581554545809	0.0
33	8.48051263002746E-5	0.0	0.0	44.206622601923044	0.0
34	8.48051263002746E-5	0.0	0.0	45.06697060823932	0.0
35	8.48051263002746E-5	0.0	0.0	45.944618860320865	0.0
36	8.48051263002746E-5	0.0	0.0	46.82591373283332	0.0
37	8.48051263002746E-5	0.0	0.0	47.67710278550918	0.0
38	8.48051263002746E-5	0.0	0.0	48.4852956391508	0.0
39	8.48051263002746E-5	0.0	0.0	49.276357857279756	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	20	7.033361E-4	45.0	12
ACACGCG	45	3.8562575E-10	45.0	36
TTCACGA	20	7.033361E-4	45.0	24
CTAAGCG	20	7.033361E-4	45.0	34
ATCGTGA	20	7.033361E-4	45.0	30
TTAACGC	25	3.8908674E-5	45.0	43
TTACGCG	55	1.8189894E-12	45.0	1
TGTCGAA	20	7.033361E-4	45.0	41
AGTCGAA	20	7.033361E-4	45.0	23
CGATGAA	470	0.0	42.606384	19
TCGACGT	50	1.0822987E-9	40.5	26
TCGTTCA	45	1.9283107E-8	40.0	16
ATTCGCG	45	1.9283107E-8	40.0	1
CGTTTTT	2295	0.0	39.901962	1
TGTTCCG	160	0.0	39.375	1
TCGTGCG	120	0.0	39.374996	1
ACGGCTG	1685	0.0	39.25816	8
TACGGCT	1680	0.0	39.24107	7
CGTGCGG	155	0.0	39.193546	2
CCGATGA	510	0.0	38.82353	18
>>END_MODULE