##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933935.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1466949
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47021811937566	34.0	31.0	34.0	31.0	34.0
2	32.70171355650401	34.0	31.0	34.0	31.0	34.0
3	32.75337452085928	34.0	31.0	34.0	31.0	34.0
4	36.10616660838243	37.0	35.0	37.0	35.0	37.0
5	36.147688842625065	37.0	35.0	37.0	35.0	37.0
6	35.79543801454584	37.0	35.0	37.0	35.0	37.0
7	36.107183003635434	37.0	35.0	37.0	35.0	37.0
8	36.11818270437486	37.0	37.0	37.0	35.0	37.0
9	37.95931078721892	39.0	39.0	39.0	35.0	39.0
10	37.62051509629851	39.0	37.0	39.0	35.0	39.0
11	37.56075569089314	39.0	37.0	39.0	35.0	39.0
12	37.4558883778509	39.0	37.0	39.0	35.0	39.0
13	37.44053474251661	39.0	37.0	39.0	35.0	39.0
14	38.71226402553872	40.0	38.0	41.0	35.0	41.0
15	38.75794659528041	40.0	38.0	41.0	35.0	41.0
16	38.80396455500498	40.0	38.0	41.0	35.0	41.0
17	38.76813304348004	40.0	38.0	41.0	35.0	41.0
18	38.707053892125764	40.0	38.0	41.0	35.0	41.0
19	38.73895752340402	40.0	38.0	41.0	35.0	41.0
20	38.699682129371915	40.0	38.0	41.0	35.0	41.0
21	38.618906315079805	40.0	38.0	41.0	35.0	41.0
22	38.607881391922966	40.0	38.0	41.0	35.0	41.0
23	38.528417825023226	40.0	38.0	41.0	34.0	41.0
24	38.4952162617787	40.0	38.0	41.0	34.0	41.0
25	38.36621723045587	40.0	38.0	41.0	34.0	41.0
26	38.309601083609586	40.0	37.0	41.0	34.0	41.0
27	38.307939812495185	40.0	38.0	41.0	34.0	41.0
28	38.23230732629423	40.0	37.0	41.0	34.0	41.0
29	38.235423317375044	40.0	37.0	41.0	34.0	41.0
30	38.1908675761734	40.0	37.0	41.0	34.0	41.0
31	38.122765004100344	40.0	37.0	41.0	34.0	41.0
32	38.048941033396524	40.0	37.0	41.0	34.0	41.0
33	37.95735161890427	40.0	37.0	41.0	34.0	41.0
34	37.91635155687076	40.0	37.0	41.0	34.0	41.0
35	37.87101051229457	40.0	37.0	41.0	33.0	41.0
36	37.82713577636305	40.0	37.0	41.0	33.0	41.0
37	37.809636872174835	40.0	37.0	41.0	33.0	41.0
38	37.77767461581827	40.0	37.0	41.0	33.0	41.0
39	37.733982571991255	40.0	37.0	41.0	33.0	41.0
40	37.65719667145893	40.0	36.0	41.0	33.0	41.0
41	37.53169128579112	40.0	36.0	41.0	33.0	41.0
42	37.49111591473187	40.0	36.0	41.0	33.0	41.0
43	37.35947398307644	40.0	36.0	41.0	33.0	41.0
44	37.283485656283894	39.0	36.0	41.0	32.0	41.0
45	37.31554948399706	39.0	36.0	41.0	33.0	41.0
46	37.242516951850405	39.0	35.0	41.0	32.0	41.0
47	37.18621915281308	39.0	35.0	41.0	32.0	41.0
48	37.14423541650051	39.0	35.0	41.0	32.0	41.0
49	37.13597746070245	39.0	35.0	41.0	32.0	41.0
50	37.088494555707115	39.0	35.0	41.0	32.0	41.0
51	36.15384243078662	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	17.0
10	21.0
11	23.0
12	21.0
13	22.0
14	21.0
15	22.0
16	52.0
17	84.0
18	181.0
19	329.0
20	544.0
21	928.0
22	1453.0
23	2221.0
24	3322.0
25	4858.0
26	6935.0
27	8556.0
28	10114.0
29	12428.0
30	15495.0
31	19988.0
32	26529.0
33	38228.0
34	70439.0
35	96466.0
36	97620.0
37	157954.0
38	324221.0
39	567442.0
40	406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.90825993269023	4.448143732331526	56.39575745305392	17.24783888192432
2	19.477977762008088	4.852043254400801	54.175162190369264	21.49481679322185
3	20.133351602543783	4.778352894340567	52.206586595716686	22.881708907398963
4	19.312600506220733	4.64290169596898	51.90057732068395	24.143920477126336
5	18.883614904130955	5.487375498398377	50.08892606355096	25.54008353391972
6	21.692437842079034	6.265043979034036	53.424352175842515	18.618166003044415
7	83.90891571554295	2.1754675861260346	8.987906191694464	4.927710506636564
8	85.67325789785465	2.8552458197251576	6.748019188124468	4.723477094295712
9	79.70767899906541	5.659842298539349	8.974545127335714	5.657933575059528
10	35.60157851431781	36.84592988576972	14.851027540834751	12.701464059077718
11	25.932394377718655	24.767664042853568	29.97132142971569	19.32862014971209
12	25.20728396147378	22.291777014742845	31.979639373965966	20.521299649817408
13	23.689985132407465	22.62859854023555	32.83658804770991	20.844828279647075
14	19.444711438502633	26.088705196976854	31.623185264109388	22.843398100411125
15	17.93620637118264	26.366628969377942	34.794392988440634	20.90277167099879
16	20.77993168133316	25.813917184578333	32.08741408188015	21.31873705220836
17	21.148587987721452	25.597277069618645	29.808261909582406	23.445873033077497
18	20.420273642778312	25.443829335580176	31.308859408200284	22.82703761344123
19	22.789544830801887	25.700143631441858	29.82591760177075	21.684393935985504
20	24.666569867118763	26.260353972769334	29.563468123295355	19.50960803681655
21	24.61748840620908	26.27657812234781	29.40115845881486	19.70477501262825
22	23.16010986067	24.086317929253166	29.519499314563763	23.23407289551307
23	21.7292489377613	25.690122833172797	29.347100683118498	23.233527545947403
24	22.474332781848585	23.719774852431815	30.04664783847291	23.759244527246686
25	20.5782205107335	26.382375938086465	28.177803045640985	24.861600505539048
26	20.160755418218358	26.935360397668905	28.549595112031845	24.3542890720809
27	19.940843205864688	26.357494364153084	30.218841963831057	23.482820466151175
28	18.100424759142957	26.130356270054378	31.42229211785822	24.346926852944446
29	19.56400665599145	23.531765589669444	31.4497641022285	25.454463652110604
30	20.499826510669422	23.778331762044896	31.11791889152247	24.603922835763207
31	20.172753108662945	26.27173814495255	28.731332854789088	24.824175891595413
32	20.156665296475886	26.41073411550095	28.504331098081803	24.928269489941368
33	20.507393235892998	25.677920636641083	28.86739757142205	24.94728855604387
34	19.525900355090737	24.64489222188365	30.24092862124041	25.5882788017852
35	18.601873684770226	23.813711315117295	29.856729852230718	27.727685147881758
36	18.956759914625525	26.067368395220285	29.241507373466973	25.73436431668722
37	18.904065512843324	25.89081147333684	31.363189858679476	23.841933155140364
38	18.848235351058555	25.4034734677211	29.72455075125311	26.02374042996723
39	19.63422041257058	24.58626714357486	30.529895722346172	25.24961672150838
40	20.612441195978864	23.12070835455084	30.991125117505792	25.275725331964505
41	18.12101170524674	24.77332204459732	30.189393087285243	26.91627316287069
42	19.780033252689766	25.183288580584602	28.469565063270775	26.567113103454858
43	19.819298421417514	25.063107170051584	29.316152095267118	25.801442313263788
44	19.78221465095242	23.60238835842282	29.777858671296688	26.837538319328075
45	20.057275338133774	21.810710529132233	29.162295349054396	28.9697187836796
46	20.12183109296915	23.33121328689682	30.32252655000276	26.224429070131272
47	17.55896080913515	23.827617729041705	32.33752502643241	26.275896435390734
48	17.699729165771952	22.824924383874286	32.289261589871224	27.18608486048254
49	18.846599302361568	22.248489892968333	32.812388160733605	26.092522643936494
50	19.21464209048849	21.946570739678066	30.73153872425013	28.107248445583316
51	17.697070586639345	21.968930071870254	29.60675524506987	30.72724409642053
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	778.0
2	1348.0
3	2750.5
4	4153.0
5	2958.0
6	1763.0
7	1819.5
8	1876.0
9	1877.5
10	1879.0
11	1893.0
12	1907.0
13	1990.0
14	2073.0
15	2149.5
16	2226.0
17	2454.5
18	2683.0
19	3116.5
20	3550.0
21	4157.0
22	4764.0
23	5710.0
24	6656.0
25	9392.5
26	14149.5
27	16170.0
28	18882.0
29	21594.0
30	24535.5
31	27477.0
32	31081.0
33	34685.0
34	40395.0
35	46105.0
36	50433.0
37	54761.0
38	59256.5
39	63752.0
40	70184.0
41	76616.0
42	81657.0
43	86698.0
44	96232.0
45	105766.0
46	120092.0
47	134418.0
48	149756.5
49	165095.0
50	157720.0
51	150345.0
52	127965.0
53	105585.0
54	91839.0
55	78093.0
56	70661.0
57	63229.0
58	58619.5
59	54010.0
60	49012.5
61	44015.0
62	38649.5
63	33284.0
64	27265.0
65	21246.0
66	17095.0
67	12944.0
68	11073.0
69	9202.0
70	7418.5
71	5635.0
72	4864.0
73	4093.0
74	3178.5
75	1781.5
76	1299.0
77	1016.0
78	733.0
79	522.5
80	312.0
81	235.0
82	158.0
83	126.0
84	94.0
85	55.5
86	17.0
87	18.5
88	20.0
89	17.5
90	15.0
91	7.5
92	0.0
93	2.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1466949.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.468332086396895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.29389494126933	18.75919385720287
2	12.869189614628528	7.06990348034853
3	5.233454683772666	4.312628136389305
4	2.5715007060054313	2.8253934141184502
5	1.5384429272459441	2.1129230610780065
6	0.971430680395185	1.6010148316805657
7	0.6759113640960611	1.299631046697209
8	0.5142663493458589	1.1300831091752843
9	0.42189580446518743	1.0429896656616604
>10	6.283209599560424	44.58154130873033
>50	0.5528539301137286	9.100251465156708
>100	0.06793317694919966	3.2860385634000795
>500	0.0030081110762950987	0.5840407905648548
>1k	0.002256083307221324	1.1389552837459638
>5k	7.520277690737747E-4	1.1554119860502552
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	6560	0.4471866438437873	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	5203	0.3546817237681746	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	5017	0.3420023463665063	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	4462	0.30416872024862485	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	2099	0.14308609229087035	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1983	0.13517852358875462	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	1676	0.11425073400643103	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	1485	0.1012305131262232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.090121742473665E-4	0.0	0.0	0.37029235508528247	0.0
2	4.090121742473665E-4	0.0	0.0	1.5420440656082794	0.0
3	4.090121742473665E-4	0.0	0.0	2.2798338592548206	0.0
4	4.090121742473665E-4	0.0	0.0	3.0286669816060408	0.0
5	4.090121742473665E-4	0.0	0.0	4.258975601742119	0.0
6	4.090121742473665E-4	0.0	0.0	5.022669499757661	0.0
7	4.090121742473665E-4	0.0	0.0	5.713082049887215	0.0
8	4.090121742473665E-4	0.0	0.0	6.792874189900262	0.0
9	4.090121742473665E-4	0.0	0.0	7.280621207690247	0.0
10	4.090121742473665E-4	0.0	0.0	8.26504534240795	0.0
11	4.090121742473665E-4	0.0	0.0	10.113712201310339	0.0
12	4.090121742473665E-4	0.0	0.0	12.026934815048103	0.0
13	4.090121742473665E-4	0.0	0.0	12.579714768543418	0.0
14	4.090121742473665E-4	0.0	0.0	12.785856904364092	0.0
15	4.090121742473665E-4	0.0	0.0	13.106317942886903	0.0
16	4.090121742473665E-4	0.0	0.0	13.809137195635294	0.0
17	4.090121742473665E-4	0.0	0.0	14.78381320686677	0.0
18	4.090121742473665E-4	0.0	0.0	15.831293385114275	0.0
19	4.090121742473665E-4	0.0	0.0	16.667791450145845	0.0
20	4.090121742473665E-4	0.0	0.0	17.51083370996538	0.0
21	4.090121742473665E-4	0.0	0.0	18.64066167262802	0.0
22	4.090121742473665E-4	0.0	0.0	19.862926386670566	0.0
23	7.498556527868385E-4	0.0	0.0	20.959147182349216	0.0
24	7.498556527868385E-4	0.0	0.0	21.78432924389328	0.0
25	7.498556527868385E-4	0.0	0.0	22.517347228840265	0.0
26	7.498556527868385E-4	0.0	0.0	23.197261799830805	0.0
27	7.498556527868385E-4	0.0	0.0	23.837025009049395	0.0
28	7.498556527868385E-4	0.0	0.0	24.520757026999576	0.0
29	7.498556527868385E-4	0.0	0.0	25.22221290583381	0.0
30	7.498556527868385E-4	0.0	0.0	26.041805134329824	0.0
31	7.498556527868385E-4	0.0	0.0	26.81463363757022	0.0
32	7.498556527868385E-4	0.0	0.0	27.53217732859152	0.0
33	7.498556527868385E-4	0.0	0.0	28.225725638723638	0.0
34	7.498556527868385E-4	0.0	0.0	28.913820453199122	0.0
35	7.498556527868385E-4	0.0	0.0	29.623388406822595	0.0
36	8.18024348494733E-4	0.0	0.0	30.339909567408274	0.0
37	8.18024348494733E-4	0.0	0.0	31.03618462536871	0.0
38	8.18024348494733E-4	0.0	0.0	31.739412890291348	0.0
39	8.18024348494733E-4	0.0	0.0	32.44686761434787	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAA	30	2.1659125E-6	45.000004	20
TTATCCG	20	7.0339255E-4	45.0	1
TCGTTTG	440	0.0	39.375004	1
CTACGGG	190	0.0	39.078945	3
TACGGGA	415	0.0	39.036144	4
TGGGCGA	1630	0.0	38.2362	6
TTTGGGA	11065	0.0	38.228645	4
TTGGGAC	4055	0.0	37.786682	5
TGTCACG	60	1.5643309E-10	37.500004	1
AACCGGT	30	1.1400755E-4	37.500004	38
CGTTATA	30	1.1400755E-4	37.500004	26
ATCGTCG	30	1.1400755E-4	37.500004	1
CGTTTTT	1445	0.0	37.370243	1
CTTTTCG	410	0.0	37.317074	1
TTGGGAT	7160	0.0	37.23813	5
GGGCGAT	2605	0.0	37.226486	7
GGCCGAT	425	0.0	37.058823	8
TCTGGGA	5000	0.0	36.81	4
GGCGATT	1375	0.0	36.654545	8
CTTGGGA	4745	0.0	36.51212	4
>>END_MODULE