##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933934.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	840192
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.437360746115175	33.0	31.0	34.0	31.0	34.0
2	32.669451744363194	34.0	31.0	34.0	31.0	34.0
3	32.71598396556978	34.0	31.0	34.0	31.0	34.0
4	36.088580943403414	37.0	35.0	37.0	35.0	37.0
5	36.13544642177026	37.0	35.0	37.0	35.0	37.0
6	35.789122010207194	37.0	35.0	37.0	35.0	37.0
7	36.06110627094759	37.0	35.0	37.0	35.0	37.0
8	36.05914600472273	37.0	36.0	37.0	35.0	37.0
9	37.91141310557587	39.0	38.0	39.0	35.0	39.0
10	37.67016586684948	39.0	37.0	39.0	35.0	39.0
11	37.63417290333638	39.0	37.0	39.0	35.0	39.0
12	37.43557782030774	39.0	37.0	39.0	35.0	39.0
13	37.33918199649604	39.0	37.0	39.0	35.0	39.0
14	38.404669408897014	40.0	38.0	41.0	34.0	41.0
15	38.52291261997258	40.0	38.0	41.0	34.0	41.0
16	38.636752075716025	40.0	38.0	41.0	35.0	41.0
17	38.6252880294028	40.0	38.0	41.0	35.0	41.0
18	38.60351324459171	40.0	38.0	41.0	35.0	41.0
19	38.6186978690585	40.0	38.0	41.0	35.0	41.0
20	38.56927821259902	40.0	38.0	41.0	34.0	41.0
21	38.48369658363803	40.0	38.0	41.0	34.0	41.0
22	38.48579372333943	40.0	38.0	41.0	34.0	41.0
23	38.44942465531688	40.0	38.0	41.0	34.0	41.0
24	38.41916609536868	40.0	37.0	41.0	34.0	41.0
25	38.311438337903716	40.0	37.0	41.0	34.0	41.0
26	38.23585918456734	40.0	37.0	41.0	34.0	41.0
27	38.197884531154784	40.0	37.0	41.0	34.0	41.0
28	38.140356013863496	40.0	37.0	41.0	34.0	41.0
29	38.099452268053014	40.0	37.0	41.0	34.0	41.0
30	38.00078434453077	40.0	37.0	41.0	34.0	41.0
31	37.93568374847654	40.0	36.0	41.0	34.0	41.0
32	37.84693617649299	40.0	36.0	41.0	34.0	41.0
33	37.75481437576173	40.0	36.0	41.0	33.0	41.0
34	37.683696107556365	40.0	36.0	41.0	33.0	41.0
35	37.62266958028641	40.0	36.0	41.0	33.0	41.0
36	37.49698997372029	40.0	36.0	41.0	33.0	41.0
37	37.39639391948507	40.0	35.0	41.0	33.0	41.0
38	37.34587451439671	40.0	35.0	41.0	33.0	41.0
39	37.28898870734309	39.0	35.0	41.0	33.0	41.0
40	37.16934462599025	39.0	35.0	41.0	32.0	41.0
41	37.05708814175807	39.0	35.0	41.0	32.0	41.0
42	37.048651974786715	39.0	35.0	41.0	32.0	41.0
43	36.90753542047532	39.0	35.0	41.0	32.0	41.0
44	36.760596387492384	39.0	35.0	41.0	31.0	41.0
45	36.77045603861974	39.0	35.0	41.0	31.0	41.0
46	36.70219187995125	39.0	35.0	41.0	31.0	41.0
47	36.60481651812919	39.0	35.0	41.0	31.0	41.0
48	36.53842931139549	39.0	35.0	40.0	31.0	41.0
49	36.50669489830896	39.0	35.0	40.0	31.0	41.0
50	36.40919694546009	38.0	35.0	40.0	31.0	41.0
51	35.41309724443936	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	16.0
10	28.0
11	27.0
12	26.0
13	21.0
14	16.0
15	34.0
16	52.0
17	99.0
18	177.0
19	274.0
20	472.0
21	793.0
22	1108.0
23	1615.0
24	2163.0
25	3069.0
26	4164.0
27	5294.0
28	6174.0
29	7540.0
30	9572.0
31	12587.0
32	16984.0
33	25645.0
34	50395.0
35	57321.0
36	62431.0
37	98903.0
38	186317.0
39	286690.0
40	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.97428206886045	4.664410039609994	54.49492496953078	16.86638292199878
2	20.729428511578305	4.46957362126752	52.72449630560634	22.076501561547836
3	20.745496267519805	4.38245067794028	51.97193022547227	22.900122829067644
4	19.686452620353442	4.7209447364411945	50.543447212065814	25.049155431139546
5	18.845811433577087	5.459466407678245	49.61496895947593	26.07975319926874
6	21.35440470749543	6.059686357404022	52.416947554844604	20.168961380255944
7	79.73796465569775	2.4624133531383303	11.582233584704449	6.2173884064594755
8	80.06824630560634	3.3339998476538693	9.68564328153565	6.912110565204143
9	73.7940851614869	5.96589827087142	13.011906802254721	7.228109765386959
10	36.35657087903717	30.397813833028643	18.723220406764167	14.522394881170017
11	27.8262587599025	24.61830153107861	26.739721397014016	20.815718312004876
12	25.453348758379036	21.31322364411944	32.14146290371724	21.09196469378428
13	24.809805377818403	23.22362031535649	31.197988078915294	20.76858622790981
14	19.19954010511883	25.818622410115783	32.11623057586837	22.865606908897014
15	16.576211151736747	24.955367344606945	34.212537134369285	24.25588436928702
16	20.698840265082268	25.2647014015844	29.288067489335774	24.748390843997562
17	21.86297893814747	25.94038029402803	28.080010283363805	24.116630484460693
18	22.56008150517977	23.90477414686167	30.048131855575868	23.487012492382693
19	22.50449897166362	25.179601805301644	27.96420342017063	24.351695802864107
20	23.99832419256551	27.670342017062765	28.39041552407069	19.940918266301036
21	24.7770747638635	26.12748038543571	29.494686928702013	19.60075792199878
22	22.547822402498475	23.901917656916513	29.296517938756857	24.253742001828154
23	22.844064213893965	23.645309643510053	28.93445783820841	24.57616830438757
24	24.04200468464351	24.406445193479588	27.236512606642293	24.315037515234614
25	21.269900213284583	26.182705857708715	26.89492401736746	25.65246991163924
26	19.70978062157221	26.213770185862277	28.473372752894576	25.60307643967093
27	21.074944774527726	26.27089998476539	29.11834437842779	23.535810862279096
28	20.31238097958562	25.09688261730652	28.648689823278488	25.94204657982937
29	21.918085390006095	25.77006208104814	28.223191841864715	24.08866068708105
30	23.62888482632541	27.697359651127364	27.579172379646554	21.094583142900667
31	23.98059015082267	27.05393529098111	27.28578705819622	21.6796875
32	24.570931406154784	26.77399927635588	24.884788238878734	23.7702810786106
33	25.543090150822668	25.69424607708714	25.368487202925046	23.394176569165143
34	21.804420894271786	23.455829143814746	30.658111479280926	24.081638482632542
35	22.113397890006095	25.807791552407068	27.039176759597805	25.03963379798903
36	25.022851919561244	27.12939423369896	26.334575906459477	21.51317794028032
37	23.794799283973187	27.066789495734305	27.398499390615477	21.739911829677023
38	24.550578915295553	26.928011692565505	25.429187614259597	23.09222177787934
39	24.197207304996954	25.032849634369285	27.28626313985375	23.483679920780013
40	23.933458066727606	23.736241240097502	28.621672189213893	23.708628503961
41	20.481628008836076	24.133650403717247	27.330776774832422	28.05394481261426
42	22.975343730956734	23.490345063985373	27.776627247105424	25.757683957952466
43	24.887644728823886	22.14041552407069	28.490511692565505	24.481428054539915
44	23.60793723339427	22.676721511273616	27.36065089884217	26.354690356489947
45	23.163753046922608	22.17112279098111	26.43824268738574	28.22688147471054
46	22.470221092321754	23.236117458866545	29.414348148994517	24.879313299817184
47	19.57183596130408	24.60794675502742	31.367830210237663	24.452387073430835
48	20.786558310481414	24.446912134369285	29.665838284582573	25.100691270566728
49	23.860022471054236	21.8814271023766	31.23286106032907	23.025689366240098
50	23.426550121876904	21.948792656916513	28.34233127666057	26.28232594454601
51	21.24621515082267	21.83441403869592	26.836961075563682	30.082409734917732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	573.0
2	970.0
3	1985.5
4	3001.0
5	2111.5
6	1222.0
7	1620.0
8	2018.0
9	2096.5
10	2175.0
11	2183.0
12	2191.0
13	2209.0
14	2227.0
15	2292.0
16	2357.0
17	2316.0
18	2275.0
19	2424.0
20	2573.0
21	2851.5
22	3130.0
23	3359.5
24	3589.0
25	4351.0
26	5895.5
27	6678.0
28	7529.0
29	8380.0
30	9528.0
31	10676.0
32	12577.0
33	14478.0
34	16804.5
35	19131.0
36	20837.0
37	22543.0
38	24424.5
39	26306.0
40	29593.5
41	32881.0
42	37194.5
43	41508.0
44	46599.5
45	51691.0
46	62502.5
47	73314.0
48	88693.0
49	104072.0
50	99875.5
51	95679.0
52	78370.0
53	61061.0
54	55252.0
55	49443.0
56	46925.0
57	44407.0
58	41941.5
59	39476.0
60	34999.0
61	30522.0
62	26575.0
63	22628.0
64	19552.5
65	16477.0
66	14162.0
67	11847.0
68	10134.5
69	8422.0
70	7296.0
71	6170.0
72	5406.5
73	4643.0
74	3551.0
75	1881.0
76	1303.0
77	944.5
78	586.0
79	404.5
80	223.0
81	152.0
82	81.0
83	70.5
84	60.0
85	40.0
86	20.0
87	11.5
88	3.0
89	4.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	840192.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.26668728080695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.93385203139535	24.64934812463664
2	11.169481285333811	7.654822445867186
3	3.931820146632052	4.041913542270512
4	1.9915053579442807	2.729691652749127
5	1.2575212679529348	2.1545544018953535
6	0.9438990857215217	1.9406576877035384
7	0.7761722662074592	1.861779662551637
8	0.6637940700221625	1.819681905304281
9	0.6328676366667565	1.9517649656222786
>10	6.542055299606786	39.58230106736091
>50	0.0967426612928531	2.279457448995487
>100	0.050123903750248694	3.2289305434946
>500	0.005608268951076177	1.2220042164011502
>1k	0.0035051680944226107	2.1717179316639665
>5k	0.0010515504283267832	2.7113744034833456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	8766	1.0433329524680073	No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	8495	1.0110784201706275	No Hit
TCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	5313	0.6323554616087751	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	4373	0.520476272090189	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTG	2482	0.2954086684948202	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	1877	0.223401317794028	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC	1765	0.21007103138330285	No Hit
TCCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTG	1712	0.20376294942108472	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1504	0.17900670322973797	No Hit
TTCCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	1190	0.1416342931139549	No Hit
GCCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTG	1113	0.13246972120658135	No Hit
TGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTG	1038	0.12354319012797074	No Hit
TTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTG	1027	0.12223396556977452	No Hit
CGTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	958	0.11402155697745277	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1902041438147471E-4	0.0	0.0	1.0607099329677026	0.0
2	1.1902041438147471E-4	0.0	0.0	3.4250504646556976	0.0
3	1.1902041438147471E-4	0.0	0.0	4.667504570383913	0.0
4	1.1902041438147471E-4	0.0	0.0	5.90615002285192	0.0
5	1.1902041438147471E-4	0.0	0.0	7.944731680377818	0.0
6	1.1902041438147471E-4	0.0	0.0	9.078996229433272	0.0
7	1.1902041438147471E-4	0.0	0.0	10.083171465569775	0.0
8	1.1902041438147471E-4	0.0	0.0	11.383231451858622	0.0
9	1.1902041438147471E-4	0.0	0.0	12.023561281230958	0.0
10	1.1902041438147471E-4	0.0	0.0	13.207576363497868	0.0
11	1.1902041438147471E-4	0.0	0.0	15.393862355271176	0.0
12	1.1902041438147471E-4	0.0	0.0	17.254389472882387	0.0
13	1.1902041438147471E-4	0.0	0.0	17.935067222730044	0.0
14	1.1902041438147471E-4	0.0	0.0	18.22785744210847	0.0
15	1.1902041438147471E-4	0.0	0.0	18.64038219835466	0.0
16	1.1902041438147471E-4	0.0	0.0	19.524465836380255	0.0
17	1.1902041438147471E-4	0.0	0.0	20.713955857708715	0.0
18	1.1902041438147471E-4	0.0	0.0	21.925464655697745	0.0
19	1.1902041438147471E-4	0.0	0.0	22.788481680377817	0.0
20	1.1902041438147471E-4	0.0	0.0	23.74576287324802	0.0
21	1.1902041438147471E-4	0.0	0.0	24.89109632084095	0.0
22	1.1902041438147471E-4	0.0	0.0	26.095820955210236	0.0
23	1.1902041438147471E-4	0.0	0.0	27.127965988726388	0.0
24	1.1902041438147471E-4	0.0	0.0	27.940875418951858	0.0
25	1.1902041438147471E-4	0.0	0.0	28.636430720597197	0.0
26	1.1902041438147471E-4	0.0	0.0	29.280331162400977	0.0
27	1.1902041438147471E-4	0.0	0.0	29.955772014015842	0.0
28	1.1902041438147471E-4	0.0	0.0	30.606218578610605	0.0
29	1.1902041438147471E-4	0.0	0.0	31.29760816575259	0.0
30	1.1902041438147471E-4	0.0	0.0	32.03839122486289	0.0
31	1.1902041438147471E-4	0.0	0.0	32.67550750304692	0.0
32	1.1902041438147471E-4	0.0	0.0	33.269657411639244	0.0
33	1.1902041438147471E-4	0.0	0.0	33.91034430225472	0.0
34	1.1902041438147471E-4	0.0	0.0	34.58804654174284	0.0
35	1.1902041438147471E-4	0.0	0.0	35.23337522851919	0.0
36	1.1902041438147471E-4	0.0	0.0	35.83538048446069	0.0
37	1.1902041438147471E-4	0.0	0.0	36.44036125076173	0.0
38	1.1902041438147471E-4	0.0	0.0	37.05831524223035	0.0
39	1.1902041438147471E-4	0.0	0.0	37.90931120505789	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.032201E-4	45.000004	13
TTATGCG	20	7.032201E-4	45.000004	1
CGGTCTA	20	7.032201E-4	45.000004	31
TACGGGA	95	0.0	42.63158	4
GCGCGAC	155	0.0	42.09677	9
CGCGACC	160	0.0	40.781254	10
CGCATAC	40	3.4567347E-7	39.375004	26
TCGTTTG	315	0.0	39.285713	1
CGTTTGG	490	0.0	39.030613	2
CGTTTTT	1345	0.0	38.64312	1
GACCGAT	315	0.0	38.57143	9
GTTTGCG	140	0.0	38.571426	1
TTTGGGA	4640	0.0	38.162716	4
TTGGGAT	2385	0.0	37.924526	5
GAATTCG	280	0.0	37.767857	12
TTTGGGC	1600	0.0	37.125	4
ACGGGAT	85	0.0	37.058823	5
GGGCGAT	1300	0.0	37.038464	7
TTTCTTG	1320	0.0	36.988636	1
TTTTGCG	700	0.0	36.964287	1
>>END_MODULE