FastQCFastQC Report
Sat 14 Jan 2017
SRR2933933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665402
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC56340.8467062016645577No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC48240.7249752781025606No Hit
GAATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC43150.6484801668765648No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG42350.6364573596111824No Hit
TCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC40540.6092557581732547No Hit
CTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT33220.4992470716950055No Hit
GCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC33200.4989465015133709No Hit
TCCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG17790.26735717656394176No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.19627232860736815No Hit
CGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG12630.1898100697022251No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCT10630.15975305153876904No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTC10460.15719820499487527No Hit
GAACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT10210.15344107772444326No Hit
TGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG9710.14592682318357925No Hit
TTCCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT9710.14592682318357925No Hit
GCCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG9360.14066684500497445No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCGATCAT9310.13991541955088801No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCGATCATCGTA7970.11977721738137247No Hit
TTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG7130.10715326975272092No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC6730.1011418661200297No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT207.031142E-445.00000413
GATCCGA207.031142E-445.00000429
CGACCTT207.031142E-445.00000441
GTTCGCT207.031142E-445.00000421
ACGGAGT207.031142E-445.00000423
TCCACGC207.031142E-445.00000444
ATAAGCG207.031142E-445.00000433
CGCAGAT207.031142E-445.00000413
ACCTTCG207.031142E-445.00000414
CGAACGA253.889029E-545.022
ACGGAAC253.889029E-545.014
CGATCTA351.2109012E-745.011
ACGAACG253.889029E-545.021
GTATTCG302.164119E-644.9999961
TCGCGTG302.164119E-644.9999961
TTTGTCG1250.043.1999971
TTCGGGT1200.041.2499964
GACCGAT2650.040.754729
TTTCGCG2350.040.2127651
GTTTGCG1350.040.01
Produced by FastQC (version 0.11.5)