##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933933.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665402
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30834442938254	33.0	31.0	34.0	31.0	34.0
2	32.534882672429596	34.0	31.0	34.0	31.0	34.0
3	32.60311210366064	34.0	31.0	34.0	31.0	34.0
4	36.01775918918188	37.0	35.0	37.0	35.0	37.0
5	36.047371363476515	37.0	35.0	37.0	35.0	37.0
6	35.58017108454739	37.0	35.0	37.0	33.0	37.0
7	35.952224369629185	37.0	35.0	37.0	35.0	37.0
8	35.92283461726896	37.0	35.0	37.0	35.0	37.0
9	37.806706321892634	39.0	38.0	39.0	35.0	39.0
10	37.49601594224244	39.0	37.0	39.0	35.0	39.0
11	37.42465607256967	39.0	37.0	39.0	35.0	39.0
12	37.2914929621492	39.0	37.0	39.0	34.0	39.0
13	37.18878963393558	39.0	37.0	39.0	34.0	39.0
14	38.387595769174126	40.0	38.0	41.0	34.0	41.0
15	38.439185635149876	40.0	38.0	41.0	34.0	41.0
16	38.5463945103862	40.0	38.0	41.0	34.0	41.0
17	38.4860625606776	40.0	38.0	41.0	34.0	41.0
18	38.46073802002399	40.0	38.0	41.0	34.0	41.0
19	38.48535171219804	40.0	38.0	41.0	34.0	41.0
20	38.402467981761404	40.0	37.0	41.0	34.0	41.0
21	38.335842092449376	40.0	37.0	41.0	34.0	41.0
22	38.322843934944586	40.0	37.0	41.0	34.0	41.0
23	38.2734737797602	40.0	37.0	41.0	34.0	41.0
24	38.2245965596737	40.0	37.0	41.0	34.0	41.0
25	38.13039035049489	40.0	37.0	41.0	34.0	41.0
26	38.06707824743538	40.0	37.0	41.0	34.0	41.0
27	38.04428901626385	40.0	37.0	41.0	34.0	41.0
28	38.00292154216549	40.0	37.0	41.0	34.0	41.0
29	37.965813147540885	40.0	37.0	41.0	34.0	41.0
30	37.87454350903664	40.0	36.0	41.0	33.0	41.0
31	37.84369448844458	40.0	36.0	41.0	33.0	41.0
32	37.74530584518832	40.0	36.0	41.0	33.0	41.0
33	37.640746496103105	40.0	36.0	41.0	33.0	41.0
34	37.65844857695048	40.0	36.0	41.0	33.0	41.0
35	37.62744776841669	40.0	36.0	41.0	33.0	41.0
36	37.501777872624366	40.0	36.0	41.0	33.0	41.0
37	37.4594906537702	40.0	36.0	41.0	33.0	41.0
38	37.4209665735901	40.0	36.0	41.0	33.0	41.0
39	37.370216801272015	40.0	36.0	41.0	33.0	41.0
40	37.26449725128569	39.0	35.0	41.0	32.0	41.0
41	37.17390840424285	39.0	35.0	41.0	32.0	41.0
42	37.177976621651275	39.0	35.0	41.0	32.0	41.0
43	37.067429914547894	39.0	35.0	41.0	32.0	41.0
44	36.96185914680148	39.0	35.0	41.0	31.0	41.0
45	36.988933005912216	39.0	35.0	41.0	32.0	41.0
46	36.89624016759793	39.0	35.0	41.0	31.0	41.0
47	36.76227603764341	39.0	35.0	41.0	31.0	41.0
48	36.752074685678735	39.0	35.0	41.0	31.0	41.0
49	36.77592643244234	39.0	35.0	41.0	31.0	41.0
50	36.683872305764034	39.0	35.0	40.0	31.0	41.0
51	35.699928764866954	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	10.0
10	19.0
11	15.0
12	16.0
13	15.0
14	18.0
15	22.0
16	24.0
17	44.0
18	79.0
19	158.0
20	306.0
21	475.0
22	729.0
23	1164.0
24	1660.0
25	2496.0
26	3510.0
27	4315.0
28	5025.0
29	6315.0
30	7998.0
31	10573.0
32	14230.0
33	20539.0
34	36743.0
35	52102.0
36	48691.0
37	77617.0
38	151025.0
39	219338.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.818383473449135	4.6565835389734325	55.126825588140704	16.398207399436732
2	18.67788194204406	7.894776390813372	51.411327287865085	22.01601437927749
3	19.30487134093375	7.698053206933553	50.30868557653869	22.688389875594
4	19.114159560686623	4.449941539099672	51.81003363380332	24.62586526641038
5	20.07132530410188	5.123819886324357	49.80538080739162	24.99947400218214
6	20.367988073375194	8.738476890661586	51.87916477557928	19.01437026038395
7	78.20956354203925	2.62593139185034	13.245677049362643	5.918828016747771
8	78.5404913120189	5.278012389502887	9.281456923784418	6.900039374693794
9	72.66163912942852	5.390125067252578	12.083372156981795	9.864863646337103
10	37.25958142596505	24.778104063408286	20.361676099560867	17.60063841106579
11	26.386905960607272	24.482343004679876	28.61638528288163	20.514365751831225
12	25.006988256723005	21.073426289671506	31.90853048232497	22.01105497128052
13	22.130080763207804	24.33581504113303	33.66160606670854	19.872498128950618
14	17.476653211141535	27.775540199758943	31.710755302809428	23.03705128629009
15	14.886790241087342	24.333410479679955	38.89994319223567	21.879856086997034
16	16.65474404946183	24.42929236762138	33.08421074778855	25.831752835128235
17	16.819757079179205	25.058836613054964	30.956173861815866	27.165232445949965
18	18.362884391691036	24.146455826703257	32.771016618525344	24.719643163080363
19	20.29029068142266	25.558985395294876	30.11532877869318	24.035395144589287
20	22.03314687963066	25.358955939417072	32.1183284691059	20.489568711846374
21	22.169154886820298	27.108875536893486	30.583917691861462	20.138051884424755
22	20.12377480079711	24.408853595270227	30.092184874707318	25.37518672922534
23	19.310281604203173	26.410651004956403	28.93213425868873	25.346933132151694
24	21.276762017547288	23.581233600139466	29.893057129374423	25.248947252938827
25	18.178634870349054	27.674849188911367	27.867214105157483	26.279301835582096
26	17.338541212680454	28.251493082377273	28.3395601455962	26.070405559346078
27	18.06547019696364	28.488041815323665	27.785459015752885	25.66102897195981
28	16.452911172494222	25.16418646171788	31.13320969879862	27.24969266698928
29	19.322304411468554	24.202512165578103	28.94596048704392	27.52922293590942
30	19.095975064697733	27.55732624789225	28.205806414768812	25.140892272641203
31	20.014216969591313	27.645543596201993	25.911103363079764	26.42913607112693
32	20.84168667963126	28.82873811620644	24.440563749432673	25.889011454729623
33	19.538264086972987	28.338057294688024	24.45454026287868	27.66913835546031
34	17.936976444314865	26.574612039038055	28.430783195722288	27.057628320924792
35	17.90676914106059	26.359403788987713	26.086335778972714	29.64749129097899
36	18.883171376100464	28.94520906158984	25.87623722201015	26.29538234029955
37	18.602589111544603	25.542454035304974	29.062431432427317	26.792525420723113
38	19.585904460762066	26.720088006949183	25.897727989997026	27.796279542291728
39	21.175019011063988	26.933192265728085	29.104811828037786	22.786976895170138
40	20.19245508730061	25.820180883135308	30.05596616782035	23.93139786174373
41	17.20568919239798	26.36556547771122	28.507578877129912	27.921166452760886
42	18.780226088890625	25.564395658564298	26.83595781196931	28.819420440575772
43	21.267143771734982	25.357302803418087	26.45227997511279	26.923273449734147
44	20.15473352950547	23.72565757241487	28.076861806847592	28.042747091232066
45	20.251667413082618	23.24053729925669	26.577467455763582	29.93032783189711
46	21.783072488510705	24.581380879528467	28.42762720881512	25.207919423145707
47	16.583659201505256	24.232118328469106	32.76710920616409	26.41711326386155
48	17.36423996321021	23.966865143176605	30.014036627482334	28.65485826613085
49	19.29344967403164	21.98445451020586	32.51447996850024	26.207615847262254
50	20.16525348586268	21.830262007027333	30.193026170645716	27.811458336464273
51	18.208691888512508	21.6386485162353	27.032680995849123	33.11997859940307
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	136.0
1	347.0
2	558.0
3	1432.0
4	2306.0
5	1563.5
6	821.0
7	916.0
8	1011.0
9	1067.5
10	1124.0
11	1192.5
12	1261.0
13	1167.0
14	1073.0
15	1017.0
16	961.0
17	1137.0
18	1313.0
19	1301.0
20	1289.0
21	1641.0
22	1993.0
23	2381.0
24	2769.0
25	3160.5
26	4315.0
27	5078.0
28	6435.5
29	7793.0
30	9300.0
31	10807.0
32	11526.5
33	12246.0
34	13822.0
35	15398.0
36	17504.0
37	19610.0
38	21619.0
39	23628.0
40	26894.5
41	30161.0
42	34693.5
43	39226.0
44	44605.5
45	49985.0
46	67284.0
47	84583.0
48	87358.5
49	90134.0
50	85010.5
51	79887.0
52	65282.0
53	50677.0
54	42176.5
55	33676.0
56	29058.5
57	24441.0
58	22179.5
59	19918.0
60	18057.0
61	16196.0
62	14145.5
63	12095.0
64	9930.0
65	7765.0
66	6207.5
67	4650.0
68	3636.5
69	2623.0
70	2270.0
71	1917.0
72	1504.5
73	1092.0
74	999.5
75	605.0
76	303.0
77	281.0
78	259.0
79	175.0
80	91.0
81	80.5
82	70.0
83	41.0
84	12.0
85	7.0
86	2.0
87	1.5
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	665402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.589073995170605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.54343652597409	19.68958667259986
2	13.629336841920491	8.065589126413807
3	5.8474419628595005	5.190611787645463
4	3.1027452521329897	3.6722943541410924
5	1.8152184901856712	2.685531711175285
6	1.2042667062944108	2.1379882009479423
7	0.8270824876384886	1.7130823448791515
8	0.6083996910380328	1.4401586781410631
9	0.43150409597760364	1.1491025962590462
>10	5.67839216111697	39.28802488957684
>50	0.2259166864134252	4.23762218669852
>100	0.07547671114229501	4.094509574203125
>500	0.0041075761165874835	0.9870516382916292
>1k	0.006161364174881227	4.792905200065309
>5k	5.134470145734354E-4	0.8559410389618345
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC	5634	0.8467062016645577	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC	4824	0.7249752781025606	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC	4315	0.6484801668765648	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG	4235	0.6364573596111824	No Hit
TCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC	4054	0.6092557581732547	No Hit
CTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT	3322	0.4992470716950055	No Hit
GCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC	3320	0.4989465015133709	No Hit
TCCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG	1779	0.26735717656394176	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1306	0.19627232860736815	No Hit
CGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG	1263	0.1898100697022251	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCT	1063	0.15975305153876904	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTC	1046	0.15719820499487527	No Hit
GAACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT	1021	0.15344107772444326	No Hit
TGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG	971	0.14592682318357925	No Hit
TTCCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT	971	0.14592682318357925	No Hit
GCCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG	936	0.14066684500497445	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCGATCAT	931	0.13991541955088801	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCGATCATCGTA	797	0.11977721738137247	No Hit
TTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG	713	0.10715326975272092	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC	673	0.1011418661200297	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5192349887737037	0.0
2	0.0	0.0	0.0	2.6190182776727453	0.0
3	0.0	0.0	0.0	3.875401636905209	0.0
4	0.0	0.0	0.0	5.186939624467615	0.0
5	0.0	0.0	0.0	7.655823096413897	0.0
6	1.5028509081728037E-4	0.0	0.0	8.844427879687768	0.0
7	1.5028509081728037E-4	0.0	0.0	10.03844292623106	0.0
8	1.5028509081728037E-4	0.0	0.0	11.793923072067713	0.0
9	1.5028509081728037E-4	0.0	0.0	12.565336443232813	0.0
10	1.5028509081728037E-4	0.0	0.0	14.609063393257008	0.0
11	1.5028509081728037E-4	0.0	0.0	17.250474149461528	0.0
12	1.5028509081728037E-4	0.0	0.0	20.384519433365092	0.0
13	1.5028509081728037E-4	0.0	0.0	21.239791885206238	0.0
14	1.5028509081728037E-4	0.0	0.0	21.562003119918487	0.0
15	1.5028509081728037E-4	0.0	0.0	22.12436992975675	0.0
16	1.5028509081728037E-4	0.0	0.0	23.214838548726934	0.0
17	1.5028509081728037E-4	0.0	0.0	24.66839594711167	0.0
18	1.5028509081728037E-4	0.0	0.0	26.269082449406525	0.0
19	1.5028509081728037E-4	0.0	0.0	27.47451916285193	0.0
20	1.5028509081728037E-4	0.0	0.0	28.631564077054172	0.0
21	1.5028509081728037E-4	0.0	0.0	30.12254246305241	0.0
22	1.5028509081728037E-4	0.0	0.0	31.61006429196185	0.0
23	1.5028509081728037E-4	0.0	0.0	32.893498967541426	0.0
24	1.5028509081728037E-4	0.0	0.0	33.94098605053787	0.0
25	1.5028509081728037E-4	0.0	0.0	34.85276569652631	0.0
26	1.5028509081728037E-4	0.0	0.0	35.65348466040078	0.0
27	1.5028509081728037E-4	0.0	0.0	36.439625970465976	0.0
28	1.5028509081728037E-4	0.0	0.0	37.25281859687828	0.0
29	1.5028509081728037E-4	0.0	0.0	38.09546710109077	0.0
30	1.5028509081728037E-4	0.0	0.0	39.019570124526226	0.0
31	1.5028509081728037E-4	0.0	0.0	39.91061042798188	0.0
32	1.5028509081728037E-4	0.0	0.0	40.72801103693707	0.0
33	1.5028509081728037E-4	0.0	0.0	41.47733249975203	0.0
34	1.5028509081728037E-4	0.0	0.0	42.241832756739534	0.0
35	1.5028509081728037E-4	0.0	0.0	43.037592312617036	0.0
36	1.5028509081728037E-4	0.0	0.0	43.810658819781125	0.0
37	1.5028509081728037E-4	0.0	0.0	44.59619898948305	0.0
38	1.5028509081728037E-4	0.0	0.0	45.344167886480655	0.0
39	1.5028509081728037E-4	0.0	0.0	46.1107721347396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACT	20	7.031142E-4	45.000004	13
GATCCGA	20	7.031142E-4	45.000004	29
CGACCTT	20	7.031142E-4	45.000004	41
GTTCGCT	20	7.031142E-4	45.000004	21
ACGGAGT	20	7.031142E-4	45.000004	23
TCCACGC	20	7.031142E-4	45.000004	44
ATAAGCG	20	7.031142E-4	45.000004	33
CGCAGAT	20	7.031142E-4	45.000004	13
ACCTTCG	20	7.031142E-4	45.000004	14
CGAACGA	25	3.889029E-5	45.0	22
ACGGAAC	25	3.889029E-5	45.0	14
CGATCTA	35	1.2109012E-7	45.0	11
ACGAACG	25	3.889029E-5	45.0	21
GTATTCG	30	2.164119E-6	44.999996	1
TCGCGTG	30	2.164119E-6	44.999996	1
TTTGTCG	125	0.0	43.199997	1
TTCGGGT	120	0.0	41.249996	4
GACCGAT	265	0.0	40.75472	9
TTTCGCG	235	0.0	40.212765	1
GTTTGCG	135	0.0	40.0	1
>>END_MODULE