FastQCFastQC Report
Sat 14 Jan 2017
SRR2933932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2387511
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276281.1571883857289034No Hit
CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC111380.46651093963546136No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT95870.4015478881563268Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG81570.3416528761542879No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT73810.3091504081028318No Hit
CGTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC73640.30843836949861175No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT57530.24096224059281823No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC49880.20892050340291626No Hit
TCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC49770.2084597725413621No Hit
CGTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT49230.20619800285736906No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCC31850.133402526731814No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTC28000.11727694657741891No Hit
TCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTG24800.10387386696857102No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTA207.034815E-445.013
CGTTTTT148550.043.9851881
CGTTTCT10400.038.5096131
CGTTTTC7550.038.1456951
CCATACG1600.037.968752
TGGGCGA29650.037.715016
TTTGGGA136700.037.527434
TTGGGAC56100.037.419795
GTTTTTT178950.037.0913662
TTGGGAT81150.036.987065
TTTCTCG10600.036.9339641
GCGATCG1050.036.4285749
CGTTCTG9550.036.0471231
ATAGGCG250.002107562336.013
AATCGTT751.8189894E-1236.022
TAGCGGG4700.035.9042553
TGCGGGA33550.035.8122184
GGGCGAT48700.035.8059547
TCTTGCG8500.035.7352941
TTTGGGC48000.035.6718754
Produced by FastQC (version 0.11.5)